从脱脂豆粕中提取大豆低聚糖的工艺研究

以脱脂豆粕为原料,经过碱液浸提,酸沉淀蛋白,活性炭脱色,离子交换脱盐,减压蒸馏浓缩,乙醇沉淀,离心,烘干等工序,提取并分离大豆低聚糖产品。主要对大豆低聚糖的浸提工艺、脱色工艺和脱盐工艺进行优化,确定各工艺的最好条件。大豆低聚糖浸提的最佳工艺条件为:1%Na_2CO_3溶液,浸提温度55℃,浸提时间1.5 h;大豆低聚糖脱色的最佳工艺条件为:活性炭用量1.5%,pH 3,脱色温度50℃,脱色时间30 min;大豆低聚糖脱盐的最佳流速为0.05 cm~3糖液/(cm~3树脂柱·min)。采用此工艺可从10 g脱脂豆粕中得到0.565 g大豆低聚糖产品。     

从大豆糖蜜中分离低聚糖的研究

论述了从大豆糖蜜中分离大豆低聚糖的方法。大豆糖蜜经絮凝沉淀分离蛋白后得到大豆粗糖浆,再经脱色、浓缩得到高浓度大豆低聚糖浆。详细研究了MBBG-21A活性炭和YWD12E大孔树脂对大豆粗糖浆的脱色效果。实验表明,活性炭脱色最佳条件为：活性炭添加量1．5％,pH4.0,脱色温度40℃,脱色时间60min;树脂脱色最佳条件为：pH4．0,脱色温度30℃,脱色时间60min。     

离子交换树脂对大豆糖蜜上清液脱盐脱色工艺的研究

将001×8强酸性阳离子交换树脂柱和D301-G大孔弱碱性阴离子交换树脂柱串联,通过动态吸附试验,对大豆糖蜜上清液进行脱盐脱色工艺条件的研究。结果表明,最佳脱盐脱色条件为:001×8强酸性阳离子交换树脂柱和D301-G大孔弱碱性阴离子交换树脂柱体积比为1∶2,流速为2.6 BV/h,大豆糖蜜上清液体积分数30%,pH 7,处理温度室温。在此条件下,处理2.0 BV大豆糖蜜上清液,脱盐率为94.89%,脱色率为88.70%,大豆低聚糖含量提高20.80%;001×8强酸性阳离子交换树脂采用1 mol/L的HCl解吸再生,D301-G大孔弱碱性阴离子交换树脂采用1 mol/L的NaOH解吸再生后,串联树脂柱可以重复使用9次。     

大豆乳清废水膜过程生产低聚糖工艺研究

主要探讨了乳清废水膜过程生产低聚糖等产品工艺中各级膜滤过程和其他流程试验,采用正交试验确定了低聚糖液的活性炭最佳脱色条件:脱色时间30 min,加炭量2.0%,脱色温度70℃,pH4.0,同时还对脱色后的低聚糖液的树脂脱盐试验进行了研究.乳清废水经过该工艺过程,最终形成了大豆低聚糖产品,超过了主产品的效益,水质接近纯净水并回用至工序中.     

大豆低聚糖精制工艺的研究

用乙醇溶剂法从大豆脱脂豆粕中提取大豆浓缩蛋白,以此大豆乳清液为原料,浓缩除醇后得到大豆低聚糖粗糖液,经过络合沉淀法除蛋白、离子交换树脂脱盐、活性炭脱色等一系列的纯化处理,浓缩后得到大豆低聚糖成品.系统介绍了大豆低聚糖生产工艺参数,使其能进入工业化规模生产.     

棉籽壳木聚糖提取液的脱色工艺研究

以伊犁产棉籽壳为原料,比较3种活性炭和3种阴离子交换树脂对棉籽壳木聚糖提取液的脱色效果,确定了最佳脱色条件.结果表明:LY-T-ac型活性炭和D392型阴离子交换树脂对棉籽壳木聚糖液有较好的脱色效果.D392型阴离子交换树脂最佳脱色条件为:脱色温度50℃、pH8.0、脱色时间180 min(150 r/min),在树脂添加量为75 g/L、糖液固形物含量为6％时,脱色率为93.66％.LY-T-ac型活性炭最佳脱色条件为:脱色温度80℃、pH5.0、脱色时间70 min(150 r/min),在活性炭添加量为3g/L、固形物含量为6％时,脱色率为93.51％.     

D-核糖离子交换提取工艺

采用离子交换法提取发酵液中的D-核糖,发酵液除蛋白、脱色后,树脂001x4与D301R组合脱盐,脱盐率为74.29%、D-核糖回收率为92.28%.从16种树脂中选择了D-380树脂作为提取D-核糖的树脂.在30℃,料液pH<4.0,流率lml/min下,D380树脂对D-核糖的交换容量为89.47mg/ml,pH10.00的NaOH溶液作洗脱剂,解析率为8.97%.     

从棉子糖肠球菌发酵液中提取γ-氨基丁酸的初步研究

针对实验室筛选到一株高产γ-氨基丁酸(γ-aminobutyric acid,GABA)的棉子糖肠球菌(Enterococcus raffinosus)TCCC11660菌株,研究了壳聚糖絮凝剂预处理该发酵液的工艺条件。以絮凝率为指标,确定了适于GABA发酵液体系的絮凝工艺。结果表明,最佳操作条件为在室温下发酵液pH4.5,壳聚糖用量150mg/L,150r/min快速搅拌加入絮凝剂后25~30r/min慢速养絮15min,絮凝率可达95.2%,GABA保留率为99%。絮凝后的发酵液采用大孔吸附树脂进行脱色处理,筛选出的DA201-CⅡ树脂具有较高的脱色率和GABA得率。实验确定了最佳脱色条件为:25℃,pH4.5,处理体积5BV,脱色流速3BV/h。采用此工艺对GABA发酵液的脱色率达到90.6%,GABA得率为92.5%。     

从豆清水中提取大豆低聚糖的工艺研究

介绍了从豆清水中提取大豆低聚糖的工业化生产工艺,并对成品大豆低聚糖的基本性质进行了研究.实验选择高温离心分离法脱除豆清水中的蛋白质,温度选择90℃,脱除率达85.12％.选择电渗析法进行脱盐处理,脱除率达85.8％.使用粉末活性炭进一步进行脱色,采用单因素及正交实验对脱色工艺进行研究,最佳脱色工艺为活性炭用量0.3％,40℃下脱色35min,脱色率达83.71％,低聚糖保留率为80.41％.     

低温蛋白酶MP发酵液离子交换树脂的脱色

低温蛋白酶MP发酵液中的色素沉积影响了其在产品中的应用,该文研究了利用离子交换树脂对其进行脱色。实验结果表明,阴离子树脂D352既能有效地脱色,又能保持较高的蛋白酶酶活;D352树脂脱色的最适pH值为6⁷,最适温度为30℃,最有效的流速为6 BV/mL;D352树脂在吸附色素的同时也能吸附发酵液中大量杂蛋白分子,蛋白脱除率为45.2%;D352树脂在连续使用6次之后仍具有较强的脱色能力。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press