Production of Microbiologically Stable Apple Juice by Metallic Membrane Ultrafiltration

The potential for production of microbiologically stable apple juice by metallic membrane ultrafiltration was assessed. Thermal sterilization treatments caused no detectable changes in membrane performance. Sterile membranes were challenged with approximately 3 × 10⁶ colony forming units per gram of each of three organisms, Pseudomonas diminuta, Bacillus coagulans, and Saccharomyces cerevisiae, suspended in apple puree or peptone. Reductions achieved by ultrafiltration of peptone at operating pressure were 5.1, 7.6, and >9.0 logs for the three organisms, respectively. Reductions of organisms in apple puree were at least 1.5 logs greater. Metallic membranes eliminated yeasts and molds from juice. Apple juice produced from uninoculated apple puree was stable at 20–25°C for 1 yr.     

Evaluation of hybrid pressure-driven and osmotically-driven membrane process for non-thermal production of apple juice concentrate

This study investigates the potential of hybrid membrane processes including microfiltration (MF), ultrafiltration (UF) and forward osmosis (FO) for non-thermal concentration of apple juice. The process performance and characteristics (physicochemical properties, nutritional and aroma components and microbiological quality) of apple juice were studied. The clarity of apple juice was significantly promoted as pore size of membrane reduced. MF and UF can also ensure microbiological safety in pre-treated apple juice. According to its efficiency of filtration as well as performance of simultaneous clarification and cold-sterilization, 0.22 μm MF membrane was identified as the optimal membrane for the pre-treatment. The pre-treated apple juice can be concentrated up to 65°Brix by subsequent single stage FO. FO retained nutritional and volatile compounds of apple juice while significant reductions were found in the juice concentrated by vacuum evaporation. Hybrid MF-FO can be a promising non-thermal technology to produce apple juice concentrate with high quality.     

Optimization of Apple Juice Production by Single Pass Metallic Membrane Ultrafiltration

Apple puree was treated with various amounts of commercial liquefaction enzyme (0%– 0.066%) for 2 hr at 50°C. To produce clarified juice purees were pumped through a metallic oxide membrane ultrafiltration system, consisting of 3.27 ft² (0.304 m²) of membrane coated internally onto a l–1/4 inch (i.d.) × 10 ft (3.12 cm × 3.04 m) sintered stainless steel tubing. All enzyme treatments reduced viscosity 70–80% within 1 hr but steady state flux increased with higher concentrations of enzyme. Alcohol insoluble solids (AIS) and total pectins of the apple puree after enzyme treatment decreased as enzyme concentration increased to 0.044%. A large scale system, l–1/4 inch (i.d.) × 120 ft (3.12 cm × 36.48 m), was operated at 86% juice yield with minimal pressure drops as predicted by a mathematical model.     

Effect of Ultrafiltration Combined with High-Pressure Processing on Safety and Quality Features of Fresh Apple Juice

Ultrafiltration (UF, 0.05 μm) with a ceramic membrane was combined with high-pressure processing (HPP) at 500 MPa/6 min and high-temperature short time (HTST) at 110 °C/8.6 s to process fresh apple juice. The aim of this study was to compare the effect of UF + HPP and UF + HTST on quality features of fresh apple juice and analyze the quality changes of the juice treated by UF + HPP and stored during 60 days at 4 °C. Applying UF, total plate count (TPC) and yeasts and molds (Y&M) significantly decreased by 0.29 and 0.28 log cycle, total phenols and ascorbic acid decreased by 33.50 and 26.52 %, and antioxidant capacity, using the DPPH and FRAP assay, significantly decreased by 26.40 and 25.37 %. Meanwhile, the juice clarity was 99.75?±?0.07 % and seven aroma compounds were changed. TPC and Y&M in juices treated by UF + HPP and UF + HTST were <1 log cycle. When compared to the juice treated by UF + HTST, the juice treated by UF + HPP showed lower browning degree and higher total phenols and clarity, and retained seven main volatile aroma compounds. Fresh apple juice processed by UF + HPP was microbiologically safe (TPC <1.8 log cycles and Y&M <1 log cycle) during 60 days of storage at 4 °C. The first-order model was a suitable model for all quality parameters of refrigerated fresh apple juice; however, rate constant k of first-order model was between ?0.0157 and 0.0350, showing the quality features of the refrigerated juice was stable.     

