A highly sensitive and selective colorimetric and off-on fluorescent chemosensor for Cu based on rhodamine B derivative

A new rhodamine B derivative colorimetric and fluorescent sensor (1) was synthesized by condensation reaction of rhodamine B hydrazide and 2,4-dihydroxybenzaldehyde, which showed reversible and highly selective and sensitive recognition toward Cu²⁺ over other examined metal ions. Upon addition of Cu²⁺, sensor 1 exhibits remarkably enhanced absorbance intensity and color change from colorless to pink in DMSO and MeCN aqueous buffer solution or pure MeCN, and shows significant off-on fluorescence accompanied by color changes from colorless to orange in MeCN. The sensor 1 was also successfully applied to the determination of Cu²⁺ in water samples.     

Fe-selective fluorescent probe based on rhodamine B and its application in bioimaging

A Rhodamine-based fluorescent and colorimetric chemosensor for Fe³⁺ ion, acetyl rhodamine-hydroxamate (ARH), was designed and synthesized. Upon mixed with Fe³⁺ in CH₃CN-H₂O (1:1, v/v), the spirolactam of ARH was opened, which resulted in the dramatic enhancement of both fluorescence and absorbance intensity as well as the color change of the solution. Background metal ions showed small or no interference with the detection of Fe³⁺. The Job's plot indicated the formation of 1:1 complex between ARH and Fe³⁺. Confocal laser scanning microscopy experiments showed that ARH could be used to detect Fe³⁺ in living cells.     

Large nanoparticle-induced enhancement of recognition selectivity for Cu ion

A new fluorescent excited state intramolecular proton transfer compound, 2-(2-hydroxy-4-carbaldehydephenyl)benzoxazole (HCPBO), has been synthesized for the detection of Cu²⁺, based on its fluorescence quenching. In molecular monodispersed solution, the recognition selectivity for Cu²⁺ was poor as Co²⁺ and Ni²⁺ gave similar results in fluorescence quenching. However, the nanoparticles of HCPBO, prepared in ethanol-water (1:4, v/v), greatly enhanced the recognition selectivity for Cu²⁺. The mechanism was discussed as energy transfer (ET), energy migration (EM) and the more quantity of formed HCPBO-Cu²⁺ complex than other metal ions in the nanoparticle conditions enhanced the recognition selectivity for Cu²⁺ together.     

A new acridine derivative as a highly selective ‘off-on’ fluorescence chemosensor for Cd in aqueous media

A new acridine fluoroionophore containing two diethanolamine ligands, 4,5-bis(N,N-di (2-hydroxyethyl)iminomethyl)acridine (BHIA), was designed and synthesized based on the fluorophore-spacer-receptor format. And its fluorescent sensing behavior towards metal ions was investigated in buffered aqueous media. The presence of Cd²⁺ induces the formation of a 1:1 ligand/metal complex at neutral pH, which exhibits enhanced emission at 454 nm. The fluorescence intensity is linear with the Cd²⁺ concentration in the range of 1.0 × 10⁻⁶ to 3.0 × 10⁻⁵ M (R = 0.9967). Experimental results show a low interference response towards other metal ions. The selective switch-on fluorescence response of BHIA to Cd²⁺ makes it suitable for sensing of Cd²⁺ in aqueous solution. The detection limit is 1.3 × 10⁻⁷ M. Moreover, the results indicated that BHIA was a reversible chemosensor for Cd²⁺, which makes it attractive for sensing applications.     

