蕨麻对面包制品品质特性的影响

主要研究面粉中添加1％～9％的蕨麻粉对面团粉质特性以及面包品质的影响。结果表明．随着蕨麻粉添加量的增加,面团的吸水率逐渐增高,面团的形成时间和稳定时间延长,弱化度降低;随着蕨麻粉添加比例的增加,面包硬度增加,面包的感官评分总体上呈现降低趋势,但是当蕨麻添加量大于5％时,面包体积急剧减少,面包纹理结构变差,质地和口感都明显下降,因此,蕨麻粉在面包粉中的合理添加比例为5％。     

Effect of soybean addition on the rheological properties and breadmaking quality of wheat flour

The effects of enzyme‐active full‐fat (EAFFSF), heat‐treated full‐fat (HTFFSF) and enzyme‐active defatted (EADSF) soy flours and commercial soy protein isolates (SPIs) on mixing properties and extensibility of dough, gluten formation, gas production and retention properties of dough and bread quality were studied. The soy products utilised in this study presented values of urease activity, nitrogen solubility index (NSI) and enthalpies of protein denaturation according to their previous heat processing. Soy products interfered in gluten formation, weakened dough strength and decreased dough gas retention capacity. Bread quality was negatively affected by soy product addition. The negative effects were exacerbated by an increase in soy/wheat ratio. Soybean protein state was identified as an important factor in determining dough and bread properties. Copyright © 2005 Society of Chemical Industry     

Physico-chemical properties and rheological behaviour ofPatella caerula paramyosin

Paramyosin is a muscle protein which is characteristic of all invertebrates but which is not present in vertebrate muscles. Given the functional importance of paramyosin, the purpose of this paper was to study the physico-chemical properties, including the amino acid composition and rheological behaviour, of purified paramyosin and to investigate its mode of interaction with myosin. Paramyosin was purified from the limpet (Patella caerula) by an ethanol precipitation step. It was soluble at ionic strengths below 0.05 m NaCl and its maximum solubility at neutral pH occurred at approximately 0.4 M NaCl. At this high ionic strength, the pH dependence of solubility was such that paramyosin passed quickly into solution when pH exceeded pH 5, the transitional pH value. By using an immunological method, it was shown that interactions between paramyosin and myosin occurred, even in the presence of actin. The molecular assembly of both proteins was probably specified by hydrophobic interactions, as well as by interactions enhanced by divalent cations. The changes in the dynamic shear storage modulus (G′) started between 40°C and 50°C, and reached a maximum at about 75°C.     

Wheat protein composition and properties of wheat glutenin in relation to breadmaking functionality

The unique breadmaking properties of wheat are generally ascribed to the visco-elastic properties of its gluten proteins. While monomeric gluten proteins (gliadin) show viscous behavior, polymeric gluten proteins (glutenin) are elastic. The unique elasticity of glutenin results to a large extent from its polymeric nature. Glutenin is a highly heterogeneous mixture of polymers consisting of a number of different high- and low-molecular-weight glutenin subunits linked by disulfide bonds. Although glutenin obviously is the major polymeric protein in wheat, other polymeric proteins occur as well. Their importance in breadmaking may be underestimated. Nevertheless, variations in both quantity and quality of glutenin strongly determine variations in breadmaking performance. Structural features of different classes of glutenin subunits are described. Variations in glutenin quality may result from variations in its (1) structure, (2) size distribution, and (3) subunit composition. Some hypotheses on glutenin structure and current insights into the role of glutenin size distribution are evaluated. Finally, different ways in which variation in glutenin composition may directly or indirectly (by affecting glutenin structure and/or size distribution) influence glutenin quality are discussed.     

Effect of grain hardness,fractionation and cultivars on protein,pasting and dough rheological properties of different wheat flours

Coarse flour fractions (CFFs) and fine flour fractions (FFFs) obtained from flour milled from twelve different wheat cultivars varying in grain hardness index (GHI) were evaluated for particle size distribution, pasting and protein characteristics. Cultivars with greater hardness produce flour with high protein content had more proportion of large size particles. FFF had higher unextractable polymeric protein, solvent retention capacity (SRC), sedimentation value (SV) and dough stability (DS) than their corresponding CFF. Both FFF and CFF from cultivars with lower hardness showed lower sodium SRC. CFF showed higher pasting viscosities than their corresponding FFF, and difference in these properties was greater amongst soft cultivars. DS increased with decrease in grain hardness, but medium hard cultivars showed exceptionally higher value. The concentration of HMW‐GS in the CFF was higher than FFF of hard wheat cultivars, whereas the concentration of LMW‐GS in the CFF and FFF was not influenced by the fractionation of flour.     

