固定化金属离子亲和分配用的亚胺二乙酸-聚乙二醇偶联物的制备

The synthesis route was investigated and optimized for the preparation of iminodiacetic acid polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in aqueous two-phaze systems. IDA PEG was synthesized from PEG in two steps by the reaction of iminodiacetic acid with a monosubetituted derivative of epichlorohydrin-activated PEG. The Cu2+ content combined with IDA-PEG was determined by atomic absorp tion spectrometry as 0.5 mol.mol-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase in polyethylene glycol/hydroxypropyl starch aqueous two-phaze systems was studied to clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG.     

Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity Partitioning

1 INTRODUCTIONImmobilized metal ion affinity chromatography(IMAC) is a powerfu tool for the separation ofproteinsI1'2]. Metal amnity separation exPloits theaffinity of transition metal ions for electron-rich aminoacid residues, such as histidine and cysteine, which areaccessible on the surface of proteins. Metal recogni-tion can also be engineered into prOteins for aPplication such as protein purification[3]. Recelltly, immobi-     

非晶态合金催化剂用于二乙醇胺氧化合成亚氨基二乙酸

采用自制的非晶态合金催化剂进行二乙醇胺氧化合成了亚氨基二乙酸 ,探讨了催化剂用量、反应温度、反应时间、氢氧化钠用量、反应压力等因素对氧化反应的影响 ,确定了最佳工艺条件 ,并考察了催化剂的重复使用效果     

The Enzymatic Synthesis and Characterization of Disolketal Iminodiacetic Acid (DSIDA)

Disolketal iminodiacetic acid (DSIDA) has been synthesized from the enzyme‐catalyzed condensation reaction between derivatives of iminodiacetic acid (IDA) and glycerol. According to all available literature, DSIDA is a novel diester that has never been synthesized. It is a precursor to water‐soluble polyhydric alcohols and helps to address the global need of the biodiesel industry to find new uses for glycerol. Reacting diMe‐IDA with solketal, a protected glycerol, produced DSIDA in yields as high as 96.4% under optimal reaction conditions of 70 °C and 200 Torr for 24 h. The reaction was monitored using ATR‐IR and a validated GC method. ATR‐IR monitored the disappearance of the primary solketal alcohol and the appearance of cyclic solketal ether bonds in the molecular backbone of the intermediates and product. Structural analysis of the intermediates and products was performed using two‐dimensional NMR, GC, GC–MS and elemental analysis. All spectral data was in agreement with the proposed structures for the chemical reaction. Thermal profiles were determined by TGA to be single‐stage decompositions above 160 °C.     

葡聚糖接枝型高载量金属螯合介质的制备与性能

对琼脂糖凝胶微球进行烯丙基活化,再接枝葡聚糖分子,考察葡聚糖分子量等因素对葡聚糖接枝过程的影响;以葡聚糖接枝琼脂糖凝胶微球为基质,制备亚氨基二乙酸型金属螯合介质,考察葡聚糖接枝过程对金属螯合介质的孔道结构、流通性能和载量等的影响. 结果表明,分子量20~500 kDa的葡聚糖都能均匀分布于琼脂糖凝胶微球内,葡聚糖接枝量随分子量增加而增大,所制的金属螯合介质形貌、粒径及其分布基本不受影响,且具有更好的流通性能,孔道结构比商品介质Ni Sepharose 6FF更丰富. 葡聚糖接枝的金属螯合介质对带组氨酸标签的乳酸脱氢酶和睫状神经营养因子的载量分别达到19和27 mg/mL,较Ni Sepharose 6FF的载量分别提高26.6%和42.0%.     

亚氨基二乙酸的生产技术及其应用

介绍了亚氨基二乙酸的物理和化学性质,叙述了亚氨基二乙酸的不同合成方法及其在农药工业、染料工业、水处理工业等领域的应用.     

