共查询到18条相似文献,搜索用时 62 毫秒
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研究了鲅鱼酶解产物水溶性、持油持水能力、粘度等特性及抗氧化活性等功能性质。研究结果表明,鲅鱼蛋白在被木瓜蛋白酶酶解后(水解度25%),酶解液在p H311条件下具有很好的水溶性,水解物分子质量多集中在1350u以下,其TCA-NSI在46.0%76.8%之间,但亲油能力和水合能力均有所下降,由于较高的水解度和木瓜蛋白酶的酶切特性的影响,鲅鱼蛋白水解液的起泡性和起泡稳定性均不理想,在蛋白质浓度1080mg/m L范围内,鲅鱼蛋白水解液20℃运动粘度为1.141.82mm2/s,具有较好的流动性。鲅鱼蛋白水解产物具有较好的还原力、自由基清除能力和抗脂质过氧化能力,抗氧化活性随着浓度的增加而升高,鲅鱼蛋白水解液的抗氧化能力和其浓度间呈现剂量效应关系。本文对鲅鱼蛋白水解产物的功能性质方面的研究可为其在食品加工中的应用提供技术支持。 相似文献
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王敏李聪舒羽李皓瑜陈邦申烨华 《中国油脂》2020,45(5):67-71
研究了油用牡丹籽粕蛋白(PoSMP)及其碱性蛋白酶酶解产物(AHs)的相对分子质量分布、溶解度、变性温度及温度和pH对其抗氧化性的影响。结果表明:PoSMP的亚基主要分布在62、45、34kDa和23 k Da处,且45、23 k Da条带对碱性蛋白酶具有良好的耐受性; pH为4. 5和3. 7时,PoSMP和AHs的溶解度最低,分别为(0. 92±0. 25)%、(8. 90±0. 32)%,在中性条件下均表现出良好的溶解性;两者的变性温度分别为67. 82℃和84. 70℃。此外,PoSMP在高温和p H为3. 0~9. 0时表现出良好的抗氧化性,AHs在高温和强碱性pH 11. 0条件下也具有优异的抗氧化性。试验证明了PoSMP和AHs具有较好的溶解性及抗氧化稳定性,有望作为添加剂或功能成分应用于食品及保健品领域。 相似文献
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探究乳清蛋白在碱性蛋白酶限制性水解下功能性质变化。以乳清蛋白的溶解性,乳化性、乳化稳定性,起泡性、起泡稳定性为考察指标,确定乳清蛋白的等电点及分析不同水解度下乳清蛋白功能性质在p H调控下的变化。结果表明:乳清蛋白的等电点为4.8。乳清蛋白进行限制性酶解后功能性质有了很大提高,其中溶解性在DH14、p H10下达到最大值,较原蛋白提高了14.55%;起泡性在DH14、p H4下达到最大值,较原蛋白提高了107.5%;起泡稳定性在DH4、p H4下达到最大值,比原蛋白提高了8.66%;乳化性在DH14、p H12下达到最大值,比原蛋白提高了56.1%;乳化稳定性DH4、p H12下达到最大值,比原蛋白提高了50.42%。 相似文献
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采用不同的蛋白酶(木瓜蛋白酶、胰蛋白酶、风味蛋白酶、精制中性蛋白酶和碱性蛋白酶)水解大米蛋白,以制备抗氧化活性肽。结果发现,精制中性蛋白酶水解大米蛋白得到的产物对DPPH自由基的清除能力最强,而且水解度其清除能力影响显著。同时测定了酶解产物的还原能力、金属离子螯合能力以及在Fe2+引发的亚油酸过氧化物体系中的抗氧化活性,结果表明,此酶解产物表现出一定的还原能力,能够抑制由Fe2+引发的亚油酸过氧化物体系中的抗氧化活性,具有一定的金属离子螯合能力。结果表明,这种肽具有一定的抗氧化活性,具有良好的功能特性。 相似文献
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鸡蛋清蛋白水解物的物化及功能性质的研究 总被引:2,自引:2,他引:2
蛋清蛋白质溶解性欠佳且容易起泡,使其应用受限。本文利用Alcalase酶解鸡蛋蛋清蛋白制取水解度为5~15%的酶解物并对酶解物的理化性质和功能性质进行了表征。测定性质包括乳化性、起泡性及稳定性以及溶解性、表面疏水性。所有酶解产物具有较低的表面疏水性,水解大大提高了溶解度,当水解度15%时最大值为89%,但乳化性有所降低,起泡性及稳定性也大约下降了40%。 相似文献
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《食品与发酵工业》2014,(10):151-155
为了深入了解酶解时间和蛋白酶种类对黄鳝肉酶解产物抗氧化活性和功能特性的影响,采用木瓜蛋白酶、碱性蛋白酶和胰蛋白酶及其双酶组合在各自最适条件下对黄鳝肉进行酶解,制备不同水解度的酶解产物,并对其抗氧化活性和功能特性进行了研究。结果表明:随着酶解时间的增加,黄鳝肉酶解产物的抗氧化活性逐渐增强,当酶解时间为3 h时,酶解产物具有较强的清除DPPH和ABTS自由基的能力,最大清除率可达62.18%和81.76%,但酶解时间进一步延长,酶解产物的抗氧化能力则不断下降。在相同酶解时间下,双酶酶解产物比单酶酶解产物具有较强的清除自由基能力。黄鳝肉双酶酶解产物在溶解性和热稳定性方面优于单酶酶解产物,而在乳化性方面则相对较差,并且酶解时间对黄鳝肉酶解产物的功能性影响较大。 相似文献
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《Food chemistry》1999,65(2):189-194
Two types of soy protein hydrolysates were produced by hydrolysis of isolated soy protein (ISP) with a combination of Alcalase and Flavourzyme in a continuous membrane system with 3000 and 30,000 daltons molecular-weight-cut-off (MWCO) membranes, respectively. Both hydrolysates were completely soluble over a pH range of 2–9. Their water-binding capacity increased 1.8–3.4 times at a water activity of 0.6–0.95 as compared to intact ISP. The antioxidant activity of ISP was remarkably enhanced by enzyme hydrolysis. The hydrolysate from the 30,000 daltons MWCO membrane had a much higher antioxidant activity than that from the 3000 daltons MWCO membrane. The results suggested that both selected enzymes and MWCO membrane governed the functional properties of protein hydrolysates. Long-term operation study showed that the membrane reactor system could maintain a steady production of ISP hydrolysate over 16 h. 相似文献