Fatty acid composition and rheological behaviour of prickly pear seed oils

Prickly pear fruits constituted valuable foodstuff for humans and animals in arid and semi-arid regions. Two species of prickly pear from Tunisia, Opuntia ficus indica and Opuntia stricta, were investigated for fatty acid composition and physicochemical parameters of the seed oil. No significant difference in either physical or chemical parameters was found between the species. The main fatty acids of prickly pear seed oil were C16:0, C18:0, C18:1, C18:2. With an exceptional level of linoleic acid, up to 70%, the content of unsaturated fatty acids was high, at 88.5% and 88.0% for O. ficus indica and O. stricta, respectively.Rheological properties were analysed with changes of temperature and shear stress. Variations of viscosity were measured and the viscoelastic parameters were determined during heating and cooling cycles between 20 and 70 °C. Curves of flow were established with up and down cycles of shear stress at different temperatures. These measures highlighted the presence of large aggregates of crystal fatty acids in both Opuntia crude oils. Shearing and temperature destroyed this structural state and gave birth to an homogeneous stable suspension.The structural state of crude oil was confirmed using a contrast phase microscope, and the particle size distribution was obtained by laser granulometry.     

Enzyme activities,dietary fibre components and rheological properties of wholemeal flours from rye cultivars grown in Finland

Total and soluble dietary fibre, total and soluble pentosan and β‐glucan contents, activities of α‐amylase (EC 3.2.1.1) and endo‐β‐xylanase (EC 3.2.1.8) and viscous properties of aqueous suspensions of wholemeal flours during heating were determined in nine winter rye cultivars (Secale cereale L) grown in Finland in 1998–2001. There was marked annual and varietal differences in grain quality. In the rainy summer 1998 the yield was low, grains were small and dietary fibre content of the grains was high. Xylanase activity of the grains was high, which corresponded to the high content of soluble pentosans. In the dry summer of 1999, the pentosan content of the grains was low and β‐glucan content high. The effect of weather conditions and cultivar were also apparent in the differences in falling numbers, amylogram and swelling curve results. The two hybrid rye cultivars Esprit and Picasso had consistently highest falling numbers and amylogram peak viscosities. The activities of α‐amylase and xylanase had a moderate positive correlation with total pentosan content and the content of soluble pentosan. Xylanase activity had better correlation with the viscous properties of flour–water suspensions than α‐amylase. Surprisingly, α‐amylase activity had only a moderate negative correlation with falling number. Copyright © 2005 Society of Chemical Industry     

Effect of cereal,fungal and bacterial α-amylase on the rheological and breadmaking properties of medium-protein wheats

Optimal levels of cereal malt, fungal and bacterial α-amylase for baking of medium-protein flours were found to be 5 - 10. 20 and 0.35 SKB/100 g, respectively. The dough consistency decreased considerably with time, depending on the source and level of α-amylase. The paste viscosities of the flours were reduced considerably by the cereal malts even at 2.5 SKB/100 g than by the fungal and bacterial α-amylase used optimally. Satisfactory loaf volumes were obtained with optimised α-amylase supplements without extraneous sugar in the bread formula. With 0.35 SKB of bacterial α-amylase, a bread having distinguishably softer texture was obtained as compared to cereal and fungal α-amylases. Accumulated maltose in the crumb indicated the need for a strain of baker's yeast with an active maltase system to render in situ contribution of α-amylase supplements more effective.     

Some properties of corn grains and their flours I: Physicochemical,functional and chapati-making properties of flours

The variability in physical (1000 kernel weight and bulk density) and mechanical (rupture force) properties of grains from different Indian corn varieties (African tall, Ageti, Early composite, Girja, Navjot, Parbhat, Partap, Pb sathi and Vijay) were studied. The functional (colour, gelatinization, retrogradation and pasting) and chapati-making properties of flours milled from corn varieties were evaluated. African tall flour showed the highest enthalpy of gelatinization (ΔH_gel), peak-, trough-, breakdown-, final-, and setback viscosities, and L^∗ (84.4) value and resulted in chapaties with higher extensibility (5.76 mm) and of light colour. African tall flour, with the lowest protein content, showed the lowest grain rupture force. Amylose content and hardness of starch gel from African tall were found to be the lowest among all corn varieties. Girja flour, with the lowest transition temperatures and ΔH_gel, showed the lowest extensibility of chapaties made from it. Pearson correlations between physical and textural properties of corn grains and the functional properties of their flours were established. Rupture force of corn grain and protein content of flour showed a negative correlation with peak viscosity of flour (r = −0.917, and −0.863, p < 0.01). The protein content of flours was negatively correlated with L^∗ (r = −0.759, p < 0.01) value and positively with b^∗ (r = 0.635, p < 0.01) value. Pasting temperature of flours showed a significant negative correlation with peak, trough, breakdown, final and setback viscosities (r = −0.836, −0.846, −0.778, −0.871, and −0.847, respectively, p < 0.01). Pearson correlation was also established between the grain and starch properties. Rupture force of corn grains was positively correlated with the amylose content of starch (r = 0.950, p < 0.01).     

