共查询到20条相似文献,搜索用时 0 毫秒
1.
Lei Zhao Guang-Li Cao Ai-Jie Wang Hong-Yu Ren Nan-Qi Ren 《International Journal of Hydrogen Energy》2013
Enzymatically treated cornstalk hydrolysate was tested as substrate for H2 production by Thermoanaerobacterium thermosaccharolyticum W16 in a continuous stirred tank reactor. The performance of strain W16 to ferment the main components of hydrolysate, mixture of glucose and xylose, in continuous culture was conducted at first, and then T. thermosaccharolyticum W16 was evaluated to ferment fully enzymatically hydrolysed cornstalk to produce H2 in continuous operation mode. At the dilution rate of 0.020 h−1, the H2 yield and production rate reached a maximum of 1.9 mol H2 mol−1 sugars and 8.4 mmol H2 L−1 h−1, respectively, accompanied with the maximum glucose and xylose utilizations of 86.3% and 77.6%. Continuous H2 production from enzymatically treated cornstalk hydrolysate in this research provides a new direction for economic, efficient, and harmless H2 production. 相似文献
2.
Guo-Jun Xie Bing-Feng LiuJie Ding De-Feng XingJun Nan Hong-Yu RenNan-Qi Ren 《International Journal of Hydrogen Energy》2012
A novel photo-fermentative sequencing batch reactor (PFSBR) process assisted by activated carbon fibers (ACFs) was used to continuously produce hydrogen gas by Rhodopseudomonas faecalis RLD-53. Feasibility of continuous hydrogen production in PFSBR operation at different hydraulic retention times (HRTs) (48, 96, 144 and 192 h) and influent acetate concentrations (20, 40, 60 and 80 mmol/l) was investigated. The rate and yield of hydrogen production increased with HRTs from 48 to 144 h, and then decreased with the HRTs from 144 to 192 h. Regulation of the proper influent acetate concentration (60 mmol/l) not only increased hydrogen production by PFSBR, but also maintained quality of the effluent with high substrate removal efficiency (97.70%). Free R. faecalis RLD-53 was adsorbed on the surface of ACFs, initially isolated cells, then monolayer, and finally mature biofilm with three dimensional multilayers structures. The PFSBR reached a maximum hydrogen yield (3.12 mol H2/mol acetate), and achieved a steady state when mature biofilm developed on ACFs. Therefore, photo-fermentative sequencing batch reactor was a promising process for continuous photo-fermentative hydrogen production. 相似文献
3.
《International Journal of Hydrogen Energy》2022,47(9):5879-5890
Bio-hydrogen production in a continuously operated anaerobic packed bed biofilm reactor (APBR) using acid-hydrolysate of rice straw as feedstock and inoculated with an anaerobic mesophilic sludge from a municipal wastewater treatment plant was investigated at three different HRTs (17, 8.2 and 2 h). Fermentable sugars solution achieved from a two-stage diluted acid hydrolysis of rice straw was used as the feedstock. First, rice straw was treated with 1% w v?1 sulfuric acid at 120 °C for 30 min with a yield of 58.5% xylose. Higher temperature of 180 °C for 10 min at 0.5% w v?1 sulfuric acid was applied in the second stage in which cellulosic crystalline structure was partially depolymerized to glucose with a yield of 19.3% glucose. Hydrogen production rate and yield were enhanced as the hydraulic retention time was decreased with a maximum production rate of 252 mL L?1 h?1 and yield of 1 mol H2 mol?1 sugar consumed at 2 h HRT. Experimental results illustrated the increase of COD conversion from 44% to 47% by shortening the HRT from 17 to 2 h. Furthermore, acetic acid and butyric acid production were reduced slower than other soluble metabolites like ethanol. 相似文献
4.
