重组酶聚合酶扩增技术检测转基因水稻中的Cry1Ab/c基因

重组酶聚合酶扩增技术(RPA)是利用重组酶和单链结合蛋白在常温下协同实现引物与模板的特异结合,以代替传统PCR热循环中的变性和复性过程的新型恒温体外核酸扩增技术。本研究基于RPA技术建立了转基因水稻Cry1Ab/c基因的检测方法,可在37℃恒温条件下快速检测到转基因水稻中的Cry1Ab/c基因,具有较好的特异性,其绝对及相对检测灵敏度分别达到100个拷贝和0.1%(质量分数),适用于基层实验室及现场快速检测转Cry1Ab/c基因水稻及其制品。     

Comparative analysis of genetically modified brown rice with conventional rice varieties for the safety assessment

Compositional analysis of genetically modified (GM) rice (OsCK1), non‐GM comparator and reference rice as commercial rice was conducted for the safety assessment. The tolerance intervals (TI) of commercial rice were also set to illustrate compositional variability. GM rice, non‐GM rice and reference rice were compared using principal component analysis (PCA) and partial least squares‐discriminant analysis (PLS‐DA) to assess the impact of genetic modification vs. environmental influence on the rice component. Seventeen components in forty‐six analysed components were significantly different between GM rice and non‐GM rice. But, the mean ranges of the seventeen components were all within those of the reference TIs. These results demonstrate that the nutrition components of GM rice (OsCK1) fell within the range of natural variability and were biologically equivalent to non‐GM rice. In addition, multivariate analysis by PCA and PLS‐DA revealed that environmental factors such as growing locations were important due to the variability of the rice metabolite profiles. These comparisons lead to the conclusion that disease‐resistant transgenic rice (OsCK1) is compositionally equivalent to and as safe and nutritious as conventional rice varieties grown commercially.     

Quantification of mCry1Ac1 and PAT proteins during growth of genetically modified rice developed in Korea

The genetically modified (GM) rice, which expresses the insecticidal toxin modified Cry1Ac1 (mCry1Ac1) and the selectable marker phosphinothricin acetyltransferase (PAT), was developed by the Rural Development Administration in Korea. In this study, we used enzyme-linked immunosorbent assay to analyze mCry1Ac1 and PAT protein levels in GM rice. The levels of expression of both transgenes in ‘Agb0101’ (Oryza sativa L. cv. Nakdongbyeo) changed significantly over time, with the plant part, and growth stage. In leaves, mCry1Ac1 expression was the highest at the tillering stage (831 μg/gdw and 301 μg/gfw) and lowest in brown rice seeds (0.25 μg/gdw and 0.19 μg/gfw). Expression of PAT was also highest in leaves at the booting stage (97.49 μg/ gdw and 32.17 μg/gfw) and lowest in polished rice (1.02 μg/gdw and 0.78 μg/gfw). Unlike PAT protein, mCry1Ac1 was not detected in polished rice seeds. As expected, expression of mCry1Ac1 and PAT proteins was not detectable in control rice plants. In general, mCry1Ac1 and PAT levels declined steadily over the course of the plant life cycle.     

转基因食品安全性评价和安全保障

本文在简述转基因食品安全性及其研究现状的基础上 ,提出的若干对应策略 ,其中重点介绍转基因食品安全性评估的基本原则———实质等同性 ,和管理措施和检测技术的应用前景     

转基因大米及其安全性评价研究进展

近年来,转基因稻米的迅速发展引起了广泛关注,尤其是与食品密切相关的营养与安全问题。分析转基因大米的基因来源及对大米品质的影响,总结转基因大米可能存在的危害及其安全性评价的方法,可使人们科学的对待转基因大米,并为研究与开发新型转基因大米提供参考。     

转基因大米育种及其食用安全性研究进展

伴随着环境的恶化及资源的短缺,世界范围内正面临着一场粮食危机,转基因育种技术也因此得以迅速发展与应用。主要介绍了转基因大米的育种技术,同时对转基因大米的食用安全性进行了论述。     

转基因食品检测技术及其安全性评价

当前转基因食品的安全性问题已经越来越受到人们的广泛关注。本文介绍了转基因食品的发展现状,并对转基因食品的检测技术和安全性评价进行了全面的综述,为进一步开展转基因食品的定性检测及其安全性评价工作提供一定参考。      

转基因食品的优点和安全性

尽管一些专家和消费者对转基因作物及食品持有保留态度,但是DNA重组技术的应用还是带来了许多好处,采用了这一技术的作物拥有了一些改良的品性:如抗病、抗旱以及抗虫,改良后的作物不仅产量和营养价值提高,而且外观和味道都会更好。本文对基因修饰食品及相应的DNA重组技术的优点和安全性问题作了详尽的介绍。     

烟草基因工程安全管理现状与安全性评价研究进展

以基因工程为主导的现代生物技术的崛起，使得生命科学成为当今自然科学研究的三大热点之一。基因工程研究的飞速发展，为现代农业生产开拓了新思维，但是事物总有其两面性。笔者就烟草基因工程安全管理现状，安全性评价研究所涉及到的基因转移的过程、基因漂移、病毒起源的基因和其它病毒的相互作用和重组、选择标记、毒性问题与新蛋白 产生、环境问题、转基因烟草是否可以转变成杂草等、管理上的挑战、公众的理解和未来展望等方面作了综述。