The effect of muscle, cooking method and final internal temperature on quality parameters of beef roast

The aim of the study was to evaluate the influence of cooking conditions (dry air and steam) and final internal temperature (75, 85, 95°C) on the physico-chemical properties of beef infraspinatus (INF) and semimembranosus (SEM) muscles as well as their tenderness and juiciness. Cooking method and temperature influenced moisture, total collagen content in cooked meat and cooking loss, whereas muscle type affected fat, total collagen content and cooking loss. Warner-Bratzler shear force values were affected by cooking method, which also influenced juiciness of roasts. Temperature affected tenderness and juiciness, whereas muscle type influenced juiciness. The most desirable tenderness had INF heated in steam and dry air to 95°C. Processing SEM in dry air to 85 and 95°C lowered the juiciness of the roasts. There were significant correlations between physico-chemical, sensorial and image attributes, however high accuracy of prediction (r(2)>0.8) was achieved only for SEM muscle.     

Evaluation of a probe for predicting beef tenderness

A series of three experiments were conducted to evaluate the performance of the MIRINZ (Meat Industry Research Institute of New Zealand) tenderness probe. In the first experiment, assessments on raw samples from two different muscles (longissimus lumborum and semimembranosus) were obtained using the tension head. Results indicated D50 values (torque at 50° of rotation) were the most highly related to both sensory (taste, olfactory, and feeling factors measured by a trained sensory panel) and consumer (hedonic ratings provided by untrained consumers) properties and were the values of choice, since all probe values were essentially measuring the same characteristics. In the second experiment, assessments were made using both the tension and shear heads on raw and cooked striploin (longissimus lumborum) steaks. Results confirmed probe values were essentially measuring the same characteristics, but indicated raw tension head, area 2 values (area before 50° of rotation) were more highly related to both sensory and consumer properties than were D50 values. However, probe values from raw samples did not account for a sufficient amount of variation in either sensory or consumer properties to be useful predictors of these traits, and stepwise, linear regression did not improve the predictive value of raw probe values, since all probe values were interrelated. Probe values on cooked samples indicated the shear head and area 3 values (area under the entire trace) were the most related to both sensory and consumer properties and were the values of choice. Based upon the amount of variation accounted for in sensory and consumer traits, probe area 3 values using the shear head on cooked samples provided greater predictive value for both sensory and consumer traits than Warner–Bratzler shear values obtained from steaks cooked either fresh or after being frozen and thawed. In experiment 3, correlations with individual, parametric character notes from the texture profiles of two different muscles provided no clear indication as to the textural properties being assessed by the probe. However, the MIRINZ probe appears to be a quick viable alternative to the Warner–Bratzler shear for providing an objective measure of cooked meat tenderness.     

Time-resolved reflectance spectroscopy for non-destructive assessment of food quality

In the majority of most food and feed, visible, and near infrared light undergoes multiple scattering events and the overall light distribution is determined more by scattering rather than absorption due to the microscopic spatial changes in the refractive index. Conventional steady state reflectance spectroscopy can provide information on light attenuation, which depends both on light absorption and light scattering, but cannot separate these two effects. In contrast, time-resolved reflectance spectroscopy (TRS) allows more detailed optical characterization of diffusive media in terms of their absorption coefficient and reduced scattering coefficient. From the assessment of the absorption and reduced scattering coefficients, information can then be derived on the composition and internal structure of the medium. The main advantages of the technique are the absolute non-invasiveness, the potentiality for non-contact measurements and the capacity to probe internal properties with no influence from the skin. In this work we review the physical and technical issues related to the use of TRS for non-destructive quality assessment of fruit and vegetable. A laboratory system for broadband TRS, based on tunable mode-locked lasers and fast micro-channel plate photomultiplier and a portable set-up for TRS measurements, based on pulsed diode lasers and compact metal-channel photomultiplier, are described. Results on broadband optical characterization of fruits and applications of TRS to the detection of internal defects in pears and to maturity assessment in nectarines are presented. Conference: SPIE OPTICS EAST, 9–12 September 2007, Seaport World Trade Center, Boston, MA USA; Session: Optics for Natural Resources, Agriculture, and Foods II; Conference Chairs: Yud-Ren Chen, USDA Agricultural Research Service; George E. Meyer, Univ. of Nebraska/Lincoln; Shu-I Tu, USDA Agricultural Research Service.     

