The fertiliser nitrogen requirement of cereals grown on sandy soils

The responses to fertiliser‐N of winter wheat and winter barley grown on sandy soils were measured in 72 experiments in England from 1990 to 1994. Yield without fertiliser‐N (Y₀) was c 1.1 t ha⁻¹ greater following root crops than following cereals. Following potato crops given organic manures, Y₀ was c 1.2 t ha⁻¹ greater than following unmanured potato crops, but Y₀ was no greater following sugarbeet to which organic manures had been applied. Only after the two driest winters was there sufficient variation in soil N supply in spring (SNS_s) for this to show a relationship with Y₀. However, Y₀ increased with increasing N mineralisation during the growing season (AM) in the three years it was measured. There was no consistent effect of sowing date on Y₀. Following potatoes, yield at optimum fertiliser‐N (Y_opt) decreased as sowing date was delayed, but this was not so after cereals, sugarbeet or overall. There was no increase in Y_opt with SNS_S or AM, but Y_opt decreased with increasing moisture stress (S) in June. The mean yield response to N_opt (Δ_Y) was c 0.4 and 0.8 t ha⁻¹ smaller following potatoes and sugarbeet respectively than following cereals, but not consistently so as there were large interactions between site, year and previous crop. Following root crops, Δ_Y was c 0.6 and 1.4 t ha⁻¹ less after sugarbeet and potatoes respectively that had been given organic manures. Without the addition of organic manures, Δ_Y following potatoes was similar to that following cereals. Regression on SNS_S and AM accounted for 28 and 15% respectively of the variance in Δ_Y. The optimum economic fertiliser‐N application (N_opt) was similar, at c 140 kg ha⁻¹, following cereals and potatoes. Following sugarbeet, cereal N_opt was only c 110 kg ha⁻¹. The differences according to previous crop reported here are consistent with mineralisation of crop residues on sandy soils being more rapid than on other soils; the potato residues were rapidly mineralised in autumn and lost by leaching over winter. Residues from later‐harvested sugarbeet were mineralised during the growing season of the subsequent cereal crop. Fertiliser‐N requirements were, at c 110–140 kg ha⁻¹, smaller than has been found on other soil types, and less than current recommendations for wheat. Requirements were significantly reduced in years of drought stress. No differences were found in N_opt between wheat and barley. These data do not justify the current advice to invariably reduce fertiliser‐N to cereals following potatoes by 20–25 kg ha⁻¹ on these sandy soils. On average a reduction of c 20 kg ha⁻¹ could be made following sugarbeet, with a further reduction of c 40 kg ha⁻¹ N if manures had been applied to the previous sugarbeet crop. A reduction of 40 kg ha⁻¹ N could also be made where cereals followed a potato crop to which manures had been applied. Further refinements on the basis of measurements of soil mineral N could not be justified. Seasonal variation in N response due to drought stress makes recommendations difficult on these soils. Adopting the fertiliser‐N recommendations proposed here would produce N surpluses to the soil of c 37, 10 and 27 kg ha⁻¹ respectively following cereals, sugarbeet and potatoes when cereal grain is removed but straw incorporated. On farms where straw is removed, N surplus would be largely eliminated. Our recommendation that no reduction in fertiliser‐N application to cereal crops grown on sandy soils should be made following potatoes will not increase fertiliser‐N use and is not expected to increase nitrate leaching. Some reduction in nitrate leaching may be achieved if recommendations following cereal crops and sugarbeet are made in accordance with the results reported here. © 2000 Society of Chemical Industry     

Effects of perennial ryegrass cultivars on milk yield and nitrogen utilization in grazing dairy cows

