Fat structure in ice cream: A study on the types of fat interactions

This study investigated the type of fat interactions that lead to fat structuring in ice cream, and the effect of droplet shape on the interactions. These include fat crystal-induced partial coalescence, protein-induced (bridging) flocculation, coalescence or a combination of effects. The mechanisms by which SDS and EDTA dissociate fat aggregates could further our understanding of fat structuring. SDS reverses partially coalesced droplets while EDTA re-disperses flocculated ones. Ice cream formulations were designed to favor different kinds of aggregation. The ice creams were prepared with blends of solid (palm kernel oil) and liquid (high oleic sunflower oil) fat (0, 40, 80 or 100% solid fat at whipping temperature) in the presence of 0.15% unsaturated (UM) or saturated monoglyceride (SM). Treatment of ice cream with SDS at temperatures preserving the fat crystals revealed large decreases in particle size. This verified the existence of substantial amounts of partial coalescence as the fat structuring mechanism. The influence of EDTA was lacking, exposing the absence of flocculation in the fat structure. This was further evidenced by inhibited aggregation in the absence of monoglyceride. Some coalescence occurred, dominating in ice creams devoid of solid fat. Transmission electron microscopy images showed a unique type of shape for droplets adsorbed with unsaturated monoglyceride; these droplets partially coalesced to a larger extent than conventional SM-stabilized droplets.     

Proteolysis of milk fat globule membrane proteins during in vitro gastric digestion of milk

The influence of gastric proteolysis on the physicochemical characteristics of milk fat globules and the proteins of the milk fat globule membrane (MFGM) in raw milk and cream was examined in vitro in simulated gastric fluid (SGF) containing various pepsin concentrations at pH 1.6 for up to 2 h. Apparent flocculation of the milk fat globules occurred in raw milk samples incubated in SGF containing pepsin, but no coalescence was observed in either raw milk samples or cream samples. The changes in the particle size of the fat globules as a result of the flocculation were dependent on the pepsin concentration. Correspondingly, the physical characteristics of the fat globules and the composition of the MFGM proteins in raw milk changed during incubation in SGF containing pepsin. The major MFGM proteins were hydrolyzed at different rates by the pepsin in the SGF; butyrophilin was more resistant than xanthine oxidase, PAS 6, or PAS 7. Peptides with various molecular weights, which altered with the time of incubation and the pepsin concentration, were present at the surfaces of the fat globules.     

Effect of Solid Fat Content on Structure in Ice Creams Containing Palm Kernel Oil and High-Oleic Sunflower Oil

ABSTRACT: The development of a structural fat network in ice cream as influenced by the solid:liquid fat ratio at the time of freezing/whipping was investigated. The solid fat content was varied with blends of a hard fraction of palm kernel oil (PKO) and high-oleic sunflower oil ranging from 40% to 100% PKO. Fat globule size and adsorbed protein levels in mix and overrun, fat destabilization, meltdown resistance, and air bubble size in ice cream were measured. It was found that blends comprising 60% to 80% solid fat produced the highest rates of fat destabilization that could be described as partial coalescence (as opposed to coalescence), lowest rates of meltdown, and smallest air bubble sizes. Lower levels of solid fat produced fat destabilization that was better characterized as coalescence, leading to loss of structural integrity, whereas higher levels of solid fat led to lower levels of fat network formation and thus also to reduced structural integrity. Practical Application: Blends of highly saturated palm kernel oil and monounsaturated high-oleic sunflower oil were used to modify the solid:liquid ratio of fat blends used for ice cream manufacture. Blends that contained 60% to 80% solid fat at freezing/whipping temperatures produced optimal structures leading to low rates of meltdown. This provides a useful reference for manufacturers to help in the selection of appropriate fat blends for nondairy-fat ice cream.     

