凤尾鱼酶解产物脱腥脱色工艺优化

凤尾鱼酶解物(AFH)通过大孔树脂吸附处理后,发现DA201-C型大孔树脂为最佳吸附材料。在单因素实验基础上,以腥味、色泽和回收率为评价指标,运用正交实验L₉(34)优化了DA201-C型大孔树脂对凤尾鱼酶解产物脱腥脱色工艺,得到最佳条件为:pH6,大孔树脂量5%,脱腥脱色时间1.5 h,以及温度25℃,该条件下感官评定综合评分为84.36。同时,研究表明脱腥脱色后,凤尾鱼酶解产物中牛磺酸、丙氨酸、甲硫氨酸和异亮氨酸呈显著性降低(P<0.01),挥发性物质成分中醛和含氮类化合物相对含量呈显著性降低。     

大孔吸附树脂静态处理对鳀鱼酶解液ACE抑制活性的影响

应用六种大孔吸附树脂静态处理鳀鱼酶解液,采用改进的高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定其ACE抑制活性.比较了六种树脂对鳀鱼酶解液ACE抑制活性的影响.结果表明:树脂吸附导致酶解液ACE抑制活性的变化主要与树脂的极性有关,非极性树脂会降低其ACE抑制活性,极性和弱极性树脂ACE抑制活性稍有提高.树脂H103处理后的ACE抑制活性降低最明显,IC50值由9.10mg提高到了17.58mg.     

大孔树脂富集发酵大豆提取物中乙酰胆碱酯酶抑制剂的工艺研究

为了确定适宜的大孔树脂型号及富集乙酰胆碱酯酶抑制剂的最佳工艺条件,通过静态吸附率和解吸液抑制率的测定,确定较优树脂型号,并考察动态解吸的相关影响因素。实验结果表明:ADS-17为富集乙酰胆碱酯酶抑制剂的适宜树脂,其静态吸附率和解吸液抑制率分别可达96.65%、64.24%,其适宜富集工艺条件:静态吸附饱和的树脂用80%乙醇溶液进行动态解吸,洗脱速度为1.0mL/min时,解吸液AChE抑制率为62.64%。     

大孔树脂对酶解大豆肽脱盐效果影响及其最佳条件研究

本文旨在研究不同种类大孔树脂对酶解大豆肽吸附和解吸过程中脱盐效果的影响及其最佳条件。以酶解大豆肽为研究对象,首先以DA201-C、AB-8、D3520、D4006和NAX-Ⅱ5种不同的大孔吸附树脂进行酶解大豆肽的脱盐效果研究,得出DA201-C树脂对酶解液的脱盐效果最好;并对DA201-C树脂进行静态吸附解析工艺的优化并测定其动态吸附解吸实验,得出最佳条件为:pH4.5,酶解液浓度为60mg/mL,75%乙醇洗脱,脱盐率达到93.23%,肽回收率为88.5%。     

菲律宾蛤仔蒸煮液多糖回收工艺及微胶囊化

目的 回收菲律宾蛤仔蒸煮液中的多糖并采用微胶囊包埋技术对多糖进行脱腥和保护。方法 采用超滤醇沉工艺回收菲律宾蛤仔蒸煮液多糖, 以该多糖为芯材, 大豆蛋白和麦芽糊精为壁材, 采用喷雾干燥法制备微胶囊, 通过单因素和正交试验优化制备工艺。结果 菲律宾蛤仔蒸煮液多糖回收工艺为: 蒸煮液固形物含量调整为10%, 3万分子量超滤膜除杂, 透过液加入3倍体积乙醇, 室温醇沉24 h, 沉淀冻干得菲律宾蛤仔蒸煮液多糖粗品, 总糖含量大于80%, 回收率高于70%; 喷雾干燥法制备菲律宾蛤仔蒸煮液多糖微胶囊工艺参数为: 乳化剂用量0.1%、多糖浓度10 mg/mL、壁材比例3:1、均质时间3 min, 得到的微胶囊的包埋率达到68.63%。结论 超滤醇沉工艺适用于菲律宾蛤仔蒸煮液中多糖的回收, 该多糖采用喷雾干燥法微胶囊化包埋处理, 能够很好地掩盖其腥味, 并起到保护作用。该研究为菲律宾蛤仔蒸煮液的综合开发利用提供思路。     