The effects of 4 diploid perennial ryegrass cultivars that differed in water-soluble carbohydrate (WSC) concentrations on milk yield and nitrogen (N) utilization in dairy cows were evaluated in a 2-yr grazing experiment. Twelve lactating dairy cows were assigned to 1 cultivar for a 2-wk period in a 4 × 4 Latin square design with 3 replicates. Each year, the experiment lasted 8 wk. Swards were in a vegetative stage throughout the experiment. Herbage constituents were determined, and DM intake was estimated with the n-alkane technique. Nitrogen utilization was calculated as N excreted in milk divided by N intake, assuming a zero N retention. Two cultivars had consistently higher WSC concentrations and slightly lower neutral detergent fiber concentrations than the other 2 cultivars. The ranking of the cultivars in chemical composition traits in both years was rather consistent. Cows grazing the cultivar with the lowest concentration of WSC had the lowest herbage DM intake, N intake, milk yield, and milk N yield in 2002, but with a similar difference in WSC concentration, no differences among cultivars were found in 2003. In both years, milk urea N concentration was slightly higher for cows grazing the cultivar with the lowest WSC concentration, although it was significant only in 2003. Nitrogen utilization (N milk:N intake, g/g) varied between 0.241 and 0.246 in 2002 and between 0.190 and 0.209 in 2003, and in both years there was no effect of cultivar. At relatively high N concentrations in grass and only small differences among cultivars in neutral detergent fiber concentrations, cultivars with an elevated WSC concentration did not increase N utilization in grazing dairy cows.     

The contribution of fertiliser nitrogen to leachable nitrogen in the UK: A review

Evidence relating to nitrate leaching was taken from series of extensive field experiments conducted to support guidance on fertiliser use. Over the last 50 years, it is estimated that increased fertiliser N use on intensive wheat in the UK, has resulted in an increase of 36 kg N ha^?1 year^?1 leachable nitrate. Probably more than one-third of this change is due to larger yields resulting in a gradual build up in soil organic matter, the remainder to annual effects of fertiliser application. This justifies the association generally made between fertiliser used and nitrate leached and supports the value of some control of fertiliser use in order to restrict nitrate concentrations in drinking water.     

Dairy soil bacterial responses to nitrogen application in simulated Italian ryegrass and white clover pasture

Through clearing and use of fertilizer and legumes, areas of southwestern Australia's unique coastal sand plains can support relatively low-cost dairies. However, the ancient, highly weathered nature of the soils in this region makes the dairies susceptible to a range of threats, including nutrient leaching and erosion. Despite this, Western Australian dairy cows typically produce up to 5,500 L of milk per head annually supported by inorganic nitrogen (N) fertilizer (commonly 50:50 urea and ammonium sulfate) at rates up to <320 kg of N/ha per year. Where hotspots exist (up to 2,000 kg of N/ha per year), total N exceeds pasture requirements. We investigated plant and soil bacteria responses to N fertilizer rates consistent with Australian legislated production practices on dairy farms for pure and mixed swards of white clover (Trifolium repens) and Italian ryegrass (Lolium multiflorum) in a long-term pasture experiment in controlled glasshouse conditions. Although the soil bacterial community structure at phylum level was similar for white clover and Italian ryegrass, relative abundances of specific subgroups of bacteria differed among plant species according to the N fertilizer regimen. Marked increases in relative abundance of some bacterial phyla and subphyla indicated potential inhibition of N cycling, especially for N hotspots in soil. Ammonium concentration in soil was less correlated with dominance of some N-cycling bacterial phyla than was nitrate concentration. Changes in bacterial community structure related to altered nutrient cycling highlight the potential for considering this area of research in policy assessment frameworks related to nutrient loads in dairy soils, especially for N.     

The influence of two soil nitrogen levels on the utilisation of 15N-labelled ammonium nitrate by ryegrass

In a field plot experiment with grass under cutting management, two soil organic nitrogen levels (0.92% and 1.14% in the top 75mm of soil) were created by repeated applications of pig slurry over a period of 8 years. The influence of soil organic nitrogen level on the recovery by ryegrass of ammonium nitrate fertiliser was then studied by reseeding the plots and applying ¹⁵N-labelled fertiliser at four rates (40, 80, 120, 160kg N ha^?1). After each of the first four cuts unlabelled ammonium nitrate fertiliser was applied at these same rates. The percentage utilisation of the labelled fertiliser was measured in five harvests over 2 years. At the first cut the percentage utilisation averaged 46.4% and was independent of fertiliser rate and soil organic nitrogen level. The average percentage utilisation values in cuts 2, 3, 4 and 5 were 9.9, 2.4, 0.8 and 0.5 respectively. For the total of all cuts it was only at the 40 kg N ha^?1 fertiliser rate that the percentage utilisation was significantly different (P<0.05) between the 0.92% and 1.14% soil organic nitrogen levels, at 49.0% and 61.4% respectively. The soil nitrogen contribution to ryegrass at the first cut was significantly increased (P<0.05) by the high soil organic nitrogen level at the 40 and 160 kg N ha^?1 fertiliser rates. Over all fertiliser rates the average soil nitrogen contribution to the first cut was 50.4 and 61.1 kg N ha^?1 at the 0.92 and 1.14% soil organic nitrogen levels respectively. From the first cut data, soil organic nitrogen was estimated to have a net mineralisation rate of 2.6% year^?1 and a half-life of 26 years.     