Direct Use of Apple Pomace in Bakery Products

DRUM-DRIED APPLE POMACE (DDAP), an edible by-product from single pass metallic membrane ultrafiltration of apple puree, was examined for composition and utilization as a source of sugar and dietary fiber in bakery products. Total dietary fiber (TDF) of freezc-dricd apple pomace (FDAP) and DDAP was 35.29% and 33.24%, respectively. Total sugar of the apple pomace concentrate (APC), FDAP, and DDAP was 54.34, 46.26, and 36.71%, respectively. Sensory evaluation demonstrated that the experimental muffins with 50% (w/w) of the plain wheat bran substituted by powdered apple pomace were significantly (p < 0.01) more desirable than the control bran muffins. In addition, experimental moon cookies with flaked apple pomace substituted for 40% (w/w) of the all-purpose flour in the crust and 40% (w/w) of the quick-cooking oats in the filling were significantly (p < 0.01) more desirable than the control moon cookies.     

Clarification of Apple Juice by Hollow Fiber Ultrafiltration: Fluxes and Retention of Odor-Active Volatiles

Fresh apple juice was clarified in a pilot scale ultrafiltration (UF) unit, with membranes made of polysulfone and polyamide and plate and frame and vacuum drum filters. Flux of apple juice (L/m² hr) vs UF transmembrane pressure data showed an optimum at a pressure of about 140 kPa. Retention of odor-active volatiles was highest in plate and frame filtered apple juice and lowest in vacuum drum filtered juice; the retention of odor-active volatiles in UF juice was intermediate to the two traditional filtration methods. Retention of odor-active volatiles in the permeate of a polyamide membrane was higher than that of a polysulfone membrane.     

COLOR, POLYPHENOLOXIDASE, AND SENSORY CHANGES IN BANANA JUICE AS AFFECTED BY HEAT AND ULTRAFILTRATION

A study was conducted to determine the effects of banana maturity (less mature; maturity stage 3, more mature; maturity stage 7), heat treatment of banana puree, heat pasteurization of juice, and ultrafiltration (UF) of juice on color, polyphenoloxidase (PPO) activity, and flavor of puree and juice. Heating puree to 90C inactivated PPO and reduced browning in puree and juice, especially in puree from more mature bananas which had greater PPO activity. PPO activity decreased considerably during cold-setting and filtration of juice, and was not detectable in unheated, pad-filtered juice from less mature bananas. UF of juice through a 10,000 mwco membrance, but not a 30,000, removed all PPO activity and reduced juice browning. Pasteurization of juice (hot fill at 90C) increased browning, but not as much as PPO activity in the puree. Pasteurization had no effect on juice flavor if the puree had been heated to 90C previously as puree, but altered the flavor (i. e., more cooked banana flavor) of juice that had not been previously heated to 90C. Pasteurized juice that had not been heated to 90C as puree had similar flavor (no significant difference) as pasteurized juice that had been heated to 90C as puree.     

