葵花籽粕中绿原酸分离及含量分析

本文通过研究以聚酰胺薄膜为固定相、体积分数为36％的乙酸溶液为流动相,确定薄层色谱分离测定葵花籽粕中绿原酸的方法。该方法容易操作,准确度和灵敏度高,为葵花籽粕中绿原酸的测定提供了理论依据。     

葵花籽粕中绿原酸分离及含量分析

本文通过研究以聚酰胺薄膜为固定相、体积分数为36％的乙酸溶液为流动相，确定薄层色谱分离测定葵花籽粕中绿原酸的方法。该方法容易操作，准确度和灵敏度高，为葵花籽粕中绿原酸的测定提供了理论依据。     

复方西羚解毒胶囊质量标准研究

目的研究复方西羚解毒胶囊的质量标准。方法采用薄层色谱法(TLC)对其制剂处方中的冰片、薄荷脑、连翘、甘草等进行定性鉴别;采用高效相色谱法(HPLC)测定复方西羚解毒胶囊中绿原酸含量。结果薄层色谱鉴别表明,样品分离度好,斑点清晰,且阴性对照无干扰,绿原酸浓度在0.01~0.12 mg/m L(r=0.9999)范围内呈良好线性关系,平均回收率为97.69%,RSD为0.94%(n=6)。结论该标准操作简便,专属性、稳定性、重复性良好,适用于复方西羚解毒胶囊的质量控制。     

RP-HPLC测定咽清颗粒中绿原酸含量

目的 建立测定咽清颗粒中绿原酸含量的方法.方法 以反相C18色谱柱为固定相(250 mm×4.60 mm,5 μm),乙腈-0.4%磷酸溶液(12:88)为流动相,流速1.0 mL·min-1,检测波长327 nm.结果 绿原酸进样量10～60 μg·mL-1范围内与峰面积积分值旱良好的线性关系(r=0.999 9);平均回收率97.17%,RSD=1.73%(n=9).结论 本方法简便、可靠、准确,可用于测定咽清颗粒中绿原酸的含量.     

高效液相色谱法测定蓝莓叶中绿原酸的含量

采用高效液相色谱法测定蓝莓叶中绿原酸的含量。所用色谱柱为Eclipse XDB C18(250 mm×4.6 mm,2.5μm),流动相为乙腈-0.1%磷酸(体积比为13:87),检测波长327 nm。当绿原酸质量浓度为6~96μg/m L时,以绿原酸对照品溶液的峰面积比为纵坐标(Y)、绿原酸的质量浓度为横坐标(X)进行线性回归,二者呈良好的线性关系,线性方程为:Y=74.15X-432.3,R2=0.999,平均回收率为99.98%,RSD=0.506%(n=6)。按照以上色谱方法检测了3种蓝莓叶中绿原酸的含量,并与蓝莓果实中绿原酸的含量比较,结果显示,3种蓝莓叶中的绿原酸含量显著高于对应品种果实中的绿原酸含量(P0.05)。     

克痫胶囊的质量标准研究

目的 建立克痫胶囊的质量标准.方法 用薄层色谱法定性鉴别克痫胶囊中钩藤、冰片,用紫外分光光度法测定胆酸含量.结果 在薄层色谱中检出钩藤和冰片,胆酸在0.1～0.5 mg范围内具有良好的线性关系,r=0.9999,平均回收率98.95％,RSD=2.25％.结论 本方法简便可行,专属性强,重复性好,可用于克痫胶囊的质量控制.     

高效液相色谱-串联质谱法测定饮料中7种绿原酸类物质

目的：建立了一种高效液相色谱-串联质谱法(High performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)同时快速、准确测定饮料中7种绿原酸类物质含量的方法。方法：研究不同柱温、流速、色谱柱、提取溶剂、超声时间等对7种绿原酸类物质测定的影响,最终确定试样经90%甲醇水溶液超声提取,采用RP18(2.1 mm×100 mm,1.7μm)色谱柱分离,HPLC-MS/MS同时测定新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、1,5-二咖啡酰奎宁酸成分含量。结果：对HPLC-MS/MS检测7种成分的检测条件优化和方法学考察,包括色谱条件。结论：该检测方法简便、准确、可靠,线性关系、精密度、重复性、稳定性、加样回收率均符合要求,可用于饮料中7种绿原酸类成分的定量分析。     

清热化痰合剂质量标准的研究

目的 制定清热化痰合剂的质量标准.方法 采用薄层色谱法(TLC)定性鉴别方中的麻黄和浙贝母.采用高效液相色谱法(HPLC)测定绿原酸的含量,色谱柱为Unitary C18(250 mm×4.6 mm,5μm);流动相为0.1％醋酸溶液-乙腈,梯度洗脱;流速1 ml/min,进样量10μl,柱温30℃,检测波长为327 ...     

RP-HPLC测定咽清颗粒中绿原酸含量

目的建立测定咽清颗粒中绿原酸含量的方法。方法以反相C18色谱柱为固定相(250mm×4.60mm,5μm),乙腈-0.4%磷酸溶液(12∶88)为流动相,流速1.0mL·min-1,检测波长327nm。结果绿原酸进样量10～60μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率97.17%,RSD=1.73%(n=9)。结论本方法简便、可靠、准确,可用于测定咽清颗粒中绿原酸的含量。     

绿茶中药用绿原酸成分分析

本论文建立了以硅胶G60作为薄层固定相，层析分离（TLC）绿茶中绿原酸，使用紫外分光光度法（UV）定量分析绿茶中绿原酸含量的TLC－UV法。对绿茶中的绿原酸的提取条件、点样量及方法的精密度、准确度等进行了考察。并对绿茶（20010312粤春茶叶厂生产）中绿原酸含量进行了测定，测定结果显示每克绿花中含绿原酸9.12mg/g。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the