Water at room temperature as a solvent for the extraction of apple pomace phenolic compounds

A fractionation method was used to extract phenolic compounds from apple pomace (AP) involving a first extraction with water and subsequent extractions of the same residue with two different organic solvents. The water extracts obtained contained high amounts of phenolic compounds with high antioxidant capacity. However, the second and third extractions of the same residue still extracted considerable amounts of remaining phenolic compounds, both with significant antioxidant capacities. Liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI/MS) studies showed water to be a good solvent to extract hydroxycinnamic acids, flavonols, flavanols, dihydrochalcones and flavones present in the AP. However, water was not the ideal solvent to extract the quercetin glycosides.     

Effect of high-pressure processing on flavonoids,hydroxycinnamic acids,dihydrochalcones and antioxidant activity of apple ‘Golden Delicious’ from different geographical origin

The influence of high-pressure processing (HPP) (400, 500 and 600 MPa at 35 °C for 5 min) on different classes of phenolic compounds and antioxidant activity (AA) of ‘Golden Delicious’ apple from two different growing regions, northeastern of Spain (lowland climate) (S-apples) and north of Italy (mid-mountain climate) (I-apples) was investigated. Total hydroxycinnamic acids, total dihydrochalcones and total flavan-3-ols content were higher in S-apple (untreated and HPP-treated) than in I-apples and total flavonols content was higher in I-apples. HPP affected phenolic compounds and AA depending on the apple geographical origin. 400 MPa/35 °C/5 min increased total flavonols (30%) and maintained total phenolic compounds determined by HPLC (TP-HPLC) in S-apples. The higher increase of TP-HPLC (54%) was achieved when I-apple was treated at 600 MPa. Untreated and HPP-treated I-apples displayed higher AA than S-apples. HPP (400 and 600 MPa) increased AA in I-apple. Positive correlations were found between TP-HPLC and AA (TP-FC, DPPH^·, ABTS^·+ and FRAP) in both Italian and Spanish apples.Industrial relevanceThe apples of cultivar ‘Golden Delicious’ are one of the most consumed fruits in the UE. High-pressure processing (HPP) of these fruits acquires great importance to obtain ingredients and apple functional foods highly demanded by consumers. For this, it is necessary to know the process variables and plant material that favor greater extraction of phenolic compounds and antioxidant activity characteristics. This paper provides useful results to help fruit processor to select the appropriate HPP conditions and the geographical origin of ‘Golden Delicious’ apple to obtain apple-based products with high content on different classes of phenolic compounds with beneficial health effects.     

中早熟苹果品种的贮藏品质和酚类物质差异分析

中早熟苹果品种可以丰富水果市场,是苹果产业重要的组成部分,研究其贮藏特性及品质差异意义重大。本实验以‘鲁丽’‘鲁艳’两个苹果新品种为研究对象,以其亲本‘嘎啦’为参照,探究3种苹果的贮藏品质和酚类物质差异。结果表明：相比于‘嘎啦’苹果,‘鲁丽’苹果可溶性固形物质量分数最高,果皮a*值最大,外观品质最佳、适于鲜食,而‘鲁艳’苹果总酸度高、固酸比低,贮藏期间乙烯释放量高,不耐贮藏。与‘嘎啦’和‘鲁艳’苹果相比,‘鲁丽’苹果有着最高的总酚、总黄酮含量和1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基清除率,抗氧化能力最强。3种苹果主要的酚类物质也因品种、成熟度和果实部位存在差异,‘嘎啦’和‘鲁丽’苹果果肉均以绿原酸含量最高,而‘鲁艳’苹果表儿茶素含量最高,果皮酚类物质含量高于果肉。相关性和主成分分析表明果实硬度与a*值有高度显著负相关性（P<0.001）,可溶性固形物质量分数与总酚、总黄酮、绿原酸含量和DPPH自由基清除率存在高度显著正相关（P<0.001）,主成分1主要由总酚、总黄酮、绿原酸、表儿茶素含量等指标决定,成熟...     

INFLUENCE OF CONVENTIONAL CLARIFICATION AND ULTRAFILTRATION ON THE PHENOLIC COMPOSITION OF GOLDEN DELICIOUS APPLE JUICE

Abstract The effects of conventional clarification and ultrafiltration techniques on the phenolic composition of apple juice was studied. Since the health benefit of fruits and fruit juices have been ascribed, in part, to the phenolic antioxidants, it is very important to know what the effects of processing steps are on the level of phenolic compounds in fruit juices. The total losses of phenolic compounds were determined to be 28% and 7% during conventional clarification using gelatin and bentonite, and ultrafiltration, respectively. A total of 14 phenolic compounds were identified in apple juice prepared from Golden Delicious apples. The phenolic compounds were found to be based mainly on hydroxycinnamic acids (∼ 50% of total phenolics) and chlorogenic acid was found to be the major phenolic compound with its concentration as high as 465.45 mg 1⁻¹ in raw apple juice.     

