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1.
The processes developed to valorize buttermilks and butter serums generally start by a technological step aiming at removing lipids by centrifugation. The efficiency of this skimming step has never been studied yet. The objective of this study was then to characterize the efficiency of the skimming of industrial buttermilk and butter serum and to determine its consequences on the lipid composition and microstructure of related products. This work clearly shows that the efficiency of the skimming step, operated both at pilot and industrial scales, is never complete and depends on the characteristics of the fluids to be treated. There exists a threshold of lipid content (7% total lipids in dry matter for buttermilk; 20% for butter serum) under which the skimming is not efficient. Above this threshold of lipid content, the skimming step removes all particles with a size larger than 1 μm (large fat globules, butter fines), but does not succeed in removing small size lipid fraction (fragments of membrane, lipid vesicles), which results in an increase in the polar lipids on total lipid ratio for both products (up to 20 ± 5% and 49 ± 6% for buttermilks and butter serums respectively). The difference of skimming efficiency, threshold values of skimming, and composition of final products between the buttermilks and butter serums could be attributed to differences in the microstructure of the lipids. Even if not totally efficient, the skimming step leads to a standardization of total lipid content of buttermilks and butter serums, useful for a better control of technological processes aiming at valorizing individual compounds of these dairy by-products.  相似文献   

2.
《Journal of dairy science》1986,69(2):321-325
A method is described for the isolation of lipids from milk, cream, or buttermilk. Lipid isolation is accomplished by solvent elution of a column containing a mixture of the milk product, anhydrous sodium sulfate, and Celite 545. Total lipids are isolated by elution with a 90:10 mixture of dichloromethane:methanol. Alternatively, lipids may be separated into a neutral lipid fraction by sequential elution of the column with dichloromethane and a polar fraction by elution with a 90:10 mixture of dichloromethane:methanol. The proposed method was compared with the traditional Roese-Gottlieb method for a series of milk samples with fat content ranging from < 1 to 37%. Fat determinations for a series of five milk types by the dry column and Roese-Gottlieb methods were significantly different for whole raw milk and buttermilk, while means for phospholipids were significantly different for all milk types tested except buttermilk.  相似文献   

3.
Milk lipids are an interesting source of bioactive molecules with functional and nutritional properties. Although the composition of milk lipids is of utmost importance for food processing and human consumption, it is far from being fully known. The objective of this study was to perform a comparative analysis of the chemical composition of lipids from bovine milks produced in French Brittany during spring (fresh pasture based diet) and winter (corn silage based diet). The polar lipid content and relative proportions of the glycerophospholipids and sphingomyelin were determined using HPLC/ELSD. The fatty acid composition of total lipids and polar lipids was determined using GC. The milks collected in spring contained i) a lower amount of total lipids: 39.7 ± 0.8 g/kg vs 41.7 ± 0.5 g/kg in winter, ii) a higher amount of polar lipids: 138 ± 11 vs 112 ± 8 mg/kg milk; 3.5 ± 0.3 vs 2.7 ± 0.4 mg/g fat, which was related to a smaller size of fat globules, and iii) a higher amount of sphingomyelin, 32 mg/kg milk vs 25 mg/kg milk in winter. Interestingly, the polar lipids from the milk fat globule membrane contained a higher concentration of unsaturated fatty acids in spring (C18:1 n  9, C18:2 n  6, C18:3 n  3 and long-chain n  3 fatty acids). Milk from cows fed a fresh pasture-based diet during spring is an interesting source of dietary long chain polyunsaturated fatty acids for human consumption.  相似文献   