The effects of method of castration,rearing condition and diet on sensory quality of pork assessed by a trained panel

Beside surgical castration possible alternatives helping to reduce the incidence of boar taint in cooked pork are rearing conditions, immunocastration and feeding strategies for entire males known to lower the skatole levels. The goal of this study was to assess the effects of these alternatives on the sensory acceptability of pork. In experiment 1, carcasses from barrows, entire males (EM) and entire males fed raw potato starch (EM+) 7 d before slaughter were selected based on the androstenone (≤ 2 µg/g) and skatole (≤ 0.32 µg/g) levels. In experiment 2, carcasses from barrows, immunocastrates (IC), entire males either group-penned (EMG) or individually penned (EMP) were selected based on the aforementioned criteria. Boar odour and boar flavour intensities of longissimus dorsi (LD) and neck chops were evaluated by trained panellists. Boar odour and flavour scores were higher (P < 0.01) in neck than LD chops. Although skatole tissue levels in barrows and EM+ were similar (P > 0.05), boar odour and flavour scores were greater (P < 0.05) in EM+ than barrows. In experiment 2, scores for boar odour and flavour were lower (P < 0.05) in pork from barrows and IC than EMP, with intermediate values for EMG. In conclusion, we observed a discrepancy between the known boar taint compounds androstenone and skatole and sensory acceptability, which indicates that other factors influenced the perception of boar taint. Thus, surgical castration with or without anesthesia or immunocastration are still the safest methods to avoid boar taint.     

Chemical, instrumental and sensory characteristics of cooked pork ham

Instrumental, chemical and sensory parameters of cooked pork ham were evaluated. Principal component analysis was carried out on the basis of the instrumental variables related to colour and texture. The four PCs account for almost 94% of the total variance in the data set. The PCA only separated 3 hams with a^* > 10. Hardness was correlated with non-collagen muscle protein (P  0.01), gumminess (P  0.01) and ash (P  0.05). Sensory evaluated tenderness showed positive significant correlation with L^* (P  0.01). The most important colour parameter seems to be a^*, which was negatively correlated with sensory evaluated parameter colour (P  0.01). The PCA performed on all parameters (sensory, chemical and textural) discriminated two groups of hams differing in non-collagen muscle protein content and hardness.     

Effect of slaughter weight and breed on instrumental and sensory meat quality of suckling kids

The effects of breed and slaughter weight on chemical composition, fatty acid groups, texture, and sensory characteristics of meat of 141 suckling male kids from 5 Spanish breeds were studied. There was a decrease in texture and lightness and hue angle with the increase of the slaughter weight. Fatty acid composition was correlated with the intramuscular fat content. All the breeds except MO had values of n-6/n-3 ratio below 4, which is the healthy limit recommended, and a low atherogenic index as well as a low intramuscular fat content. A multivariate analysis discriminated light kid, which had the most tender and juicy meat, from heavy kid which had more intense kid and milk odours. Blanca Andaluza and Pirenaica had most tender and juicy meat. The effect of slaughter weight on meat traits should be considered separately for each breed to find the most appropriate meat according to consumers preferences.     

Modelling of sensory and instrumental texture parameters in processed cheese by near infrared reflectance spectroscopy

This study investigated the application of near infrared (NIR) reflectance spectroscopy to the measurement of texture (sensory and instrumental) in experimental processed cheese samples. Spectra (750 to 2498 nm) of cheeses were recorded after 2 and 4 weeks storage at 4 degrees C. Trained assessors evaluated 9 sensory properties, a texture profile analyser (TPA) was used to record 5 instrumental parameters and cheese 'meltability' was measured by computer vision. Predictive models for sensory and instrumental texture parameters were developed using partial least squares regression on raw or pre-treated spectral data. Sensory attributes and instrumental texture measurements were modelled with sufficient accuracy to recommend the use of NIR reflectance spectroscopy for routine quality assessment of processed cheese.     