Biological hydrogen production in an anaerobic sequencing batch reactor: pH and cyclic duration effects 总被引:1,自引:0,他引:1
An anaerobic sequencing batch reactor (ASBR) was used to evaluate biological hydrogen production from carbohydrate-rich organic wastes. The goal of the proposed project was to investigate the effects of pH (4.9, 5.5, 6.1, and 6.7), and cyclic duration (4, 6, and 8 h) on hydrogen production. With the ASBR operated at 16-h HRT, 25 g COD/L, and 4-h cyclic duration, the results showed that the maximum hydrogen yield of 2.53 mol H2/mol sucroseconsumed appeared at pH 4.9. The carbohydrate removal efficiency declined to 56% at pH 4.9, which indirectly resulted in the reduction of total volatile fatty acid production. Acetate fermentation was the dominant metabolic pathway at pH 4.9. The concentration of mixed liquor volatile suspended solid (MLVSS) also showed a decrease from nearly 15,000 mg/L between pHs 6.1 and 6.7 to 6000 mg/L at pH 4.9. Investigation of the effect of cyclic duration found that hydrogen yield reached the maximum of 1.86 mol H2/mol sucroseconsumed at 4-h cyclic duration while ASBR was operating at 16-h HRT, 15 g COD/L, and pH 4.9. The experimental results showed that MLVSS concentration increased from 6200 mg/L at 4-h cyclic duration to 8500 mg/L at 8-h cyclic duration. However, there was no significant change in effluent volatile suspended solid concentration. The results of butyrate to acetate ratio showed that using this ratio to correlate the performance of hydrogen production is not appropriate due to the growth of homoacetogens. In ASBR, the operation is subject to four different phases of each cycle, and only the complete mix condition can be achieved at react phase. The pH and cyclic duration under the unique operations profoundly impact fermentative hydrogen production. 相似文献
5.
Pawinee SearmsirimongkolPramoch Rangsunvigit Malinee LeethochawalitSumaeth Chavadej 《International Journal of Hydrogen Energy》2011,36(20):12810-12821
In this study, the feasibility of hydrogen production from alcohol distillery wastewater containing high potassium and sulfate was investigated using an anaerobic sequencing batch reactor (ASBR). The seed sludge taken from an anaerobic tank treating the distillery wastewater was boiled for 15 min before being fed to the ASBR. The ASBR system was operated under different feed chemical oxygen demand (COD) values and different COD loading rates at a mesophilic temperature of 37 °C, a controlled pH at 5.5, and a cycle time of 6 cycles per day. When the studied ASBR was operated under the best conditions (providing a maximum hydrogen production efficiency) of a feed COD of 40,000 mg/l, a COD loading rate of 60 kg/m3 d, and a hydraulic retention time of 16 h, the produced gas was found to contain 34.7% H2 and 65.3% CO2, without any methane being detected. Under these best conditions, the specific hydrogen production rate (SHPR) of 270 ml H2/g MLVSS d (or 3310 ml H2/l d), and hydrogen yield of 172 ml H2/g COD removed, were obtained. When the feed COD exceeded 40,000 mg/l, the process performance in terms of hydrogen production decreased because of the potassium and sulfate toxicity. 相似文献
6.
Piyawadee Saraphirom Alissara Reungsang 《International Journal of Hydrogen Energy》2011,36(14):8765-8773
Continuous biological hydrogen production from sweet sorghum syrup by mixed cultures was investigated by using anaerobic sequencing batch reactor (ASBR). The ASBR was conducted based on the optimum condition obtained from batch experiment i.e. 25 g/L of total sugar concentration, 1.45 g/L of FeSO4 and pH of 5.0. Feasibility of continuous hydrogen fermentation in ASBR operation at room temperature (30 ± 3 °C) with different hydraulic retention time (HRT) of 96, 48, 24 and 12 hr and cycle periods consisting of filling (20 min), settling (20 min), and decanting (20 min) phases was analyzed. Results showed that hydrogen content decreased with a reduction in HRT i.e. from 42.93% (96 hr HRT) to 21.06% (12 hr HRT). Decrease in HRT resulted in a decrease of solvents produced which was from 10.77 to 2.67 mg/L for acetone and 78.25 mg/L to zero for butanol at HRT of 96 hr-12 hr, respectively. HRT of 24 hr was the optimum condition for ASBR operation indicated by the maximum hydrogen yield of 0.68 mol H2/mol hexose. The microbial determination in DGGE analysis indicated that the well-known hydrogen producers Clostridia species were dominant in the reacting step. The presence of Sporolactobacillus sp. which could excrete the bacteriocins causing the adverse effect on hydrogen-producing bacteria might responsible for the low hydrogen content obtained. 相似文献
7.