4.
Buttermilk contains the milk fat globule membrane (MFGM), a material that possesses many complex lipids that function as nutritionally valuable molecules. Milk-derived sphingolipids and phospholipids affect numerous cell functions, including regulating growth and development, molecular transport systems, stress responses, cross membrane trafficking, and absorption processes. We developed a two-step method to produce buttermilk derivative ingredients containing increased concentrations of the polar MFGM lipids by microfiltration and supercritical fluid extraction (SFE). These processes offer environmentally benign alternatives to conventional lipid fractionation methods that rely on toxic solvents. Firstly, using a ceramic tubular membrane with 0.8-micron pore size, we evaluated the cross flow microfiltration system that maximally concentrated the polar MFGM lipids using a 2n factorial design; the experimental factors were buttermilk source (fresh, or reconstituted from powder) and temperature (50 degrees C, and 4 degrees C). Secondly, a SFE process using supercritical carbon dioxide removed exclusively nonpolar lipid material from the microfiltered buttermilk product. Lipid analysis showed that after SFE, the product contained a significantly reduced concentration of nonpolar lipids, and a significantly increased concentration of polar lipids derived from the MFGM. Particle size analysis revealed an impact of SFE on the product structure. The efficiency of the SFE system using the microfiltration-processed powder was compared much more favorably to using buttermilk powder.  相似文献   

5.
Oil-in-water pre-emulsions (15% sunflower + 5% olive oils) obtained by colloid mill homogenization (CM) at 5000 rpm using whey protein isolate at different levels (1, 2 and 4%) were stabilized by ultra high-pressure homogenization (UHPH, 100 and 200 MPa) and by conventional homogenization (CH, 15 MPa). Emulsions were characterized for their physical properties (droplet size distribution, microstructure, surface protein concentration, emulsifying stability against creaming and coalescence, and viscosity) and oxidative stability (hydroperoxide content and thiobarbituric acid reactive substances, TBARs) under light (2000 lux/m2 for 10 days). UHPH produced emulsions with lipid droplets of small size in the sub-micron range (100–200 nm) and low surface protein with unimodal distribution when produced at 4% whey proteins and 200 MPa. All emulsions exhibited Newtonian behavior (n  1). Long term physical stability against creaming and coalescence was observed in UHPH-emulsions, compared to those obtained by CM and CH. However, CH emulsions were highly stable against creaming (days) in comparison to the CM emulsions (hours). UHPH resulted in emulsions highly stable to oxidation compared to CM and CH treatments, especially when 100 MPa treatment was applied.Industrial relevanceIn the food, cosmetic and pharmaceutical sectors, industrial operators are currently interested in developing encapsulating systems to delivery bioactive compounds, which are generally hydrophobic, unstable and sensitive to light, temperature or/and oxygen. Ultra high-pressure homogenization is capable of producing stable submicron emulsions (< 1 μm) with a narrow size distribution, inducing more significant changes in the interfacial protein layer thus preventing droplet coalescence and also inhibit lipid oxidation. The present study suggests that emulsions produced by whey protein (4%) treated by ultra high-pressure homogenization have a good physical stability to flocculation, coalescence and creaming and also high stability to lipid oxidation, opening a wide range of opportunities in the formulation of emulsions containing bioactive components with lipid nature.  相似文献   

6.
The main objective of this work was to assess the technical and economical feasibility of proteins recovery from fish meal effluents using crossflow membrane technology, namely, ultrafiltration (UF) and nanofiltration (NF).Mackerel processing effluents were pre-treated by microfiltration (MF cartridges battery of 80, 20 and 5 μm pore size) followed by UF (membrane Carbosep M2, 15 kDa MWCO) or NF (membrane Kerasep NanoN01A, 1 kDa MWCO). A suitable treatment for the fish meal effluents consisted of a MF pre-treatment followed by UF operating at 4 bar, 4 m/s, natural pH and ambient temperature. UF yielded an average permeation flux of 28 l/(m2 h),1 and 62% proteins rejection for a volume reduction factor of 2.3. Both membranes fully recovered their original permeabilities through an acidic/basic washing cycle. The economic assessment of proteins recovery from fish meal effluents by UF was accomplished for a production of 544 ton/yr of fish meal (66% protein content), yielding a net present worth of 160×103 US$, interest rate of return of 17% and payback time of 8 years.  相似文献   