Application of rhodamine B thiolactone to fluorescence imaging of Hg in Arabidopsis thaliana

Exposure to mercury causes severe damage to plants, animals and even humans. Concern over mercury toxicity has encouraged the development of efficient, sensitive, and selective methods for the in vivo detection of mercury. Although a variety of studies have been published describing fluorescence imaging of mercury in animal cells and tissues, no in vivo monitoring has been developed for plant systems until now. In this paper, we report the semi-quantitative fluorescence imaging of Hg²⁺ ions in a common model plant Arabidopsis thaliana (A. thaliana), with rhodamine B thiolactone (RBS) as a novel Hg²⁺ probe. The experimental results show that RBS is plant cell wall and cell membrane permeable, and the probe responds selectively to Hg²⁺ ions instead of the other species in plant systems. Real-time monitoring of Hg²⁺ absorption in roots of A. thaliana by RBS shows that saturation of Hg²⁺ uptake could occur in a short period of 3 days at most. The transportation and accumulation of Hg²⁺ ions in roots of A. thaliana have also been studied, revealing that most of Hg²⁺ ions reside in root cap and meristematic zone, and only a small amount of Hg²⁺ ions can reach the maturation zone. This indicates that the interaction of Hg²⁺ ions with any Hg²⁺-philic species including proteins in these regions may be responsible for plant poisoning and even death.     

深海适冷菌Pseudoalteromonas sp. SM9913胞外多糖对Pb2+和Cu2+的吸附性能研究

采用深海适冷菌Pseudoalteromonas sp. SM9913分泌的胞外多糖（EPS）分别对Pb²⁺和Cu²⁺进行吸附,研究了多糖用量、pH、吸附时间和共存离子对EPS吸附性能的影响及EPS对Pb²⁺和Cu²⁺的吸附热力学.结果表明,EPS对Pb²⁺和Cu²⁺的吸附量随EPS投加量的增加而减小.EPS对Pb²⁺和Cu²⁺的最佳吸附pH分别为4.5～5.5和4.5～6.0. EPS对Cu²⁺的吸附平衡时间为90　min,对Pb²⁺的吸附平衡时间则长达180　min.共存离子Ca²⁺、Mg²⁺、Na⁺、K⁺的加入均降低了EPS对Pb²⁺的吸附量,Ca²⁺、Mg²⁺的加入降低了EPS对Cu²⁺的吸附量,但低浓度的Na⁺和实验范围浓度的K⁺不仅没有降低反而增加了EPS对Cu²⁺的吸附量.Freundlich和Dubinin-Radushkevich方程均能较好地描述SM9913胞外多糖吸附Pb²⁺和Cu²⁺的热力学过程,由Dubinin-Radushkevich方程得到SM9913胞外多糖对Pb²⁺和Cu²⁺的最大吸附量分别为243.3 mg/g (10℃) 和36.7 mg/g (40℃).胞外多糖吸附金属离子前后的红外光谱分析表明,多聚糖中C—O—C、乙酰基和羟基是起主要吸附作用的官能团.     

Single quantum dot-micelles coated with gemini surfactant for selective recognition of a cation and an anion in aqueous solutions

The synthesis of quantum dot coated with cetyltrimethyl ammonium bromide (CTAB) and gemini surfactant [C₁₂H₂₅N⁺(CH₃)₂(CH₂)₄(CH₃)₂N⁺C₁₂H₂₅]·2Br⁻ (C_12-4-12) in aqueous solution have been described. It is characterized by photoluminescent spectroscopy, UV–vis spectroscopy and transmission electron microscopy (TEM), etc. In comparison with CTAB-coated QDs, the QDs coated with C_12-4-12 respond selectively to both transition metal ion copper and fluoride ion in aqueous media. When Cu²⁺ is bound to C_12-4-12-coated QD micelles, the fluorescence intensity is quenched. Linear relationships are found between the relative fluorescence intensity and the concentration of Cu²⁺ in the range 0–500 μM, which is best described by a Stern–Volmer-type equation. Meanwhile, it is found that F⁻ enhanced the luminescence of the C_12-4-12-coated QD micelles in a concentration dependence that is described by a Langmuir binding isotherm equation in the range 0–300 μM. The limits of detection of Cu²⁺ and F⁻ are 1.1 and 0.68 μM, respectively. The possible mechanism is discussed.     

