Poly(l-lactic acid) (PLLA) fiber mats containing two types of crude Garcinia mangostana Linn. (GM) extract [i.e., dichloromethane extract (dGM) and acetone extract (aGM)] were successfully prepared by electrospinning process. Both the neat and the GM-loaded PLLA fibers were smooth, with the average diameters ranging between 0.77 and 1.14 μm. The release characteristics of GM from the GM-loaded PLLA fiber mats were carried out by total immersion method in acetate buffer or simulated body fluid that contained 0.5 % v/v Tween 80 and 3 % v/v methanol (hereafter, A/T/M or S/T/M medium) at either 32 or 37 °C, respectively. The maximum cumulative amounts of GM released from the GM-loaded PLLA fiber mats in the S/T/M medium were greater than those in the A/T/M medium. Moreover, the cumulative amounts of GM released from the aGM-loaded PLLA fiber mats were greater than those from the dGM-loaded PLLA fiber mats in both types of medium. The antibacterial activity of the dGM-loaded PLLA fiber mats was greatest against Staphylococcus aureus DMST 20654, while that of the aGM-loaded PLLA fiber mats was greatest against S. aureus ATCC 25923 and S. epidermidis. Lastly, only the dGM-loaded PLLA fiber mats at extraction ratio of 10 mg mL?1 were toxic to the human dermal fibroblasts. 相似文献
Ultrafine 1,6-diisocyanatohexane-extended poly(1,4-butylene succinate) (PBSu-DCH) fibers were best fabricated by electrospinning from 22% w/v PBSu-DCH solution in 90:10 v/v dichloromethane/trifluoroacetic acid under the electric field of 17 kV/20 cm. The diameters of these fibers were 172 ± 3 nm. Due to their fibrous nature, the obtained PBSu-DCH fiber mats exhibited high values of advancing/receding water contact angles (i.e., 114°/79°) and porosity (69%). Indirect cytotoxicity evaluation of the PBSu-DCH fiber mats based on the viabilities of human osteosarcoma cells (SaOS-2) and mouse fibroblasts (L929) revealed that the fibrous materials did not release any substance in the level that was harmful to the cells. The potential for use of the PBSu-DCH fiber mats as substrates for bone cell culture was further evaluated in vitro with SaOS-2 in terms of the ability to support the attachment and to promote the proliferation and the differentiation of the seeded/cultured cells. Comparative studies were made against corresponding solvent-cast PBSu-DCH films. The results indicated that the bone cells grown on the surface of the fiber mats could attach, proliferate and express alkaline phosphatase (ALP), an early osteogenic proliferation marker, better than they did on the surface of the films. The evidence obtained in this work implies the potential for use of the electrospun PBSu-DCH fiber mats as bone scaffolds. 相似文献
In the present contribution, electrospinning was used to fabricate ultrafine fiber mats from poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-2-hydroxyvalerate) (PHBV), and their 50/50 w/w blend for potential use as bone scaffolds. Cytotoxicity evaluation of these as-spun fiber mats with human osteoblasts (SaOS-2) and mouse fibroblasts (L929) indicated biocompatibility of these materials to both types of cells. The potential for use of these fiber mats as bone scaffolds was further assessed in vitro in terms of the attachment, the proliferation, and the alkaline phosphatase (ALP) activity of SaOS-2 that were seeded or cultured at different times. The cells appeared to adhere well on all types of the fibrous scaffolds after 16 h of cell seeding. During the early stage of the proliferation period (i.e., from ∼24 to 72 h in culture), the viability of the cells increased considerably and appeared to be unchanged with further increase in the time in culture. In comparison with the corresponding solution-cast film scaffolds, all of the fibrous scaffolds exhibited much better support for cell attachment and proliferation. Lastly, among the various fibrous scaffolds investigated, the electrospun fiber mat of the 50/50 w/w PHB/PHBV blend showed the highest ALP activity. These results implied a high potential for use of these electrospun fiber mats as bone scaffolds. 相似文献
The aim of this work was to prepare the scaffolds of pure poly (L-lactic acid) 3% (w/v), pure chitosan 3% (w/v), and PLLA/chitosan blend (1:5) 3% (w/v) using TIPS method and investigate their properties and application in tissue engineering. An in vitro degradation study of scaffolds showed the addition of chitosan to PLLA not only increased its degradation rate, but also slowed down its pH value reduction. Addition of chitosan to PLLA increased hydrophilicity, porosity, compressive properties, and cell viability of the scaffolds. The results indicate that among all scaffolds, the most appropriate candidate for tissue engineering is PLLA/chitosan blend. 相似文献
The designing of new biodegradable polymer composites is one of the most promising areas of modern orthopedics and regenerative surgery. At present, a number of methods have been proposed for designing and processing biodegradable polymer composites via various 3D printing technologies; however, the homogeneity of filler distribution together with mechanical properties of scaffolds made of such composites are far from those required for clinical use. In this study, the method for producing biodegradable composite material based on poly(l -lactic acid) (PLLA) solution in organic solvent and hydroxyapatite (HAp) powder was proposed. The influence of HAp weight fraction and additional annealing on PLLA matrix crystallinity was investigated. It was shown that crystallinity of PLLA decreases from 58.84 ± 1.21 to 17.33 ± 1.69 as HAp weight fraction increased from 0 to 50 wt%. However, HAp filler promoted PLLA crystallites growth according to the X-ray powder diffraction analysis. The results of nanoindentation showed Young's modulus values of the 3D-printed scaffolds with 50 wt% of HAp at the level of human femur and tibia. 相似文献