7.
Pilot scale production of a dairy ingredient enriched in phospholipids (PLs) was generated from a buttermilk powder (BMP) substrate utilising a combined process of targeted enzymatic hydrolysis of the innate milk proteins followed by ultrafiltration with a 50 kDa membrane. An 8.5 fold increase in PL was achieved in the 50 kDa retentate (50 R) compared to the BMP, 11.05 ± 0.02% and 1.30 ± 0.00% total PL, respectively. Simultaneously, total lipid content in the retentate increased 8.7 fold with reference to the BMP, 60.07 ± 0.54% and 6.84 ± 0.17% total lipid respectively. Protein reduced to 10.58 ± 0.09% (50 R) from 31.40 ± 0.57% in BMP. Supercritical CO2 fluid extraction (SFE) was employed to generate a purified lipid fraction. SFE with ethanol as a co-solvent yielded a purified lipid extract with enriched PLs level of 56.24 ± 0.07% on a dry matter basis.Industrial relevancePLs have many associated health and nutritional benefits including those related to cognitive development and repair. Generation of an ingredient enriched in dairy PLs would be advantageous from an industrial view to allow fortification of nutritionals, both infant and geriatric, in promoting brain health. The present work demonstrates a novel pilot scale process for the generation of a PL enriched ingredient from a BMP substrate. Utilising a combined process of targeted protein hydrolysis followed by selective removal by ultrafiltration of the smaller molecular weight peptide material, an ingredient with 8.5 fold increase in PL material was achieved. SFE technology was utilised to generate a purified lipid extract with greater PL levels for future applications in biological assays to determine these pathways. The need for investigate and further develop the knowledge relating to the modes of action of these bioactive compounds would be beneficial from a nutritional perspective.  相似文献   

8.
The effects of high pressure homogenization (HPH) on microbial inactivation and quality attributes (physio-chemical properties, bioactive components and antioxidant capacity) of mango juice, as well as their changes during storage at 4 °C and room temperature were investigated. Pressure levels ranged from 40 to 190 MPa, the inlet temperature from 20 °C to 60 °C and the number of passes from 1 to 5. Complete inactivation of molds and yeasts was achieved by 1 and 3 passes at 190 MPa and 60 °C, while total plate count was below 2.0 log10 CFU/mL. No multiplication of microorganisms was observed in mango juice over 60 days of storage at 4 °C. HPH treatment could retain or even increase the carotenoids and total phenols by 11.8% and 21.4%, respectively, while significant reductions were found for heat treatment (HT) samples. During the storage of 60 days, HPH treatment also provided better preservation of color, bioactive components and antioxidant capacity of mango juice than HT.Industrial relevanceHigh pressure homogenization (HPH) is a novel non-thermal technique, particularly suitable for continuous production of liquid foods limiting thermal damage and promoting “freshness”. Results showed that high pressure homogenization had the advantage of notably reducing the microbial load to levels equivalent to thermal pasteurization. Moreover, HPH treatment was superior to heat treatment with regard to post-treatment levels of bioactive.  相似文献   

9.
The relationships between textural and cooking qualities of Chinese noodles and flour lipids were investigated. Removal of free lipids (FL) or excess FL caused significant decreases in hardness and cohesiveness of noodles. The highest hardness and cohesiveness of noodles were obtained at certain FL levels (1.84 and 1.24 g/100 g flour, respectively). Tensile strength and breaking length of noodles both reached the highest values when FL content in the flour was adjusted to about half of the original level. A good correlation was found between cohesiveness and cooking loss of noodles (r = ?0.967). Hardness and cohesiveness of cooked noodles increased linearly with the increase of polar lipids (PL) relative proportion of FL (r = 0.939 and 0.900, respectively). Entire replacement of PL with nonpolar lipids in flour led to a significant decrease in springiness of noodles. These results suggest that PL play an important role in obtaining strong noodle texture.  相似文献   