Marzieh BadieiJamaliah Md Jahim Nurina AnuarSiti Rozaimah Sheikh Abdullah 《International Journal of Hydrogen Energy》2011,36(10):5912-5919
The feasibility of hydrogen generation from palm oil mill effluent (POME), a high strength wastewater with high solid content, was evaluated in an anaerobic sequencing batch reactor (ASBR) using enriched mixed microflora, under mesophilic digestion process at 37 °C. Four different hydraulic retention times (HRT), ranging from 96 h to 36 h at constant cycle length of 24 h and various organic loading rate (OLR) concentrations were tested to evaluate hydrogen productivity and operational stability of ASBR. The results showed higher system efficiency was achieved at HRT of 72 h with maximum hydrogen production rate of 6.7 LH2/L/d and hydrogen yield of 0.34 LH2/g CODfeeding, while in longer and shorter HRTs, hydrogen productivity decreased. Organic matter removal efficiency was affected by HRT; accordingly, total and soluble COD removal reached more than 37% and 50%, respectively. Solid retention time (SRT) of 4-19 days was achieved at these wide ranges of HRTs. Butyrate was found to be the dominant metabolite in all HRTs. Low concentration of volatile fatty acid (VFA) confirmed the state of stability and efficiency of sequential batch mode operation was achieved in ASBR. Results also suggest that ASBR has the potential to offer high digestion rate and good stability of operation for POME treatment. 相似文献
8.
Phatlapha Thanwised Wanpen Wirojanagud Alissara Reungsang 《International Journal of Hydrogen Energy》2012
This study evaluated hydrogen production and chemical oxygen demand removal (COD removal) from tapioca wastewater using anaerobic mixed cultures in anaerobic baffled reactor (ABR). The ABR was conducted based on the optimum condition obtained from the batch experiment, i.e. 2.25 g/L of FeSO4 and initial pH of 9.0. The effects of the varying hydraulic retention times (HRT: 24, 18, 12, 6 and 3 h) on hydrogen production and COD removal in a continuous ABR were operated at room temperature (32.3 ± 1.5 °C). Hydrogen production rate (HPR) increased with a reduction in HRT i.e. from 164.45 ± 4.14 mL H2/L.d (24 h HRT) to 883.19 ± 7.89 mL H2/L.d (6 h HRT) then decreased to 748.54 ± 13.84 mL H2/L.d (3 h HRT). COD removal increased with reduction in HRT i.e. from 14.02 ± 0.58% (24 h HRT) to 29.30 ± 0.84% (6 h HRT) then decreased to 21.97 ± 0.94% (3 h HRT). HRT of 6 h was the optimum condition for ABR operation as indicated. 相似文献
9.
pH is considered as one of the most important factors governing the hydrogen fermentation process. In this project, five pH levels, ranging from 4.4 to 5.6 at 0.3 increments, were tested to evaluate the pH effect on hydrogen production from swine manure supplemented with glucose in an anaerobic sequencing batch reactor system with 16 h of hydraulic retention time (HRT). The optimal hydrogen yield (1.50 mol H2/mol glucose) was achieved at pH 5.0 when the maximum production rate of 2.25 L/d/L was obtained. Continuous hydrogen production was achieved for over 3 weeks for pH 5.0, 4.7, and 4.4, with no significant methane produced. However, as pH increased to 5.3 and 5.6, methane production was observed in the biogas with concurrent reductions in hydrogen production, indicating that methanogens could become increasingly activated for pH 5.3 or higher. Acetate, propionate, butyrate, valerate, and ethanol were the main aqueous products whose distribution was significantly affected by pH as well. 相似文献
10.
