共查询到20条相似文献,搜索用时 15 毫秒
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Alexander Kharin Olga Syshchyk Alain Geloen Sergey Alekseev Andrey Rogov Vladimir Lysenko Victor Timoshenko 《Science and Technology of Advanced Materials》2015,16(4)
Carbon fluoroxide (CFO) nanoparticles (NPs) produced from silicon carbide wafers are used as both fluorescent probes and sonosensitizers for theranostic application. In vitro cell tests were carried out to investigate the feasibility of ultrasound-based therapy with the use of the CFO NPs. The NPs that penetrated inside the cells were shown to provoke cell destruction after application of an ultrasound treatment. No significant toxic effect was observed when the cells were treated with NP concentrations up to 0.5 mg ml−1 without applying ultrasound treatment. The obtained results open a new way toward cancer therapy strategies. 相似文献
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Integrating Oxaliplatin with Highly Luminescent Carbon Dots: An Unprecedented Theranostic Agent for Personalized Medicine 下载免费PDF全文
Min Zheng Shi Liu Jing Li Dan Qu Haifeng Zhao Xingang Guan Xiuli Hu Zhigang Xie Xiabin Jing Zaicheng Sun 《Advanced materials (Deerfield Beach, Fla.)》2014,26(21):3554-3560
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Parthasarathy V Fery-Forgues S Campioli E Recher G Terenziani F Blanchard-Desce M 《Small (Weinheim an der Bergstrasse, Germany)》2011,7(22):3219-3229
Two related triphenylamine-based dipolar and octupolar fluorophores are used to prepare aqueous suspensions of fluorescent organic nanoparticles (FONs) via the reprecipitation method. The obtained spherical nanoparticles (30-40 nm in diameter) are fluorescent in aqueous solution (up to 15% fluorescence quantum yield) and exhibit extremely high one- and two-photon brightness, superior to those obtained for quantum dots. Despite the two chromophores showing similar fluorescence in solution, the fluorescence of FONs made from the octupolar derivative is significantly red-shifted compared to that generated by the dipolar FONs. In addition, the maximum two-photon absorption cross section of the FONs made from the octupolar derivative is 55% larger than that of the dipolar derivative FONs. The experimental observations provide evidence that the different molecular shape (rodlike versus three-branched) and charge distribution (dipolar versus octupolar) of the two chromophores strongly affect the packing inside the nanoparticles as well as their spectroscopic properties and colloidal stability in pure water. The use of these FONs as probes for biphotonic in-vivo imaging is investigated on Xenopus laevis tadpoles to test their utilization for angiography. When using FONs made from the octupolar dye, the formation of microagglomerates (2-5 μm scale) is observed in vivo, with subsequent lethal occlusion of the blood vessels. Conversely, the nanoparticles of the dipolar dye allow acute imaging of blood vessels thanks to their suitable size and brightness, while no toxic effect is observed. Such a goal cannot be achieved with the dissolved dye, which permeates the vessel walls. 相似文献
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Chatterjee DK Gnanasammandhan MK Zhang Y 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(24):2781-2795
Fluorescent labels have been widely used for biological applications, primarily in imaging and assays. Traditional fluorophores such as fluorescent dyes are mainly based on downconversion fluorescence, which have several drawbacks such as photobleaching, high background noise from autofluorescence, and considerable photodamage to biological materials. Upconverting fluorescent nanoparticles emit detectable photons of higher energy in the near-infrared (NIR) or visible range upon irradiation with an NIR light in a process termed 'upconversion.' They overcome some of the disadvantages faced by conventional downconversion labels, thus making them an ideal fluorescent label for biological applications. This review looks at the development of these particles, critically examines the reported applications, and discusses their future in biomedicine. 相似文献
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Glowing Graphene Quantum Dots and Carbon Dots: Properties,Syntheses, and Biological Applications 下载免费PDF全文
Xin Ting Zheng Arundithi Ananthanarayanan Kathy Qian Luo Peng Chen 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(14):1620-1636
The emerging graphene quantum dots (GQDs) and carbon dots (C‐dots) have gained tremendous attention for their enormous potentials for biomedical applications, owing to their unique and tunable photoluminescence properties, exceptional physicochemical properties, high photostability, biocompatibility, and small size. This article aims to update the latest results in this rapidly evolving field and to provide critical insights to inspire more exciting developments. We comparatively review the properties and synthesis methods of these carbon nanodots and place emphasis on their biological (both fundamental and theranostic) applications. 相似文献
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Małgorzata Misiak Michał Skowicki Tomasz Lipiński Agnieszka Kowalczyk Katarzyna Prorok Sebastian Arabasz Artur Bednarkiewicz 《Nano Research》2017,10(10):3333-3345