Synthesis of Bi and Gd doped ZnB2O4 for evaluation as potential materials in luminescent display applications

A series of Bi³⁺ and Gd³⁺ doped ZnB₂O₄ phosphors were synthesized with solid state reaction technique. X-ray diffraction technique was employed to study the structure of prepared samples. Excitation and emission spectra were recorded to investigate the luminescence properties of phosphors. The doping of Bi³⁺ or Gd³⁺ with a small amount (no more than 3 mol%) does not change the structure of prepared samples remarkably. Bi³⁺ in ZnB₂O₄ can emit intense broad-band purplish blue light peaking at 428 nm under the excitation of a broad-band peaking at 329 nm. The optimal doping concentration of Bi³⁺ is experimentally ascertained to be 0.5 mol%. The decay time of Bi³⁺ in ZnB₂O₄ changes from 0.88 to 1.69 ms. Gd³⁺ in ZnB₂O₄ can be excited with 254 nm ultraviolet light and yield intense 312 nm emission. The optimal doping concentration of Gd³⁺ is experimentally ascertained to be 5 mol%. The decay time of Gd³⁺ in ZnB₂O₄ changes from 0.42 to 1.36 ms.     

Three-input chemical logic circuits based on a new fluorescent sensor for ATP and Cuin aqueous solution

A new l-lysine derivative featuring an anthracene unit is synthesized and characterized by elemental analysis, ESI-MS, ¹H-NMR, and ¹³C-NMR. It can selectively bind ATP in acidic aqueous solutions, and be used as a highly selective fluorescent chemosensor for Cu²⁺ at neutral pH, resulting in fluorescence quenching. According to these characters, two combinational logic circuits are fabricated on a single molecule with three chemical inputs [H⁺(IN1), OH⁻(IN2), ATP (IN31)] and [H⁺(IN1), OH⁻(IN2), Cu²⁺(IN32)], respectively.     

State-based versus event-based specifications for information systems: a comparison of B and eb 3

This paper compares two formal methods, B and eb³, for specifying information systems. These two methods are chosen as examples of the state-based paradigm and the event-based paradigm, respectively. The paper considers four viewpoints: functional behavior expression, validation, verification, and evolution. Issues in expressing event ordering constraints, data integrity constraints, and modularity are thereby considered. A simple case study is used to illustrate the comparison, namely, a library management system. Two equivalent specifications are presented using each method. The paper concludes that B and eb³ are complementary. The former is better at expressing complex ordering and static data integrity constraints, whereas the latter provides a simpler, modular, explicit representation of dynamic constraints that are closer to the user’s point of view, while providing loosely coupled definitions of data attributes. The generality of these results from the state-based paradigm and the event-based paradigm perspective are discussed.     

3D C-string: a new spatio-temporal knowledge representation for video database systems

In video database systems, one of the most important methods for discriminating the videos is by using the objects and the perception of spatial and temporal relations that exist between objects in the desired videos. In this paper, we propose a new spatio-temporal knowledge representation called 3D C-string. The knowledge structure of 3D C-string, extended from the 2D C⁺-string, uses the projections of objects to represent spatial and temporal relations between the objects in a video. Moreover, it can keep track of the motions and size changes of the objects in a video. The string generation and video reconstruction algorithms for the 3D C-string representation of video objects are also developed. By introducing the concept of the template objects and nearest former objects, the string generated by the string generation algorithm is unique for a given video and the video reconstructed from a given 3D C-string is unique too. This approach can provide us an easy and efficient way to retrieve, visualize and manipulate video objects in video database systems. Finally, some experiments are performed to show the performance of the proposed algorithms.     

Some quadrature formulas for functions partially holomorphic with respect to each variable in a Jordan polydomain in C 2 and a complex double integral formula

In this paper, quadrature formulas with remaining term in integral form for functions partially holomorphic with respect to each variable in a Jordan polydomain in C ² and a complex double integral formula are given. For this, we used the Cauchy Integral formula for Jordan polydomain in C ².     

