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在SIP应用服务器中,由于大量用户及各种不同业务呼叫的存在,在用户检索、数据存储的速度及实时计费等方面对数据库的性能要求高,内存数据库的特性可以满足这种需求,本文介绍在SIP应用服务器中内存数据库的一种设计思路及实现方法,并对该内存数据库提供的接口进行说明;该内存数据库已经在实际的产品中使用,其可靠性及性能已经得到验证。 相似文献
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IMS(Information Management System)是一种层次型的数据库管理系统,其数据以一种树型的逻辑拓扑结构进行存储,非常适合支持高可用性、高性能、高容量、低成本的关键性联机应用程序.对IMS系统进行了抽象地分析,介绍了IMS系统的优势,建立了IMS系统的模型结构,并深入分析了IMS的可恢复性以及系统恢复机制. 相似文献
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José Luis Sierra Pablo Moreno‐Ger Iván Martínez‐Ortiz Baltasar Fernández‐Manjón 《Software》2007,37(4):441-461
The <e‐Aula> platform is a new experimental e‐learning environment that adheres closely to IMS Global Learning Consortium, Inc. e‐learning standards in order to facilitate their applicability in different learning scenarios. <e‐Aula> is equipped with an integrated modular and extensible architecture for the authoring of IMS‐compliant learning materials focused on the IMS manifest. This manifest‐driven architecture facilitates maintenance and promotes the evolution of the authoring system in <e‐Aula>, both of which are mandatory requirements in the successful production and maintenance of content for many different specialized learning domains. In this paper, we describe this authoring system, its manifest‐driven architecture and its implementation using well‐known and robust Java‐based Web technologies. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
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Daniel Baechle Katrin Sparbier Hassan Dihazi Sabine Blaschke Gerhard‐Anton Mueller Markus Kostrzewa Thomas Flad Dr. 《Proteomics. Clinical applications》2007,1(10):1280-1284
In routine clinical diagnostics, peptide biomarkers are most commonly quantified using immunological techniques but these methods often lack sensitivity and/or specificity. Hence, quantitative mass spectrometry detection is desirable as an alternative diagnostic tool. To date, quantitative mass spectrometry is mostly based on ESI‐MS coupled to LC, requiring highly sophisticated instrumentation and knowledge and is time consuming and expensive. In contrast, MALDI‐TOF‐MS is a very simple, sensitive and rapid method for the detection of peptide biomarkers. However, the infeasibility of absolute quantification has been a tremendous handicap to the use of MS in stable clinical diagnostics. Here, we describe the development of a technical platform based on ClinProt particles and heavy‐isotope internal peptide standards for the fast and reliable preparation of samples. This combines the advantages of MALDI‐TOF as a read‐out system with absolute quantitation of peptide biomarkers. As a proof‐of‐concept, this platform was successfully employed for the absolute determination of the concentration of the highly abundant serum peptide des‐Ala‐Fibrinopeptide A in 45 serum samples from healthy donors. Such technology essentially contributes to the development of a stable MALDI‐TOF‐MS‐based clinical assay. 相似文献
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Jimenez CR El Filali Z Knol JC Hoekman K Kruyt FA Giaccone G Smit AB Li KW 《Proteomics. Clinical applications》2007,1(6):598-604
Serum peptide profiling by MS is an emerging approach for disease diagnosis and biomarker discovery. A magnetic bead‐based method for off‐line serum peptide capture coupled to MALDI‐TOF‐MS has been recently introduced. However, the reagents are not available to the general scientific community. Here, we developed a protocol for serum peptide capture using novel magnetic C18 beads, and automated the procedure on a high‐throughput magnetic particle processor. We investigated bead equilibration, peptide binding and peptide elution conditions. The method is evaluated in terms of peaks counts and reproducibility of ion intensities in control serum. Overall, the DynaBead‐RPC18‐based serum sample processing protocol reported here is reproducible, robust and allows