Oxidation and textural characteristics of butter and ice cream with modified fatty acid profiles

The primary objective of this study was to evaluate oxidation and firmness of butter and ice cream made with modified milkfat containing enhanced amounts of linoleic acid or oleic acid. The influence of the fatty acid profile of the HO milkfat relating to product properties as compared with the influence the fatty acid profile of the HL milkfat was the main focus of the research. Altering the degree of unsaturation in milkfat may affect melting characteristics and oxidation rates, leading to quality issues in dairy products. Three milkfat compositions (high-oleic, high-linoleic, and control) were obtained by modifying the diets of Holstein cows. Ice cream and butter were processed from milkfat obtained from cows in each dietary group. Butter and ice cream samples were analyzed to determine fatty acid profile and firmness. High-oleic milkfat resulted in a softer butter. Solid fat index of high-oleic and high-linoleic milkfat was lower than the control. Control ice cream mix had higher viscosity compared with high-oleic and high-linoleic, but firmness of all ice creams was similar when measured between -17 and -13 degrees C. Nutritional and textural properties of butter and ice cream can be improved by modifying the diets of cows.     

大豆蛋白与酪蛋白比例对搅打稀奶油稳定性影响的研究

研究了大豆蛋白与酪蛋白不同配比对搅打稀奶油乳浊液的表观粘度及搅打过程中的液相蛋白浓度、脂肪部分聚结、搅打起泡率的变化和泡沫稳定性的影响,并在此基础上探讨了其作用机理。结果表明:大豆蛋白比例的增大能增加界面膜的粘弹性,抑制脂肪球的部分聚结,提高泡沫结构的稳定性,当大豆蛋白与酪蛋白比例为4∶1时,搅打稀奶油可以获得最佳的稳定性。     

The fat globule membrane in ice cream

A method for determining the average thickness of the membrane around the fat globules in homogenised ice cream mix from the mix viscosity is examined. The difficulties with this approach, such as the non-Newtonian behaviour of the mix, the polydisperse nature of the fat globule size distribution and the contribution of the micellar casein to the volume fraction of the aqueous phase, are discussed. Values of 60 to 90 nm for the effective “hydrodynamic” thickness have been obtained for ice cream mixes of 9% w/w vegetable fat and 10.5% w/w skim milk solids. It is suggested from these values, and from the interpretation of electron micrographs of similar mixes, that part of the micellar casein is attached to fat globules, thus increasing the effective volume fraction of the fat and accounting for the abnormally high viscosities of these mixes.     

油脂用量对搅打稀奶油的搅打性能和品质的影响

研究了油脂用量对搅打稀奶油的粒度分布、脂肪部分聚结、液相蛋白质浓度、搅打起泡率、质构特性、感官品质和稳定时间的影响。研究表明:随着油脂用量增加,冷却后乳浊液脂肪球粒径增大;搅打过程中脂肪部分聚结速度和脂肪球粒径d4,3均随油脂用量增加而增大,且脂肪部分聚结率与脂肪球粒径d4,3有很好的相关性;液相蛋白质浓度和搅打起泡率降低;搅打稀奶油的质构特性值增加;稳定时间呈先增后减趋势,当油脂用量为23%时,搅打稀奶油的稳定时间最长达到2.7h;搅打稀奶油的感官品质以油脂用量为20%最好,综合考虑,油脂最佳用量范围是20%-23%。     

脂肪球在搅打乳状液中的部分聚结及其作用

综述了脂肪球在搅打乳状液中的部分聚结现象和搅打条件、脂肪种类、脂肪球吸附膜对部分聚结的影响。以冰淇淋和搅打奶油为例 ,说明脂肪球的部分聚结对于搅打乳状液最终形成充气的泡沫结构起关键作用 ,控制脂肪球的部分聚结程度对于改善搅打乳制品的质量、性能具有重要意义。联系控制脂肪球的部分聚结在冰淇淋生产中的实际应用 ,介绍了近来国际上有关脂肪球部分聚结的研究进展。     