Comparative study of primary and secondary metabolites in apricot (Prunus armeniaca L.) cultivars

BACKGROUND: Apricot is one of the most popular Prunus species grown in the temperate zone and great variation in the contents of primary and secondary metabolites has been reported in its fruit. However, little is known on the phenolic profiles and antioxidant potential of important local cultivars. The objective of the study was to identify valuable parental lines for breeding of cultivars with high health‐promoting effects. RESULTS: Primary metabolites (sugars, organic acids) and secondary metabolites (phenolics) were quantified by high‐performance liquid chromatography–mass spectrometry (HPLC/MS) in fruit of 13 apricot cultivars: total sugars ranged from 59.2 to 212.5 g kg^?1 fresh weight (FW) and total organic acids from 4.2 to 20.8 g kg^?1 FW. Four hydroxycinnamic acids and three flavonols were quantified; their content was significantly higher in skin compared to pulp. Similarly, antioxidative potential was significantly higher in skin and ranged from 125.4 to 726.5 mg ascorbic acid equivalents kg^?1 FW. A positive correlation between total phenolic content and antioxidant potential was determined. Multivariate analysis subdivided the cultivars into five major groups, mostly influenced by the content of individual and total phenolics as well as the antioxidant potential of apricot skin and pulp. CONCLUSION: In regard to high phenolic content in connection with antioxidant potential, three cultivars stand out as the richest in analyzed quality parameters: ‘Mula Sadik’, ‘Bergeron’ and ‘Chuan Zhi Hong’. They also contained high amounts of primary metabolites and should be promoted for further planting and included in apricot breeding programs. Copyright © 2010 Society of Chemical Industry     

Impact of shelf life on content of primary and secondary metabolites in apple (Malus domestica Borkh.)

In this study, we evaluated the changes in apple fruit quality during shelf life. After a month of cold storage, apples of cultivars "Jonagold" and "Golden Delicious" were exposed to ambient temperatures for 21 d, with subsequent sampling every 3 or 4 d. Fruit firmness, changes in amounts of sugars, malic acid, and phenolics were observed during shelf life. Chemical analyses were done with HPLC-PDA system. An interchange between various sugars was noticed, but in general, the sum of sugars remained at the same level. The content of malic acid remained stable or dropped, resulting in sweeter fruit. Levels of phenolics were more constant in the pulp of both cultivars analyzed, while in the peel, the changes were more pronounced. In the pulp, a peak in the content of hydroxycinnamic acids and flavanols was noticed on the 2nd or 3rd sampling and afterwards, the amounts remained constant. In the peel an initial decrease of all analyzed phenolic groups was observed in both cultivars, however it was more pronounced in "Jonagold." It can be concluded that changes in primary and secondary metabolites are not the main reason for the lower quality of fruit exposed to ambient temperatures. On the other hand, fruit firmness might be the limiting factor for shelf life duration.     

Phenolic compounds in some apple (Malus domestica Borkh) cultivars of organic and integrated production

Eleven organically grown apple cultivars and 11 apple cultivars of integrated production from Austria and Slovenia were analyzed by HPLC for the content of phenolic compounds in peel and pulp. We identified chlorogenic acid, p‐coumaric acid, procyanidin B3, protocatechuic acid, (?)‐epicatechin, phloridzin, rutin and quercetin‐3‐rhamnoside in apple peel. In apple pulp, (+)‐catechin was also identified in all the cultivars. Some other phenols (procyanidin B3, rutin and quercetin‐3‐rhamnoside) could not be identified or were not properly separated. With regard to the phenolic content in the apple peel, there were no differences between organically grown apple cultivars and apple cultivars of integrated production. Organically grown apples, however, exhibited a higher content of phenolic substances in the apple pulp compared with the apple cultivars of integrated production. This may be due either to the different genotype source or to the growing technology. Higher concentrations of phenolic compounds in organically grown cultivars could be a result of plant response to stress. The apple peel contained higher concentrations of identified phenols than the pulp. The apple peel represents up to 10% of the whole fruit; therefore the phenolsic compounds in the pulp are of greater importance to the consumer than the phenolic compounds in the peel. Copyright © 2005 Society of Chemical Industry     

