利用显微录像系统研究抗-Rop1Ps抗体对花粉管生长的影响

介绍一个简单的花粉萌发液循环装置,利用该循环装置可以在数小时内连续不间断地跟踪观察花粉管的生长状况。并介绍花粉粒显微注射的一些经验。利用该装置,在显微录像系统下研究抗Rop1Ps抗体对百合花粉管生长的影响。     

高真空光刻样品CCD显微观察装置

介绍了利用光场控制中性原子束制作微细图形的基本原理。针对具体的实验系统 ,设计并建立了一套用于高真空系统中原子光刻样品的 CCD显微观察装置 ,该装置以CCD相机实时采样通过计算机自动图像处理直接显示和输出样品的微结构信息。与传统装置相比 ,该装置在实验初期摸索实验工艺参数方面具有非常重要的作用。     

显微虫卵微机自动检测装置及虫卵快速检测算法

本文简要介绍了显微虫卵微机自动检测装置的硬件设计。详细介绍了基于该装置的绦虫卵快速检测算法。该算法根据绦虫卵图象特点，将传统目标检测步骤有机地融合在一起，使预处理和检测一次完成。在无虫卵情况下可快速扫描图象，提高了检测速度，同时也提高了检测率。实验结果证实了这些效果     

基于液滴透镜的手持式显微成像系统的研制

为了实现对昆虫显微成像,设计了一款可附着在手机镜头上的基于液滴透镜的手持式显微成像系统。利用Inventor机械设计软件设计一个小型装置,该装置主要由照明模块、成像模块、电源模块和升降对焦系统组成。分析了成像模块中不同面形液滴透镜的成像性能,并对该装置进行了全面的测试评估。研究表明,该系统能准确、快捷地对昆虫进行显微成...     

燃气蒸汽联合循环发电装置用煤气压缩机的设计

介绍了燃气蒸汽联合循环发电装置，并对该装置中的煤气压缩机的介质成分、选型、高低压缸、密封等设计思想进行了论述。该研究成果对冶金行业燃气蒸汽联合发电装置的应用具有促进作用。     

数字化进退针装置的设计与试验

针对细胞注射中微注射针刺膜速度慢和定位精度差等不足,研制一种基于惯性力与摩擦力交替作用的数字化进退针装置。阐述该装置工作机理,利用压电陶瓷低频振动产生的冲击式惯性力,克服摩擦力产生微进给。建立数字化进退针装置的动力学模型,利用数值方法求解其输入输出响应关系。该模型求解结果在性能试验得到了验证。在小鼠卵母细胞显微注射试验中,对数字化进退针装置进行了应用研究。研究结果表明：数字化进退针装置能够满足显微注射要求,其动力学模型是合理的。     

基于NRF24e1的无线通信网络

介绍了基于NRF24el的无线通信网络。本文利用单片无线收发芯片NRF24el搭建了无线收发装置，并且利用多个无线收发装置，采用令牌循环查询的模式组建了通信网络。实现网内数据透明传输。     

基于单片机的冷却装置漏油检测系统的研制

本文介绍一种多通道循环冷却装置漏油检测系统。该系统由单片机应用电路和超声波乳化装置组成。在应用电路中设计了满足用户需要的多路模拟开关进行多点数据采集，并采用了点阵式液晶显示屏，具有较强的显示功能。本文着重于单片机应用系统的介绍。     

细胞注射中数字化进退针装置的实验研究

针对微注射针刺膜速度慢和定位精度差等问题,研制了显微注射用数字化进退针装置。在可控脉冲序列驱动下,利用压电陶瓷低频振动产生的冲击惯性力驱动该装置来进行数字化进退操作。测试了数字化进退针装置样机的运动性能,在小鼠卵母细胞显微注射实验中对其进行了验证。结果表明:数字化进退针装置具有纳米量级进给精度,过程参数可控,可自动完成包括快速穿刺细胞膜、精确刺入细胞内指定注射位置和退针的整套显微操作。     