Physico-chemical properties and rheological behaviour ofPatella caerula paramyosin

Paramyosin is a muscle protein which is characteristic of all invertebrates but which is not present in vertebrate muscles. Given the functional importance of paramyosin, the purpose of this paper was to study the physico-chemical properties, including the amino acid composition and rheological behaviour, of purified paramyosin and to investigate its mode of interaction with myosin. Paramyosin was purified from the limpet (Patella caerula) by an ethanol precipitation step. It was soluble at ionic strengths below 0.05 m NaCl and its maximum solubility at neutral pH occurred at approximately 0.4 M NaCl. At this high ionic strength, the pH dependence of solubility was such that paramyosin passed quickly into solution when pH exceeded pH 5, the transitional pH value. By using an immunological method, it was shown that interactions between paramyosin and myosin occurred, even in the presence of actin. The molecular assembly of both proteins was probably specified by hydrophobic interactions, as well as by interactions enhanced by divalent cations. The changes in the dynamic shear storage modulus (G) started between 40°C and 50°C, and reached a maximum at about 75°C.     

Fatty acid composition,oxidative stability,antioxidant and antiproliferative properties of selected cold-pressed grape seed oils and flours

Cold-pressed chardonnay, muscadine, ruby red, and concord grape seed oils and their defatted flours were studied for their fatty acid composition, oxidative stability and antioxidant and antiproliferative activities. The phenolic profiles of the seed flours were also measured. The most abundant fatty acid in the oils was linoleic acid, ranging from 66.0 g/100 g of total fatty acids in ruby red seed oil to 75.3 g/100 g of total fatty acids in concord seed oil. The oils were also high in oleic acid and low in saturated fat. Ruby red grape seed oil recorded the highest oxidative stability index of 40 h under the accelerated conditions. Total phenolic content (TPC) was up to 100 times lower in the oils than in the flours. Lutein, zeaxanthin, cryptoxanthin, β-carotene, and α-tocopherol levels were also measured. DPPH radical-scavenging capacity ranged from 0.07 to 2.22 mmol trolox equivalents (TE)/g of oil and 11.8 to 15.0 mmol TE/g of flour. Oxidative stability of menhaden fish oil containing extracts of the seed flours was extended by up to 137%. HPLC analysis was conducted to determine the levels of free soluble, soluble conjugated and insoluble bound phenolics in the seed flours. The phenolic compounds analyzed included catechin, epicatechin, epicatechin gallate, quercetin, gallic acid, and procyanidins B1 and B2. Antiproliferative activity was tested against HT-29 colon cancer cells. All of the seed flours and muscadine seed oil registered significant (P < 0.05) inhibition of cancer cell growth. The results from this study demonstrate the potential of developing value-added uses for these seed oils and flours as dietary sources of natural antioxidants and antiproliferative agents for optimal health.     

Effects of oxidase and protease treatments on the breadmaking functionality of a range of gluten-free flours

In this study, the application of glucose oxidase and protease commercial preparations was investigated in order to evaluate their impact on the breadmaking performance of four different gluten-free flours (buckwheat, corn, sorghum and teff). Bread formulas were developed without addition of hydrocolloids in order to avoid synergistic effects. Glucose oxidase improved corn (CR) and sorghum (SG) bread quality by increasing specific volume (P < 0.05) and reducing collapsing at the top. The improvements could be related to protein polymerization which resulted in enhanced continuity of the protein phase and elastic-like behavior of CR and SG batters. No significant effects were detected on buckwheat (BW) and teff breads. On the other hand, protease treatment had detrimental effects on the textural quality of BW and SG breads. The effects were related to protein degradation resulting in increased liquid-like behaviour of BW and SG batters. Overall, the results of this study suggest that protein polymerisation can improve the breadmaking performance of gluten-free flours by enhancing elastic-like behaviour of batters. However, the protein source is a key element determining the impact of the enzymes. In the absence of hydrocolloids, protein structures are important to ensure the textural quality of these types of breads.     