Sonia ArriagaIzcoatl Rosas Felipe Alatriste-MondragónElías Razo-Flores 《International Journal of Hydrogen Energy》2011,36(5):3442-3449
Hydrogen was produced in a biotrickling filter (BF) packed with perlite and fed with oat straw acid hydrolysate at 30 °C. Inlet chemical oxygen demand (COD) from 1.2 to 35 g/L and hydraulic retention time (HRT) between 24 h and 6 h were assayed. With increasing inlet COD or decreasing HRT, H2 production rate (HPR) increased but H2 production yield (HY) decreased. Maximum HPR of 81.4 mL H2/Lreactor h (3.3 mmol H2/Lreactor h) and HY of 2.9 mol H2/molhexose consumed were found at an inlet COD of 0.05 gCOD/L h (HRT 24 h) and 2.9 gCOD/L h (HRT 12 h), respectively. Maximum hydrogen composition in gas was 45 ± 4% (v/v) with CO2 as balance. Methane was not detected. Maximum HPR and inlet COD used in this work were higher than others reported for reactors with suspended or fixed biomass. However, implementation of strategies for biomass control to avoid reactor clogging is needed. 相似文献
11.
Xiao Wu Jun ZhuChunying Dong Curt MillerYecong Li Liang WangWanying Yao 《International Journal of Hydrogen Energy》2009
Liquid swine manure supplemented with glucose (10 g/L) was used as substrate for hydrogen production using an anaerobic sequencing batch reactor at 37 ± 1 °C and pH 5.0 under different hydraulic retention times (HRTs). Decreasing HRT from 24 to 8 h caused an increasing hydrogen production rate from 0.05 to 0.15 L/h/L. Production rates of both total biogas and hydrogen were linearly correlated to HRT with R2 being 0.993 and 0.997, respectively. The hydrogen yield ranged between 1.18 and 1.63 mol-H2/mol glucose and the 12 h HRT was preferred for high production rate and efficient yield. For all the five HRTs examined, the glucose utilization efficiency was over 98%. The biogas mainly consisted of carbon dioxide and hydrogen (up to 43%) with no methane detected throughout the experiment. Ethanol and organic acids were the major aqueous metabolites produced during fermentation, with acetic acid accounting for 56–58%. The hydrogen yield was found to be related to the acetate/butyrate ratio. 相似文献
12.
Nan-Qi Ren Guang-Li CaoWan-Qian Guo Ai-Jie WangYu-Hong Zhu Bing-feng LiuJi-Fei Xu 《International Journal of Hydrogen Energy》2010
This study addressed the utilization of an agro-waste, corn stover, as a renewable lignocellulosic feedstock for the fermentative H2 production by the moderate thermophile Thermoanaerobacterium thermosaccharolyticum W16. The corn stover was first hydrolyzed by cellulase with supplementation of xylanase after delignification with 2% NaOH. It produced reducing sugar at a yield of 11.2 g L−1 glucose, 3.4 g L−1 xylose and 0.5 g L−1 arabinose under the optimum condition of cellulase dosage 25 U g−1 substrate with supplement xylanase 30 U g−1 substrate. The hydrolyzed corn stover was sequentially introduced to fermentation by strain W16, where, the cell density and the maximum H2 production rate was comparable to that on simulated medium, which has the same concentration of reducing sugars with hydrolysate. The present results suggest a promising combined hydrogen production process from corn stover with enzymatic hydrolysis stage and fermentation stage using W16. 相似文献
13.
Acid hydrolysis of corn stover for biohydrogen production using Thermoanaerobacterium thermosaccharolyticum W16 总被引:1,自引:0,他引:1
Guangli Cao Nanqi Ren Aijie Wang Duu-Jong Lee Wanqian Guo Bingfeng Liu Yujie Feng Qingliang Zhao 《International Journal of Hydrogen Energy》2009,34(17):7182-7188
Lignocellulosic biomass, if properly hydrolyzed, can be an ideal feedstock for fermentative hydrogen production. This work considered the pretreatment of corn stover (CS) using a dilute acid hydrolysis process and studied its fermentability for hydrogen production by the strain Thermoanaerobacterium thermosaccharolyticum W16. The effects of sulfuric acid concentration and reaction time in the hydrolysis stage of the process were determined based on a 22 central composite experimental design with respect to maximum hydrogen productivity. The optimal hydrolysis conditions to yield the maximum quantity of hydrogen by W16 were 1.69% sulfuric acid and 117 min reaction time. At these conditions, the hydrogen yield was shown to be 3305 ml H2 L−1 medium, which corresponds to 2.24 mol H2 mol−1 sugar. The present results indicate the potential of using T. thermosaccharolyticum W16 for high-yield conversion of CS hemicellulose into bio-H2 integrated with acid hydrolysis. 相似文献
14.