Processing and analytical characterisation of pulp-enriched cloudy apple juices

Pulp-enriched cloudy apple juices were manufactured from two apple varieties by blending of apple puree with natural cloudy apple juice in order to enrich bioactive secondary plant substances. Finishing of the puree with a 0.6-mm mesh screen and final product homogenisation revealed as the optimum processing technology for the novel 100% fruit beverage. The presence of large particles originating from the puree prevented long-term cloud stability, but due to the proportion of cloud-stable juice a complete phase separation did not occur. Optimal drinkability was achieved at a viscosity of 11.5 mPa s. Polyphenol compositions and concentrations were determined by means of RP-HPLC/PDA. The results ranged from 109 to 610 mg/l. All samples showed the typical polyphenol pattern of apples with dominating hydroxycinnamic acids followed by flavanols and flavonols. The puree addition to the cloudy apple juices increased the polyphenol concentrations by average 100%. Relatively, the highest increase could be observed for dimeric procyanidins. Different technological variants did not affect significantly the polyphenol concentrations. The dietary fibre contents of the pulp-enriched cloudy apple juices ranged from 5.8 to 9.4 g/l.     

混浊苹果汁加工过程中的酶促褐变及其防止的研究

将几种可用于食品的防褐变剂在苹果破碎时加入,考察了它们对苹果浆和苹果鲜榨汁的防褐变效果及它们之间的协同作用。半胱氨酸、抗坏血酸、曲酸、氯化钠和氯化钙都是较好的防褐变剂。在所测试条件下,抗褐变的能力(褐变抑制率)为:0.1％的抗坏血酸>0.1％的偏重亚硫酸钠>0.1％的曲酸>0.1％的氯化钙>0.005％半胱氨酸>0.1％氯化钠。它们抗褐变能力都表现为浓度依赖型,特别是抗坏血酸。柠檬酸在所测试条件下没有抗褐变效果。0.044％NaCl 0.0125％Vc的组合较经济且可使果汁40min不变色。果浆脱气可减缓其褐变;减小果汁及果浆与空气的接触面也可防止褐变。     

APPLE JUICE QUALITY AS INFLUENCED BY ULTRAFILTRATION

Objective quality of ultrafiltered (UF) apple juice compared favorably to apple juice filtered by conventional plate and frame filtration (PF). Agtron red and green values were higher for the UF juice, indicating a lighter colored product. Agtron green values were lower after 4 months of storage and compared well to juice from conventional methods over the remaining 8 months of storage. Green color of the UF juice remained steady at storage temperatures of either 1° or 21°C and was equal to the color values of the conventional juice stored at 1°, 21° or 32°. Clarity of the UF juice was excellent and remained stable over the entire storage period at either 1 or 21° with only a slight drop in clarity at 32°. UF juice was rated significantly lower in sensory flavor than PF juice. Sensory color of the UF juice was not rated as high as the PF juice at the start of the study, but as time in storage progressed sensory color improved. Initial panel reaction to UF apple juice was that the UF juice was too light in color and watery in flavor.     

混浊苹果汁加工过程中的的酶促褐变及其防止的研究

将几种可用于食品的防褐变剂在苹果破碎时加入,考察了它们对苹果浆和苹果鲜榨汁的防褐变效果及它们之间的协同作用.半胱氨酸、抗坏血酸、曲酸、氯化钠和氯化钙都是较好的防褐变剂.在所测试条件下,抗褐变的能力(褐变抑制率)为0.1%的抗坏血酸＞0.1%的偏重亚硫酸钠＞0.1%的曲酸＞0.1%的氯化钙＞0.005%半胱氨酸＞0.1%氯化钠.它们抗褐变能力都表现为浓度依赖型,特别是抗坏血酸.柠檬酸在所测试条件下没有抗褐变效果.0.044%NaCl+0.0125%Vc的组合较经济且可使果汁40min不变色.果浆脱气可减缓其褐变;减小果汁及果浆与空气的接触面也可防止褐变.     