TM02D型串联式干气密封在重整循环氢气压缩机上的应用

介绍TM02D型串联式干气密封的结构和工作原理,探讨该密封装置在重整循环氢压缩机中的使用及维护。该套密封装置性能稳定可靠,可确保装置长周期平稳运行。     

Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination

Pollen allergens offer a dual perspective of study: some of them are considered key proteins for pollen physiology, but they are also able to trigger allergy symptoms in susceptible humans after coming in contact with their tissues. Profilin (Ole e 2 allergen) has been characterized, to some extent, as one of the major allergens from Olea europaea L. pollen, a highly allergenic species in the Mediterranean countries. In order to obtain clues regarding the biological role of this protein, we have analyzed both its cellular localization and the organization of actin throughout pollen hydration and early pollen tube germination. The localization of the cited proteins was visualized by confocal laser scanning microscopy immunofluorescence using different antibodies. Upon pollen hydration and pollen germination, a massive presence of profilin was detected close to the site of pollen tube emergence, forming a ring-like structure around the 'effective' apertural region. Profilin was also detected in the pollen exine of the germinating pollen grains and in the germination medium. After using a permeabilization-enhanced protocol for immunolocalization, profilin was also localized in the cytoplasm of the pollen tube, particularly at both the proximal and apical ends. Noticeable accumulations of actin were observed in the cytoplasm of the pollen tube; particularly, in both the apical region and the area immediately close to the aperture. Actin filaments were not observed, probably due to the need of further enhanced fixation procedures. The ultrastructural localization of profilin showed the presence of the protein in the cytoplasm of both the mature pollen grain and the pollen tube. The results shown here could be interpreted as signs of a massive dissociation of the actin-profilin complexes, mobilization of actin monomers, and therefore, an intense activity of the actin cytoskeleton. The extensive release of allergenic proteins from the pollen grain into the surrounding aqueous media, as described here for profilin, may help us to understand the mechanisms by which these allergens might come in contact with the human mucosa, therefore triggering the symptoms of allergy.     

油管与扶正机械手的接触碰撞仿真研究

油管摆动系统是修井作业机械化系统中的一部分,油管摆动系统中油管与扶正机械手间的接触碰撞将影响整个修井作业机械化系统的快速、安全及准确地运行。为研究扶正机械手承接件材料及节流阀开口大小对油管与扶正机械手接触碰撞特性的影响,根据油管摆动系统的工作原理和结构特点,利用三维软件SolidWorks和ADAMS软件建立了油管摆动系统的机液一体化虚拟样机。仿真分析了在扶正机械手承接件的两种材料和液压回路中节流阀3种开口大小下油管与扶正机械手接触碰撞特性,得到当承接件材料为橡胶及节流阀开口较小时油管与扶正机械手间具有接触碰撞力小、接触碰撞次数少的结论。     

The relationship between eccentric structure and super-lift device of all-terrain crane based on the overall stability

When the large all-terrain crane operated under ultra-high hoisting height, the stability of boom system became the key factor of the lifting capacity. The installation of eccentric structure and super-lift device could help to improve the stability of boom system. In order to achieve the best effect of growth, it was important to study the relationship between eccentric structure and super-lift device. The eigenvalue buckling analysis and geometric nonlinear buckling analysis were carried out by ANSYS for the combined boom system of 500 t all-terrain crane with tower jib when lifting load, dead weight, offset load, wind load and lifting rope force were considered. And then the influence rule of the combined relationship between the strut length, opening angle, amplitude variation angle of the eccentric structure and the strut length, opening angle, amplitude variation angle of the super-lift device on the overall stability of combined boom system was analyzed to obtain the optimum parameter combination of eccentric structure and super-lift device, which provides reference for engineering practice.