Antioxidant capacity and phenolic content in blueberries as affected by genotype and growing season

Blueberry fruits from five commercial cultivars and 13 breeding selections grown at the same locations over two growing seasons were analysed for total phenolics (TPH), total anthocyanins (ACY), total hydroxycinnamic acids (HCA), total flavonols (FLA), fruit weight and oxygen radical‐absorbing capacity (ORAC). Variation in ORAC, TPH, ACY, HCA, FLA and fruit weight among genotypes was much greater than that observed between growing seasons, indicating that genetics plays a more important role than growing season in influencing ORAC and phenolic content in blueberries. Significant main effects for growing season and genotype × growing season observed for ORAC, ACY, HCA and fruit weight demonstrate that environmental growing conditions can impact levels of phenolics and ORAC in blueberries and that certain genotypes vary in their capacity to synthesise phenolics under different growing conditions. In general, genotypes with smaller berries had higher ORAC values and levels of TPH, ACY, HCA and FLA than large‐berried genotypes. Over both growing seasons, ORAC correlated highly with TPH, ACY, HCA and FLA, while fruit weight correlated inversely with all phenolics measured. Our results indicate that blueberry genotypes should be screened over multiple growing seasons in order to identify antioxidant‐ and phenolic‐rich germplasm. Copyright © 2003 Society of Chemical Industry     

Comparative polyphenolic antioxidant profile and quality of traditional apple cultivars as affected by cold storage

‘Kathista’ and ‘Lortiko’ are regarded as traditional and highly appreciated apple cultivars in Cyprus, yet their postharvest behaviour and phytochemical content are largely unknown. Such fruits were examined for their qualitative traits, phenolic composition and antioxidant capacity after harvest or short, intermediate or extended cold storage and additional ripening at room temperature (RT), using ‘Gala’ as reference cultivar. Total phenolic content was dependent on cultivar and length of cold storage, while hydroxycinnamic acids were always at higher levels compared to total flavonols for all the examined cultivars. No clear trends regarding the influence of cold storage or RT maintenance on fruit antioxidant properties were evident. Overall, quality attributes and polyphenolic content of the traditional apple cultivars were comparable with ‘Gala’ at harvest; thus, they can be considered a good source of nutraceuticals as summer apple cultivars. However, extended cold storage deteriorates fruit behaviour and antioxidant capacity, particularly in ‘Lortiko’ fruit.     

Effects of various clarification treatments on phenolic compounds and color of apple juice

The purpose of this study was to investigate the changes that occur during conventional clarification using gelatin, bentonite, silica sol, and water-soluble chitosan on the phenolic compounds, antioxidant activity, and color of apple juice. The apple material used in this study was of two varieties: Sampion and Idared. The changes in the polyphenols composition (procyanidins, hydroxycinnamic derivatives, and dihydrochalcones) were monitored through the clarification process. Sampion apple control juices contained more total polyphenols than do Idared apple juices. In Sampion variety apple juice, the dominant polyphenols are the flavan-3-ols (86% of total polyphenols), followed by hydroxycinnamic acids (9.7%), dihydrochalcones (3.0%), and flavonols (1.3%). In Idared apple juice the hydroxycinnamic acids (especially chlorogenic acid) are dominant (about 48% of total polyphenols), followed by flavan-3-ols (40%). However, the concentration of polymeric procyanidins in Sampion apple juices was 62.8 and 46.3% less when the Profloc (chitosan) and gelatin treatments were used, respectively. Aktivbentonit and Puranit (bentonite) supplementary added in juices clarification have some protective effect on polymeric procyanidins only with Profloc treatment. That kind of effect was not observed in Idared apple juices with almost eight times smaller polymeric procyanidins concentration than in Sampion apple juices. The antioxidant activity, measured by the DPPH (1,1-diphenyl-2-picrylhydrazyl radical) method, ranged from 0.20 mg TEAC/mL in Idared apple juice to 0.30 mg TEAC/mL in Sampion apple juice, measured by the ABTS method, from 0.17 to 0.48 mg TEAC/mL, respectively. Clarification of apple juices with chosen clarifying agents has statistically no significant (p>0.05) influence on antioxidant capacity. This study suggests that chitosan can be used as a conventional clarifying aid of apple juices and that treatment has no impact on their biochemical parameters.     

Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy

The fruit quality characteristics, phenolic compounds and antioxidant capacities of 24 sweet cherry (Prunus avium L.) cultivars grown on the mountainsides of the Etna volcano (Sicily, Italy) were evaluated. High-performance liquid chromatographic methods were used to identify and quantify sugars, organic acids and phenolics. A total of seven phenolic compounds were characterised as hydroxycinnamic acid derivatives (neochlorogenic acid, p-coumaroylquinic acid and chlorogenic acid) and anthocyanins (cyanidin 3-glucoside, cyanidin 3-rutinoside, pelargonidin 3-rutinoside and peonidin 3-rutinoside). The total anthocyanin content ranged from 6.21 to 94.20 mg cyanidin 3-glucoside equivalents/100 g fresh weight (FW), while the total phenol content ranged from 84.96 to 162.21 mg gallic acid equivalents/100 g FW. The oxygen radical absorbance capacity (ORAC) assay indicated that fruit of all genotypes possessed considerable antioxidant activity. The high level of phenolic compounds and antioxidant capacity of some sweet cherry fruits implied that they might be sources of bioactive compounds that are relevant to human health.     

Polyphenolic profile and antioxidant activity of five apple cultivars grown under organic and conventional agricultural practices

The polyphenols and total antioxidant activities of five apple cultivars, grown by organic and conventional agricultural methods in neighbouring farms, were determined and compared. Total polyphenols in the whole fruit and in the peel were determined by the Folin-Ciocalteu method, and the total antioxidant activity was determined by three established methods, diphenyl picrylhydrazyl (DPPH), azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and ferric reducing/antioxidant power (FRAP). Polyphenolic content for the whole fruit was in the range of 80–196 and for the peel 165–400 (mg Gallic Acid Equivalent (GAE)/100 g fresh weight) for both types of agricultural practices. Antioxidant activities of fruit extracts were also relatively similar and well correlated to their polyphenolic content. High-performance liquid chromatography (HPLC) analysis of the most important polyphenolics (chlorogenic acid, catechin, epicatechin, procyanidin B1 and B2, cyaniding 3-galactoside, phloridzin, quercetin 3-galactoside and quercetin 3-arabinoside) also showed that concentrations do not differentiate significantly between the organic and conventional apples. Statistical significance of differences in antioxidant activities among the same cultivars was relatively small (flesh + peel or peel only) for both types of apples. These results indicate that organic apples do not present higher antioxidant or nutritional value compared with conventionally grown ones, as far as polyphenolic content and total antioxidant activities are concerned.     

Polyphenol content and antioxidative activity in apple purées with rhubarb juice supplement

The purpose of the present study was to evaluate the effect of rhubarb juice addition and peeling in apple purée production and storage on its phenolic composition, antioxidant activity and colour. The apple material used in this study was of two varieties: Sampion and Idared. The apple purées prepared in three variants (control purées, peeling purées and purées with 5% of rhubarb juice) were stored for 3 and 6 months at 30 °C. Apple purées were investigated for their antioxidant activity, change of colour and contents of phenolic compounds plus the polymer procyanidins and degree of polymerization (DP). The preparation of purées from apples without peeling and the addition of rhubarb juice during purée preparation had significant influence on polyphenol content. The purée prepared from peeled apples of Idared variety had phenolic content 2.2 times lower than the control sample (non‐peeled apples). The highest level of total polyphenols was found in Idared + rhubarb sample (129.82 mg 100 g^?1 purée). The content of this compound in Idared control sample was more than 2.8 times lower than in Idared purées with rhubarb. The use of non‐peeled Idared apples and 5% of rhubarb juice in purée preparation significantly increased polymeric procyanidins from 11.68 to 75.20 mg 100 g^?1 and other phenolics from 9.23 to 54.62 mg/100 g. This effect for Sampion apples was smaller. The procyanidins during purées storage were more stable in samples with rhubarb juice addition. The purée samples with the addition of rhubarb juice had higher antioxidant activity measured using ABTS (2,2′azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonic acid)) than purée samples without the addition (two times higher for Idared variety) of rhubarb juice. After 3 and 6 months of storage at temperature 30 °C, the antioxidant capacity decreased in all purées. Rhubarb juice had only high and positive effect on L* value in the case of Idared variety. The results showed that all Sampion apple purées had higher L* values than Idared.     