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采用麦芽粉分别与风味蛋白酶、中性蛋白酶和木瓜蛋白酶协同酶解豌豆蛋白,得到3种酶解物(MFH、MNH和MPH),研究酶解物的功能特性及抗氧化活性。结果表明:与豌豆蛋白相比,酶解物的溶解性、持油性和体外消化性较高;酶解后豌豆蛋白的抗氧化活性显著提高(P<0.05),MFH、MNH和MPH的DPPH·清除能力IC50值分别为5.64、7.22、8.95 mg/mL(以蛋白质质量浓度计),·OH清除能力IC50值分别为1.45、2.19、2.04 mg/mL(以蛋白质质量浓度计);与仅用蛋白酶酶解得到的酶解物相比,MNH的苦味减轻。 相似文献
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以玉米蛋白为原料,主要研究了通过复合风味蛋白酶水解制备的玉米蛋白水解物的功能特性。结果表明:玉米蛋白经复合风味蛋白酶水解后水解度为11.43%,体外实验该水解度下的玉米蛋白水解物结合胆汁酸的能力较强,相对分子质量分布在10 000~180 Da之间,水解物中有高含量的疏水性氨基酸残留。玉米蛋白水解物持水性为2.95 mL/g,持油性为2.13 mL/g,乳化性为49.96 mL/g,起泡性为126%。玉米蛋白水解物经Sephadex G-15、RP-HPLC分离纯化,对含量最高的组分F2,用MALDI-TOF/TOF MS/MS方法进行鉴定,其相对分子质量为244.2 Da,氨基酸组成为脯氨酸-谷氨酰胺。 相似文献
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台鲅鱼头酶解液制备香精 总被引:1,自引:0,他引:1
台鲅鱼头为原料,通过实验优化酶解条件得到的蛋白酶解液,再与Maillard反应制备香精,并且通过正交设计实验得到Maillard反应的的最佳条件为:葡萄糖量6%,时间为60min,温度115℃,pH为6.5。感官评定结果表明制备的香精色泽清润,同时带有一定的焦香气,无酶解液的鱼腥味。 相似文献
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Lanfang Wu Aimin Jiang Yongshuai Jing Yuguang Zheng Yuping Yan 《International Journal of Food Properties》2017,20(5):997-1006
Protein extracted from Douchi was hydrolyzed by alcalase based on a single-factor experiment and response surface methodology was employed to optimize hydrolysis conditions. The optimum conditions were determined to be: 63℃ extraction temperature, 1.4% quantities of enzyme and substrate, and 1.7 h extraction time. The optimal protein hydrolysate was subsequently subjected to ultrafiltration, and the fraction of molecular weight 10–50 kDa was identified as the most potent contributor to antioxidant activity. Amino acid analysis indicated that acidic amino acids were the most abundant in all fractions of the protein hydrolysate. Infrared spectroscopy showed there were no significant differences in secondary structure between fractions. 相似文献
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The objective of the study was to analyze the functional and nutritional properties of enzymatically hydrolyzed whey protein concentrate (WPC) and to formulate a beverage mix. WPC hydrolysates were produced using fungal protease and papain, at time intervals of 20, 40 and 60 min and were analyzed for proximate composition and functional properties. A beverage was formulated with hydrolyzed WPC, skim milk powder, cocoa, liquid glucose, sugar and vegetable fat and analyzed for physicochemical properties, sensory attributes and keeping quality. Results revealed that the protein content of WPC was 75.6% and decreased slightly on enzyme treatment (69.6%). The water absorption capacity of WPC was 10 ml/100 g and increased in enzyme treated samples from 16 to 34 ml/100 g with increase in the time of hydrolysis. Emulsion capacity (45 ml of oil/g of control WPC) showed a decreasing trend with increasing time of hydrolysis. Enzyme treatment slightly increased the foam capacity in three samples but lowered foam stability in all. The gel filtration pattern of enzyme treated samples showed an increase in low molecular weight fractions. The amino acid profile showed higher content of methionine in samples treated with enzymes, compared to the control. The in vitro protein digestibility of untreated WPC was 25% and increased in all treated samples to varying degrees (69–70%). Formulated beverage had 52% protein, 10% fat and 6.6% ash. There were no significant differences in the sensory attributes of formulated and commercial beverage. The formulated beverage could be stored well in a PET container for 30 days. 相似文献