共查询到20条相似文献,搜索用时 515 毫秒
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以人参为原料,研究人参多糖分离纯化方法得到均一多糖,并对其相对分子质量、单糖组成、红外光谱(Infrared,IR)进行分析。利用三氯乙酸法(Trichloroacetic Acid,TCA)除蛋白,再经DEAE-Cellulose-52柱和HW-55F凝胶柱分离纯化获得均一组分PGS1-1。采用凝胶柱层析法测定其相对分子质量,高效液相色谱与蒸发光散射检测器联用及IR研究PGS1-1的单糖组成及基本结构。研究表明三氯乙酸除蛋白最佳工艺条件为料液比1∶2,三氯乙酸(TCA)浓度12%,作用时间60 min,此条件下测得蛋白脱除率与多糖保留率综合得分87.80。分离纯化得到PGS1-1组分,其相对分子质量为2.8×104Da,主要由葡萄糖、半乳糖、鼠李糖、阿拉伯糖组成,物质的量比为0.34∶0.27∶1.44∶0.42,IR证明PGS1-1具有吡喃型β-糖苷键的多糖特征峰,为人参多糖的分离纯化及单糖组成提供了理论依据。 相似文献
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以鹿茸中下段胶原蛋白为酶解底物,用木瓜蛋白酶酶解制备小分子抗氧化肽,以清除1,1-二苯基-2-苦基肼基(DPPH?)自由基的能力为指标,采用响应面法优化酶水解条件。结果表明,最优实验条件为时间56 min,酶添加量1.40wt%,pH=5.60,温度60℃。该条件下所得抗氧化肽对DPPH?自由基的清除率为83.09%。用超滤膜、半制备色谱柱和超高效液相色谱?质谱联用仪分级分离获得分子量0.2?0.6 kDa的具有最高抗氧化活性的多肽,其具有与头段类似的保健功效,更易被人体吸收,且易进一步加工和储存。 相似文献
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选取紫花苜蓿根为研究对象,利用热水回流法提取紫花苜蓿根多糖,采用Sevage法和酶法联合去蛋白,脱色后采用DEAE纤维柱和凝胶柱层析分离得到纯化苜蓿根多糖(DAPS-1)。采用高效液相凝胶色谱测定DAPS-1的相对分子质量为26.95 kDa,通过红外光谱对多糖的结构表征,结果显示DAPS-1是一类α-吡喃型糖。体外清除自由基实验表明DAPS-1是具有抗氧化活性多糖。以HepG2细胞为模型,考察了DAPS-1对油酸诱导的非酒精性脂肪肝细胞模型的干预作用,结果表明DAPS-1可显著降低油酸诱导的HepG2细胞内脂质的累计,具备一定的降脂效果。 相似文献
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文章介绍夏枯草中降糖成分提取条件的优化研究,以熊果酸为指标物。采用乙醇加热回流法,检测方法采用紫外分光光度法,检测波长为206nm。用石油醚洗涤去除大部分杂质。选择乙醇浓度,料液比,提取温度,提取时间为考察因素。确定夏枯草中熊果酸最佳工艺条件为80%乙醇为溶剂,料液比1︰20,在80℃恒温下,水浴回流110min。该方法简单易行。 相似文献
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目的优化伤寒Vi多糖柱层析纯化工艺,以替代传统苯酚抽提、乙醇分级沉淀法。方法采用DEAE离子交换层析柱,分别在缓冲液(20 mmol/L Tris-Cl)pH值为6.0、7.0和8.0的条件下纯化1批伤寒Vi多糖粗糖,并与传统苯酚抽提、乙醇分级沉淀法进行比较。按《中国药典》三部(2010版)要求检测纯化样品的蛋白质含量、核酸含量、O-乙酰基含量、分子大小及内毒素含量,并计算样品的多糖回收率。采用优化的DEAE柱层析纯化工艺纯化3批伤寒Vi多糖粗糖,验证该工艺的稳定性。结果 DEAE离子交换柱缓冲液(20 mmol/L Tris-Cl)pH值为7.0和8.0时,可有效将伤寒Vi多糖的杂质与纯化产物分离,多糖回收率分别为36.52%和38.35%,明显高于传统工艺的多糖回收率(15.52%),且纯化产物的内毒素含量(10 EU/μg)明显低于传统工艺(200 EU/μg)。以优化工艺(pH 7.0)纯化3批伤寒Vi多糖粗糖获得的6批精糖的蛋白质含量、核酸含量、O-乙酰基含量、分子大小、内毒素含量及多糖回收率差异较小,标准偏差均小于5%。结论优化了伤寒Vi多糖柱层析的纯化工艺,该工艺稳定性良好,可替代传统苯酚抽提、乙醇分级沉淀法,应用于伤寒Vi多糖的纯化。 相似文献
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Yaping Li Linshang Zhang Zhide Wang Yanlan Bi Dan Peng 《Journal of the American Oil Chemists' Society》2022,99(4):331-340
Natural standard grade γ-tocopherol is of great interest due to its good antioxidant activity and special pharmacological functions. This study separated and purified γ-tocopherol from mixed tocopherols using eco-friendly and low toxicity solvents. γ-Tocopherol (0.28 g) was obtained per performance with a purity of (98.89 ± 0.68)% and a recovery rate of (93.23 ± 0.89)%. The purification conditions were as follows: elution solvent n-hexane/ethyl acetate 94.5:5.5 (v/v), sample load 0.5 g, a column height to diameter ratio of 16:1 (length and diameter, 60 × 3 cm) and an elution rate of 2 ml/min. The purity and structure of γ-tocopherol was confirmed by high-performance liquid chromatography, gas chromatography/mass spectrometry and nuclear magnetic resonance. The adsorption isotherm of γ-tocopherol on silica gel (200–300 mesh) fitted the Langmuir isotherm model well. The antioxidant activity analysis showed that γ-tocopherol had the strongest antioxidant activity followed by δ-tocopherol and α-tocopherol. This study provides an economically attractive solution for the production of natural standard grade γ-tocopherol. 相似文献
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Yijun Fan Qinyi He Aoshuang Luo Miaoyu Wang Aoxue Luo 《International journal of molecular sciences》2015,16(3):6391-6401
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus. 相似文献
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A hybrid chromatography-crystallization process to purify wedelolactone obtained from Eclipta alba was developed. The crude extract was obtained by microwave-assistant extraction and purified by silica gel column chromatography in which dichloromethane–methanol–acetic acid solution was used as the eluent. Dilution crystallization was employed to perform the final purification. To optimize the processing parameters, single factor analysis and response surface methodology were employed. The results indicated that the purification yield and purity of wedelolactone could reach 77.66% and 99.46%, respectively. Antioxidant assays showed that the products had strong antioxidant and free radical scavenging activity which was close to oligomeric proantho cyanidins. 相似文献
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Porphyra haitanensis polysaccharide was separated and purified by microwave-assisted extraction with sequential DE-52 anion-exchange chromatography and Sephadex G-100 size-exclusion chromatography. The maximal yield of polysaccharide was 3.6% by microwave-assisted extraction. After purification, three polysaccharide components (PY1, PY2, and PY3) were obtained. PY1 had α-glycosidic bonds, whereas PY2 and PY3 had the β-amide pyranose. Sulfate groups and 3,6-anhydrogalactose were presented in three purified polysaccharide components. PY1 contained galactose and a little of xylose and arabinose. PY2 contained galactose and a little of arabinose, xylose, glucose, and mannose. The contents of sulfate groups, 3,6-anhydrogalactose, and galactose were also determined. 相似文献