Monomeric phenolics in different parts of high‐acid apple (Malus sieversii f. niedzwetzkyana (Dieck) Langenf): a promising source of antioxidants for application in nutraceuticals

The high‐acid apple (Malus sieversii f. niedzwetzkyana (Dieck) Langenf) possessing deep red peel and light red flesh is widely distributed in China. To determine if a useful apple variety is being ignored, the monomeric phenolic profiles and antioxidant activities of peel, flesh, pomace, whole fruit and juice were evaluated. The results were compared to those of ‘Fuji’ and ‘Granny Smith’ apples. The high‐acid apple possessing the highest total phenolic and anthocyanin contents among three apple varieties contained the most chlorogenic acid, phloridzin, epicatechin, ferulic acid, catechin, vanillic acid, p‐coumaric acid and caffeic acid. The extraction yields of phenolics (64%) and anthocyanins (89%) from the high‐acid apple to juice were the highest. The high‐acid apple possessing the greatest antioxidant activity had the strongest antihaemolysis activity, indicating that it could be used to produce juice and value‐added ingredient to assist in the prevention of chronic disease.     

Varietal differences among the phenolic profiles and antioxidant properties of four cultivars of spine grape (Vitis davidii Foex) in Chongyi County (China)

Spine grape (Vitis davidii Foex) is an important wild plant species in South China. To provide sufficient experimental evidence for the strong antioxidant activity of spine grapes, four cultivars from Chongyi County, China, including three red varieties (Junzi #1, Junzi #2, and Liantang) and one white variety (Baiyu) were evaluated. The Junzi #1 had the highest phenolic content (total phenolic, flavonoids, flavanols, and anthocyanins) and the strongest antioxidant capacity (DPPH radical-scavenging capacity, cupric-reducing capacity and hydroxyl radical-scavenging activity) among the four varieties. HPLC analysis of spine grapes revealed that the (+)-catechin was the most abundant phenolics and the hydroxycinnamic acids were the major phenolic acids in the four varieties. Hierarchical cluster analysis showed that the Junzi #1 belongs to the group with high phenolic content and strong antioxidant power. The results suggest the Junzi #1 has the best health promoting properties, and the higher utilization value and potential for development.     

Phenolic and antioxidant composition of by-products from the cider industry: Apple pomace

Eleven different cider apple pomaces (six single-cultivar and five from the cider-making industry) have been analysed for low molecular phenolic profiles and antioxidant capacity. The Folin index ranged between 2.3 and 15.1 g gallic acid per kg of dry matter. Major phenols were flavanols, dihydrochalcones (phloridzin and phloretin-2′-xyloglucoside), flavonols and cinnamic acids (chlorogenic and caffeic acids). The group of single-cultivar pomaces had higher contents of chlorogenic acid, (-)-epicatechin, procyanidin B2 and dihydrochalcones, whereas the industrial samples presented higher amounts of up to four unknown compounds, with absorption maxima between 256 and 284 nm. The antioxidant capacity of apple pomace, as determined by the DPPH and FRAP assays, was between 4.4 and 16.0 g ascorbic acid per kg of dry matter, thus confirming that apple pomace is a valuable source of antioxidants. PLSR analysis gave reliable mathematical models which allowed to predict the antioxidant activity of apple pomace as a function of the phenolic profile. The variables with the higher modelling power were phloridzin > procyanidin B2 > rutin + isoquercitrin > protocatechuic acid > hyperin.     

Effect of methyl jasmonate on quality and antioxidant activity of postharvest loquat fruit

BACKGOUND: Loquat fruit is rich in natural antioxidants and has shown a remarkably high antioxidant activity. To search for an effective method for maintaining or even improving antioxidant activity during postharvest storage, we investigated the effect of 10 µmol L^?1 methyl jasmonate (MeJA) treatment on levels of major individual sugars and organic acids, total phenolics, total carotenoids, total flavonoids and antioxidant activity in loquat fruit during storage at 1 °C for 35 days. RESULTS: The MeJA‐treated fruit exhibited significantly lower levels of respiration rate, ethylene production, phenylalanine ammonia‐lyase and polyphenol oxidase activities, and higher levels of sugars, organic acids, total phenolics and total flavonoids than control fruit. Meanwhile, the treatment also maintained significantly higher antioxidant activity as measured by the scavenging capacity against 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), superoxide and hydroxyl radicals, and by the reducing power test compared to the control. There was a significant positive linear relationship between total phenolic content and DPPH radical scavenging activity. CONCLUSION: MeJA treatment can improve the quality and functional properties of harvested loquat fruit by maintaining a higher level of antioxidants and enhancing antioxidant activity. Copyright © 2009 Society of Chemical Industry     