Synthesis of Oleic Acid Esters with Immobilized Lipase

Esterification of oleic acid and alcohols with immobilized lipase from fungus Mucor miehei in a stirred batch reactor is presented in this article. The degree of conversion of oleic acid to esters was determined by measurement of acid values. The dependence of the synthesis of n-butyl oleate on the amount of enzyme, the influence of temperature on the equilibrium conversion for this reaction and the influence of temperature on initial velocities is presented. The influence of water added to reaction mixture at various reaction conditions on the activity of the enzyme preparation is also presented.     

磺酸萜烯树脂的合成及对金属离子吸附研究

研究了磺酸萜烯树脂的合成方法。用红外光谱对合成树脂进行了表征 ,测试了合成树脂的离子交换容量 ,及对多种金属离子的吸附性能。结果显示合成树脂能与Co2 + 、Pb2 + 、Cu2 + 、Fe3 + 等金属离子络合 ,能用于金属离子的吸附分离     

环氧聚硅氧烷体系开发与应用

本文论述了环氧－聚硅氧烷改性体系的种类,制备方法,性能特下及应用,并介绍了笔者所在课题组在该体系中研制空的新结果。     

Cu~(2+)-IDA金属螯合尼龙-壳聚糖复合膜的制备及其亲和分离性能研究

为获得既具有良好机械强度和化学稳定性又具有大量活性官能团的金属螯合亲和膜介质,以尼龙膜为基膜,采用环氧氯丙烷活化共价偶联壳聚糖,制备尼龙-壳聚糖复合膜,膜上偶联壳聚糖的含量达98.2 mg·(g膜)-1,为配基的固载提供了大量活性位点.再次采用环氧氯丙烷活化复合膜,进而偶联亚氨二乙酸(IDA)、固定化Cu2+,获得金属螯合尼龙-壳聚糖复合型亲和膜,配基Cu2+固载量为5.4μmo1·cm-2.以牛血清白蛋白为目标蛋白,考察亲和膜的分离性能.研究结果表明:牛血清白蛋白在膜上的吸附行为符合Langmuir吸附等温方程,膜对牛血清白蛋白具有较好的亲和吸附效果,吸附容量达1.09 mg·cm-2.该膜具有较长的使用寿命并且容易再生.     

柠檬酸为模板剂制备氧化锌气凝胶

以氯化锌为原料,柠檬酸为模板剂制备了锌基复合气凝胶,通过场发射扫描电镜（FESEM）、高分辨透射电镜（HRTEM）、X射线衍射（XRD）、N2吸脱附以及红外谱图（FTIR）分析对气凝胶的结构进行了表征,结果表明：锌基复合气凝胶是由大量纳米级的球状颗粒堆积而成的,具有典型的三维网络结构,其骨架是由大量非晶态的碳层包覆的氧化锌胶体颗粒所组成的;气凝胶具有较高的比表面积为177m2/g,孔径分布主要集中在10-60nm左右,为介孔材料。     

金属螯合双水相亲和分配技术分离纳豆激酶的研究

利用金属螯合亲和双水相分配技术对纳豆激酶的分离纯化进行了研究。考察了双水相系统、聚合物的分子量和浓度、亲和配基加入量、pH值、相比以及生物质加入量等因素对亲和分配的影响。结果表明,双聚合物系统比聚合物／无机盐系统更有利于纳豆激酶亲和分配;pH值和亲和配基加入量是影响分配的关键因素。优化的分配条件为：2．6％聚乙二醇,20．2％羟丙基淀粉,5％亲和配基PEG-IDA—Cu(Ⅱ),相比12,pH8．2,发酵液加入量15％。分配系统放大到100g,仍保持一致的酶活收率(90％)和纯化因子(2.0)。设计了两次分配分离流程,纯化因子达到3．52,总收率为81％。     

锌基复合气凝胶的制备与表征

以氯化锌为原料,柠檬酸为模板剂制备了锌基复合气凝胶,通过场发射扫描电镜(FESEM)、高分辨透射电镜(HRTEM)、X射线衍射(XRD)、N2吸/脱附以及红外谱图(FTIR)对气凝胶的结构进行了表征,结果表明:锌基复合气凝胶是由大量纳米级的球状颗粒堆积而成,具有典型的三维网络结构,其骨架是由大量非晶态的颗粒组成的;气凝胶具有较高的比表面积为177 m2/g,其孔径分布主要集中在10~60 nm,为介孔材料。     