10.
Myofibrillar proteins (MPs) of chicken breast were generally insoluble in water. The potential of high-pressure homogenization (HPH) to solubilize chicken breast MPs in water was tested. The effects of 0 psi (0.1 MPa), 10,000 psi (69 MPa), 15,000 psi (103 MPa) and 20,000 psi (138 MPa) for two passes HPH on solubility, protein profile, particle property, flow property and microstructure of MPs in water were investigated. HPH at 15,000 psi (103 MPa) could induce the suspension of MPs with small particle size species (sub-filament, oligomers or monomer structure) and high absolute zeta potential, thus enhancing the solubility, flow ability and stability without individual protein degradation. Reduction of particle size and strengthening of intermolecular electrostatic repulsion appeared to be the main reasons in solubilizing MPs in water treated with HPH.Industrial relevanceThe qualitative characteristics of meat products are closely related to the solubility of meat proteins. Myofibrillar proteins (MPs), as major part of total muscle proteins, are generally considered to be insoluble in water. The results showed that high-pressure homogenization has potential application for solubilizing MPs in water to develop new meat-based products in the food industry.  相似文献   

11.
Stabilization efficiency in terms of long term ambient temperature storage viability of the probiotic strain Lactobacillus casei CRL 431 was compared using freeze and fluidized bed drying techniques. Fluidized bed drying was able to retain 2.5 log cfu/g higher viability after 52 weeks of storage at 25 °C. A combination of fluidized bed drying and osmotic stress adaptation to the probiotic cells yielded further improvement of 0.83 log cfu/g higher viability compared to the unstressed cells. The findings were validated with other two lactobacilli and two bifidobacterium strains with probiotic characteristics and significant improvements in storage stability over freeze dried samples were observed. Fortification of vitamin E in the stabilization matrix as an antioxidant improved the stability by 0.18 log cfu/g during 20 weeks storage period at 25 °C, whereas any similar benefit of fortifying inulin as a prebiotic was not observed.  相似文献   

12.
The chemical, sensory and microbial qualities of candied-pineapple and cherry cakes were investigated. Both cake samples were also analyzed for chemical composition and microbial load before, during and after 56 days storage at room temperature. There were significant differences (p < 0.05) in the physico-chemical composition of the cherry cake and candied pineapple cake except for protein values of 9.00% and 8.63% respectively and magnesium content with values of 0.23% and 0.27% respectively. Sensory evaluation results showed significant differences (p < 0.05) between the two samples with the exception of colour (7.84) for both samples and texture (7.84 and 7.80) for cherry and candied pineapple cakes, respectively. In terms of general acceptability, the candied-pineapple cake was most preferred (8.52). The microbial loads (bacterial and fungal) of the candied pineapple cake were lower than the cherry cake throughout the duration of the 56 days storage at room temperature. The highest bacterial load was recorded after the 28th day (148.0 × 105 cfu/g) and (194.30 × 105 cfu/g) for candied pineapple and cherry cakes respectively. The fungal load was also highest at 28th day of storage (14.33 × 101 cfu/g) and (12.67 × 103 cfu/g) for candied pineapple and cherry cakes respectively. The total viable counts (TVC) were within acceptable limits for human consumption. Therefore, candied-pineapple can readily substitute cherry in fruit cake making in Nigeria.  相似文献   

13.
《Meat science》2013,93(4):837-840
This study aimed to measure the amount of microbial contamination caused by inspecting the lymph nodes of adult sheep carcasses for caseous lymphadenitis (CLA). Surface swabs from carcasses pre-inspection (N = 296) and post-inspection (N = 296) were obtained for enumeration of indicator organisms at three commercial abattoirs. At the scapular site, inspection doubled the probability of detecting E. coli (Pr before = 0.35, Pr after = 0.67) and increased the expected count of E. coli from 2 cfu/cm2 to 13 cfu/cm2. Inspection at the rump site increased the probability of detecting E. coli by 1.1 times (Pr before = 0.84, Pr after = 0.93) and increased the expected count from 32 cfu/cm2 to 45 cfu/cm2. Effects were also observed for Enterobacteriaceae and total viable count. The findings show that routine inspection of adult sheep carcasses for CLA has a detrimental impact on carcass microbiological traits.  相似文献   