     

Rates of exocytosis and endocytosis in Arabidopsis root hairs and pollen tubes

Exocytosis and endocytosis are pivotal in many biological processes, but remain difficult to quantify. Here we combine a new algorithm for estimating vesicle size with a detailed morphological analysis of tip-growing cells, in which exocytosis is highly localized and therefore more readily quantified. Cell preservation was rendered as life-like as possible by rapid freezing. This allowed us to produce the first estimates of exocytosis rates in the root hairs and pollen tubes of the model plant Arabidopsis. To quantify exocytosis and endocytosis rates during cell growth, we measured the diameter of vesicles located in the tips of Arabidopsis root hairs and pollen tubes and the widths of cell walls and the cell lumen in longitudinal thin transmission electron microscopic sections. In addition, we measured growth velocities of Arabidopsis root hairs and pollen tubes, using video microscopy. The number of exocytotic vesicles required for cell wall expansion, and the amount of excess membrane inserted into the plasma membrane to be internalized, were estimated from the values that were obtained. The amount of excess membrane that is inserted into the plasma membrane during cell growth was estimated as 86.7% in root hairs and 79% in pollen tubes. This membrane has to be recycled by endocytosis. From counting of the total number of vesicles that is present in thin EM sections through the pollen tube tip, we estimated the average number of vesicles that is present in the tip of pollen tubes. By calculating the total amount of membrane and cell wall material that is required for continued cell growth, assuming that all vesicles are exocytotic, we estimated that pollen tubes continue to grow for 33 s when delivery of vesicles to the tip is inhibited. We arrested vesicle delivery to the tip by application of cytochalasin D. After cytochalasin D application, pollen tubes continued to grow for 30-40 s, which is in the same range as the estimated value of 33 s and shows that in this time frame, the availability of exocytotic vesicles is not a limiting factor.     

基于ATmega8单片机的仪表指针驱动控制研究

仪表指针的驱动控制在仿真培训装置的开发设计中有重要应用。以弹簧管压力表的指针驱动为例,研究了基于ATmega8单片机控制VID29步进电机的驱动控制实现方案,设计制作了硬件电路,采用模块化方法编写了系统软件,在锅炉仿真培训模拟机上的使用效果表明该方案响应快、定位准、稳定可靠,对于同类传统仪表的电气化改装及数字式仪表的设计具有一定参考价值。     

高速矫直机防甩装置的开发与研制

高速无缝钢管矫直机在生产实际中一般用来矫直较大尺寸范围的管材,但当遇到管径较小的管材时,管材甩动非常严重.为使该设备能在矫直要求的尺寸范围内正常工作,开发并研制了矫直机防甩装置.经过商用软件Marc对不同规格的管材进行模拟分析,确定了防甩装置的工艺参数,如辊形、防甩辊安装位置等,分析了在管材甩动过程中不同状态的管端位移情况,确定了高速矫直机的防甩装置结构.经过实际应用可以看出,其遏止甩动效果明显,可以应用于矫直范围宽的生产工艺中.     

工程机械余热驱动冷管型吸附空调的运行特性

设计建造一种利用工程车余热驱动的冷管型组合式吸附空调系统,建立相应的理论模型和性能分析指标,在此基础上进行空调系统的运行性能试验与分析。在一定的外部环境工况和循环半周期为30 min条件下,当 吸附床采用风冷时,空调器最大制冷降温幅度为13.0 ℃,最大制冷功率为1.10 kW,平均制冷功率842 W, 性能系数e c=0.168,单位质量吸附剂制冷功率Ps=88.2 W·kg–1。当吸附床改进冷却方式后,空调器最大制冷 降温幅度为14.0 ℃,比风冷状态下提高1.0 ℃,最大制冷功率达1.21 kW,平均制冷功率926 W,e c=0.177,Ps= 97.0 W·kg–1。可得出以下结论,吸附床冷却方式改进后系统e c提高约5.4%,最大制冷功率、平均制冷功率和Ps各提高将近10%。     

超低速运动装置设计分析

单晶炉提拉装置是提拉法生长晶体的关键设备,由于激光晶体生长机理决定了其生长速度非常缓慢。提拉装置要求长期运行在超低速的状态下,针对运动系统的震动、晃动、摩擦、爬行等都会影响速度的稳定性,采用交流伺服技术对激光单晶炉的提拉装置进行了全新改造设计,并对其精度进行了综合分析。通过采用变增益的PID控制方法长期运行的实验数据表明,该装置运行精度高,稳定性好,适合生长大尺寸激光晶体,同时设计采用通用零件,满足大规模工业化生产的需求。