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1.
生物医用钛植入体的表面微观形貌及化学组成作为影响植入体生物相容性的重要因素,决定了植入的稳定性和使用寿命,得到广泛研究,对钛植入体的表面改性研究现状进行系统梳理变得极为重要。针对钛植入体表面微纳米复合结构的构建及添加典型生物活性离子的研究现状进行综述,以及二者的结合对促进细胞黏附、增殖、分化和促进动物体内成骨的协同效应,简述微纳米复合结构对细胞行为的内在调控机制。结果表明,钛植入体表面的微纳米复合结构及生物活性离子对细胞的行为均表现出积极作用,兼具二者的植入体能够更好地促进细胞的黏附、增殖及分化,植入动物体内后更有利于植入体与周围组织的骨性整合。最后,根据当前生物医用钛植入体表面改性研究中存在的抗菌性能较差、对细胞的影响机制不明确等问题,提出植入体在表面改性领域的研究趋势。提出了钛植入体表面改性领域微纳结构构建和生物活性离子添加的研究现状和未来的发展方向,填补了钛植入体表面改性领域目前缺少综述文章来引领的空白,可为未来钛植入体的表面改性的发展提供借鉴。 相似文献
2.
表面生物活性涂层构建是提升金属内植物骨整合能力的有效途径,本研究利用电化学沉积技术在多孔钽支架表面构建生物活性羟基磷灰石(HA)涂层。通过接触角和比表面积测试发现,HA涂层的构建显著提升了多孔钽表面亲水性,并增加了其比表面积。利用模拟体液浸泡试验评估支架生物活性,发现仅浸泡3天后,多孔钽支架表面就已被类骨磷灰石沉积所覆盖。建成骨细胞培养模型,通过激光共聚焦观察及细胞增殖测试发现,所有支架均具有良好的细胞相容性。并且,细胞共培养5天后,HA涂层化多孔钽支架表面细胞的增殖率分别是未改性材料组和空白对照组的1.1和1.4倍,呈现了更大的促细胞增殖潜力。本研究中所制备的生物活性多孔钽支架具备快速诱导类骨磷灰石沉积能力,能够促进成骨细胞在其表面的贴附和增殖,在骨修复领域具有较大的临床应用前景。 相似文献
3.
Y. Yang N. Oh Y. Liu W. Chen S. Oh M. Appleford S. Kim K. Kim S. Park J. Bumgardner W. Haggard J. Ong 《JOM Journal of the Minerals, Metals and Materials Society》2006,58(7):71-76
Osseointegrated dental implants are used to replace missing teeth. The success of implants is due to osseointegration or the
direct contact of the implant surface and bone without a fibrous connective tissue interface. This review discusses the enhancement
of osseointegration by means of anodized microporous titanium surfaces, functionally macroporous graded titanium coatings,
nanoscale titanium surfaces, and different bioactive factors. 相似文献
4.
孔隙率对多孔钛的成骨性能影响较大,高的孔隙率更有利于骨组织的长入。但随着孔隙率的升高,其力学性能必然会急剧下降。因此,如何在保证多孔钛高孔隙率的前提下提高其力学性能,成为当前势必解决的难题。本研究采用浆料发泡法,通过在钛粉中加入不同含量的氧化铈,制备出高孔隙率的多孔钛。结果表明,多孔钛孔隙呈三维网络状,孔隙率为71.6%~73.5%,孔径主要分布在100~700μm,且孔壁上分布着微米级的微孔。当氧化铈的加入量为0.2%(质量分数,下同)时,多孔钛表现出最优的生物力学相容性,其杨氏模量为2.08GPa,抗压强度为60.19MPa。 相似文献
5.