Patcharee Intanoo Thitiporn Suttikul Malinee Leethochawalit Erdogen Gulari Sumaeth Chavadej 《International Journal of Hydrogen Energy》2014
The objective of this study was to investigate the enhancement of hydrogen production from alcohol wastewater by adding fermentation residue using an anaerobic sequencing batch reactor (ASBR) under thermophillic operation (55 °C) and at a constant pH of 5.5. The digestibility of the added fermentation residue was also evaluated. For a first set of previous experiments, the ASBR system was operated to obtain an optimum COD loading rate of 50.6 kg/m3 d of alcohol wastewater without added fermentation residue and the produced gas contained 31% H2 and 69% CO2. In this experiment, the effect of added fermentation residue (100–1200 mg/l) on hydrogen production performance was investigated under a COD loading rate of 50.6 kg/m3 d of the alcohol wastewater. At a fermentation residue concentration of 1000 mg/l, the produced gas contained 40% H2 and 60% CO2 without methane and the system gave the highest hydrogen yield and specific hydrogen production rate of 128 ml/g COD removed and 2880 ml/l d, respectively. Under thermophilic operation with a high total COD loading rate (51.8 kg/m3 d) and a short HRT (21 h) at pH 5.5, the ASBR system could only break down cellulose (41.6%) and hemicellulose (21.8%), not decompose lignin. 相似文献
15.
Shing-Der Chen Yung-Chung Lo Kuo-Shing Lee Tian-I. Huang Jo-Shu Chang 《International Journal of Hydrogen Energy》2009
Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90% discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate. 相似文献
16.
The key factors influencing a co-digestion of the oil palm trunk (OPT) hydrolysate with a slaughterhouse wastewater (SHW) to produce hydrogen by Thermoanaerobacterium thermosaccharolyticum KKU19 were investigated. The OPT hydrolysate was obtained by the hydrolysis of OPT by microwave-H2SO4 method using 1.56% (w/v) H2SO4 and 7.50 min reaction time at 450 W. The Plackett–Burman method was used to screen the key factors that influenced the hydrogen production potential (Ps). Results indicated that initial cell concentration, tCOD/TN (total COD/total nitrogen) ratio and CuSO4 concentration influenced the Ps. These factors were further optimized using response surface methodology (RSM) with central composite design (CCD). A maximum Ps of 2604 ± 86 mL H2/L substrate was achieved at an initial cell concentration of 224 mg dry cell/L, tCOD/TN ratio of 49.87 and CuSO4 concentration of 13.33 mg/L. The main soluble metabolite products were butyric and acetic acids. The Ps obtained when the hydrolysate was supplemented with SHW (2604mL ± 86 mL H2/L substrate) was comparable to the Ps obtained when it was supplemented with yeast extract at the same tCOD/TN (2802 ± 87 mL H2/L substrate). This result suggests that SHW can be used to replace the costly nitrogen source. 相似文献
17.
Patcharee Intanoo Pramoch Rangsunvigit Weerachart Namprohm Bandhit Thamprajamchit Jittipan Chavadej Sumaeth Chavadej 《International Journal of Hydrogen Energy》2012
The objective of this study was to investigate hydrogen production from alcohol wastewater using an anaerobic sequencing batch reactor (ASBR) under thermophilic operation and at a constant pH of 5.5. Under the optimum COD loading rate of 68 kg/m3d, the produced gas contained 43% H2 without methane and the system provided a hydrogen yield and specific hydrogen production rate of 130 ml H2/g COD removed and 2100 ml H2/l d, respectively, which were much higher than those obtained under the mesophilic operation. Under thermophilic operation, both nitrogen and phosphate uptakes were minimal at the optimum COD loading rate for hydrogen production and most nitrogen uptake was derived from organic nitrogen. Under the thermophilic operation for hydrogen production, the nutrient requirement in terms of COD:N:P was found to be 100:6:0.5, which was much higher than that for the methenogenic step for methane production under both thermophilic and mesophilic operations and for the acidogenic step for hydrogen production under mesophilic operation. 相似文献
18.