Effect of l-ascorbic acid addition on quality, polyphenolic compounds and antioxidant capacity of cloudy apple juices

Polyphenols are one of the most important dietary antioxidants, and the apples, being a major source of polyphenolic compounds. There is increasing evidence that the consumption of fruit and their preserves, which are rich sources of vitamins and phenolics, is negatively associated with lung cancer and coronary heart disease. The influence of ascorbic acid addition on the quality, polyphenolic profile and antioxidant capacity in 14 selected varieties of apple juices during the 3 years of study was examined. Evaluation of changes of ascorbic acid content allowed to demonstrate the differences in susceptibility of apples on the enzymatic oxidation. The study shows that the variations of the least susceptible to oxidation of ascorbic acid are ‘Shampion’ and ‘Topaz’, and to a lesser extent, ‘Szara Reneta’, ‘Arlet’ and ‘Jonafree’ apples. A total of 17 kinds of polyphenolic compounds including catechins, procyanidins, hydroxycinnamates, flavonols, anthocyanins and dihydrochalcones were identified in apple tissues by HPLC with DAD analysis and after phloroglucinolysis and LC–MS. During the 3 years of study, the addition of ascorbic acid positively affects on the polyphenol compounds and antioxidant capacity (DPPH, ABTS and FRAP), increasing their content in the analyzed samples by 65 and 433, 328 and 300 %, respectively. The correlation coefficient between DPPH, ABTS and FRAP antioxidant capacity and ascorbic acid was 0.71, 0.71 and 0.72, respectively.     

贮藏温度对葡萄果实采后抗氧化活性的影响及动力学分析

以"甬优1号"葡萄果实为原料,探讨贮藏温度(273.15、283.15、293.15K)对果肉和果皮总酚、花色苷和抗氧化活性的影响,并应用Gomportz函数模型,对不同温度下葡萄果实贮藏期间抗氧化活性变化的动力学模型进行研究。结果表明,葡萄果实贮藏过程中果肉和果皮中的总酚含量下降,果皮中花色苷含量也呈下降趋势。0℃贮藏可显著抑制果肉和果皮中总酚含量及果皮中花色苷含量下降,保持葡萄果实较高的DPPH自由基清除能力。在Arrhenius动力学方程基础上得出葡萄果实贮藏期间果肉和果皮总酚、花色苷和抗氧化活性变化的速率常数随着贮藏温度的提高而增大,拟合所得总酚、花色苷含量和DPPH自由基清除能力一级动力学模型回归方程的决定系数均大于0.91。由果肉和果皮总酚、花色苷和抗氧化活性预测模型所得各抗氧化指标预测值与实测值之间的平均相对误差均小于10%,表明在贮藏温度273.15～293.15K(0～20℃)范围,可预测葡萄果实采后抗氧化物质含量及抗氧化能力的变化。     

不同品种枣果实发育过程中多酚类物质、VC含量的变化及其抗氧化活性

为探索不同品种枣果发育过程中多酚类物质与抗氧化活性的动态变化规律,对灰枣、鸡心枣、梨枣、赞皇枣、磨盘枣、葫芦枣6 个枣品种的不同发育期果实中多酚类物质的含量及组成和VC含量及抗氧化活性进行分析测定。结果表明,供试各品种枣果实中总酚、总黄酮、VC含量和抗氧化活性均在采摘初期（幼果期）为最高,其后随着果实的发育呈逐渐下降趋势。其中总酚、总黄酮含量在绿熟期前下降迅速,绿熟期以后变化趋缓,果实接近成熟时则趋于平稳,全红期果实可较采摘初期降低60%以上;VC含量和抗氧化活性在白熟期前下降较快,白熟期后降幅变小。高效液相色谱（high performance liquid chromatography,HPLC）分析结果显示,儿茶素、表儿茶素、芦丁是供试各品种枣果实中最主要的酚类物质,这些酚类物质含量在枣果实发育过程中也呈下降趋势,但在不同品种间存在较大差异。供试枣果实抗氧化活性与其中的总酚、总黄酮、VC以及儿茶素、表儿茶素、芦丁等主要酚类物质的含量均存在极显著相关关系（P＜0.01）,其中尤以芦丁、表儿茶素、儿茶素与抗氧化活性的相关性较高,说明芦丁、表儿茶素、儿茶素等多酚类物质是枣果中最主要的抗氧化活性成分。磨盘枣、葫芦枣具有较高的多酚类物质含量和抗氧化活性,鸡心枣具有较高的VC含量,是功能性枣果及其加工制品生产的理想品种。