HPLC法测定粉萆薢和绵萆薢中薯蓣皂苷元的含量

建立了高效液相色谱法对粉萆薢和绵萆薢药材中薯蓣皂苷元的含量测定方法,通过测定比较两者含量大小。方法:采用反相高效液相色谱法:色谱柱为Gemini C18(250 mm×4.6 mm,5μm),甲醇∶水(95∶5)为流动相,流速:1.0 mL/min,检测波长:210 nm,对粉、绵萆薢药材中的薯蓣皂苷元进行含量测定。结果表明:线性范围为0.120 5~1.205 mg/mL,r=0.9997,平均回收率99.59%,RSD=0.46%(n=5),粉萆薢的平均含量为0.481 1 mg/mL,绵萆薢的平均含量为0.101 0 mg/mL。本实验方法简便,快速,结果可靠,可用于对粉、绵萆薢药材中薯蓣皂苷元的含量测定,得出粉萆薢中薯蓣皂苷元的含量远大于绵萆薢中的含量。     

高效液相色谱法测定金刚藤口服液中薯蓣皂苷元的含量

建立高效液相色谱法测定金刚藤口服液中薯蓣皂苷元的含量。色谱条件:色谱柱为Kromasil C18 150×4.6 mm 5μm;Kromasil C18200×4.6 mm 5μm;Symmetryshield C18 150×3.9 mm 5μm;流动相为乙腈-水(90∶10);流速1.0 mL/min;检测波长205 nm;柱温35℃;进样量10μL、20μL。线性范围为0.55~5.5 mg/mL(r=0.9999),加标平均回收率为96.31%,RSD为2.06%。本方法准确度高、精密度高、重复性好、简捷易操作,可以作为金刚藤口服液中薯蓣皂苷元含量的测定方法。     

复合酶协同超声提取葫芦巴中薯蓣皂苷的工艺研究

研究复合酶协同超声提取葫芦巴中薯蓣皂苷的工艺。以葫芦巴中薯蓣皂苷得率为考察指标,通过单因素试验及L_9(3~4)正交试验,探讨纤维素酶、果胶酶复合酶制剂的使用量、酶解时间、酶解温度、pH值对薯蓣皂苷得率的影响,优化复合酶协同超声提取葫芦巴中薯蓣皂苷的工艺条件。结果表明,最佳酶解条件为复合酶制剂用量为2.5%,酶解温度为30℃,pH值为6.0,酶解时间为2h。在此条件下,葫芦巴中薯蓣皂苷的得率为2.29%,经比较,复合酶协同超声提取薯蓣皂苷的得率比直接超声提取增加了36.3%。     

HPLC-ELSD同时测定知母药材中3种主要皂苷含量

目的:首次建立同时测定知母药材中3种主要皂苷含量的HPLC-ELSD法。方法:采用Diamonsil C18(2)柱(250×4.6mm,5um),流动相为甲醇(A)-水(B),梯度洗脱;流速1.0m L·min~(-1);柱温30℃;蒸发光散射检测器雾化器流速2.0L·min~(-1);漂移管温度50℃;进样量10u L。结果:知母皂苷O、知母皂苷BII、知母皂苷BI浓度分别在64.13~1026μg·mL~(-1)(r=0.9989)、57.15~1028μg·mL~(-1)(r=0.9965)、67.32~1077μg·mL~(-1)(r=0.9998)的范围内呈良好的线性关系。加样回收率平均值为99.43%~102.8%,RSD为1.78%~2.58%。结论:该含量测定方法简便,准确,可靠,能同时测定知母皂苷O、知母皂苷BII、知母皂苷BI 3种有效成分含量。     

HPLC法测定粉萆薜和绵萆薜中薯蓣皂苷元的含量

建立了高效液相色谱法对粉革薜和绵萆薜药材中薯蓣皂苷元的含量测定方法,通过测定比较两者含量大小。方法：采用反相高效液相色谱法：色谱柱为Gemini C18（250mm×4．6mm,5μm）,甲醇：水（95：5）为流动相,流速：1．0ml Mmin,检测波长：210nm,对粉、绵萆薜药材中的薯蓣皂苷元进行含量测定。结果表明：线性范围为0．1205—1．205mg／mL,r=0．9997,平均回收率99．59％,RSD=0．46％（n=5）,粉革薜的平均含量为0．4811mg／mL,绵革薜的平均含量为0．1010mg／mL。本实验方法简便,快速,结果可靠,可用于对粉、绵萆薜药材中薯蓣皂苷元的含量测定,得出粉革薜中薯蓣皂苷元的含量远大于绵革薜中的含量。     