Effects of oxygen concentration on the sensory evaluation and quality indicators of beef muscle packed under modified atmosphere

Beef steaks are commonly displayed under high oxygen concentrations in modified atmosphere packs (MAP) in order to promote colour stability. Such conditions, however, may also cause quality deterioration through lipid oxidation and decreased tenderness. The objective of this study was to investigate the effects of oxygen concentration (0%, 10%, 20%, 50% and 80%) on the quality of MAP beef steaks (M. longissimus dorsi). Steaks were stored at 4°C for 15 days and tested for lipid and protein oxidation, heme iron, colour, oxymyoglobin concentration, tenderness and sensory acceptability (up to day 12) for the resulting cooked meat. Sensory panellists expressed a preference for steaks stored in packs containing 50% oxygen, despite detecting oxidised flavours under these conditions. This could be the result of adaptation to, or familiarity with, oxidised flavours by panellists.     

Correlations of sensory and instrumental meat tenderness values as affected by sampling techniques

A study was conducted to determine if and to what extent higher correlations between sensory meat tenderness scores and Warner-Bratzler shear measurements could be obtained by giving the sensory panelists cores rather than slices. Thirty biceps femoris roasts, from gilts representing two breeds and two lines, were cooked in preheated ovens at 160, 170 or 180°C to an internal temperature of 80°C. Roasts were held for 24 h at 4°C before sampling. Cores, both perpendicular and parallel to the direction of the muscle fibres, for the eight panelists and the instrument, as well as slices for the panelists, were sampled from each roast. Instrumental values were measured with an Instron fitted with a Warner-Bratzler shear device. Correlations were higher when cores of the same orientation were used for panelists and the Warner-Bratzler measurements. Parallel cores did as well as or better than perpendicular cores in terms of the analysis of variance and correlation values.     

Opportunities for predicting and manipulating beef quality

Meat quality is a complex concept and can be defined as the characteristics of meat which satisfy consumers and citizens. The quality concept can be divided into intrinsic quality traits (which are the characteristics of the product itself) and extrinsic quality traits (which are more or less associated to the product for instance the price, a major determinant of purchase, or any brand or quality label). Quality can also be generic for the mass market or specific for niche markets. The relative importance of the different quality traits varies with human culture and time with a general trend of an increasing contribution of healthiness, safety and extrinsic quality traits. This review underlines the need for the development of methods to interpret and aggregate measures under specific rules to be defined in order to produce an overall assessment of beef quality. Such methods can be inferred for example from genomic results or data related to muscle biochemistry to better predict tenderness or flavor. A more global assurance quality scheme (the Meat Standards Australia System) based on the aggregation of sensory quality traits has been developed in Australia to ensure palatability to consumers. We speculated that the combination of indices related to sensory and nutritional quality, social and environmental considerations (carbon footprint, animal welfare, biodiversity of pasture, rural development, etc.) and economic efficiency (incomes of farmers and of others players along the supply chain, etc.) will provide objective assessment of the overall quality of beef (i.e. incorporating an all encompassing approach) not only for the mass market but also to support official quality labels of niche markets which are so far mainly associated with the geographical origins of the products.     

Establishing tenderness thresholds of Venezuelan beef steaks using consumer and trained sensory panels

The tenderness thresholds for ribeye steaks at 2 d postmortem were found by using sensory trained (n = 734 steaks) and consumers panel data (55 ribeye steaks and 296 consumers). Also, consumers’ perceptions of sensory attributes of beef steaks were assessed to establish their relationships and to determine acceptability levels. The tenderness threshold obtained by regression analysis from trained panelists was 37.98 N (3.87 kg). Customer satisfaction of 81% was obtained when beef steaks had Warner-Bratzler shear force values (WBSF) less than 40.13 N (4.09 kg), suggesting that 40.13 N could be considered as a tenderness threshold to segregate tough from tender steaks, for Venezuelan beef consumers. According to correlation and regression analyses, consumers focused on flavor to assign their overall liking score. Results suggest that consumers are able to detect changes in tenderness of steaks with different WBSF and that flavor drives customer satisfaction when the beef tenderness is acceptable.     