大孔树脂分离纯化米团花黄色素的研究

为寻找分离纯化米团花黄色素最佳工艺条件,对11种大孔树脂对米团花黄色素的静态吸附、解吸性能进行了比较研究,并确定了最佳吸附树脂D101对米团花黄色素动态吸附、解吸的最佳条件。结果表明:D101型大孔树脂分离纯化米团花黄色素的最佳工艺条件为:上样液浓度0.10~0.13 mg/mL,上样液pH 5,流速为1.5 mL/min;以60%(V/V)的乙醇洗脱,流速为3 mL/min。D101型大孔树脂的饱和吸附量为8.820 mg/g树脂,重复利用10次吸附量仍然很好。采用该工艺分离纯化得到的产品中米团花黄色素的含量为(1.9±0.0055)%,色价为27.93±0.80。     

大孔吸附树脂纯化富集川芎总生物碱的工艺

以盐酸川芎嗪为对照品,采用酸性染料比色法测定川芎总生物碱含量。以吸附率和解吸率为指标,比较7种不同型号大孔吸附树脂的静态吸附与解吸附能力,考察动态吸附与洗脱参数,筛选最佳大孔吸附树脂纯化富集川芎总生物碱的工艺条件。结果表明AB-8型大孔树脂对川芎总生物碱的吸附与解吸性能最好,静态吸附率为75.10%,解吸率为81.45%,静态吸附和解吸时间分别为12h和8h。其动态富集工艺条件为:最佳上样液pH为8.0,上样质量浓度为4.4799mg/mL,上样量为8BV,用13BV去离子水除杂后,再用14BV体积分数85%的乙醇进行洗脱。经AB-8大孔树脂纯化富集后,川芎总生物碱纯度提高了2.38倍,回收率为75.15%(RSD=1.22%,n=3)。     

粉叶爬山虎红色素的提取纯化工艺研究

本文采用酸性乙醇溶液提取粉叶爬山虎果皮中红色素,比较了九种大孔吸附树脂对红色素静态、动态吸附和解吸实验,筛选出红色素纯化的最佳树脂,并用薄层色谱法对红色素进行了单体分离.结果表明:LSA-8大孔吸附树脂纯化红色素效果较好,确定了吸附和洗脱的最佳工艺参数为:上柱液pH6.5～7.5,样液质量浓度0.56mg/mL,吸附流速2.0mL/min;以60%乙醇为解吸剂,洗脱液流速1.0mL/min;通过薄板层析(TLC)研究,得其TLC最佳展层剂是:V(水):V(甲酸):V(乙酸乙酯)=3:2:10,分离红色素,得到4种单体成分.     

响应面法优化大孔树脂吸附发酵羊乳ACE抑制肽

血管紧张素转换酶(ACE)的活性过高是导致高血压的重要因素之一。ACE抑制肽可有效地抑制ACE的活性。为了制备高纯度的ACE抑制肽,考查不同截留分子量超滤膜对发酵羊乳中的ACE抑制肽的分离效果,比较4种树脂(AB-8、HPD-100、DA201-C、DM130)对ACE抑制肽的纯化效果,选择最佳树脂并对其纯化工艺参数进行优化。结果表明,经超滤分离后,ACE抑制肽主要集中在M1 ku组分中,其IC50值降到了0.348 mg/mL,ACE抑制率为86.91%,比超滤前ACE抑制率提高了5.61%;大孔树脂DA201-C最佳,静态吸附最佳工艺条件为上样pH2.15、上样浓度20 mg/mL、吸附率为(72.38±1.26)%,通过大孔树脂后IC_(50)值达到0.301 mg/mL,与超滤液相比IC50降低了0.047 mg/mL。     

大孔吸附树脂分离纯化白骨壤果实中苯乙醇苷的工艺研究

目的:研究利用大孔吸附树脂从白果壤果实中分离纯化苯乙醇苷的工艺。方法:通过静态吸附试验,比较了4种不同类型大孔树脂对白骨壤果实中苯乙醇苷的吸附及解吸附性能,通过动态吸附试验确定了最佳纯化工艺参数。结果:DA-201大孔吸附树脂对苯乙醇苷的吸附和解吸效果较为理想,吸附量和解吸率分别为61.84 mg/g和81.80%。DA-201大孔吸附树脂对苯乙醇苷的最佳动态吸附参数为上样液浓度为6.308 mg/mL,控制上样流速为1.5 BV/h,60%的乙醇溶液解吸DA-201大孔吸附树脂的解吸率最高。放大试验发现,饱和吸附量为44.156 mg/mL,8 BV时基本解吸完成(60%乙醇),获得的苯乙醇苷纯度为38.22%。结论:采用DA-201大孔吸附树脂能够高效分离纯化白骨壤果实中苯乙醇苷。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press