HPLC法测定红参中人参皂苷Rg1的含量

建立红参药材中人参皂苷Rg1的含量测定方法。利用高效液相色谱(HPLC)法测定红参中人参皂苷Rg1的含量,采用Ulitimate XB-C18(2)柱(250 mm×4.6 mm,5μm);流动相∶乙腈-水(23∶77);柱温:室温;流速:0.7 m L·min-1;检测波长:203 nm;进样量为10μL。人参皂苷Rg1的线性回归方程为A=438355.8C-1328.9(r=0.9999)。人参皂苷Rg1在0.1~5μg范围内呈良好的线性关系。人参皂苷Rg1的平均回收率为103.6%,RSD为1.581%。采用此法测定红参中人参皂苷Rg1的含量,准确可靠,可用于该药材的质量控制。     

HPLC法测定复方扶芳藤合剂中人参皂苷Rb1的含量

建立复方扶芳藤合剂中人参皂苷Rb1的含量测定方法。利用高效液相色谱法,采用Ulitimate XB-C18(2)柱(250 mm×4.6 mm,5μm);流动相:V(乙腈)-V(水)(30∶70);柱温:室温;流速:0.7 m L·min-1;检测波长:203 nm;进样量:10μL。人参皂苷Rb1的线性回归方程为Y=816 921.4 X-689.2,r=0.999 9。人参皂苷Rb1在0.01~1μg范围内呈良好的线性关系。人参皂苷Rb1的平均回收率为99.08%,RSD为1.33%(n=6)。该方法稳定、重复性好,结果准确,可作为复方扶芳藤合剂中人参皂苷Rb1的含量测定方法。     

RP-HPLC-UV法测定清心莲子饮中人参皂苷Re、Rg1、Rb1的含量

目的采用RP-HPLC-UV色谱法同时测定清心莲子饮中人参皂苷Re、Rg_1、Rb_1的含量影响。方法采用色谱柱:依利特C_(18)柱(Sinochrom ODS-BP,4.6mm×200mm,5μm),流动相采用A为乙腈,B为水,梯度洗脱,流速为1.0mL·min~(-1),进样量为10μL,检测波长为203nm,柱温25℃,理论塔板数大于6000。结果在100min内,人参皂苷Re、Rg_1、Rb_1分离度良好,进样的含量与色谱峰面积具有良好的线性关系,人参皂苷Re、Rg_1、Rb_1的线性方程分别为:y=0.5752x-0.0961 (r=0.9994)、y=1.3157x-0.2006(r=0.9998)、y=0.6637x+0.2091(r=0.9997),人参皂苷Re、Rg_1、Rb_1的平均加样回收率分别是:101.08%(RSD=1.77%)、99.40%(RSD=2.55%)、100.11%(RSD=2.83%)。结论本文建立的含量测定方法快捷稳定可靠,加样回收率高,适用于清心莲子饮中人参皂苷Re、Rg_1、Rb_1的含量测定,为清心莲子饮的质量评价提供了理论支持和实验依据。     

高效液相色谱法定量分析竹节参中人参皂苷Rb1的含量

用RP-HPLC测定竹节参中人参皂苷Rb1的含量,采用Venusil ABS C18色谱柱(250 mm×4.6 mm5,μm),以乙腈-水(35∶65)为流动相,流速1 mL/min,检测波长203 nm。结果表明,人参皂苷Rb1在25～500μg/mL间线性关系良好(r=0.999 4),平均加样回收率为99.64%,RSD=1.52%。该方法快速、准确、重现性好,可用于竹节参中人参皂苷Rb1的含量测定。     

高效液相色谱法同时测定三七提取物中三七皂苷R1与人参皂苷Rg1、Rb1含量

建立了高效液相色谱法同时测定三七提取物中三七皂苷R1与人参皂苷Rg1、Rb1含量的方法。采用高效液相色谱仪,Agilent ODS-C18(5μm,4.6mm×250mm)液相色谱柱,流动相为乙腈-水梯度洗脱,流速为1.0 mL/mim,检测波长为203 nm,柱温为35℃。结果表明,三七皂苷R1在度15.29~244.60 mg/L的范围内(r=0.9998),人参皂苷Rg1在14.30~228.90 mg/L范围内(r=1.0000),人参皂苷Rb1在13.13~210.11 mg/L范围内(r=1.0000),线性关系良好。三七皂苷R1和人参皂苷Rg1、人参皂苷Rb1平均加标回收率分别为99.54%、101.61%、102.99%,峰面积相对标准偏差RSD(n=6)分别为1.67%、0.93%、0.92%。该方法简便、准确,重现性好,可以对三七提取物中三七皂苷R1与人参皂苷Rg1、Rb1进行定量分析。     

底座连接口特征参数对显示器后壳注塑质量的影响分析

在Moldflow分析软件的基础上,对显示器后壳进行仿真研究,以翘曲变形量为质量指标,结合控制变量法进行单因素变动实验,保持注射工艺参数不变,研究显示器后壳底座连接口对制品翘曲变形的影响.对数据进行图表分析,结果表明显示器后壳尺寸定位68.58 cm(27英寸)时,底座连接口选用圆形,连接口位置距离底边26 mm,尺寸...     

