共查询到18条相似文献,搜索用时 62 毫秒
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通过在液体培养基中添加不同浓度的吲哚乙酸,研究吲哚乙酸对冬虫夏草生物量、胞外多糖产量及其抗氧化性能的影响。结果表明:适宜浓度的吲哚乙酸可以提高冬虫夏草的生物量和胞外多糖产量,并且随吲哚乙酸浓度的增加二者均呈先增后降的变化趋势,当浓度为2.5μg/m L时,生物量和胞外多糖产量均达到最高,分别为18.3g/L和2.9g/L,较不添加吲哚乙酸分别增加了6.3%和15.4%;同时,适宜浓度的吲哚乙酸还可以提高冬虫夏草胞外多糖的抗氧化性能,在1.0μg/m L和2.5μg/m L时,胞外多糖对羟基自由基和DPPH的清除率分别达到最高,为58.9%和56.6%。 相似文献
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灰树花是一种营养丰富的食、药两用真菌 .灰树花胞外多糖是一种具有生理活性的真菌多糖 .作者研究了营养条件对灰树花产胞外多糖的影响 ,确定了合理的初始碳源、氮源、无机离子、生长因子的质量浓度 .进一步的正交试验表明 ,灰树花产胞外多糖较佳的培养基组合为 :葡萄糖 4 0g/L ,蛋白胨 5g/L、磷酸二氢钾 4 g/L ,硫酸镁 2 g/L、玉米浆 2 0 g/L . 相似文献
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以冬虫夏草胞外多糖为研究对象,通过凝胶渗透色谱分析酸水解多糖的分子质量、分子质量降解率(MWDR)和多分散指数(MW/Mn)的变化规律,并在此基础上探讨酸水解动力学。结果表明:酸水解可得到高分子质量和低分子质量两个多糖组分,高分子质量多糖组分的含量、分子质量和MW/Mn均随酸解时间的增加而减小,而MWDR呈线性增加趋势。低分子质量多糖组分含量随酸水解时间的增加而增加,分子质量基本维持在3.0kD;MWDR在0.5h内呈线性增加趋势,大于0.5h后基本维持在0.25。胞外多糖的酸水解模式为中心链断裂,符合假一级反应动力学方程,反应速率常数为3.24×10-2min-1,远小于随机链断裂的反应速率常数。 相似文献
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冬虫夏草多糖的研究进展 总被引:4,自引:2,他引:4
多糖是冬虫夏草所含活性物质中最重要最丰富的类群之一,本文综述了冬虫夏草多糖的提取、结构和药理学活性的研究进展,并提出了目前冬虫夏草多糖研究中存在的问题,为冬虫夏草多糖的进一步研究开发提供依据。 相似文献
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Cordyceps sinensis (Berk.) Sacc., which is a part of traditional Chinese medicine, has been used as a tonic food and herbal medicine in Asia for centuries. C. sinensis is sold as powders, and the quality is often questionable. To confirm the quality of C. sinensis, products were purchased from different supermarkets in Taiwan. Product samples were analyzed using PCR-single-stranded conformation polymorphism (PCR-SSCP) assay based on internal transcribed spacer 2 (ITS2). PCR-SSCP patterns distinguished ten samples into six groups, but no sample was grouped with representative C. sinensis (rCS). Only two of the ten C. sinensis functional ingredient samples (CSFFMS) tested CB and ST, were similar to the reference product of C. sinensis. This in consistent result could be caused by poor fermentation control process, contamination of original marketing samples or adultration. Therefore, the use of PCR-SSCP could be used for species differentiation or the detection of mutations based on ITS2 region in C. sinensis. The method represents a potential novel means for the differentiation of Cordyceps herbal medicines/functional ingredients and could be used as a quality control in the Cordyceps fermentation industry. 相似文献
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Hsiao-Che Kuo Yong-Lin Su Huey-Lang Yang I-Ching Huang Tzong-Yueh Chen 《Food Biotechnology》2006,20(2):161-170
Cordyceps sinensis (Berk.) Sacc., which is a part of traditional Chinese medicine, has been used as a tonic food and herbal medicine in Asia for centuries. C. sinensis is sold as powders, and the quality is often questionable. To confirm the quality of C. sinensis, products were purchased from different supermarkets in Taiwan. Product samples were analyzed using PCR-single-stranded conformation polymorphism (PCR-SSCP) assay based on internal transcribed spacer 2 (ITS2). PCR-SSCP patterns distinguished ten samples into six groups, but no sample was grouped with representative C. sinensis (rCS). Only two of the ten C. sinensis functional ingredient samples (CSFFMS) tested CB and ST, were similar to the reference product of C. sinensis. This in consistent result could be caused by poor fermentation control process, contamination of original marketing samples or adultration. Therefore, the use of PCR-SSCP could be used for species differentiation or the detection of mutations based on ITS2 region in C. sinensis. The method represents a potential novel means for the differentiation of Cordyceps herbal medicines/functional ingredients and could be used as a quality control in the Cordyceps fermentation industry. 相似文献
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根据对白砂糖、柠檬酸的阈值测定结果,研究了以虫草和茶叶为原料的饮料的风味。正交试验结果表明:以茶汤50ml、虫草浸提液22.5ml的添加量,配制的虫草茶饮料风味最佳。 相似文献
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Microwave‐Assisted Extraction,Chemical Structures,and Chain Conformation of Polysaccharides from a Novel Cordyceps Sinensis Fungus UM01 下载免费PDF全文
Kit‐Leong Cheong Lan‐Ying Wang Ding‐Tao Wu De‐Jun Hu Jing Zhao Shao‐Ping Li 《Journal of food science》2016,81(9):C2167-C2174
Cordyceps sinensis is a well‐known tonic food with broad medicinal properties. The aim of the present study was to investigate the optimization of microwave‐assisted extraction (MAE) and characterize chemical structures and chain conformation of polysaccharides from a novel C. sinensis fungus UM01. Ion‐exchange and gel filtration chromatography were used to purify the polysaccharides. The chemical structure of purified polysaccharide was determined through gas chromatography‐mass spectrometry. Moreover, high performance size exclusion chromatography combined with refractive index detector and multiangle laser light scattering were conducted to analyze the molecular weight (Mw) and chain conformation of purified polysaccharide. Based on the orthogonal design L9, optimal MAE conditions could be obtained through 1300 W of microwave power, with a 5‐min irradiation time at a solid to water ratio of 1:60, generating the highest extraction yield of 6.20%. Subsequently, the polysaccharide UM01‐S1 was purified. The UM01‐S1 is a glucan‐type polysaccharide with a (1→4)‐β‐d ‐glucosyl backbone and branching points located at O‐3 of Glcp with a terminal‐d ‐Glcp. The Mw, radius of gyration (Rg) and hydrodynamic radius (Rh) of UM01‐S1 were determined as 5.442 × 106 Da, 21.8 and 20.2 nm, respectively. Using the polymer solution theory, the exponent (ν) value of the power law function was calculated as 0.38, and the shape factor (ρ = Rg/Rh) was 1.079, indicating that UM01‐S1 has a sphere‐like conformation with a branched structure in an aqueous solution. These results provide fundamental information for the future application of polysaccharides from cultured C. sinensis in health and functional food area. 相似文献