共查询到20条相似文献,搜索用时 15 毫秒
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《Food chemistry》2005,93(1):47-56
The optimum conditions for the extraction of crude phenolics from whole grain and bran of soft and hard wheat were determined using response surface methodology (RSM). A face-centered cubic design (FCD) was used to investigate the effects of three independent variables, namely solvent composition (%), extraction temperature (°C) and time (min) on the response, total antioxidant activity (TAA). The independent variables were coded at three levels and their actual values selected on the basis of preliminary experimental results. The FCD consisted of 14 experimental points and three replications at the center point. Data were analyzed using design expert and statistical analysis system software. A second-order polynomial model was used for predicting the response. Regression analysis showed that more than 89% of the variation was explained by the models. Canonical analysis of surface responses revealed that the stationary surface was a saddle. The optimal conditions forthe TAA obtained using ridge analysis were 54%, 61 °C, 64 min and 49%, 64 °C, 60 min, for whole grain and bran of soft wheat, respectively. Under the optimum conditions the corresponding predicted response values for TAA were 56.5 and 63 TE. The crude phenolics were extracted under optimum conditions to check the validity of the model. The values were 54.7 ± 3.2 and 61.3 ± 1.9 TE, for whole grain and bran of soft wheat, respectively; A similar trend was observed for TAA of hard wheat. The experimental values agreed with those predicted, thus indicating suitability of the model employed and the success of RSM in optimizing the extraction conditions. 相似文献
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目的:对天麻总多酚的提取工艺进行优化。方法:以天麻为材料在单因素实验结果的基础上,采用三因素三水平的Box-Behnken响应曲面设计。结果:天麻提取的最佳条件为乙醇浓度57%,提取温度69℃,提取时间53min,该条件下多酚提取含量达44.96mg GAE/g,且提取温度对总多酚的得率影响最大。结论:在最佳条件下天麻多酚得率实际值是预测值的98.48%,接近预测值,可用于天麻多酚的提取。 相似文献
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采用超声波辅助提取猕猴桃果皮多酚,并利用响应面法对多酚提取工艺进行优化。在单因素实验的基础上,采用四因素三水平的响应面实验优化设计,研究超声波功率、提取时间、提取温度、液料比对多酚提取量的影响。结果显示最佳提取工艺条件为:超声波功率384.00 W,提取时间30 min,提取温度65.00℃,液料比23.00 m L/g,多酚提取量的实验值为(28.10±0.38)mg GAE/g,与理论预测值(28.14 mg GAE/g)相差不大。通过体外1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除力测定多酚的抗氧化性,并且得到猕猴桃果皮多酚的EC50值为0.13 mg/m L,说明提取的多酚具有很好的抗氧化性。 相似文献
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Caroline Stévigny Luca Rolle Nadia Valentini Giuseppe Zeppa 《Journal of the science of food and agriculture》2007,87(15):2817-2822
Response surface methodology was applied to predict the optimum conditions for extraction of phenolic compounds in hazelnut shell. The phenolic content in the shell extract was determined spectrophotometrically according to the Folin‐Ciocalteu method and expressed as gallic acid equivalent (mg GAE g?1). Two central composite designs were used to investigate the effects of two independent variables, namely solvent composition (%) and extraction time (min), on phenolic extraction. In a first series of repeated batch extractions, the solvent consisted of different methanol percentages in distilled water at pH 4, while in a second series methanol was substituted by ethanol. The highest phenolic content (6.67 mg GAE g?1 of shell) was predicted at the extraction conditions of 55.7% ethanol and 108.7 min. These best conditions, obtained and applied to 13 different cultivars, showed values varying from 9.18 mg GAE g?1 of shell for Barcelona to 3.00 mg GAE g?1 of shell for Tonda di Giffoni. Copyright © 2007 Society of Chemical Industry 相似文献
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Optimization of the extraction of total polyphenols (TPC), flavanols (FL), total anthocyanins (TA) and total tannins (TT) from grape marc by the response surface methodology is important to improve the recovery of these high-value phytochemicals. The independent variables studied were ethanol concentration (% EtOH), extraction time (h) and liquid-to-solvent ratio (L/S). TPC, FL, TT and TA showed different patterns of extractability, with significant variation in the linear, quadratic and interaction effects of the independent variables. The optimal extraction conditions were determined and the quadratic response surfaces were drawn from the mathematical models. The optimal extraction conditions were: 57% EtOH, 17?h, 50:1 L/S for TPC; 57% EtOH, 13?h, 50:1 L/S for FL; 62% EtOH, 16?h, 50:1 L/S for TT and 52% EtOH, 6?h, 10:1 L/S for TA. These optimum conditions yielded: 24?mg GAE/g DM of TPC; 1.5?mg quercetin/g DM of FL; 18?mg catechin/g DM of TT and 6?mg malvidin/g DM of TA. 相似文献
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Hyo Seon Kim A. Yeong Lee Ji Eun Jo Byeong Cheol Moon Jin Mi Chun Goya Choi Ho Kyoung Kim 《Food science and biotechnology》2014,23(1):1-7
In this study, the optimal conditions for quercitrin extraction from Houttuynia cordata Thunb. (HC) were evaluated using the response surface methodology (RSM). The quercitrin was analyzed and method validated by ultra performance liquid chromatography carried out with a photodiode array detector (UPLC-PDA). Central composite design (CCD), a type of RSM, was applied to estimate the influence of three independent variables, the extraction temperature, extraction time, and extraction concentration, on the quercitrin yield. Analysis of variance (ANOVA) showed a good model fit (r 2=0.8863). The predicted optimal quercitrin yield was 1.497% under an extraction temperature of 80°C and an extraction time of 34 min in a solvent of 95% methanol. Using these optimal conditions, the experimentally obtained quercitrin yield was 1.483% (±0.03), which agrees closely with the predicted value. 相似文献
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新疆红肉苹果多酚的超声波辅助提取工艺优化 总被引:1,自引:0,他引:1
利用响应面法优化超声波辅助提取新疆红肉苹果多酚的工艺条件。以新疆红肉苹果为试验原料,在单因素试验基础上选择提取时间、超声功率、提取温度及料液比4个因素进行响应面设计,采用Box-Behnken中心组合试验设计和响应面分析法,确定红肉苹果多酚的最佳提取工艺。结果表明,红肉苹果多酚的最佳提取参数为:料液比14(g/mL),超声功率360 W,提取温度62℃,提取时间20min。在该最佳条件下,新疆红肉苹果多酚得率达到2.135mg/g。该结果可为苹果多酚的实际生产提供借鉴。 相似文献
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为了研究超声波辅助提取蚕豆多酚的最佳工艺条件,在单因素实验的基础上,选取乙醇体积分数、料液比、提取时间、pH为自变量,多酚提取量为响应值,根据Box-Benhnken实验设计原理,采用四因素三水平的分析法对蚕豆多酚提取量进行优化。结果表明,超声波辅助提取蚕豆多酚的最佳工艺条件为:乙醇体积分数45%、料液比1:25 (g/mL)、提取时间20 min、pH5,此条件下蚕豆多酚提取量最高为(2.03±0.16) mg/g d.w.,与预测值(2.06±0.23) mg/g d.w.相近,说明该优化方法合理可行。 相似文献