液相色谱-串联质谱法测定银杏叶提取物及含银杏叶提取物的保健食品中咖啡因的含量

目的建立一种液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS)测定银杏叶提取物及含银杏叶提取物的保健食品中咖啡因的含量的方法。方法样品经甲醇超声提取, C_(18)色谱柱分离,以0.1%甲酸水溶液-0.1%甲酸乙腈溶液(50:50, V:V)为流动相进行等度洗脱,在正离子模式下检测,外标法定量。结果该方法在21.5～1075 ng/mL浓度范围内呈现良好的线性关系,相关系数r为0.9999,平均回收率为97.20%～99.63%,相对标准偏差(relative standard deviation, RSD)小于2.67%。在所检测的6批银杏叶提取物及1批保健食品中均检测出咖啡因,含量范围为0.41～8.71mg/kg。结论该方法专属性强、准确性好、灵敏度高、重现性好,适用于银杏叶提取物及含银杏叶提取物的保健食品中咖啡因的鉴别和含量测定。     

超高效液相色谱-串联质谱法测定猪肉中肾上腺素残留量

建立了猪肉中肾上腺素残留量的超高效液相色谱-串联质谱(UPLC-MS/MS)的检测方法。样品经乙腈+甲醇(95+5)提取,C_(18)色谱柱分离,超高效液相色谱-串联质谱仪检测。本方法定量限为10μg/kg,线性范围为5～500ng/mL。样品加标回收率为75%～98%,相对标准偏差为4.8%～7.9%,方法简便快速、灵敏度高,为猪肉中肾上腺素残留量测定提供了新的方法。     

高效液相色谱-串联质谱法测定蜂胶软胶囊中 黄曲霉毒素B1

目的建立测定蜂胶软胶囊中黄曲霉毒素B1的高效液相色谱-串联质谱法。方法样品经甲醇超声提取,用氰基柱将黄曲霉毒素B1分离,用高效液相色谱-串联质谱进行测定,外标法定量。结果黄曲霉毒素B1标准曲线在0.1~2.0 ng/m L范围内有良好的线性,r0.9998,回收率为80.1%~87.8%,RSD3.0%,检出限为1.0μg/kg。结论该方法结果准确可靠,较节省试剂、耗材,节省样品前处理时间,有利于降低检验成本,适用于保健食品蜂胶软胶囊中黄曲霉毒素B1的限量测定。     

固相萃取-高效液相色谱-串联质谱联用法检测辣椒及其制品和肉酱中的罗丹明B含量

建立了辣椒及其制品和肉酱中罗丹明B含量的固相萃取-高效液相色谱-串联质谱联用(SPELC-MS/MS)检测方法。样品经乙腈提取后,经中性氧化铝固相萃取柱净化,超高效液相色谱-串联质谱法检测。本方法定量限为0.1μg/kg,线性范围为0.2~50ng/mL;在罗丹明B添加水平为0.1~10μg/kg时,辣椒片样品中的回收率为76%~92%,辣椒制品中的回收率为74%~93%,肉酱样品中的回收率为70%~90%。     

多种蜂胶中总黄酮检测方法的适用性研究

目的通过3种分光光度法之间差异的比较和适用性考量,筛选出适合检测蜂胶中总黄酮含量的分光光度法。方法采用同批次样品,对蜂胶(国产蜂胶和巴西绿蜂胶)中总黄酮检测方法进行回收率和含量测定,并对结果进行分析。结果巴西绿蜂胶粉3种方法的检测结果为4.99~9.34 g/100 g;国产蜂胶粉3种方法的检测结果为7.82~17.97 g/100 g,其RSD为24.2%~31.2%;可见3种方法测定结果偏差较大。结论 GB/T20574-2006的方法最适合蜂胶中总黄酮的检测。     

超高液相色谱串联质谱法检测土豆及土豆制品中α-茄碱

目的建立超高效液相色谱串联质谱法检测土豆及其制品中α-茄碱的分析方法。方法通过优化提取液,土豆样品经1%甲酸-甲醇(1:1,V:V)超声提取,采用BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm)分离,梯度洗脱,采用超高效液相色谱串联质谱法,在多反应监测模式进行定性和定量分析,而土豆淀粉用MCX固相萃取柱进行净化,液相色谱串联质谱法测定。结果土豆样品中回收率为75%~115%,相对标准偏差为5.0%~8.6%,土豆淀粉的回收率范围为67%~100%,相对标准偏差为3.4%~11.3%,方法的检出限和定量限分别为0.5 mg/kg和1.0 mg/kg。结论该方法适合于土豆及其制品中α-茄碱的分析。     

QuEChERS/超高效液相色谱-串联质谱法检测辣椒制品中的罗丹明B

采用基质固相分散技术(QuEChERS)为样品前处理方法,建立了超高效液相色谱-串联质谱(UPLC-MS/MS)法检测辣椒制品中罗丹明B含量的分析方法。样品用乙腈提取,氧化铝吸附剂净化后,经电喷雾电离,采用多反应监测模式进行检测,外标法定量。结果表明,添加浓度为5-100ng/m L时,罗丹明B的回收率为88.4-103.2%。方法简便快捷,重现性好,可用于辣椒制品中罗丹明B含量的测定。     

MAX固相萃取-UPLC-MS/MS法测定肉类及其制品中L-谷氨酸钠含量的方法

目的：建立MAX固相萃取-超高效液相色谱/串联质谱法检测肉类及其制品中L-谷氨酸钠含量的方法。方法：样品经盐酸水溶液萃取,上清液经活化MAX柱净化提取,2%甲酸(v/v)的甲醇洗脱液氮吹、盐酸水溶液复溶,初始流动相稀释100倍,经0.22μm滤膜过滤后,经超高效液相色谱/串联质谱的ESI正离子模式和MRM模式检测。结果：谷氨酸钠在0.01～1.00 mg·L^-1分离效果好,相关系数为0.997 7,检出限为15 mg·kg^-1,定量限为50 mg·kg^-1,平均加标回收率为89.04%,相对标准偏差为2.28%。结论：该方法操作简便、快捷、灵敏,具有较好的准确度和精密度,可满足肉类及其制品中谷氨酸钠含量的测定。     

固相萃取-高效液相色谱-串联质谱法测定蜂胶中氯霉素残留

该文建立固相萃取结合高效液相色谱-串联质谱技术(solid phase extraction-high performance liquid chromatography-tandem mass spectrometry,SPE-HPLC-MS/MS)测定蜂胶中氯霉素残留的检测方法.蜂胶样品经乙腈溶解,氢氧化钙溶液沉淀...     

高效液相色谱- 电喷雾三级四极杆质谱测定苹果、山楂、番茄制品中棒曲霉素的研究

建立了苹果、山楂、番茄制品中棒曲霉素总量的提取方法和液相色谱串联质谱确证方法。清汁样品用乙腈稀释后直接过MycoSep 228 真菌霉素多功能净化柱;浊汁、固体样品用果胶酶水解后,再用乙酸乙酯提取样品中棒曲霉素,浓缩后经MycoSep 228 柱净化,用Atlantis dC18 色谱柱分离,液相色谱串联质谱测定,电喷雾负离子(ESI －)多反应模式下监测;实验结果表明,样品中棒曲霉素浓度在1.0～200μg/kg 范围内时与其峰面积成良好线性关系,检测低限为2.0μg/kg,3 个加标水平下回收率为76.0%～95.3%,相对标准偏差为5.4%～11.2%。该方法选择性好,灵敏度高,定性可靠,可满足苹果、山楂、番茄制品中棒曲霉素测定的要求。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press