14.
Total lipids of olive pomace (OP), olive pomace diet (OP diet), fish oil diet (FO diet) and fish filets of farmed gilthead sea bream (fish fed with FO diet and OP diet respectively) were extracted and separated into polar (TPL) and neutral (TNL) lipids. All samples were assessed for their in vitro activity against washed rabbit platelets aggregation induced by platelet activating factor (PAF) and they were further analyzed by electrospray-mass spectrometry. The high levels of palmitic (16:0), oleic (18:1 cis ω  9), linoleic (18:2 ω  6) and docosapentaenoic acid (DPA 22:5 ω  3) contained in both OP and FO diets are reflected to the gilthead sea breams fed with the individual diet respectively, while the gilthead sea bream fed with FO diet displays a decrease in DPA. All samples contained various glycerophospholipids species. Two PE species were identified in OP, OP diet and fish fed with OP diet and not in FO diet, while that might be an indication that these substances are likely to be the key polar phospholipids that have the ability to be in vitro PAF inhibitors, i.e. inhibit the formation of atherosclerotic plaques in blood arteries.  相似文献   

15.
A combination of mechanical tissue disintegration techniques (i.e. blending and high pressure homogenization) and heat treatments (i.e. high and low temperature) was deliberately applied in processing carrot purées. The chemical structure of serum pectin and its influence on the consistency of the differently prepared purées were evaluated. High temperature treatment of carrot pieces prior to high pressure homogenization (HTT + HPH) resulted in high apparent molar mass (MM) serum pectin, while the reverse order of purée preparation (HPH + HTT) generated a relatively lower MM. The exceptional high apparent MM of HTT + HPH sample is possibly related to proteins bound to pectin. The importance of the order of heat treatment and tissue disruption was also reflected in largely different carrot purée consistencies in which HTT + HPH was more consistent. Low temperature treated (LTT) carrot purées, whereby endogenous pectin methyl-esterase was stimulated, had less consistent purées and low molar mass serum pectins.Industrial relevanceIntense high pressure homogenization enhances the solubilization of pectin in the serum fraction of plant-based dispersions (e.g. purées, soups, juices and sauces). This offers the potential of tailoring the concentration and structure of serum pectin that contributes to the functionality (e.g. consistency, homogeneity) of plant-based dispersions. It was shown that the order of thermal and mechanical unit operations results in different serum pectin molar mass distributions as well as carrot purée consistencies. This further enables the production of more natural plant-based food products with targeted functionalities.  相似文献   

16.
This paper describes a two-stage process for separating milk proteins from pasteurized skim milk in three fractions: casein micelles, β-Lactoglobulin (β-Lg) and other large whey proteins, and α-Lactalbumin (α-La). Casein micelles were extracted in the retentate of a microfiltration using rotating ceramic disk membranes. α-La and β-Lg transmissions remained between 0.8 and 0.98. Their yields in permeate reached 81% for α-La and 76.6% for β-Lg at a VRR of 5.4. The separation between β-Lg and α-La was carried out by UF using a rotating disk module equipped with a 50 kDa PES circular membrane. Permeate fluxes were very high, remaining above 340 L h?1 m?2 at VRR = 5 and 40 °C. α-La transmission remained generally between 0.2 and 0.13 giving yields from 28% to 34%. β-Lg rejection was above 0.94, giving a maximum selectivity of 4.2. These data confirm the potential of dynamic membrane filtration for separating α-La and β-Lg proteins from skim milk.  相似文献   

17.
《Meat science》2013,93(4):823-828
The effect of high pressure (HP) on Salmonella enterica subsp. enterica serovar Enteritidis in beef carpaccio stored under temperature abuse conditions (8 °C) during 30 days was investigated. After treatment, reductions of S. Enteritidis were 3.68 and 5.94 log cfu/g in samples pressurized at 450 MPa for 5 and 10 min, respectively, whereas the pathogen was only detected after enrichment of samples treated at 450 MPa for 15 min. During storage, counts of S. Enteritidis decreased 0.26 log cfu/g in non-pressurized carpaccio, 1.33 log cfu/g in carpaccio treated at 450 MPa for 5 min and were only detected after enrichment in carpaccio pressurized at 450 MPa for 10 or 15 min. Color (L*, a* and b*) varied with pressurization and storage, with higher changes in carpaccio treated at longer pressurization times. Shear resistance was slightly lower in treated samples just after pressurization, but increased at the end of the storage period. Maximum force was less affected by treatment.  相似文献   