Influence of formulation on the structural networks in ice cream

Six different formulations, corresponding to three types of fat (hydrogenated or refined coconut oils or refined palm oil) and two mixtures of mono- and di-glycerides, namely saturated and partially unsaturated were chosen to investigate the influence of the oil phase nature and the low molecular weight emulsifier type on the networks present in ice cream. Ice creams were characterized for particle size distribution of fat globules, melting resistance and amount of proteins in the aqueous phase. Variation of rheological parameters as a function of temperature allowed following the ice network melting, the fat globule aggregation state and destabilization, and the structural arrangement of proteins. Presence of unsaturated fatty acids in the emulsifier promoted an increased percentage of agglomerated fat globules, increased melting time and higher storage modulus values at 5 °C. The influence of the fat type on ice cream characteristics was mainly illustrated by different rheological parameters and, to a lesser extent, by melting time, whereas the amount of proteins in the aqueous phase did not allow discriminating among the formulations.     

黄油-代可可脂基奶油生产工艺优化及其晶型形成分析

在分析油脂用量对黄油-代可可脂基奶油品质影响的基础上,通过正交试验研究油脂用量、黄油-代可可脂质量比和均质压力对奶油搅打性能、质构特性、脂肪球部分聚结率、结晶特性等的影响。结果表明,在油脂用量30%、黄油-代可可脂质量比2∶3、均质压力60?MPa的条件下,能保证脂肪部分聚结的发生,并保持搅打稀奶油较好的感官特性、起泡性和结晶特性。60?MPa的均质压力保证了体系的稳定性,使大量游离脂肪球产生从而促进脂肪部分聚结。晶型形成分析结果表明,在优化条件下,原料乳浊液X-射线衍射峰峰形由较宽、较尖锐变为峰形适中均匀,适宜搅打稀奶油的β’晶型晶体明显增多,搅打稀奶油乳浊液在低温条件下发生脂肪结晶,表现为脂肪晶体尺寸增大。     

COLLOIDAL STABILITY OF ICE CREAM MIX*

The contributions of oil globule size (d_v), emulsifying agent, hydrocolloid stabilizer, and temperature, to the stability of ice cream mix have been investigated. On ageing the mix at 20 ^°C, the oil globules coalesce in one or two stages, depending on the initial size. When this is less than approximately 0.95 n, coalescence proceeds rapidly until a critical size is reached. The number of oil globules per unit volume of mix (TV) and variations in holding temperature influence the rate of coalescence at this stage. Subsequent coalescence, which is unaffected by N, proceeds at a much slower rate. Mixes with initial globule sizes exceeding 0.95 μ show only this second stage. It is suggested that the sharp reduction in coalescence rate for globules of diameter greater than 0.95 μ arises from the pronounced increase in the energy barrier to coalescence. Decreasing inter-facial area with increasing diameter of the globules leads to a more rigid emulsifier layer at the oil-water interface. Milk protein is the main contributor to stability during slow coalescence. The emulsifier exerts only a synergistic effect. Data are provided to show how the texture of frozen ice cream may be related to the initial size of the globules in the mix from which it is prepared.     

The effect of inulin on the physicochemical properties and sensory attributes of low-fat ice cream

The aim of this study was to investigate the feasibility of replacing the ice cream fat with inulin to produce a low-fat ice cream with prebiotic properties. For this purpose, inulin (2, 3 and 4%, w/w) was added to the low-fat ice cream and the physicochemical and sensory properties of the resultant ice creams were compared with those of control ice cream (containing 10% fat) and the inulin-free low-fat ice cream. The composition, pH and acidity of the ice cream mixes were measured and the melting rate, colour and texture of the frozen ice cream samples were examined. The results indicated that the low-fat ice creams had a significantly lower melting rate in comparison with the control. Inulin addition caused the adhesiveness and hardness of the low-fat ice creams to decrease significantly compared with inulin-free low-fat ice cream.     