for the detection of ?200 peptides at m/z 800–4000 of serum that was allowed to clot for 1 h. The average intra‐experiment %CV of normalized ion intensities for crude serum and 0.5% TFA/0.15% n‐octyl glucoside‐treated serum, respectively, were 12% (range 2–38%) and 10% (3–21%) and the inter‐experiment %CVs were 24% (10–53%) and 31% (10–59%). Importantly, this method can be used for serum peptide profiling by anyone in possession of a MALDI‐TOF instrument. In conjunction with the KingFisher® 96, the whole serum peptide capture procedure is high‐throughput (?20 min per isolation of 96 samples in parallel), thereby facilitating large‐scale disease profiling studies. 相似文献
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Giulia Bernardini Maurizio Comanducci Stefania Bambini Giovanni Renzone Andrea Scaloni Giovanna Morelli Mark Achtman Giulio Ratti Annalisa Santucci 《Proteomics. Clinical applications》2009,3(10):1251-1254
We previously described the first reference map for the proteome of one strain of serogroup A Neisseria meningitidis (MenA), a major cause of epidemic meningitis in humans. As a preliminary finding, in that work we noted that 2‐DE protein maps of closely related MenA isolates from different epidemics spreads could be easily compared to detect minor differences and that 2‐DE phenotypes attributable to the well‐known epidemiological marker tbpB agreed with the genoclouds model of MenA epidemiological variation during pandemic waves. We explored here the possibility that an extended comparative study of 2‐DE maps of isolates representative of the nine genoclouds described by Achtman and collaborators could be used to discriminate between strains otherwise undistinguishable. We showed the example of 14 proteins with different 2‐DE spot patterns in different genoclouds that could be considered as putative tracers for alike‐strains discrimination. We introduce the novel concept that comparative proteomics can be useful in identifying new epidemiological markers for N. meningitidis. 相似文献
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本文在分析和研究WebRTC技术的基础上,提出一种架构,在电信运营商原有的IMS视频会议系统上增加对WebRTC客户端(浏览器客户端)的支持。除传统终端外,系统可使用浏览器(无需安装任何插件)进行实时语音/视频通信,增强了易用性和实用性,为同类视频业务的开发提供了借鉴。文中最后提出了该系统有待优化的地方,并展望了WebRTC技术在推动运营商IMS视频业务发展方面的前景。 相似文献
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针对IMS(IP Multimedia Subsystem)中已注册用户的重注册过程,提出了一种改进的快速重注册方法.由于已注册用户的UE(User Equipment)已经记录归属地S-CSCF(Serving-Call Session Control Function)的地址信息,因此在重注册过程中,通过在REGISTER消息中携带S-CSCF的路由信息,让REGISTER消息直接从拜访地的P-CSCF(Proxy-Call Session Control Function)转发到归属地的S-CSCF,可以有效地减少重注册延时.分析结果显示,改进的快速重注册过程要优于标准重注册过程和已有的一些改进过程.同时,它对网络的改动较小,容易实现. 相似文献
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《Proteomics. Clinical applications》2018,12(3)
Purpose
Human serum and plasma are often used as clinical specimens in proteomics analyses, and peptidome profiling of human serum is a promising tool for identifying novel disease‐associated biomarkers. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is widely used for peptidomic biomarker discovery. Careful sample collection and handling are required as either can have a profound impact on serum peptidome patterns, yet the effects of preanalytical variables on serum peptidome profiles have not been completely elucidated. The present study investigated the effects of preanalytical variables, including storage temperature, duration (up to 12 months), and thawing methods, on MALDI‐TOF MS‐based serum peptidome patterns.Experimental design
Aliquots of serum samples were pretreated with weak cation exchanger magnetic beads using an automated ClinProtRobot system and then analyzed by MALDI‐TOF MS.Results
A number of significant differences in peak intensities were observed depending on sample processing variables.Conclusions and clinical relevance
These peaks can be used as sample quality markers to assess the effects of long‐term storage on serum peptidome profiles using MALDI‐TOF MS.16.