Versatile optimization of the synthesis method and composition of Yb3+ and Tm3+ co-doped CaF2 nanoparticles as well as a novel biofunctionalization method were developed and evaluated.Through multistep synthesis,the luminescence intensity of the Tm3+ activator was enhanced by more than 10-fold compared to standard one-step synthesis.The proposed methods were used to homogenously distribute the doping ions within the nanoparticle's volume and thus reduce luminescence quenching.Optimization of dopant ions concentration led to the selection of the most efficient visible and near-infrared up-converting nanoparticles,which were CaF2 doped with 10% Yb3+ 0.05% Tm3+ and 20% Yb3+ 0.5% Tm3+,respectively.To illustrate the suitability of the synthesized nanoparticles as bio-labels,a dedicated biofunctionalization method was used,and the nanoparticles were applied for labeling and imaging of Candida albicans cells.This method shows great promise because of extremely low background and high specificity because of the presence of the attached molecules. 相似文献
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3C-SiC nanocrystals as fluorescent biological labels 总被引:1,自引:0,他引:1
Fan J Li H Jiang J So LK Lam YW Chu PK 《Small (Weinheim an der Bergstrasse, Germany)》2008,4(8):1058-1062
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碳量子点的合成、性质及其应用 总被引:2,自引:0,他引:2
碳量子点(CQDs,C-dots or CDs)是一种新型的碳纳米材料,尺寸在10nm以下,具有良好的水溶性、化学惰性、低毒性、易于功能化和抗光漂白性、光稳定性等优异性能,是碳纳米家族中的一颗闪亮的明星。自从2006年[1]报道了碳量子点(CQDs)明亮多彩的发光现象后,世界各地的研究小组开始对CQDs进行了深入的研究。最近几年的研究报道了各种方法制备的CQDs在生物医学、光催化、光电子、传感等领域中都有重要的应用价值。这篇综述主要总结了关于CQDs的最近的发展,介绍了CQDs的合成方法、表面修饰、掺杂、发光机理、光电性质以及在生物医学、光催化、光电子、传感等领域的应用。 相似文献
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Tunable Luminescent Carbon Nanospheres with Well‐Defined Nanoscale Chemistry for Synchronized Imaging and Therapy 下载免费PDF全文
Prabuddha Mukherjee Santosh K. Misra Mark C. Gryka Huei‐Huei Chang Saumya Tiwari William L. Wilson John W. Scott Rohit Bhargava Dipanjan Pan 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(36):4691-4703
In this work, we demonstrate the significance of defined surface chemistry in synthesizing luminescent carbon nanomaterials (LCN) with the capability to perform dual functions (i.e., diagnostic imaging and therapy). The surface chemistry of LCN has been tailored to achieve two different varieties: one that has a thermoresponsive polymer and aids in the controlled delivery of drugs, and the other that has fluorescence emission both in the visible and near‐infrared (NIR) region and can be explored for advanced diagnostic modes. Although these particles are synthesized using simple, yet scalable hydrothermal methods, they exhibit remarkable stability, photoluminescence and biocompatibility. The photoluminescence properties of these materials are tunable through careful choice of surface‐passivating agents and can be exploited for both visible and NIR imaging. Here the synthetic strategy demonstrates the possibility to incorporate a potent antimetastatic agent for inhibiting melanomas in vitro. Since both particles are Raman active, their dispersion on skin surface is reported with Raman imaging and utilizing photoluminescence, their depth penetration is analysed using fluorescence 3D imaging. Our results indicate a new generation of tunable carbon‐based probes for diagnosis, therapy or both. 相似文献
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Jianjun Du Ning Xu Jiangli Fan Wen Sun Xiaojun Peng 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(32)
Carbon dots (CDs), a kind of carbon material discovered accidentally, exhibit unexpected advantages in fluorescence imaging/sensing such as photostability, biocompatibility, and low toxicity. For emerging theranostics, an interdiscipline created by integrating therapy and diagnostics, CDs are good candidates when they are combined with targeted chemo/gene/photodynamic/photothermal therapeutic moieties. Here, the development of CDs in nanomedicine is reviewed from their use as original imaging agents and/or drug carriers to multifunctional theranostic systems. Finally, the challenges and prospects of the next‐generation of CD‐based theranostics for clinical applications are also discussed. 相似文献
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Luminescent carbon nanoparticles (CNPs) are newcomers to the world of nanomaterials and have shown great impact in health
and environmental applications as well as being promising building blocks for future nanodevices because of their fascinating
photoluminescence and potential to serve as nontoxic replacements for traditional heavy-metals-based quantum dots. Herein,
fluorescent CNPs have been prepared from candle soot by refluxing with HNO3 and subsequently separated by a single centrifugation. The CNPs can be represented by the empirical formula C1H0.677O0.586N0.015Na0.069, and have a size of 20–100 nm, height of 3.0 nm, lifetime of 7.31 ns ± 0.06 ns and quantum yield of ∼1.7%. Further studies
demonstrate that: (1) the as-prepared CNPs exhibit excellent stability in biological media and their luminescence intensity
does not change with ionic strength or pH in the physiological and pathological range of pH 4.5–8.8; (2) CNPs can act as electron
donors and transporters and porphyrin can assemble onto CNPs through electrostatic and π-stacking interactions to form porphyrin-CNPs
supramolecular composites; (3) CNPs have strong intrinsic peroxidase-like activity. Based on this intrinsic peroxidase activity,
a simple, cheap, and highly selective and sensitive colorimetric and quantitative assay has been developed for the detection
of glucose levels. This assay has been used to analyze real samples, such as diluted blood and fruit juice.