Interaction between cow slurry and 15N-labelled calcium nitrate as fertilisers for ryegrass production

A field-plot experiment with ryegrass was carried out to study the interaction between ¹⁵N-labelled calcium nitrate at 0, 25 and 50 kg N ha^?1 and cow slurry supplying 0, 33 and 68 kg NH-N ha^?1. Treatments were applied on a single occasion to a site where first-cut silage had just been removed. The effects of the treatments on dry matter yield, nitrogen offtake and % utilisation of calcium nitrate were measured in two cuts of herbage taken after 5 and 17 weeks. Increases in dry matter yield due to slurry were smaller at the 50 kg N ha^?1 rate of calcium nitrate than at the 0 and 25 kg N ha^?1 rates, possibly due to non-linearity of the yield response curve. Nitrogen offtake data indicated that the two sources of nitrogen were taken up additively by the ryegrass. Rate of calcium nitrate application or slurry treatment had no significant effect on the % utilisation of calcium nitrate in either cut. The average values for % utilisation of calcium nitrate over all treatments were 50 and 7% at cuts 1 and 2, respectively. There was no evidence for enhanced rates of denitrification in the presence of slurry. Recovery values of nitrogen (estimated by difference) were lower for NH-N in slurry than for calcium nitrate. Volatilisation of ammonia from surface-spread slurry is the most likely reason for the low efficiency of NH-N in slurry.     

Zinc,copper and nickel concentrations in ryegrass grown on sewage sludge-contaminated soils of different pH

Sewage sludges containing high concentrations of zinc, copper and nickel were added separately to samples of two soils, a silty clay loam and a sandy loam, on which pH levels between 4.5 and 7.5 had been established; there were also treatments with sludge of low metal content or no-sludge. Soil-sludge mixtures were either continuously cropped with ryegrass or kept uncropped in pots in the glasshouse for 6 months. Zinc and nickel concentrations in 0.1 μM calcium chloride extracts of soils from the cropped pots and in solutions displaced from the fallow pots decreased with increasing pH over the range tested, but copper concentrations remained steady above pH 5.5; individual metal concentrations in ryegrass tops followed the same pattern with pH as those in extracted solutions. Squared correlation coefficients (R⁷) between shoot metal concentrations and concentrations of metals in EDTA, DPTA or calcium chloride extracts or displaced solutions, when taken over all soil, pH and sludge treatments, were >0.60 (P<0.001). Ryegrass yield reductions occurred on soils contaminated with each of the three metal sludges when soil pH was 5.5 or below.     

Effects of sodium fertiliser on the distribution of trace elements,toxic metals and water-soluble carbohydrates in grass and clover fractions

The distribution of trace elements, toxic metals and water-soluble carbohydrates was examined in the leaves, stems and inflorescences of perennial ryegrass and white clover, with and without sodium fertiliser. Copper and manganese were observed in both species to concentrate in the leaves and stems and iron and molybdenum in the leaves. In clover, nickel concentrated in the inflorescence. Differences in mineral concentrations between fractions were related to the mobility of the metal and its function in the plant. Sodium fertiliser approximately doubled the cadmium content of the leaves, stems and inflorescences of both species, reflecting cadmium mobilisation from the soil. The sodium fertiliser also reduced the zinc, copper, manganese and iron contents of grass but not clover and increased the molybdenum content of clover but not grass. Water-soluble carbohydrates were increased by sodium in grass but decreased in clover. Differences between the two species appeared to relate to nitrogen fixation by clover but not grass. © 1999 Society of Chemical Industry     