18.
Electrohydrodynamic (EHD) drying is considered as energy efficient non-thermal technology suitable for dewatering of heat-sensitive materials, including food products. A factorial experimental design was used to identify significant factors affecting mass transfer in EHD drying. The experiment revealed significant effects of voltage, distance (gap) between electrodes, configuration of discharge electrode, air cross-flow and the characteristics of material surface on mass transfer. Strong coupling between mass and charge transfer was found for all voltages, gaps and configurations of discharge electrode. The effect of air cross-flow at 1.0 m/s on mass transfer was additive to the effect of ionic wind, consistently increasing total mass transfer by 5.0–5.1 g/h in all experimental conditions. These results led to the conclusion that the effect of EHD is convective in nature, enhancing mass transfer due to ionic wind. The effect of material surface characteristics was attributed to different hydrodynamic conditions of air boundary layer.Industrial relevanceThis research is focused on better understanding of the factors that play a significant role in EHD drying, and therefore it is important for industry to facilitate practical applications of EHD in bioprocessing and food engineering.  相似文献   

19.
The effect of processing conditions on the formation and stability of a gel comprised of oil, water, monostearin and stearic acid was studied. Processing conditions (homogenization rate (10,000–30,000 rpm), cooling rate (0.05–20 °C/min), homogenization temperature (70–95 °C)) had no effect on the initial storage modulus and melting temperature of the gel. Salt (NaCl) addition to the aqueous phase in the range 0–0.25% did not affect these parameters either; however, 0.5% salt addition lead to a ∼2 °C decrease in melting temperature and a doubling of the storage modulus. Optimal preparation conditions for the gel were mainly determined from its microstructure by polarized light microscopy – a smaller globule size and a more homogenous size distribution were considered a desirable feature. Optimum conditions included homogenization rates greater than 20,000 rpm, cooling rates above 6 °C/min, homogenization temperatures above, but close to, the Krafft temperature of monostearin (72 °C), and 0% salt concentration. The gelation temperature decreased by 2 °C with each 1 °C/min increase in cooling rate, from 1 °C/min to 5 °C/min.  相似文献   

20.
In this study, the applicability of semi-direct cold atmospheric pressure plasma (CAPP) during postharvest processing of Tenebrio molitor flour is investigated. Besides analyzing the decontamination efficacy, plasma-induced impact on techno-functionality, protein solubility, composition and structure was determined and compared to heat induced effects.Following CAPP treatment, the total microbial load of the Tenebrio flour of 7.72 log10 cfu/g was reduced to 7.10 (1 min), 6.72 (2.5 min), 5.79 (5 min), 5.19 (7.5 min), 5.21 (10 min) and 4.73 (15 min) log10 cfu/g. With increasing exposure to CAPP, protein solubility at pH 4 almost linearly decreased to a minimum of 54%. Water binding capacity decreased from 0.79 to 0.64 gwater/g whereas oil binding capacity increased from 0.59 to 0.66 goil/g. Gel electrophoresis revealed a decrease of all protein fractions at pH 4 whereas at pH 10 the band pattern significantly shifted to protein fractions with higher molecular weights.Industrial relevanceEdible insects are rich in valuable protein, fat, fibre, minerals and micronutrients. Although a wide range of species represent a valuable alternative protein source that could contribute to food and feed security, they are industrially hardly exploited. The tailored application of proper processing technologies could lead to novel insect-based high-protein food and feed products with unique functional properties supporting the increase in acceptability among potential consumers. Current research concentrates on developing processing chains including innovative nonthermal approaches. Cold atmospheric pressure plasma (CAPP) has gained attention as an effective technology for the decontamination and modification of fresh and dry agricultural products. In the postharvest chain of edible insects, the application of CAPP could contribute to the development of safe and high-quality insect-based products in the food and feed sector.  相似文献   

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