相似文献
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Al-Jamal WT Al-Jamal KT Bomans PH Frederik PM Kostarelos K 《Small (Weinheim an der Bergstrasse, Germany)》2008,4(9):1406-1415
Functionalized-quantum-dot-liposome (f-QD-L) hybrid nanoparticles are engineered by encapsulating poly(ethylene glycol)-coated QD in the internal aqueous phase of different lipid bilayer vesicles. f-QD-L maintain the QD fluorescence characteristics as confirmed by fluorescence spectroscopy, agarose gel electrophoresis, and confocal laser scanning microscopy. Cationic f-QD-L hybrids lead to dramatic improvements in cellular binding and internalization in tumor-cell monolayer cultures. Deeper penetration into three-dimensional multicellular spheroids is obtained for f-QD-L by modifying the lipid bilayer characteristics of the hybrid system. f-QD-L are injected intratumorally into solid tumor models leading to extensive fluorescent staining of tumor cells compared to injections of the f-QD alone. f-QD-L hybrid nanoparticles constitute a versatile tool for very efficient labeling of cells ex vivo and in vivo, particularly when long-term imaging and tracking of cells is sought. Moreover, f-QD-L offer many opportunities for the development of combinatory therapeutic and imaging (theranostic) modalities by incorporating both drug molecules and QD within the different compartments of a single vesicle. 相似文献
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Ahmed A. H. Abdellatif 《Drug development and industrial pharmacy》2018,44(10):1679-1684
Objective: It is difficult to identify the gold nanoparticles (AuNPs) intracellularly due to their non-fluorescent nature. Although gold can quench the fluorescence of any fluorophore, hence it is also difficult to combine gold with a fluorophore such as a semiconductor quantum dots (QDs). The aim of this study was to prepare a single fluorescent stable AuNPs combined with QDs (QDs-Au-NPs) which can be easily detected intracellularly.Methods: QDs-Au-NPs were prepared via a simple one-step process through controlling the spacing between them using polyethylene glycol (PEG) as space linker in the form of PEGylated QDs. Furthermore, the applicability of this system was evaluated after coating the particles with somatostatin citrate, SST, to active target somatostatin receptors (SSTRs), and identification of the internalized particles via confocal laser scanning spectroscopy.Results: The results showed that the produced Au shell has a thickness of 2.0?±?0.2?nm and QDs-Au-NPs showed the same fluorescence intensity compared to the unmodified QDs. Additionally, a stable monodisperse QDs-Au-NPs coated with SST were prepared after coating with 11-Mercaptoundecanoic acid. Moreover, cellular uptake study in Human Caucasian breast adenocarcinoma cell lines showed that QDs-Au-SST-NPs could be detected easily using the confocal microscope. In addition, they showed a significant (p?≤?.05) internalization per cell compared to untreated QDs-Au-NPs as detected by flow cytometry.Conclusion: It could be concluded that the produced QDs-Au-NPs has a strong fluorescence property like QDs which enable them to be easily detected after cells internalization. 相似文献
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Carbon nanotubes are one of the most intensively explored nanostructured materials. In particular, carbon nanotubes are unique and ideal templates onto which to immobilize nanoparticles allowing the construction of designed nanoarchitectures that are extremely attractive as supports for heterogeneous catalysts, for use in fuel cells, and in related technologies that exploit the inherent 'smallness' and hollow characteristics of the nanoparticles. Here we overview the recent developments in this area by exploring the various techniques in which nanotubes can be functionalized with metals and other nanoparticles and explore the diverse applications of the resulting materials. 相似文献
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采用直接沉淀法,通过改变沉淀剂(氨水、NaOH)及反应时间制备纳米ZnO粒子,并通过扫描电子显微镜、荧光显微镜对所得产物的形貌及其荧光性能进行分析表征。结果显示:不同沉淀剂制备的ZnO纳米粒子的形貌和尺寸均不相同,用Zn(NO3)2和NH3.H2O反应可制得花状的纳米粒子,而用Zn(NO3)2和NaOH反应则会生成球状的纳米粒子,其尺寸分别为500和200nm左右;反应前期,反应时间主要影响粒子的尺寸,随反应时间的增加粒子的形貌也发生变化;与以NaOH为沉淀剂制备的纳米ZnO粒子相比,以氨水为沉淀剂制备的纳米ZnO粒子具有好的荧光性能。 相似文献