The use of near infrared reflectance spectroscopy for predicting the quality of grass silage

Near infrared reflectance spectroscopy, using a fixed-filter instrument fitted with 19 filters, was evaluated for the prediction of crude protein and in-vitro dry matter digestibility in commercial farm grass silage. Crude protein (6.5–17.6%) was estimated with acceptable accuracy (standard error of prediction=0.63%) using a six-wavelength calibration, but in-vitro dry matter digestibility (40.7–69.9%) was not predicted with sufficient accuracy (standard error of prediction=2.96%) by this technique.     

Pelvic suspension and fast post-mortem chilling: Effects on technological and sensory quality of pork - A combined NMR and sensory study

The effects of pre-rigor excision, which results in a fast post-mortem chilling; pelvic suspension, which results in a muscular stretching; control treatment of m. longissimus dorsi on water distribution measured by (1)H NMR relaxometry and on sensory properties were investigated in two sub-studies including a total of 16 pigs. Determination of sarcomere length revealed significant effects of the treatments on the degree of contraction, as pre-rigor excision and pelvic suspension resulted in shorter and longer sarcomere length, respectively. In addition, an effect of treatment on pH measured at 24h post-mortem was found, as pre-rigor excision was associated with a higher ultimate pH. NMR measurements revealed that water distribution in the meat was affected to a minor degree by the various treatments. However, an interaction between treatment and ageing period was observed, as the data demonstrated an effect of pre-rigor excision on water distribution in the cooked samples when meat was only aged for 2 days, but this effect was eliminated when meat was aged for 5 days. The effect of pre-rigor excision on water distribution in cooked meat after 2 days of ageing is suggested to reflect structural constraints in the meat that are eliminated during ageing. Sensory analyses demonstrated strong interactions between treatment and position on the muscle and between treatment and ageing period. However, in general pre-rigor-excised meat was sensed as significantly juicier compared with the other two treatments. Accordingly, the study demonstrates that under the present conditions the ultimate pH is more important for juiciness than the sarcomere length.     

Evaluation of near-infrared reflectance spectroscopy for predicting stover quality trait in semi-exotic populations of maize

Near-infrared reflectance spectroscopy (NIRS) analysis was investigated as a means of predicting quality parameters in semi-exotic maize stover. These parameters included crude protein (CP), neutral detergent fibre, acid detergent fibre and in vitro dry matter digestibility (IVDMD). Samples of semi-exotic maize stover were collected during three growing seasons (1989, 1990, 1991) from three locations in Catalonia, Spain. Calibration equations were obtained by multiple linear regression of conventional laboratory values on NIRS data from 84 samples and verified with 20 additional samples. Separate NIRS calibration were developed also within year (1989 and 1990, respectively). A Bran + Luebbe InfraAnalyzer model 450 was used for the study. In the multi-year calibration the coefficients of squared multiple correlation (R²) ranged from 0–81 for IVDMD to 0–92 for CP and the standard errors of calibration (SEC) ranged from 0–35 for CP to 1–46 for IVDMD. The study showed that NIRS analysis can be used to evaluate the quality of semi-exotic maize in breeding programmes.     

The influence of ante-mortem treatment on relationship between pH and tenderness of beef