瓜尔豆胶对搅打稀奶油的搅打性能的影响

研究了不同浓度的瓜尔豆胶对搅打稀奶油乳状液的表观黏度、脂肪球粒度、脂肪球界面蛋白浓度、脂肪球部分聚结率、泡沫硬度和搅打起泡率的影响。结果表明，瓜尔豆胶对搅打稀奶油乳状液的表观黏度影响非常显著；瓜尔豆胶浓度过高或过低，都会使得解冻后的乳状液粒径变大；瓜尔豆胶的质量分数越高，脂肪球部分聚结速度越快，泡沫硬度也越大；搅打起泡率随着瓜尔豆胶质量分数增大而降低。     

Enrichment of Functional Properties of Ice Cream with Pomegranate By‐products

Pomegranate peel rich in phenolics, and pomegranate seed which contain a conjugated fatty acid namely punicic acid in lipid fraction remain as by‐products after processing the fruit into juice. Ice cream is poor in polyunsaturated fatty acids and phenolics, therefore, this study was conducted to improve the functional properties of ice cream by incorporating pomegranate peel phenolics and pomegranate seed oil. Incorporation of the peel phenolics into ice cream at the levels of 0.1% and 0.4% (w/w) resulted in significant changes in the pH, total acidity, and color of the samples. The most prominent outcomes of phenolic incorporation were sharp improvements in antioxidant and antidiabetic activities as well as the phenolic content of ice creams. Replacement of pomegranate seed oil by milk fat at the levels of 2.0% and 4.0% (w/w) increased the conjugated fatty acid content. However, perception of oxidized flavor increased with the additional seed oil. When one considers the functional and nutritional improvements in the enrichment of the ice cream together with overall acceptability results of the sensory analysis, then it follows from this study that ice creams enriched with pomegranate peel phenolics up to 0.4% (w/w) and pomegranate seed oil up to 2.0% (w/w) could be introduced to markets as functional ice cream. Enrichment of ice creams with pomegranate by‐products might provide consumers health benefits with striking functional properties of punicalagins in pomegranate peel, and punicic acid in pomegranate seed oil.     

脂肪替代物在低脂冰淇淋中的应用研究进展（网络首发、推荐阅读）

冰淇淋作为一种复杂的多相食品,由分散在半冷冻溶液中的脂肪球、气泡和冰晶组成。其脂肪含量通常为8%~16%,在冰淇淋体系中起着重要作用。然而,脂肪摄入过多会对健康造成不利影响,因此采用脂肪替代物部分或全部代替冰淇淋中的脂肪成为研究热点。综述了近年来国内外学者对于冰淇淋中脂肪替代物的研究进展,侧重于不同类型脂肪替代物的介绍,以及脂肪替代物对低脂冰淇淋理化和感官特性的影响,总结了其应用情况,并对未来的研发方向和重点进行了展望。     

Effect of proteose-peptone addition on some physico-chemical characteristics of recombined dairy creams

The effect of the addition of the total proteose-peptone (TPP) fraction on some physico-chemical properties of recombined cream was studied. Oil-in-water emulsions, 30% or 20% (w/w) fat, were prepared using only the dairy components buttermilk, milkfat and TPP. The effect of different concentrations of TPP on droplet size, creaming stability, flow behaviour, viscosity and whippability of recombined creams was tested. Of the different creams, those containing 2% (w/w) or more TPP were more viscous, showed different flow behaviour, and had improved stability and whippability compared with the other creams. The modifications in physico-chemical properties appeared to be driven by changes in particle size distribution caused by droplet aggregation. The percentage of fat also influenced the properties of the final product. It may therefore be possible to obtain desirable modifications in recombined cream using only dairy ingredients.     

