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从大庆油田龙虎泡区块采油地层水中分离得到一株性能很好的耐盐耐温的兼性烃降解菌XDF-4,经形态观察、生理生化实验和16S rDNA基因序列分析,初步鉴定为地芽孢杆菌Geobacillus sp..该菌在45~75℃、pH 6.5~9.0、盐的质量分数0~10%下生长良好,其最适生长温度为65℃,最适盐的质量分数为 3.0%.研究发现,该菌株能以原油为唯一碳源生长并合成生物表面活性剂, 发酵7 d, 其发酵液表面张力从68.59 mN·m-1降到29.58 mN·m-1.薄层色谱和显色反应表明,XDF-4产出的表面活性剂主要包含:糖类50.26%(质量)、脂类28.47%(质量)、蛋白质15.35%(质量);其临界胶束浓度为22 mg·L-1.GC气相色谱和族组分柱色谱分析表明,烃降解菌Geobacillus sp.XDF-4作用后,原油轻质组分含量明显增加,重质组分含量降低.物理模拟实验表明,该菌可在一次水驱基础上进一步提高采收率5.69%,可有效应用于高温高盐油藏微生物驱现场实验. 相似文献
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《山东化工》2021,50(4)
研究通过逐渐提高培养液的盐浓度从含石油烃的钻井泥浆中驯化获得四株耐盐的石油烃降解菌,筛选出1株对原油降解效率高的优势菌株SW-1。经16S r RNA基因序列分析确定其系统发育地位,采用单因素实验研究环境因素对该菌原油降解率的影响,研究其对典型石油烃类物质的降解能力及降解特性。结果表明:石油烃降解菌耐受的盐度为9%;盐度为0时,菌株SW-1的原油降解率为51.49%; 16S r RNA基因序列比对结果显示,该菌株与Bacillus licheniformis MGB70112. 1核苷酸序列相似性为100%; p H值为9,温度为30℃降解效果最佳;在9%盐浓度,最佳条件下培养7 d,SW-1菌株对原油的降解率为33. 10%,对菲的降解率为46. 53%; GC-MS分析结果表明,菌株SW-1可以降解链长为C19~C28的烷烃,C19~C28烷烃的平均降解率达到18. 48%。 相似文献
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《化工进展》2017,(3)
煤化工废水是一种含盐量较高的难降解工业废水,为了提高其生化处理的效果,从某煤化工企业生产污水中分离得到一株耐盐菌CCZU-R6,经形态观察和16S rDNA序列分析,鉴定该菌株属于芽孢杆菌属(Bacillus sp.)。采用单因素实验方法考察了pH、温度、接种量、废水盐度等工艺条件对降解模拟含盐废水的影响,确定了菌株CCZU-R6处理模拟含盐废水的最适条件为pH 7.0、温度35℃,接种量500mg/L,能耐受的最高盐度约为11%。底物谱分析显示菌株CCZU-R6对苯酚、苯甲醇、甲醇等物质具有显著的降解效果。在最适降解条件下,菌株CCZU-R6处理实际煤化工含盐废水24h,COD去除率达到93%。表明该菌株对含盐煤化工废水有较强的降解能力,具有工业应用前景。 相似文献
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从青海油田附近被石油污染的土壤中分离得到一株可利用原油为唯一碳源的菌株,将其命名为X4菌株。经16SrDNA分析鉴定,该菌株与中度嗜盐菌Salinicola zeshunii strain N4T(GenBank序列号为EU056581)同源性高达99%。X4菌株的最适温度为30℃,最适盐度为8%,最适pH为6.5,最佳碳源为甘油,最佳氮源为氯化铵。该菌可产生生物乳化剂,具有较强的细胞疏水性,对正辛烷、十六烷、二甲苯等典型烃类物质具有良好的乳化能力,细胞CSH值达到60%以上。在含5%盐度的无机盐培养基中,以3g/L的柴油为唯一碳源,采用GC-MS定量分析X4菌株的烃降解特征,结果表明菌株X4培养5天后柴油的总降解率达56%,菌株X4优先降解中长链烃类;C7~C13烃类的平均降解率为64.1%,C14~C20烃类的平均降解率为52.3%,C21~C31烃类的平均降解率约26.8%。离子型表面活性剂TTAB和SDS对X4菌株生长具有较强的毒性:在浓度达到100mg/L和400mg/L时能完全抑制菌体生长;在40mg/L的浓度下,使得菌株对柴油的降解率降低到20%。而X4菌株对非离子型表活剂——吐温80和生物表面活性剂——鼠李糖脂的耐受浓度均可达400mg/L。鼠李糖脂是嗜盐菌X4菌株的合适复配表活剂。 相似文献
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一株石油烃降解菌的固定化及其降解特性研究 总被引:2,自引:0,他引:2
《化学与生物工程》2015,(11)
从石油污染盐渍土壤中筛选出一株对液蜡乳化效果明显、对原油降解率达56.8%的菌株,命名为BZ-L。经生理生化和16SrRNA序列分析,初步鉴定该菌株属于沙雷氏菌属。以海藻酸钠和活性炭为包埋剂,对该菌株进行固定化研究。结果表明,当活性炭含量为0.8%时,固定化微球的破损率最低、渗透性最好;在接种量为35.0g·L-1、NaCl浓度为6.0%时,固定化微球对原油的降解率可达61.7%;菌株BZ-L的固定化微球对原油的降解率明显高于游离菌,且比游离菌的耐盐性能更强,可用于石油污染盐渍土壤的生物修复。 相似文献
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本文利用实验室自筛的苯胺降解菌种CLX-6作为出发菌,分别研究对菌种降解苯胺起显著作用的环境因素(盐度、苯胺耐受浓度、温度、氧气等),最终优化的数据如下:菌种耐受盐度含量4%,苯胺耐受浓度为1000mg/L,最适溶氧为摇床转速50r/min,最适温度为30℃,在此优化条件下苯胺降解率86.2%。 相似文献
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以2-丙烯酰胺基-2-甲基丙磺酸(AMPS)、丙烯酸(AA)、二烯丙基二甲基氯化铵(DMDAAC)为单体,改性蒙脱土为活性填料合成了一种有机-无机复合型大温差缓凝剂(PMC-180)。利用FTIR、XRD和TG对产物进行了结构表征,并对其应用性能进行了评价。结果表明:PMC-180具有插层剥离复合结构,初始热解温度为267.27℃。含PMC-180的水泥浆在90~180℃耐温缓凝性能和抗盐性能良好,初始稠度低于30 Bc,稠化时间可调,曲线平稳无鼓包等异常现象;含PMC-180的24 h水泥石低温强度高于14 MPa,满足长封固段固井要求。PMC-180的缓凝机理及大温差适应机理为:PMC-180通过吸附屏蔽及络合抑制作用延缓Ca(OH)_2晶核、晶体生长;PMC-180片层间距随温变化及阴阳离子竞争吸附减缓低温过渡吸附,使PMC-180具有良好的大温差适应性。 相似文献
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《现代化工》2016,(7)
针对常规调驱剂在裂缝性高温、高盐油藏调驱效果差的难题,以丙烯酰胺、衣康酸、二甲基二丙烯酰胺基磺酸钠为聚合单体,以过硫酸铵和亚硫酸氢钠为氧化还原引发剂,N,N-亚甲基双丙烯酰胺为化学交联剂,锂皂石为物理交联剂,合成了一种耐温、耐盐预交联凝胶颗粒KY-2,并对其微观形貌进行了表征,分析了化学交联剂质量分数对预交联凝胶颗粒吸水性能的影响,评价了其耐温耐盐性能、抗剪切性能和调驱性能。结果表明:颗粒的吸水速率随着交联剂质量分数的增加而降低,该颗粒具有很好的耐温耐盐性和抗剪切性能,在渗透率级差为4的条件下,水驱后提高采收率幅度达15.5%。预交联凝胶颗粒KY-2对裂缝性高温高矿化度非均质油藏具有较强的适应性。 相似文献