Effects of perennial ryegrass cultivars on intake, digestibility, and milk yield in dairy cows

The effects of 8 diploid perennial ryegrass (Lolium perenne L.) cultivars on dry matter (DM) intake, DM digestibility, and milk yield (MY) of dairy cows were evaluated in the summer of 2000 and 2001. Each summer, herbage was harvested daily and stall-fed to 12 dairy cows during six 2-wk periods. Six cultivars were fed in 3 periods (1, 3, and 5) according to a double 3 × 3 Latin square design. In the other periods (2, 4, and 6), 2 cultivars were fed in a repeated measurement design. Herbage mass and leaf blades in the sward canopy varied among cultivars, but differences were not consistent between years. The largest differences in herbage composition were found in water-soluble carbohydrate content, followed by crude protein content. only small differences were found in the neutral detergent fiber (NDF) content. A higher water-soluble carbohydrate content was found in 2 cultivars in both years, whereas ranking of cultivars in crude protein and NDF content was not consistent with years. Dry matter intake and MY were not affected by cultivar. In both years, DM digestiblity was high (>77%), with very small differences among cultivars in 2000 (<0.5%) and larger differences in 2001 (up to 4%). This was associated with a delayed heading date in 2001, resulting in larger differences in leaf blades and NDF content among cultivars. It may be concluded that the 8 cultivars used in our experiments do not provide grass breeders with encouraging evidence to include selection criteria for an increased DM intake, DM digestibility, and MY in their grass breeding schemes.     

The effects of mild water stress and soil type on the responses of grass and clover to sodium fertilizer

The effect of mild water stress on the response of herbage grown in sandy or loam soils to sodium nitrate fertilizer has been investigated. Sodium nitrate was applied at the equivalent of 32 kg Na ha⁻¹ to perennial ryegrass and white clover grown in pots with sand, loam and mixed soils and irrigated daily. One half of the pots were subjected to water stress by withholding irrigation water for three days each week. Water stress reduced herbage yield but did not affect the response to sodium fertilizer, except that the ash concentration of grass was increased only when water stress was applied as well as sodium fertilizer. The sodium fertilizer increased herbage sodium concentrations and decreased calcium and magnesium concentrations. It also increased the potassium concentration in clover and in herbage grown in sandy but not loam or mixed soils. Clover and grass had similar sodium and magnesium concentration, but clover contained more nitrogen, potassium and calcium than grass. Therefore, under the conditions of this experiment, the increase in herbage sodium concentration with sodium fertilizer was consistent and not affected by water stress, soil type or herbage species. Changes in herbage potassium concentration, however, were inconsistent, which detracts from the value of sodium fertilizer for reducing hypomagnesaemia in ruminants. © 1999 Society of Chemical Industry     

Effect of fertiliser on functional properties of flour from four rice varieties grown in Sri Lanka

BACKGROUND: Fertiliser is an essential agro‐chemical input in modern rice farming. Fertiliser affects the grain quality and yield of rice. Although much research has been carried out to investigate the influence of fertiliser (recommended NPK addition) on yield and quality of rice grain, little is known about the effect of fertiliser on thermal, pasting, gelling and retrogradation properties of rice flour. The aim of this study was to investigate the influence of recommended fertilisation on functional properties of rice flour from four popular high yielding rice varieties grown in Sri Lanka. RESULTS: Fertiliser (recommended NPK addition) increased the protein content but reduced the apparent amylose content in rice flour except in BG 357. Swelling power and amylose leaching of rice flour were also increased. Pasting onset temperature, cold paste viscosity and setback were increased but peak viscosity and granular breakdown decreased. In response to the fertiliser application, gelatinisation peak temperature was reduced in all varieties except BG 300. However, compared to pasting properties, gelatinisation parameters were not much affected by fertilisation. The extent of amylopectin retrogradation was decreased by fertiliser in BG 305 and BG 352 but unchanged in the other two varieties. Except in BG 305, fertiliser reduced the gel hardness of rice flour but increased the gel cohesiveness. CONCLUSION: It is apparent that the increased protein and reduced amylose content caused by fertiliser affect the functional properties of rice flour. Copyright © 2011 Society of Chemical Industry     