Effect of pectinase treatment on extraction of antioxidant phenols from pomace,for the production of puree-enriched cloudy apple juices

Effects of pomace maceration on yield, turbidity, cloud stability, composition of phenolics, antioxidant activity and colour properties were studied, to evaluate the potential applicability of enzyme preparations in puree-enriched cloudy apple juice production. The yield of mixed juice and puree from pomace obtained in the enzymatic processing of apple ranged from 92.3% to 95.3%, significantly higher than the yield from the control without enzymatic pomace treatment (81.8%). Higher turbidity was obtained upon pomace treatment with Pectinex XXL and Pectinex Ultra SPL enzymes. The total content of phenolic compounds in apple pomace was higher than in raw juices (1520 mg/kg and 441 mg/L, respectively). The total polyphenol yields were higher in juices treated with Pectinex AFP L-4, Pectinex Yield Mash and Pectinex XXL, as compared to the control treatment. During 6 months of storage, a significant change was observed in the content of polyphenols, especially in procyanidin fractions.     

Optimization and Validation of a Method Without Alkaline Clean-Up for Patulin Analysis on Apple Puree Agar Medium (APAM) and Apple Products

A sensitive high-performance liquid chromatography-UV (HPLC-UV) method, based on the Association of Analytical Communities (AOAC) Official method 2000.02, was developed and validated for the high-throughput analysis of patulin in in vitro experiments on apple puree agar medium (APAM). The importance of repeating the ethyl acetate extraction step at liquid-liquid extraction (LLE) was examined, as well as the extent of patulin degradation during the sodium carbonate clean-up. In addition to this alkaline clean-up, the efficiency of using an Oasis HLB or C₁₈ cartridge as solid-phase extraction (SPE) clean-up was compared. This resulted in a two-step ethyl acetate LLE, followed by an Oasis HLB SPE clean-up, without alkaline clean-up conditions. The method was fully validated for APAM, cloudy apple juice, and apple puree. Average patulin recoveries at levels of 100, 500, and 1000 μg kg^?1 of APAM varied between 95 and 113 % over 3 independent days, with an interday precision (RSD_R) of 5 to 10 %. Recovery experiments carried out with the spiked apple juice (at 50 μg kg^?1) and apple puree (10 μg kg^?1) showed average recovery rates laying between 80–101 % (RSD_R?=?12 %) and 77–100 % (RSD_R?=?9 %), respectively. This method offered a detection limit of 3–4 μg kg^?1 and a quantification limit of 5–8 μg kg^?1 for APAM, apple juice, and puree.     

Improved Ultrafiltration for Color Reduction and Stabilization of Apple Juice

Polyphenols that are responsible for haze-formation and browning during storage of clear apple juice and concentrates could be selectively removed by an ultrafiltration process using membranes of polyether sulfone and polyvinylpyrrolidone. Results were compared with those from commercial UF membranes made out of regenerated cellulose acetate. The effects of laccase treatment on removal of polyphenols and color in apple juice were also investigated. Custom membranes were effective to reduce the amount of polyphenols. A remarkable desired color removal of apple juice could also be achieved using these membranes. Resulting products were stable in color and clarity at 50°C up to 6 wk. Laccase treatment increased the percentage removal of polyphenols from apple juices. However, laccase treated samples were more susceptible to coloration and haze-formation during storage.     

破碎时蒸汽热处理对浑浊苹果汁色泽及浑浊稳定性的影响

苹果破碎时 ,蒸汽热处理使果浆温度在 80s内达 92℃以上 ,不但改善了浑浊苹果汁色泽且可增强果汁的浑浊稳定性。果汁的颜色明显受果浆所达到的最高温度影响。苹果浆温度达92℃和 95℃虽然VC 损失较多 ,但如果果汁的总酚损失较小 ,则有好的色泽稳定性。苹果破碎过程中发生的褐变主要为酶促褐变 ,但热处理温度高于 98℃ ,非酶褐变会变得显著。果汁的混浊稳定性受果汁中悬浮颗粒的大小、果汁的粘度影响。热处理增加了果胶的聚合度 ,促进果胶从果浆中的释放 ,从而增加了果汁的粘度。热还处理可减小果汁中悬浮颗浊的大小。     

Preparation of Passion Fruit Puree by Flash Vacuum-Expansion

ABSTRACT Purple passion fruit were processed by flash vacuum‐expansion in comparison with a single‐strength juice. A puree was obtained with about 50%/fruit weight yield, which is 2‐fold that obtained for the reference juice. Color and cell‐wall polysaccharides of the products were analyzed, and their rheological properties were investigated. The red‐purple puree was enriched in anthocyanins and alcohol‐insoluble residue. The puree had higher consistency and viscosity, which was related to its alcohol‐insoluble residue and starch contents.     