目的 提高钛种植体的生物相容性能和抗菌性能。方法 用多巴胺包覆和表面引发聚合法将季铵化聚乙烯亚胺(QPEI)接枝到酸蚀钛片表面。通过扫描电子显微镜、水接触角测试、X射线光电子能谱(XPS)、激光共聚焦、细胞实验、动物实验等方法,分别评价修饰材料的表面形貌、亲水性、结构、抗菌性、细胞毒性、生物相容性和抗感染性能。结果 聚乙烯亚胺(PEI)上的叔氨基成功转变为季铵基团,通过多巴胺包覆及表面引发聚合的方法均可以在种植体表面成功接枝QPEI,但其抗菌性能和细胞相容性存在差异,通过表面引发聚合进行表面功能化的Ti-PGED-Q组的抗菌性能显著优于通过多巴胺自聚进行功能化的Ti-PDA-Q组,其抑菌率为89.69%。表面引发聚合修饰能影响早期细胞粘附,但对细胞增殖没有显著影响,并显著抑制了带菌种植体造成的体内炎症反应,材料在体内埋植7天时,其表面抑菌率为85.4%,起到良好的抗菌作用。结论 通过原位聚合法对种植体表面进行修饰,可以提高其表面的抗菌性能,并保持良好的生物相容性。 相似文献
6.
聚醚醚酮是一种热塑性材料,凭其良好的生物相容性和X射线可透射性,被广泛用于生物医学领域。而PEEK骨植入体与人体骨骼强度存在一定差距,与人体骨组织结合能力较差和抗菌性能等不足,使其在生物领域的应用受到了限制。为了使PEEK更好地应用于人体骨植入领域,获得优异生物性能的PEEK骨植入体已成为研究的重点。概述了PEEK的加工技术、力学性能、骨整合性能和抗菌性能的研究。在此基础上,重点综述了近年来提高PEEK骨植入体力学性能与生物性能的各种改性方法的研究进展。在力学性能方面,对PEEK常用的填充材料碳纤维进行了概述,由于PEEK与碳纤维界面结合强度影响其整体力学性能,重点介绍了提高其结合强度的改性方法。在骨整合性能方面,对钛、二氧化钛和羟基磷灰石涂层材料及喷涂方法进行了概述,以及对等离子喷涂、喷砂、激光蚀刻和浓硫酸刻蚀表面处理方法的优缺点进行了分析。在抗菌性能方面,银离子释放浓度过高时会导致细胞毒性,重点阐述了如何控制银离子释放速度的研究。最后展望了PEEK骨植入体加工和改性的未来发展方向。 相似文献
7.
采用四甲基偶氮唑盐微量酶反应比色法(MTT法)对微弧氧化处理的钛基种植材料的细胞毒性进行研究,并用相差显微镜对细胞形态进行了观察与分析。本研究采用原代培养鉴定后的成骨细胞,与不同材料三维培养,进行种植体材料的细胞毒性研究。结果表明:微弧氧化处理的材料表面细胞形态良好,生长旺盛,细胞计数及MTT测定结果与其它组比较,具有显著性差异,细胞毒性级别优于0级,具有良好的细胞相容性。 相似文献
8.
目的 通过3D打印技术制得钛合金,并构建出微米级多孔粗糙表面,再通过阳极氧化表面处理技术在微米级多孔粗糙表面构建出纳米级结构.方法 首先,通过高压水处理与酸蚀处理相结合的方法,对3D打印钛合金表面进行前处理,去除不良结合的球形粉末颗粒,降低3D打印钛合金表面的粗糙度及各向异性.然后,通过阳极氧化处理,在3D打印钛合金表面制备出具有纳米级孔洞的TiO2类骨膜层.结果 3D打印钛合金表面存在较大的各向异性,导致后续的电化学过程中电场放电不均匀,形成的TiO2类骨膜层稳定性差,而阳极氧化的前处理方法可有效解决这些问题.此外,纳米多孔结构抑制了阳极氧化处理中不稳定放电现象的发生,保证了膜层表面颜色的均匀性,且使膜层与钛或钛合金结合牢固,可在3D打印钛合金表面构建纳米级膜层结构.结论 该工艺方法制备的3D打印钛合金表面膜层的成膜速率和稳定性较好,膜层呈三维网状结构,大孔内附有小孔.该结构有利于细胞在其表面更好地粘附、分化和增殖,是理想的医用植入材料. 相似文献
9.
采用电子束熔化(EBM)成形工艺,制造具有可控多孔结构的Ti6Al4V植入体,分析测试其微观孔隙结构特征、孔隙率以及力学性能。扫描电镜观测结果表明,所制备的钛合金植入体孔隙结构特征与设计结构相符合,证明EBM技术能够实现钛合金植入体孔隙结构的控制;测得多孔植入体的孔隙率为60.1%,相应的抗压强度为163 MPa,弹性模量为14 GPa,与人体骨组织弹性模量相近。利用改进的碱热处理方法进行表面改性,并浸泡在模拟体液中以诱导磷灰石的形成。体外细胞培养试验结果表明,培养7 d后成骨细胞在改性的试件表面大量粘附、生长、增殖 相似文献
10.