Electrospun composite membranes in multiscale structures are developed for bone tissue engineering. Aligned polycaprolactone (PCL) fibers entrapping CA‐HAp microparticles (containing CaCO3, hydroxyapatite, and casein in a hierarchical organization) are electrospun to find whether synergistic effects of fiber alignment and CA‐HAp microparticles on improving osteogenic differentiation can be obtained. CA‐HAp microparticles are in a spherical morphology of 1.42 ± 0.26 µm. Their presence increases fiber diameter and does not significantly affect fiber alignment. On all membranes, adipose derived stem cells (ADSCs) from humans spread very well. On a random group, cells distribute randomly and the presence of CA‐HAp microparticles facilitates cell proliferation, especially for the one at CA‐HAp/PCL 50 wt%; the one at CA‐HAp/PCL 20 wt% shows significantly much higher alkaline phosphatase (ALP) activity (112.0% higher) than the pure PCL membrane. On aligned samples, cells align along fibers and expression of ALP is enhanced. However, at the same composition (CA‐HAp/PCL 20 wt%), the random sample has much higher ALP activity than the aligned sample. The expressions of osteogenic marker genes are also evaluated. Combining the results and the applicability of membranes together, the random membrane at CA‐HAp/PCL 20 wt% is the best candidate for bone tissue engineering. 相似文献
AbstractThe effects of poly(L,L-lactide) (PLLA) scaffold with axial and isotropic structure were investigated on functional activity of rabbit bone mesenchymal stem cells (BMSCs). PLLA scaffolds were processed by freeze-dry technique at different temperatures of the scaffold frost – ?196?°C, ?25?°C and 0?°C. Scaffolds with different pore sizes were obtained by adding 5 or 10% of water phase. Scaffolds were modified by collagen type I solution. The pore sizes of polymer scaffolds were ranging from 5 to 150?µm. More protein secretion was observed in the surface-modified scaffolds than in the unmodified after 2 weeks of cultivation in vitro. 相似文献
In an effort to enhance the properties of polylactide (PLA), we have developed melt-spinning techniques to produce both PLA/nanocellulose composite fibres, and a method akin to layered filament winding followed by compression moulding to produce self-reinforced PLA/nanocellulose composites. Poly(L-lactide) (PLLA) fibres were filled with 2 wt.% neat and modified bacterial cellulose (BC) in an effort to improve the tensile properties over neat PLA fibres. BC increased the viscosity of the polymer melt and reduced the draw-ratio of the fibres, resulting in increased fibre diameters. Nonetheless, strain induced chain orientation due to melt spinning led to PLLA fibres with enhanced tensile modulus (6 GPa) and strength (127 MPa), over monolithic PLLA, previously measured at 1.3 GPa and 61 MPa, respectively. The presence of BC also enhanced the nucleation and growth of crystals in PLA. We further produced PLA fibres with 7 wt.% cellulose nanocrystals (CNCs), which is higher than the percolation threshold (equivalent to 6 vol.%). These fibres were spun in multiple, alternating controlled layers onto spools, and subsequently compression moulded to produce unidirectional self-reinforced PLA composites consisting of 60 vol.% PLLA fibres reinforced with 7 wt.% CNC in a matrix of amorphous PDLLA, which itself contained 7 wt.% of CNC. We observed improvements in viscoelastic properties of up to 175% in terms of storage moduli in bending. Furthermore, strains to failure for PLLA fibre reinforced PDLLA were recorded at 17%. 相似文献
In an attempt to enhance the biocompatibility and mechanical strength of fibrous polymeric scaffold systems, nanocrystalline hydroxyapatite (HAp) particles were incorporated into the electrospun poly(L ‐lactide) (PLLA) fibers and then mechanically interlocked using a vapor‐phase solvent adsorption method. The solvent‐assisted compression molding substantially increased the tensile strength (from 4.61 to 12.63 MPa) and mechanical modulus (from 50.6 to 627.7 MPa) of the fibrous scaffold, which maintained the interstitial space between the fibers to allow the facile transport of nutrients and waste during cell growth and polymer biodegradation. Macrometer‐sized pores (ca. 100–400 µm) were introduced into the scaffolds in a controlled fashion using the salt leaching/gas forming technique to give desired space for a facile cell implantation and growth. Overall, the developed methodology allows the polymer‐based scaffold systems to be tailored for various applications in light of surface characteristics, mechanical strength, and pore size of engineered scaffolds.
Novel inks were formulated by dissolving polycaprolactone (PCL), a hydrophobic polymer, in organic solvent systems; polyethylene oxide (PEO) was incorporated to extend the range of hydrophilicity of the system. Hydroxyapatite (HAp) with a weight ratio of 55–85% was added to the polymer-based solution to mimic the material composition of natural bone tissue. The direct ink writing (DIW) technique was applied to extrude the formulated inks to fabricate the predesigned tissue scaffold structures; the influence of HAp concentration was investigated. The results indicate that in comparison to other inks containing HAp (55%, 75%, and 85%w/w), the ink containing 65% w/w HAp had faster ink recovery behavior; the fabricated scaffold had a rougher surface as well as better mechanical properties and wettability. It is noted that the 65% w/w HAp concentration is similar to the inorganic composition of natural bone tissue. The elastic modulus values of PCL/PEO/HAp scaffolds were in the range of 4–12 MPa; the values were dependent on the HAp concentration. Furthermore, vancomycin as a model drug was successfully encapsulated in the PCL/PEO/HAp composite scaffold for drug release applications. This paper presents novel drug-loaded PCL/PEO/HAp inks for 3D scaffold fabrication using the DIW printing technique for potential bone scaffold applications. 相似文献