The role of the symmetry and the flexibility of the anion on the characteristics of the nanostructures and the viscosities of ionic liquids☆

The transport properties of ionic liquids (ILs) are crucial properties in view of their applications in electrochem-ical devices. One of the most important advantages of ILs is that their chemical–physical properties and conse-quently their bulk performances can be well tuned by optimizing the chemical structures of their ions. This will require elucidating the structural features of the ions that fundamentally determine the characteristics of the nanostructures and the viscosities of ILs. Here we showed for the first time that the“rigidity”, the order, and the compactness of the three-dimensional ionic networks generated by the anions and the cation head groups determine the formation and the sizes of the nanostructures in the apolar domains of ILs. We also found that the properties of ionic networks are governed by the conformational flexibility and the symmetry of the anion and/or the cation head group. The thermal stability of the nanostructures of ILs was shown to be con-trolled by the sensitivity of the conformational equilibrium of the anion to the change of temperature. We showed that the viscosity of ILs is strongly related to the symmetry and the flexibility of the constitute ions rather than to the size of the nanostructures of ILs. Therefore, the characteristics of the nanostructures and the viscosities of ILs, especially the thermal stability of the nanostructures, can be fine-tuned by tailoring the symmetry and the conformational flexibility of the anion.     

浅谈设备的性能与操作对胎面压出质量的影响

简要介绍了胎面压出的工作原理及工艺过程，对设备的配置及操作对胎面压出质量的影响进行了分析，并对压出过程中常见的质量缺陷、造成的原因以及解决措施提出了自己的见解。     

Investigation of the effects of fatty acids on the compressive strength of the concrete and the grindability of the cement

In cement industry, a great energy consumption has been observed during grinding of clinker. To reduce this consumption, some waste products have been used as grinding aids.In this investigation, the effects of sunflower oil (SO), oleic acid (OA), stearic acid (SA), myristic acid (MA) and lauric acid (LA) on the fineness and strength of the cement have been examined. These aids were added into clinker in certain ratios based on the cement clinker weight and the grinding has been done for a definite time at the same condition.All of the fatty acids used increased the fineness as compared with the cement without the grinding additives. SO and OA decreased the strength significantly, LA decreased it to a lesser extent and SA increased it definitely according to the common cement. But MA did not alter the strength of the cement as much as SA. In addition, the covering of the balls influences the grinding of cement clinker unfavourably.     

煤粉流动性对GSP气化炉气力输送的影响研究

针对GSP气化装置煤粉气力输送在试车阶段出现的煤粉锁斗下料不畅、发料罐输送性能不佳等问题,从原料煤性质和工业装置的改进方面出发,通过煤粉的流动性参数的测量与研究,分析出现上述工程问题可能的内在原因,并提出工业装置改进方法,为保证GSP煤粉气力输送工业装置的正常运行提供解决办法。     

关于建立火炸药安全数据库的探讨

由反映火炸药及其制品的危险性能和用于对它们的生产、储运系统进行安全评价数学模型出发,探讨了如何建立一个全面、实用的火炸药安全评估数据库。通过对此数据库内容、数据表示方法、数据搜索方法以及建立步骤的讨论,明确了该数据库的使用对象和主要功能。     

The effect of the length of the oxyethylene chain on the conductivity of the polyurethaneurea electrolyte

Polyurethaneureas (PUU), which were synthesized from 4,4′-diphenylmethane diisocyanate (MDI), poly(ethylene glycol) (PEG), and 3,5-diaminobanzoic acid (DABA), were used as polyelectrolytes in this study. Differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR) were used to monitor the effect of the various kinds of PEG on the changes in morphology of PUU electrolytes corresponding to the concentration of lithium perchlorate (LiClO₄) dopants. The results of DSC and FT-IR indicate the Li⁺ ions coordinate with the soft and hard segments. Additionally, the crystallinity of the PEG soft segment and the ordered hydrogen-bonded urea carbonyl groups decreased with increasing salt concentration. Impedance spectroscopy (IS) measurements show that the PUU electrolyte with the high phase separation degree has the high ionic conductivity. The hard-segment T_g and the soft-segment T_m influence the conductivity behavior of polyelectrolytes with increasing measurement temperature.     

转子挤压组合凹模的应力分析

组合凹模的应力分析是一个非常复杂的问题，对其进行精确分析是解决模具寿命的关键。本文采用有限元分析软件Ansys对转子的组合凹模进行了分析，为转子组合凹模的优化设计提供了依据。     

Effect of the drafting force on the strength of the bottom of the spinneret

In spinning basalt fibres, the drafting force is in the same range as in spinning of glass fibres. The effect of the drafting force can not be considered in the calculation for the strength and rigidity of the bottom of the spinneret. __________ Translated from Khimicheskie Volokna, No. 5, pp. 47–50, September–October, 2007.