In order to overcome the poor osteo-inductive properties of titanium implant, some methods have been used. The efforts to improve implant biocompatibility and durability by applying a hybrid technique of composite oxidation (pre-anodic and micro-arc oxidation) and hydrothermal treatment were described. Pure titanium was used as the substrate material. An oxalic acid was used as the electrolyte for the pre-anodic oxidation. A calcium and phosphate salt solution was acted as the electrolyte of micro-arc oxidation and the common pure water was used for hydrothermal treatment. X-ray diffraction (XRD), and scanning electron microscopy (SEM) have been used to investigate the microstructure and morphology of the coatings. The results show that a compact TiO2 film can be made by pre-anodic oxidation, which is effective as chemical barriers against the in-vivo release of metal ions from the implants. A porous TiO2 coating can be produced by micro-arc oxidation on titanium plate, which is beneficial to bone tissue growth and enhancing anchorage of implant to bone. De-calcium HA can be formed on the coating using hydrothermal treatment, which is similar with the primary component of bone and has a very good osteo-inductivity.The porous gradient titania coating made by the hybrid oxidation and hydrothermal treatment should show good biocompatibility in the environment of the human body. 相似文献
11.
Shuilin Wu Xiangmei Liu K. W. K. Yeung Z. S. Xu C. Y. Chung Paul K. Chu 《Journal of Materials Engineering and Performance》2012,21(12):2622-2627
Porous NiTi shape memory alloy (SMA) scaffolds have great potential to be used as orthopedic implants because of their porous structure and superior physical properties. Its metallic nature provides it with better mechanical properties and Young??s modulus close to that of natural bones. Besides allowing tissue ingrowth and transfer of nutrients, porous SMA possesses unique pseudoelastic properties compatible to natural hard tissues like bones and tendons, thus expediting in vivo osseointegration. However, the nickel release from debris and the metal surface may cause osteocytic osteolysis at the interface between the artificial implants and bone tissues. Subsequent mobilization may finally lead to implant failure. In this study, the wear properties of porous NiTi with different porosities processed at different treatment temperatures are determined. The results of the study show that the porosity, phase transformation temperature, and annealing temperature are major factors influencing the wear characteristics of porous NiTi SMA. 相似文献
12.
Jun-Lei Li Shuai Wang Fang Cao Xiao Lin Xiao-Wei Wei Zhen-Hua Zhao Xiao-Jie Dou Wei-Ting Yu Ke Yang De-Wei Zhao 《金属学报(英文版)》2019,32(9):1075-1088
Porous titanium scaffold with suitable porous architecture exhibits enormous potentials for bone defect repairs. However,insufficient osteointegration and osteoinduction still remain to open as one of the major problems to achieve satisfactory therapeutic effect. To solve this problem, many studies have been carried out to improve the bioactivity of porous titanium scaff old by surface modifications. In this study, porous Ti6Al4V scaff olds were fabricated using additive manufacturing technique. Porous architectures were built up based on a diamond pore structure unit. Alkali–acid-heat(AH) treatment was applied to create a TiO_2 layer on the porous Ti6Al4V scaff old(AH-porous Ti6Al4V). Subsequently, a hydrothermal treatment was employed to enable the formation of HA coating with nanopillar-like morphology on the alkali–acid-heat-treated surface(HT/AH-porous Ti6Al4V). The effects of surface modifications on apatite-forming ability, protein adsorption,cell attachment, cell proliferation and osteogenic gene expression were studied using apatite-forming ability test, protein adsorption assay and in vitro cell culture assay. It was found that the HT/AH-porous Ti6Al4V exhibited the highest apatite formation ability and best affinity to fibronectin and vitronectin. In vitro studies indicated that the mesenchymal stem cells(MSCs) cultured on the HT/AH-porous Ti6Al4V presented improved adhesion and differentiation compared with the porous Ti6Al4V and AH-porous Ti6Al4V. 相似文献
13.