目前我国轨道交通事业迅猛发展,各城市的地铁线路都分别建成了轨道交通综合监控系统,但是,一条线路的地铁综合监控系统只能监控本条地铁线路系统内的信息,为了突破此一监控现状的局限性,将各条地铁线路的信息综合至同一个后台进行信息收集、监视控制已成为当前轨道交通行业内的最新最先进的运营发展模式,本文描述了一种轨道交通综合监控系统(IMS)与自动售检票(AFC)专业特殊接口协议方案,通过IMS与ACC的信息交互,使得可以在一个统一的监控平台监视南京地铁1号线、南京地铁1号线南延线以及南京地铁2号线全部的自动售检票系统综合信息。 相似文献
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张蓓蕾 《数字社区&智能家居》2009,5(10):8014-8016
在分析现有网络教学平台中练习与测试系统的不足基础上,对练习与测试互操作规范QTI（Question＆Test Interoperability Specification）进行了研究。探讨了QTI规范的目标和组成,分析了常用题型的存储结构特点,并借助QTI规范实现了题目的标准化存储,节省了数据库资源,方便了教师之间共享题库资源。 相似文献
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Acosta-Martin AE Chwastyniak M Beseme O Drobecq H Amouyel P Pinet F 《Proteomics. Clinical applications》2009,3(10):1236-1246
The aim of our study was to analyze the proteomic pattern of human macrophages obtained over a 4 year period from blood donors. The purpose was to simulate a long-term clinical study to assess the application of 2-D DIGE technique for differential proteomic analysis of these scarce samples. Bioinformatic analysis of 2-D DIGE gels of 19 different cultures of macrophages assessed whether they did or did not contain at least specific five spots identified by MS as being or containing bovine deoxyribonuclease I (DNase I). Bovine DNase I was used during sample treatment to remove nucleic acids from protein extracts. Macrophages were classified in two groups, which appeared to be differentiated by the completeness of DNase I treatment. Further detailed analysis revealed a different proteomic pattern of macrophage protein samples according to the completeness of this treatment. The major group of proteins affected, accounting for one third of the differentially expressed proteins, included proteins involved in cell motion and actin cytoskeleton reorganization. The use of DNase I for the removal of nucleic acids from protein samples must be avoided in proteomic studies since it can generate bias in the analysis of protein expression patterns. 相似文献
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Darbepoietin (DAR) and recombinant human erythropoietin (rhEPO) stimulate erythropoiesis, leading to an increase in red blood cells. Along with their legitimate clinical use, rhEPO and DAR are also misused in racing horses for performance enhancement. To control the illegal use of DAR and rhEPO, it is important to develop analytical methods for the detection and confirmation of these proteins in plasma. Analysis of rhEPO and DAR in plasma is challenging due to the presence of a number of high abundance proteins including albumin that interferes with their extraction. The present study showed that the extraction of rhEPO or DAR from plasma using anti-EPO-antibody coupled immunoaffinity (IA) extraction yielded low (25-40%) recovery. Albumin-depletion using antialbumin-antibody coupled IA columns also depleted the target proteins and further reduced their recovery. Pre-extraction of spiked plasma using hydroxyapatite (HTP)-ProGel or ConA columns followed by the IA column yielded 65 to 70% recovery. The extracted samples were (i) analyzed directly with or without SDS-PAGE for intact proteins and (ii) analyzed after trypsin hydrolysis, with or without SDS-PAGE, for peptide fingerprinting using MALDI-TOF. Trypsin and enolase were used as internal calibrators for intact protein analysis and a peptide EYEATLEECCAK was used as internal calibrator for fragment analysis. Analysis of extracted sample without SDS-PAGE yielded, along with the target proteins (rhEPO and DAR), albumin and other related proteins. SDS-PAGE separated the target proteins with albumin and yielded clean samples. Inclusion of internal calibrators resulted in a linear dose-response relationship for both intact protein and digested fragments and allowed quantification of the target peptides. Thus, extraction of plasma using a combination of ConA and IA extractions yielded approximately 70% recovery of target proteins with a small amount of albumin and other proteins. SDS-PAGE improved the quality of the MALDI-TOF results. Minimum detection limits for digested fragments were lower than those for intact proteins. 相似文献