共查询到17条相似文献,搜索用时 265 毫秒
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目的:建立胡麻卵磷脂的高效薄层色谱质量分析方法。方法:将卵磷脂供试品及磷脂酰胆碱标准品溶于氯仿-甲醇(3∶2,V/V),以氯仿-甲醇-水(65∶25∶4,V/V)为展开系统,考察不同显色剂、展开系统、点样量、薄层板、检视方式、温度、相对湿度对卵磷脂供试品中不同组分分离的影响,进行高效薄层色谱定性分析;在确定的色谱条件下,将卵磷脂供试品及磷脂酰胆碱标准品进行展开,扫描磷脂酰胆碱峰面积,绘制标准曲线,外标法测定卵磷脂供试品中磷脂酰胆碱的含量。结果:卵磷脂供试品各组分分离情况良好,适于定性分析;卵磷脂供试品中磷脂酰胆碱的含量为63.33%,板间、板内精密度实验相对标准偏差(relative standard deviation,RSD)分别为1.82%和3.73%,稳定性实验RSD为1.60%,加标平均回收率为98.5%。结论:建立的方法快速准确、重复性好,可为卵磷脂质量分析提供新的技术参考。 相似文献
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中国专利:CN1217383A申请日期:1998-09-30申请号:98114363.6公开日期:1999-05-26该酒的主要成分为大豆卵磷脂和白酒,其重量百分比为大豆卵磷脂1.3%~11%,白酒98.7%~89%,白酒的度数为50~60度,原料先经预处理(包括用丙酮处理后的磷脂,去除乙醛、甲醇后的白酒),经混合搅拌卵磷脂溶解提纯、沉淀、调质检测、过滤及成品分装、老熟酯化等工序制成。该技术以白酒作为基础溶剂直接制得大豆卵磷脂酒,取消了磷脂单独用乙醇溶解提纯的普通工艺,因而能降低成本,工艺科学合… 相似文献
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The effect of soybean lecithin addition on the iron‐catalyzed or chlorophyll‐photosensitized oxidation of emulsions consisting of purified canola oil and water (1:1, w/w) was studied based on headspace oxygen consumption using gas chromatography and hydroperoxide production using the ferric thiocyanate method. Addition levels of iron sulfate, chlorophyll, and soybean lecithin were 5, 4, and 350 mg/kg, respectively. Phospholipids (PLs) during oxidation of the emulsions were monitored by high performance liquid chromatography. Addition of soybean lecithin to the emulsions significantly reduced and decelerated iron‐catalyzed oil oxidation by lowering headspace oxygen consumption and hydroperoxide production. However, soybean lecithin had no significant antioxidant effect on chlorophyll‐photosensitized oxidation of the emulsions. PLs in soybean lecithin added to the emulsions were degraded during both oxidation processes, although there was little change in PL composition. Among PLs in soybean lecithin, phosphatidylethanolamine and phosphatidylinositol were degraded the fastest in the iron‐catalyzed and the chlorophyll‐photosensitized oxidation, respectively. The results suggest that addition of soybean lecithin as an emulsifier can also improve the oxidative stability of oil in an emulsion. 相似文献
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大豆卵磷脂的纯化研究 总被引:4,自引:0,他引:4
为了从大豆油脚中提取大豆卵磷脂,通过真空浓缩、丙酮脱油、乙醇提取、吸附脱色、过滤、浓缩等工艺对提取条件做了系统实验,实验结果表明,丙酮的脱油温度和时间,乙醇的提取温度和时间以及吸附剂的选择是影响提取效率的主要因素。用最佳工艺提取得到了纯度为82%的大豆卵磷脂。 相似文献
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为探讨蛋黄卵磷脂对大脑的营养作用,实验采用有机溶剂法从蛋黄中提取卵磷脂粗品,并经柱硅胶纯化,最终获得纯度为95.67%的卵磷脂精品。将获得的卵磷脂精品通过灌胃法给小白鼠灌服30d后,对小鼠进行Morris水迷宫实验、跳台实验。结果显示:受试小鼠Morris水迷宫逃逸时间缩短(P<0.05),跳台实验潜伏期延长(P<0.05),跳台实验错误次数减少(P<0.05),且灌服70d后基本稳定。说明蛋黄卵磷脂可提高小鼠记忆功能,促进大脑发育。 相似文献
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以大豆粉末磷脂为原料,采用无水乙醇萃取和氧化铝柱色谱相结合的技术,研究了高纯度磷脂酰胆碱的制备方法。乙醇萃取后磷脂酰胆碱的纯度为62.00%,得率为31.94%。主要考察了柱层析过程中的固定相、洗脱液浓度、上样量、料液比及洗脱液流速对分离效果的影响。结果表明:当固定相为100~200 目三氧化二铝、洗脱液为90%乙醇、上样量为1 g/30 g、料液比为1∶12(g/mL)、洗脱液流速为3.0 mL/min时,磷脂酰胆碱的纯度可达到94.52%,回收率为83.71%。该研究结果为进一步探讨工业化制备磷脂酰胆碱的研究提供了技术支持和数据支撑。 相似文献