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Based on the optimization of culture conditions for producing 2-keto-D-gluconic acid(2-KDG)by Serratia sp.BK-98 in a Erlenmeyer flask, the factors of dissolved oxygen(DO)and pH affecting 2-KDG batch fermentation in 100 L fermenter were further optimized to be 30% and 6.0 respectively.Under the DO-stat and pH-stat batch culture conditions, 2-KDG production reached 211.2 g·L-1.The kinetics of DO-stat and pH-stat batch fermentation were also investigated and the models for biomass, substrate consumption and product were established respectively based on the Logistic equation, Leudeking-Piret equation and Modified Leudeking-Piret equation.Curve fittings for the above models by using experimental data were performed by the non-linear least squares method with the software Origin 8.0.With the evaluated model parameters, the calculated values of the models and experimental data were in good agreement and the models could provide guidance for 2-KDG fermentation production. 相似文献
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Fangfang Pan Wen-lou Wang Hongju Li Xiaodong Xin Qinqin Chang Wensheng Yan Dongming Chen 《Electrochimica acta》2011,56(20):253
Core–shell LiFePO4/C nanocomposite has been prepared by a sol–gel method. The mean size of the spherical core LiFePO4 is about 30 nm, and thickness of carbon shell is about 3 nm. The bonding character on the interface of core–shell was revealed by soft X-ray absorption spectroscopy (XAS). The as-prepared sample was characterized by X-ray diffraction (XRD), Raman spectrum, Transmission electron microscope (TEM) and High-resolution transmission electron microscopy (HRTEM). Charge-discharge tests show the core–shell LiFePO4/C demonstrates high rate capability (106 mAh g−1 at 20 C) and good cycling performance (negligible capacity loss after 250 cycles). 相似文献
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Redox catalytic process involved in the paired electrosynthesis of l-cysteine and l-cysteic acid from l-cystine is investigated by cyclic voltammetric technique and also confirmed by preparative electrolysis. The cyclic voltammetric behaviour shows that in the catholyte, in situ deposited tin (Sn) surface acts as a redox catalyst for the electro-reduction of l-cystine to l-cysteine whereas in the anolyte, the electro-generated bromine acts as a homogeneous redox mediator to enhance the electro-oxidation of l-cystine. l-Cysteine hydrochloride monohydrate (l-cysteine) and l-cysteic acid are prepared from l-cystine by preparative electrolysis with high purity and high yield using graphite cathode and DSA anode. At optimum concentration of l-cystine with 1:1 concentration ratio (catholyte:anolyte), the material yield obtained for l-cysteine is above 80% and that for l-cysteic acid is close to 60% in the paired electrosynthesis process in the batch operation. Scope for further experiments in conversion efficiency is also discussed. 相似文献
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Marwa Khmaissa Bilel Hadrich Mohamed Chamkha Adel Sayari Ahmed Fendri 《Journal of surfactants and detergents》2022,25(3):361-376