In this study, one macro-alga (Laminaria japonica) was used for fermentative hydrogen production by anaerobic mixed bacteria. The saccharification efficiency and hydrogen production by L. japonica with four different pretreatment methods, including heat, acid, alkaline and ultrasonic treatment, were investigated. The results showed that the saccharification efficiency from L. japonica that was pretreated with acid was the highest among the four methods. The saccharification efficiency for the total reducing sugars in the acid-pretreated L. japonica was 350.54 ± 19.89 mg/g (mean ± S.E.). The cumulative hydrogen production was 66.68 ± 5.68 mL/g from the heat-pretreated L. japonica, whereas that of L. japonica that was subjected to acid, alkaline, and ultrasonic pretreatment and the control was 43.65 ± 6.87 mL/g, 15.00 ± 3.89 mL/g, 23.56 ± 4.56 mL/g and 10.00 ± 1.21 mL/g, respectively. In addition, the effects of substrate concentration and initial pH on hydrogen production from heat-pretreated L. japonica were also analyzed. The results showed that the maximum hydrogen production was 83.45 ± 6.96 mL/g with a hydrogen concentration of approximately 28.4% from heat-pretreated L. japonica when the initial pH and substrate concentration were determined to be 6.0 and 2%, respectively. Heat pretreatment was the most effective method for increasing fermentative hydrogen production when L. japonica was used as the only substrate. 相似文献
19.
Thammanoon Sreethawong Suchawadee Chatsiriwatana Pramoch Rangsunvigit Sumaeth Chavadej 《International Journal of Hydrogen Energy》2010
In this work, hydrogen production from cassava wastewater using anaerobic sequencing batch reactors (ASBR) was investigated to determine the optimum number of cycles per day, chemical oxygen demand (COD) loading rate, and COD:N ratio. The system operated at a COD loading rate of 30 kg/m3d and 6 cycles per day provided maximum hydrogen production performance in terms of specific hydrogen production rate (SHPR) (388 ml H2/g VSS d or 3800 ml H2/l d) and hydrogen yield (186 ml H2/g COD removed). The effect of nitrogen supplementation was also studied by adding NH4HCO3 into the system at the COD:N ratios of 100:2.2, 100:3.3, and 100:4.4 under the COD loading rate of 30 kg/m3d and 6 cycles per day. The maximum SHPR and hydrogen yield of 524 ml H2/g VSS d (5680 ml H2/l d) and 438 ml H2/g COD removed, respectively, were obtained at the stoichiometric COD:N ratio of 100:2.2. An excess nitrogen was found to promote the productions of higher organic acids and ethanol, resulting in lowering hydrogen production efficiency. 相似文献
20.
The composition of media for thermophilic biohydrogen production from the enzymatic hydrolysate of cellulose fraction of sweet sorghum bagasse by Thermoanaerobacterium thermosaccharolyticum KKU19 were optimized in order to maximize the hydrogen production potential (Ps). Results from Plackett-Burman design indicated that FeSO4, CaCl2, NaHCO3, and MgCl2 had a significantly effect (P ≤ 0.05) on Ps. The optimum media composition obtained from the response surface methodology (RSM) with central composite design (CCD), using the hydrolysate at a total sugar concentration of 8.98 g/L, were (all in mg/L): FeSO4, 1454.65; MgCl2, 511.36; CaCl2, 278.62; and NaHCO3, 2186.41 in which the Ps of 2397 mL H2/L were obtained. Verification experiment using the optimum media composition in a continuous stirred tank reactor indicated a highly reproducible result in which the Ps of 2608 mL H2/L was achieved at a hydraulic retention time of 32 h. The results demonstrated that the media composition obtained from the batch experiment using RSM with CCD can be practically applied to continuously produce hydrogen from the hydrolysate with the least error. 相似文献