为提高多孔医用钛合金的生物活性,以实现快速骨整合,首先利用微弧氧化法在多孔Ti3Zr2Sn3Mo25Nb(TLM)合金表面制备出含Ca、P的次级微孔涂层,并通过水热处理在此涂层表面生成羟基磷灰石.通过XRD、SEM和EDS分析了活性次级微孔涂层的相组成、微观形貌和元素特征,通过接触角测试试验对比研究了TLM合金表面改性前后的亲水性变化,并进一步通过动物试验检测了表面活性次级微孔涂层改性后多孔TLM合金的成骨性能.结果表明,微弧氧化处理可在多孔TLM表面形成规整的含Ca、P相的次级微孔层,水热处理后次级微孔层的亲水性增强,且具有良好的成骨诱导性能. 相似文献
14.
Nicholas A. Riedel Tyler B. Cote Samuel L. Bechara Ketul C. Popat John D. Williams 《Surface & coatings technology》2012
Helium irradiation of metals has long been studied in efforts to understand the damaging aspects associated with applications in fusion reactors and tritium storage. This work examines the possibility of using low energy helium ion bombardment as a method of producing a beneficial surface texturization to promote bone growth on orthopedic implants. Using 300 eV helium ions, two unique porous titanium surfaces were created when substrates were held at temperatures of roughly 450 °C and 600 °C. The surfaces were physically characterized by scanning electron microscopy (SEM) and scanning white light interferometry. A week long hFOB 1.19 cell culture was performed using an untreated titanium control to evaluate the suitability of these surfaces for orthopedic implants. Cell health and viability were evaluated by calcein AM live cell staining, MTT assay, and SEM. The results show that helium texturizations promote cellular activity and have no detrimental effect on cell health. 相似文献
15.
Titanium-based materials have been used for dental implants due to their excellent biological compatibility, superior mechanical strength and high corrosion resistance. The osseointegration of titanium dental implants is related to their composition and surface treatment. A better anti-bacterial performance of the abutment seated in the prosthetic crown is beneficial for the osseointegration and for avoiding the infection after implantation surgery. In this study, TaN-Ag coatings with different Ag contents were deposited on a bio-grade pure Ti dental implant material. A twin-gun magnetron sputtering system was used for the deposition of TaN-Ag coatings. The Ag content in the deposited coatings was controlled by the magnetron power ratio of Ag/(Ta + Ag) targets. To verify the susceptibility of implant surface to bacterial adhesion, Staphylococcus aureus, one of the major pathogen frequently found in the implant-associated infections, was chosen for in vitro anti-bacterial analyses. In addition, the biocompatibility of human gingival fibroblast (HGF) cells on coatings was also evaluated. A composite structure of crystalline TaN and Ag nanoparticles was identified. The TaN-Ag coating with the highest Ag content of 21.4 at.% possessed the lowest bacterial retention and viability of S. aureus. From the MTT assay test, the mean optical density values for the TaN and TaN-Ag coated samples after 72 h of HGF adhesion were greater than the value obtained from the uncoated Ti. The results suggested that the TaN-Ag coatings improve antibacterial performance with compatible biological response. 相似文献
16.
金属钽具有优异的生物相容性、耐腐蚀性和骨整合能力,是具有广阔发展前景的生物医用骨科材料。通过增材制造技术(AdditiveManufacturing,AM)制备的多孔钽具有精确和个性化控制的表面成分、拓扑结构、力学性能,极大地满足了不同骨缺损临床患者的需求,适合作为承重部位的骨缺损修复材料。然而,AM多孔钽仍具有表面惰性强、骨整合能力有限、无生物活性等缺陷。分别从表面改性方法和拓扑结构优化两个角度介绍了AM多孔钽骨修复材料功能化的研究进展。概述了几种典型的表面改性方法,并分析总结了其优缺点,高成本、难加工、表面惰性是限制AM多孔钽用于表面改性的主要原因。此外,拓扑结构优化也是实现多孔钽功能化的有效途径,合适的孔隙结构不仅赋予植入体与宿主骨相匹配的力学性能,同时有利于AM多孔钽与宿主建立有效的骨整合。综述了近年来AM多孔钽拓扑结构优化的研究进展,总结了孔隙率、孔径、孔隙几何形状对多孔钽力学及生物学功能的影响。最后,对AM多孔钽骨修复材料的功能化发展及临床应用提出了展望。 相似文献
17.