聚硅氧烷聚氨酯的合成与性能

氨基硅油、甲苯二异氰酸酯与相对分子质量为1000和2000左右的聚氧化丙烯二醇在无溶剂条件下制备了相应的两类氨基硅油改性聚氨酯。测试结果表明,采用无环境污染的该法制备的改性聚氨酯与文献报道的溶剂法制备的改性聚氨酯具有类似的改性效果,改性后的聚氨酯兼有有机硅和聚氨酯的特性。w(氨基硅油)=3%～15%时,有较明显的改性效果,且在w(氨基硅油)=10%时,两类改性聚氨酯都具有最佳综合性能,其伸长率较未改性的分别提高28 05%,52 38%,其表面水接触角分别提高了23°,25°,耐热性也有较大提高。     

聚氨酯改性有机硅的合成与性能

用含氧硅油、单烯丙基封端的聚醚和甲苯二异氰酸酯制备了聚氨酯改性有机硅乳液。并用高分辨电镜观察了乳液的粒径及其分布。研究了不同n（NCO/n（OH）值对乳液性能、成膜力学性能及热稳定性的影响.     

嵌段型聚醚有机硅的合成及应用研究

在氯铂酸催化剂的作用下,以含氢硅油与羟基封端的烯丙基聚醚为原料,进行硅氢化加成反应,合成出了一种嵌段型聚醚聚硅氧烷（PESO）。探究了反应条件对合成PESO的影响,确定了合成PESO的最佳反应条件：温度为85～90℃,催化剂用量为20ppm,反应时间为4h。用IR谱、1H—NMR谱对PESO进行了结构表征。再将产品PESO在布样上应用,性能测试表明其吸水性、柔软性提高了,而抗皱防缩性降低。在此基础上,又通过扫描电镜（SEM）、原子力显微镜（AFM）对PESO在纤维和单晶硅基质表面的膜形貌进行了讽察和研奔．     

金属络合染料酸性黑ACE的合成

2-氨基-4-硝基苯酚重氮化后,分别在酸性和碱性条件下与N-苯基-2-氨基-5-萘酚-7-磺酸偶合,得到偶氮物A和偶氮物B;6-硝基-1,2,4-酸氧体与2-萘酚偶合得到偶氮物C;偶氮物C和氧化铬络合反应得到络合物D;络合物D再与偶氮物A、偶氮物B在碱性条件下反应得到黑色非对称1∶2金属络合染料（酸性黑ACE）。本文介绍了该染料的化学结构及合成方法。     

Synthesis of Condensed Tannin Derivatives and their Protein-Precipitating Capacity

Four regiospecifically methylated condensed tannin derivatives were synthesized by the condensation of regiospecifically methylated flavan-3, 4-diols protected by benzyl groups, and subsequent debenzylation. The monomeric flavan-3,4-diols were obtained via four reaction steps starting from phloroacetophenone derivatives and protocatechualdehyde derivatives. Protein—precipitating capacity of these synthetic condensed tannin derivatives was tested and a comparision of these capacity suggests the following results: 1) Phenolic hydroxyl groups in tannins are essential sites for protein precipitation. 2) Although the A-ring has been considered not to be important for tannin—protein interactions, it was proved that the A-ring also plays an important role together with the B-ring. 3) Both hydroxyl groups of A- and B-rings may synergistically interact with proteins.     

以荷花花粉为模板合成分级多孔金属氧化物中空微球

以荷花花粉为模板,利用简便、经济的湿化学法合成系列分级多孔金属氧化物中空微球(MnO2、MnO3、La-CoO3),通过XRD、XPS、SEM、N2吸附-脱附分析对合成样品的物相结构与微观形貌进行表征.结果表明,荷花花粉模板法合成的MnO2、MnO3、LaCoO3中空微球成功复制了花粉的分级多孔结构,其比表面积分别高达...