Gelation behaviour and rheological properties of acid-induced soy protein-stabilized emulsion gels

The protein concentration dependence on the rheological properties of acid-induced gels formed with unheated and heated soy protein-stabilized emulsions (UHSPE and HSPE) was investigated at different acidification temperatures. Pre-heat treatment on soy protein solutions resulted in a higher storage modulus (G′) and a shorter gelation time (t_gel) of acid-induced emulsion gels. A maximum in tan δ was observed in the UHSPE gels but no maximum was detected in the HSPE gels. Increasing the acidification temperature decreased the G′ and t_gel. The dependence of the G′ on the protein concentration (c) can be scaled with a power law: G′ ∼ c^A. The exponent (A) increased with pre-heat treatment and acidification temperature. The experimental data.fitted the fractal scaling model (G′ ∼ φ^A) and the simple time- scaling model above very well for the acid-induced soy protein-stabilized HSPE gels with varying oil volume fraction. The large deformation and fracture properties were significantly affected by soy protein concentration, pre-heat treatment, acidification temperature and volume fraction of oil droplets (p < 0.05).     

Influence of breadmaking on antioxidant capacity of gluten free breads based on rice and buckwheat flours

In the present study new formulations for gluten-free bread based on mixtures of rice (RF) and buckwheat flour (light buckwheat flour (LBF) or wholegrain flour (WBF)), in the proportions of 90:10, 80:20, and 70:30 were made. The gluten-free breads were investigated for their total phenolic content, rutin and quercetin contents, antioxidant activity (AOA) by β-carotene bleaching method, reducing power, scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and chelating activity on Fe²⁺. The increased amount of LBF or WBF in the dough formulation resulted in the final products with higher antioxidant properties. Baking treatment expressed different influences on antioxidative properties of the final gluten-free product in terms of raw materials, applied recipe and antioxidative capacity in comparison to calculated values based on raw materials. Final gluten-free products were characterized by lower total phenolic and rutin contents, lower antioxidative and reducing activity and on the other hand higher DPPH and chelating activity as well as quercetin content in comparison to calculated values. Bread containing wholegrain buckwheat flour expressed in most of the cases higher values of measured antioxidative parameters than bread prepared with light buckwheat flour and thus contributes to their additional functional property.     

Lactobacillus Gasseri LGZ 1029 in yogurt: rheological behaviour and volatile compound composition

Yogurt preserves and enhances nutritional value of milk. In this study, we have compared several strains to determine the physicochemical, sensory, rheological and aroma characteristics of different yogurts. We used Lactobacillus gasseri LGZ 1029 (LG), commercial probiotic L. rhamnosus (LGG) and traditional fermentation strains Streptococcus thermophilus and L. bulgaricus (SL). Results showed that the flavour and texture characteristics of mixed-strain yogurts were obviously better than in single-strain yogurts. Addition of LG increased pseudoplastic behaviour, as shown by Herschel–Bulkley model analysis of rheological behaviour. The LG + SL group also had both the highest viscosity consistency index and thickening ability. In addition, a total of 57 volatile compounds were detected in yogurts and the fermentation with the addition of LG was mainly affected by ketones. Our study suggested that a yogurt with new attributes can be produced by using LGZ 1029.     

Correlation of fatty acid composition of vegetable oils with rheological behaviour and oil uptake

The fatty acid compositions of seven edible vegetable oils were investigated and correlated with their rheological behaviours and the amount of absorbed oils to fried products. All oil samples showed constant viscosity as a function of shear rate, exhibiting Newtonian behaviours. The highest viscosity was observed in hazelnut oil, followed by olive, canola, corn, soybean, sunflower, and grapeseed oils. In addition, a high correlation (R² = 0.94) demonstrated that the flow behaviours of vegetable oils were positively governed by their major components (18:1 and 18:2 fatty acids). It was also shown that a more rapid change in viscosity with temperature was observed in the oils containing more double bonds (R² = 0.71). Furthermore, even though the overall tendency was that the potato strips fried in the oils with high viscosity appeared to cause more oil uptake, a significant effect of oil types on oil uptake was not observed.     

电子束辐照对小麦分离淀粉流变学特性的影响

从经过电子束辐照处理的小麦中提取淀粉,用Brabender连续粘度计研究不同辐照剂量（0、1.0、2.75、4.4kGy）对其流变学特性的影响,并用排阻液相色谱和多角度光散射仪、折光检测器连用技术（HPSEC-MALLS-RI）测定其分子量分布。结果表明：随着辐照剂量的增加,小麦淀粉的Brabender峰值黏度显著降低,冷稳定性升高,凝沉性减弱,当辐照剂量达到4.4kGy时,小麦淀粉的峰值黏度由85.0BU减小到42.0BU,下降了50.6%,回生值下降了88.2%;同时,小麦淀粉重均分子量逐渐下降,且当辐照剂量达到4.4kGy时,小麦淀粉的重均分子量与未辐照相比下降了1个数量级,说明电子束辐照引起了小麦中淀粉分子的降解,平均分子量减小,导致小麦淀粉流变学特性改变,粘度降低。