通过碱液处理或碱热处理并在模拟体液(SBF)中浸泡,在多孔钛表面制备了具有梯度结构的类骨磷灰石层。结果表明,碱液处理使多孔钛表面形成了网状多孔结构,组成为钛酸钠、金红石等。热处理后磷灰石在多孔钛表面的初期沉积更均匀。涂层中钙,磷、氧以及钛元素的含量随深度而变化,形成了梯度结构。具有表面梯度磷灰石涂层的多孔钛有望用于临床矫形,以提高种植体的生物活性和骨结合强度。 相似文献
18.
M. Naveed Ahsan Christ P. PaulL.M. Kukreja Andrew J. Pinkerton 《Journal of Materials Processing Technology》2011,211(4):602-609
The use of porous surface structures is gaining popularity in biomedical implant manufacture due to its ability to promote increased osseointegration and cell proliferation. Laser direct metal deposition (LDMD) is a rapid manufacturing technique capable of producing such a structure. In this work LDMD with a diode laser in continuous mode and with a CO2 laser in pulsed modes are used to produce multi-layer porous structures. Gas-atomized Ti-6Al-4V and 316L stainless steel powders are used as the deposition material. The porous structures are compared with respect to their internal geometry, pore size, and part density using a range of techniques including micro-tomography. Results show that the two methods produce radically different internal structures, but in both cases a range of part densities can be produced by varying process parameters such as laser power and powder mass flow rate. Prudent selection of these parameters allows the interconnected pores that are considered most suitable for promoting osseointegration to be obtained. Analytical models of the processes are also developed by using Wolfram Mathematica software to solve interacting, transient heat, temperature and mass flow models. Measured and modelled results are compared and show good agreement. 相似文献
19.
Endre J. Szili Sunil Kumar Mark DeNichilo Nicolas H. Voelcker 《Surface & coatings technology》2010,205(5):1630-1635
Osseointegration is a complex process governed by the interaction of many cell types including blood cells (erythrocytes, platelets and leukocytes), phagocytic cells (macrophages) and bone cells (osteoblasts and osteoclasts) on or near the implant surface. The implant surface can be modified through a variety of methods in order to achieve control of some of these cellular interactions and consequently increase the degree of implant fixation with the surrounding bone tissue. In this investigation, titanium was coated with hydroxylated silica by plasma enhanced chemical vapour deposition (PECVD) to increase the surface hydrophilicity and generate reactive surface silanol groups. Subsequently, the silica-coated titanium surface was further modified through silanisation to generate surfaces bearing different reactive chemical functionalities consisting of aldehydes, epoxides and isocyanates, which can react with the amino groups of proteins and growth factors. 2,2,2-trifluoroethylamine (FEAM) was reacted on these surfaces to determine the coupling efficiency of the different surface chemical functionalities. The amino group of FEAM can react with an amino-reactive surface functional group to form a surface terminated with 3 fluorine atoms per FEAM molecule that can be detected by X-ray photoelectron spectroscopy. By analysing the techniques used for protein attachment with the FEAM model molecule, a successful method for isocyanate/amine coupling was found and later adapted for tethering IGF-1 molecules to the functionalised PECVD silica-coated titanium surface. Therefore, this simple method of preliminary testing protein reactivity may prove to be a cost effective strategy in the development of new biomaterial surfaces modified using protein bioconjugation methods. 相似文献
20.
加速和改善骨结合过程是口腔种植领域的研究热点,电刺激成骨是其中的研究方向之一,但外源性电刺激目前还存在很多缺陷。鉴于骨本身具有压电特性,如能在钛种植体表面制备压电材料涂层,从而产生内源性电刺激来促进成骨就具有重要研究意义。BaTiO3作为一种环境友好的无铅压电材料,其四方相具备压电效应,生物相容性良好,负荷条件下可以促进成骨。本研究首先利用阳极氧化技术在钛种植体表面制备了形貌和结构优化的TiO2纳米管涂层,进而利用水热反应将TiO2纳米管涂层转化为BaTiO3涂层,通过优化实验参数并设置极限浓度探索出具有压电效应的四方相BaTiO3涂层的合成条件,并进行了表面形貌、晶相等表征和分析,结合有限元分析模拟计算验证涂层颗粒压电势。期望为种植体表面改性提供新的思路和方法。 相似文献