The aim of this work was to optimize the production of a new lipase by a halotolerant bacterial strain Halomonas sp. C2SS100, by means of the response-surface methodology (RSM). The process parameters having the most significant effect on lipase production were identified using the Plackett–Burman screening design-of-experiments. Then, Box–Behnken design was applied to optimize lipase activity and the quadratic regression model of the lipase production was built. Indeed, the lipase yield was increased, and the value obtained experimentally (39 ± 2 U/ml) was very close to the rate predicted by the model (40.3 U/ml). Likewise, optimization of parameters by RSM resulted in 2.78-fold increase in lipase activity. These findings provide the first report on lipase production and optimization by a halotolerant bacterial strain belonging to Halomonas genus. Afterward, the biochemical properties of the produced lipase were studied for apply in oil stains removal. The crude lipase showed a maximum activity at 60°C and at pH ranging from 7 to 10. It displayed an important stability at high temperature, pH, and NaCl. Interestingly, this bacterial lipase exhibited a prominent stability toward some commercial solid and liquid detergents after 30 min of incubation at 50°C. The capability of the crude lipase to eliminate stain was ascertained on polycotton fabric pieces stained with lubricating oil. Whether with the addition of hot water alone or of a commercially available detergent, lipase is able to considerably boost the elimination of oil stains. The actual findings highlight the capacity of Halomonas sp. lipase for energy-efficient biocatalytic application. 相似文献
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Masahiro Tajima Miki Niwa Yasushi Fujii Yutaka Koinuma Reiji Aizawa Satoshi Kushiyama Satoru Kobayashi Koichi Mizuno Hideo Ohuˆchi 《Applied catalysis. B, Environmental》1997,12(4):1418
The decomposition of chlorofluorocarbons (CFCs) in the presence of water was examined over a variety of solid acid catalysts. The TiO2ZrO2 catalyst was found to have the highest activity and longest life among the catalysts examined. The activity of the TiO2ZrO2 catalysts depended upon the content of TiO2. At the contents of TiO2 from 58 to 90 mole%, the TiO2ZrO2 catalysts exhibited high activity, and these catalysts were proven to contain TiZrO4 crystal. From the study of the XRD peak intensity of the TiZrO4 crystal, it was highest on the TZ-58 which contained 58 mole% of TiO2, and decreased with increasing the content of TiO2. Furthermore, the conversion of CFC113 measured at 673 K was highest at TZ-58, and decreased gradually with increasing TiO2 content. Therefore, the TiZrO4 crystal influences the activity of decomposition of CFC113. However, the TiO2ZrO2 catalyst was gradually deactivated during the reaction due to the elimination of titanium atoms. A good relationship was found between the activity on TiO2ZrO2 catalyst and bond energy of CCl in the compounds of chlorofluorocarbons and hydrochlorocarbons, suggesting that the rate controlling step was the cleavage of CCl bond. 相似文献
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The solubility of D-p-hydroxyphenylglycine p-toluenesulfonate and L-p-hydroxyphenylglycine in different solvents was measured using a laser monitoring observation technique. The solubility data
were accurately correlated by the modified Apelblat equation, the calculated results of which were proved to show fine representation
of experimental data. 相似文献