Longissimus lumborum et thoracis muscles from 150 cattle comprising Czech Pied breed bulls and cows were used to investigate the relationship between pH value and beef tenderness as influenced by housing before slaughter and by the hanging mode of carcasses. The pH value was measured in the loin at the end of the slaughter-line (pH(0)) and after cooling of carcasses (pH(24),pH(48)). The texture of raw meat was measured 72h post-mortem by the Warner-Bratzler shear method; the shear force was related to pH. Curvilinear relationships with coefficients of correlation r=0.64 (P<0.05) for bulls individually housed and r=0.83 for bulls housed in groups between the shear force and the pH value 24h p.m. were found with maximal shear force (the most tough meat) in the range of pH(24) from 6.2 to 6.4. Lower correlations (r=0.72 and r=0.54) between pH(24) and meat tenderness were found in the case of cows individually housed and housed in groups, respectively. No significant relationships were found between texture and pH measured at the end of the slaughter-line (r=0.59 for bulls and r=0.65 for cows) and 48h post-mortem (r=0.64 for bulls and r=0.50 for cows). The mode of housing of cattle before slaughter influenced the properties of beef significantly. Meat of bulls housed in groups before slaughter was less tender (the shear force was higher) than meat of bulls individually housed. It could be explained by higher depletion of glycogen before slaughter and thus insufficient pH decline. Generally, the meat of cows was more tender than of bulls irrespective of housing. No significant differences were found between the left and right halves of carcasses. Thus the mode of hanging had no influence on beef tenderness.     

National beef tenderness survey - 2006: Assessment of Warner-Bratzler shear and sensory panel ratings for beef from US retail and foodservice establishments

Beef from retail and foodservice establishments in 11 US cities was evaluated using Warner-Bratzler shear (WBS) and consumer evaluation panels. Postmortem aging times ranged from 3 to 83d for retail and 7 to 136d for foodservice with mean aging times of 22.6d and 30.1d, respectively. For retail, the three cuts from the round - top round, bottom round, and eye of round - had the highest (P<0.05) WBS values compared to cuts from the chuck, rib, and loin. Top loin steaks had the lowest (P<0.05) WBS value compared to ribeye and top sirloin foodservice steaks. Retail bone-in top loin, top loin, ribeye, T-bone, and porterhouse received the highest (P<0.05) ratings by consumers for overall like and like tenderness. Quality grade had little or no effect on foodservice sensory evaluations. Improvements in round tenderness are needed to increase consumer acceptability.     

Water distribution and mobility in meat during the conversion of muscle to meat and ageing and the impacts on fresh meat quality attributes--a review

This paper reviews current knowledge on the distribution and mobility of water in muscle (myowater) ante- and post mortem and factors affecting these in relation to fresh meat quality parameters; water-holding capacity (WHC), tenderness and juiciness. NMR transverse relaxometry (T(2)) using bench-top Low-Field Nuclear Magnetic Resonance (LF-NMR) has characterised myowater distribution and mobility as well as structural features in meat which directly affect WHC. The current literature demonstrates that WHC is correlated to the water located outside the myofibrillar network (extra-myofibrillar). This review identifies the critical stages which affect the translocation of water into the extra-myofibrillar space and thus the potential for decreased WHC during proteolysis (the conversion of muscle to meat). This review discusses how the intrinsic properties of the water held within the meat could contribute to juiciness and tenderness. Tenderness has been shown to correlate to T(2), however breed and species differences made it difficult to draw firm conclusions. Further understanding of the inherent water properties of fresh meat and the factors affecting water distribution and mobility using NMR technologies will increase the understanding of WHC and tenderisation of fresh meat.     

Processing and sensory characteristics of cooked roast beef: effect of breed, age, gender and storage conditions

Two separate experiments investigated the effect of breed (Bos indicus, Bos indicus×and Bos taurus), gender/age and chilled and frozen storage on the processing characteristics of roasts made from inside rounds (semimembranosus, adductor and gracilis muscles). Roasts were injected with a salt/phosphate brine and cooked to an internal temperature of 63 °C. Freezing the meat increased purge lost from the raw meat. Country of origin had no significant effect on cook yield. Sensory evaluations with a US consumer panel indicated only slight sensory differences due to country of origin and breed but a significant effect from storage regime. Tenderness comparisons between breeds were confounded by country of origin and indicated that roast beef from inside rounds from Brahman cattle had lower WBS values than roast beef made from inside rounds from British cattle. Although breed and age/gender often significantly affected processing characteristics of the roasts, differences were small and would have little practical or economic importance for selecting the type of meat for this market sector.