A Fast and Reliable UHPLC-PDA Method for Determination of Patulin in Apple Food Products Using QuEChERS Extraction

A fast and reliable method for the quantification of patulin using ultra high performance liquid chromatography coupled to a photodiode array detector was developed and validated for the analysis of several apple-based foodstuffs. Sample preparation was based on the QuEChERS procedure. Samples were extracted with acetonitrile and partitioned with a mixture of sodium citrate, NaCl, and Mg₂SO₄. The cleanup step was performed using dispersive SPE mixed with Mg₂SO₄ and PSA. This step was carried out twice in order to improve chromatographic results. The method was validated in spiked cloudy apple juice, apple puree, apple yogurt, beer with apple juice, and cider and applied to 24 commercial samples. The limits of detection and limits of quantification were ≥0.4 and ≥2 ng/g, respectively. Both were below the maximum legal limit established by the European Union. Recoveries for all the matrices were between 78.4 and 94.7 % while relative standard deviations were between 3.8 and 10.4 %. Patulin was detected on four apple juices from concentrate, one cloudy apple juice from an eco-store, and one beer. Nevertheless, the highest concentration found was 25.4 ng/g.     

Moisture sorption characteristics and glass transition temperature of apple puree powder

The aim of this study was to characterise the influence of different foam‐mat‐drying methods on the moisture sorption characteristics and glass transition temperatures of apple puree powder. Apple puree was foamed with the addition of 2.5% egg albumin and 0.5% methylcellulose. Convective air‐drying and microwave‐drying techniques were used. Also foamed puree with and without maltodextrin (6% or 15% w/w) was freeze‐dried. Moisture equilibrium data of powders were determined by using a static desiccator method in a water activity range of 0.0–0.903. Modulated differential scanning calorimetry (MDSC) was used to obtain the glass transition temperature. No effect of drying method on sorption properties and glass transition temperatures of apple puree powders was observed. The addition of maltodextrin to the apple puree caused an increase in T_g by 10–30 °C depending on the amount of incorporated additive. Addition of maltodextrin significantly reduced the hygroscopicity of apple puree powders.     

Solute and Enzyme Recoveries in Apple Juice Clarification using Ultrafiltration

Enzyme treated apple juices, obtained after pressing on a commercial processing line, were clarified by UF using a plate and frame system. The 50,000 molecular weight cut-off membranes operated at 50°C and 5 bar pressure yielded average recovery of 99.6% and 99.3% soluble solids and titratable acids, respectively, in the permeate and rejected 36.3% of the residual pectinase activity which was available for possible reuse. Proper membrane cleaning to maintain high flux was very important in UF clarification; a 1% Ultrasil solution was more efficient than a 0.1% NaOH solution and reduced need for centrifugation and/ or vacuum filtration prior to UF.     

Microfiltration and Ultrafiltration Ceramic Membranes for Apple Juice Clarification

Depectinized control (CTJ) and ascorbic acid treated (AAJ) apple juices were filtered through microfiltration (MF, 0.2 m) and ultrafiltration (UF, 0.02 m) ceramic tubular membranes. Under optimal conditions (8 m/s, 414 kPa and 50°C), the UF membrane resulted in higher steady state flux and less fouling than the MF membrane for both juices. AAJ produced by addition of AA at milling had lower flux for both MF and UF. The chemical, physical and sensory properties of apple juices from MF and UF membranes were similar and changes during storage were comparable.