Production and stabilization of a flaxseed oil multi-layer emulsion containing sodium caseinate and pectin

The purpose of this research is the evaluation of a flaxseed oil-in-water emulsion, stabilized by a multi-layer structure consisting of sodium caseinate (Na-caseinate) and pectin to provide a basis for the combination of these materials for future studies. In the first step, the o/w emulsion (10 g oil, 90 g aqueous phase, and a pH 6.8) with varying concentration of Na-caseinate was investigated. Second, the pectin solution (0.05–1.5 g/100 g solution) was added to the primary emulsions and the pH was adjusted to 3.0. The emulsions were characterized by mean particle size (dynamic light scattering and static light scattering techniques), ζ-potential, turbidity value, creaming index, and the visualization of the microstructure. A clear separation of the oil phase at low protein contents and destabilizing by depletion flocculation at high protein content were observed. Extensive droplet flocculation and coalescence were determined until the pectin concentration reached 0.5 g/100 g solution for the secondary emulsion. After 7 days of storage, a 1.5 g/100 g solution pectin content had good stability with a relatively small size distribution, high turbidity value, and no cream phase separation.     

Using lipase activity to probe milkfat globule membranes (MFGM) or interfaces

Measurement of lipoprotein lipase activity (as free fatty acid change or accumulation) is accepted as indicative of milkfat globule membrane (MFGM) disruption. However, measurement is confounded by variables unrelated to the MFGM, e.g., microbial quality. To resolve this, a modified approach to using lipase activity to probe MFGM was developed. Methanol (1%, w/w) and lipoprotein lipase (2%, w/w, raw milk) were added to pasteurised milk samples resulting in formation of methyl esters that therefore served as a new and unique signal for lipase gaining access to interfacial fat. Analysis conditions minimised product formation to minimise sample perturbation. The method could detect 1% (w/w) of homogenised milkfat globules blended with native globules, with linear response to 10% (w/w) and saturation above 20% (w/w). The approach was applied to assess MFGM in samples subjected to disruptive treatments (shear, aeration) under different conditions; findings were consistent with previous research, but with greater sensitivity.     

葵花籽油体富集物添加量对低脂冰淇淋浆料及产品品质的影响

为了探究葵花籽油体富集物在冰淇淋中应用的可行性,以葵花籽油体富集物作为脂肪来源制备低脂冰淇淋,在无均质条件下研究葵花籽油体富集物添加量对低脂冰淇淋浆料和产品品质的影响。结果表明：冰淇淋浆料的黏度、储能模量和损耗模量都随葵花籽油体富集物添加量的增加而增大,当葵花籽油体富集物添加量为3.5%～4.0%时黏度与对照组相当;浆料中不易流动水和结合水的比例随着葵花籽油体富集物添加量的增加逐渐增大,在4.0%时达到最大;冰淇淋的膨胀率和抗融率随着葵花籽油体富集物添加量的增加先增大后减小;添加葵花籽油体富集物的冰淇淋色泽较差;冰淇淋的硬度和咀嚼性变化规律一致,都是随着葵花籽油体富集物添加量的增大呈现先减小后增大的趋势,弹性变化则相反;冰淇淋黏附性随着葵花籽油体富集物添加量的增加而升高;葵花籽油体富集物添加量为2.5%时冰淇淋感官评分最高。合适的葵花籽油体富集物添加量可以起到与棕榈油相似的效果,因此葵花籽油体富集物可以应用在冰淇淋中。     

Influence of hydrolysed lecithin addition on protein adsorption and heat stability of a sterilised coffee cream simulant

Within an overall goal to enhance the stability of coffee cream, the influence of hydrolysed soybean lecithin addition was investigated. To avoid the seasonal variations in milk composition a model system was used containing 5% (w/w) soybean oil and 12% (w/w) skimmed milk powder with and without lecithin addition before a two-step high-pressure homogenisation. Addition of 20% (w/v) caesium chloride enabled a nearly complete recovery of the fat in the cream layer upon centrifugation of casein-stabilised emulsions. From the fat and protein content of this cream layer, it was concluded that lecithin addition did not significantly affect the protein content of the cream layer after emulsion preparation, but significantly reduced the additional milk protein adsorption at the oil/water interface upon sterilisation. The experimental data suggest that the enhanced heat stability of the lecithin-supplemented coffee cream simulant may be explained by the fact that hydrolysed lecithin largely reduces attractive protein–protein interactions during sterilisation.