Anti-windup compensation for systems with sensor saturation: a study of architecture and structure

This article proposes a linear dynamic anti-windup strategy for the alleviation of performance and stability problems in systems which are linear apart from saturating sensors. Unlike anti-windup compensation for systems subject to actuator saturation, there is no agreed architecture for applying anti-windup to systems with sensor saturation and therefore attention is devoted to the discussion of various candidate configurations which can be interpreted as a particular choice of a static non-linear map. The main results of the article give existence conditions for compensators which yield global exponential stability and finite ?₂ gain of the overall non-linear closed-loop system. The existence conditions are different to those which have appeared hitherto in the literature.     

Electrochemical DNA biosensor for the detection of interaction between di[azino-di(5,6-azafluorene)-κ-NN′]dichlormanganous and DNA

A new Mn(II) complex of MnL₂Cl₂ (L = azino-di(5,6-azafluorene)-κ²-NN′) was synthesized and utilized as an electrochemical indicator for the determination of hepatitis B virus (HBV) based on its interaction with MnL₂Cl₂. The electrochemical behavior of interaction of MnL₂Cl₂ with salmon sperm DNA was investigated on glassy carbon electrode (GCE). In the presence of salmon sperm DNA, the peak current of [MnL₂]²⁺ was decreased and the peak potential was shifted positively without appearance of new peaks. The binding ratio between [MnL₂]²⁺ and salmon sperm DNA was calculated to be 2:1 and the binding constant was 3.72 × 10⁸ mol² L⁻². The extent of hybridization was evaluated on the basis of the difference between signals of [MnL₂]²⁺ with probe DNA before and after hybridization with complementary sequence. Control experiments performed with non-complementary and mismatch sequence demonstrated the good selectivity of the biosensor. With this approach, a sequence of the HBV could be quantified over the range from 1.76 × 10⁻⁸ to 1.07 × 10⁻⁶ mol L⁻¹, with a linear correlation of r = 0.9904 and a detection limit of 6.80 × 10⁻⁹ mol L⁻¹. Additionally, the binding mechanism was preliminarily discussed. The mode of interaction between MnL₂Cl₂ and DNA was found to be primary intercalation binding.     

Strong convergence theorems for a new iterative method of -strictly pseudo-contractive mappings in Hilbert spaces

In this paper, we introduce a new iterative method of a k-strictly pseudo-contractive mapping for some 0≤k<1 and prove that the sequence {x_n} converges strongly to a fixed point of T, which solves a variational inequality related to the linear operator A. Our results have extended and improved the corresponding results of Y.J. Cho, S.M. Kang and X. Qin [Some results on k-strictly pseudo-contractive mappings in Hilbert spaces, Nonlinear Anal. 70 (2008) 1956–1964], and many others.     

The -basis and implicitization of a rational parametric surface

The concept of a μ-basis was introduced in the case of parametrized curves in 1998 and generalized to the case of rational ruled surfaces in 2001. The μ-basis can be used to recover the parametric equation as well as to derive the implicit equation of a rational curve or surface. Furthermore, it can be used for surface reparametrization and computation of singular points. In this paper, we generalize the notion of a μ-basis to an arbitrary rational parametric surface. We show that: (1) the μ-basis of a rational surface always exists, the geometric significance of which is that any rational surface can be expressed as the intersection of three moving planes without extraneous factors; (2) the μ-basis is in fact a basis of the moving plane module of the rational surface; and (3) the μ-basis is a basis of the corresponding moving surface ideal of the rational surface when the base points are local complete intersections. As a by-product, a new algorithm is presented for computing the implicit equation of a rational surface from the μ-basis. Examples provide evidence that the new algorithm is superior than the traditional algorithm based on direct computation of a Gröbner basis. Problems for further research are also discussed.     

Solvability of nonlocal boundary value problems for ordinary differential equation of higher order with a -Laplacian

Nonlocal boundary value problems at resonance for a higher order nonlinear differential equation with a p-Laplacian are considered in this paper. By using a new continuation theorem, some existence results are obtained for such boundary value problems. An explicit example is also given in this paper to illustrate the main results.