共查询到18条相似文献,搜索用时 125 毫秒
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在分离分析的实践中,广大研究人员发展了多种样品富集方法以提高毛细管电泳技术的检测灵敏度。该文评述了国内外近年来在毛细管电泳技术的样品富集方法方面的最新进展,包括在线堆集、pH调制、动态pH联接、扫集、液液萃取、移动反应界面法等富集技术,并介绍了这些技术的应用实例。共引用文献41篇。 相似文献
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毛细管电泳及其在分离分析中的应用进展 总被引:4,自引:0,他引:4
毛细管电泳技术是一种重要的生物分离分析技术,讨论了该技术的作用原理,分析了影响毛细管电泳的因素,并且较详细地介绍了这一技术在生物分离分析领域的应用,还对其今后的发展前景进行了展望。 相似文献
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综述了毛细管电泳(CE)测试环境样品的基本原理、特点及在环境监测中的应用现状,并对其在环境监测领域的应用研究前景进行展望。 相似文献
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毛细管电泳化学发光检测在药物分析中的应用 总被引:1,自引:1,他引:0
药物检测和药物代谢研究在药理学和生物医学领域越来越受到重视。毛细管电泳化学发光技术,是当今国际分析化学前沿领域中一种极具潜力的微分离检测技术,它集成了电泳分离的高效、化学发光检测的高灵敏等特点,主要优势在于:费用低廉、简便快捷、样品用量少、污染小。本文对毛细管电泳化学发光法在药物分析中的应用予以综述。 相似文献
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黄酮类化合物在牙膏中的应用 总被引:1,自引:0,他引:1
黄酮类化合物具有抗茵、消炎、抗突变和清热解毒等多种生物活性,将其作为添加剂而研制的牙膏有止血、消炎、除口臭和愈伤等多种疗效。将黄酮类化合物中常见的黄芩苷加入牙膏,通过疗效检测,得出以下结论:在复方牙膏中添加0.05%的黄芩苷和0.02%的叶绿素后,疗效达到92%以上。 相似文献
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《分离科学与技术》2012,47(6):911-919
The aim of this research was to extend an existing capillary electrophoresis (CE) method, originally developed for the determination of mono- and disaccharides, to the determination of alternative carbohydrate compounds, namely furfural and polydatin. Empirical validation confirms that this novel method can be applied for the determination of analyte concentrations from complex matrices, and the evaluation of their carbohydrate composition. It is concluded that the approach has validity as an analytical procedure and has the ability to determine industrially important analytes from a heterogeneous biological sample matrix, and thus, the method adds value to the development of large scale separation processes. However, some additional optimization is required before online applications. 相似文献
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Petr Tma Blanka Sommerov Duan Koval Franois Couderc 《International journal of molecular sciences》2021,22(6)
Asymmetric and symmetric dimethylarginines are toxic non-coded amino acids. They are formed by post-translational modifications and play multifunctional roles in some human diseases. Their determination in human blood plasma is performed using capillary electrophoresis with contactless conductivity detection. The separations are performed in a capillary covered with covalently bonded PAMAPTAC polymer, which generates anionic electroosmotic flow and the separation takes place in the counter-current regime. The background electrolyte is a 750 mM aqueous solution of acetic acid with pH 2.45. The plasma samples for analysis are treated by the addition of acetonitrile and injected into the capillary in a large volume, reaching 94.5% of the total volume of the capillary, and subsequently subjected to electrophoretic stacking. The attained LODs are 16 nm for ADMA and 22 nM for SDMA. The electrophoretic resolution of both isomers has a value of 5.3. The developed method is sufficiently sensitive for the determination of plasmatic levels of ADMA and SDMA. The determination does not require derivatization and the individual steps in the electrophoretic stacking are fully automated. The determined plasmatic levels for healthy individuals vary in the range 0.36–0.62 µM for ADMA and 0.32–0.70 µM for SDMA. 相似文献