Preparation and composition of mesophyll,epidermis and fibre cell walls from leaves of perennial ryegrass (lolium perenne) and italian ryegrass (lolium multiflorum)

Cells of mesophyll, epidermis and residual fibrous material were obtained from leaves of Italian and perennial ryegrass harvested at different stages of maturity by mechanical disruption of leaf tissue. Mesophyll cells were selectively removed by filtration through 0.045 mm nylon mesh and remaining non-mesophyll cells centrifuged in metrizamide solutions (56–58% wt to vol.) of known density (1.308–1.329 g cm³ at 5°C) to obtain a pure epidermis cell fraction and a residual fibre fraction. Whole mesophyll cells contributed 63–72%, epidermis 12–15% and the fibre fraction 15–24% to the total leaf dry matter. Fibre values were higher in late-cut samples. Cell walls were prepared from mesophyll and epidermis cells by disruption and washing to remove cell contents. Fibre cells were judged free of cell contents and received no further treatment. Examination of cell wall preparations by light and electron microscopy showed that both mesophyll and epidermis preparations were essentially free from contaminating material. Mesophyll cell walls were uniformly thin (200 nm) while those of epidermis ranged from 2000–3000 nm at the outer face, thinning to 300 nm or less at the inner surface. An electron-light layer (cuticle) of approximately 200 nm thickness was present covering the outer face of the epidermis. The fibre fraction largely consisted of sclerenchyma, but contained, in addition, other vascular cells, detached annular rings and heavily silicified leaf hairs. Analysis of cell walls accounted for 85–90% of dry matter. Cellulose was the major component of all cell walls examined (approximately 40% of dry matter) with xylose residues accounting for a further 11% of mesophyll, 13.5–17.5% of epidermis and 21–25% of fibre cell walls. Arabinose was low in fibre cells but was present in much higher proportions in mesophyll and epidermis walls. The ratio of arabinose to xylose was approximately 1:1.5 for mesophyll, 1:2.5 for epidermis and 1:7.0 for the fibre fraction. The molar ratio acetyl to xylose remained fairly constant at 1:4 regardless of the grass, cell type or maturity of the sample. The uronic acid content of epidermis was higher than that in other cell types and showed an increase with increasing maturity of the grass, reaching over 9% in late-cut samples. Total phenolic material represented 2–3% of mesophyll and epidermis cell walls and 6% of fibre walls. Ferulic acid alone was released from the primary cell walls by saponification and p-coumaric and ferulic acids from the secondary-thickened fibre walls. Crude protein values (NX6.25) were high in mesophyll cell wall preparations and low in epidermis and fibre cell walls. Amino acid patterns were similar for both grasses and cell types but hydroxyproline was found in greater amounts in fibre cell walls than in either epidermis or mesophyll.     

Effects of grazing and nitrogen fertiliser on the soil microbial biomass under permanent pasture

Carbon and nitrogen in the soil microbial biomass were measured on eight occasions during a grassland production season, under swards of perennial ryegrass receiving 210 or 420 kg N ha^?1 per year or of ryegrass/white clover receiving no fertiliser N. The swards were either cut or grazed at monthly intervals. Soil microbial biomass C increased under all treatments in late April before declining until late July and rising slowly towards the end of the season. Values for microbial C under cutting and grazing were not significantly different but were usually larger under grass/clover than under grass. In contrast, the smallest values for soil microbial biomass N were recorded in late April. Larger N values were again recorded under grass/clover, and although values were nearly always smaller under grazing than under the corresponding cut treatment the differences were not significant. Soil biomass N comprised a large reservoir of mobile N, and at any one time contained 11, 3 and 5 times more N than was present in the mineral N component of the soil, the standing crop or excretal returns, respectively. It did not appear to be responsive, within a season, to the considerable returns of C and N from grazing animals or to N fertiliser management.     

Unused fertiliser nitrogen in arable soils—its contribution to nitrate leaching

Nitrate present in arable soils in autumn is at risk to leaching during the following winter. To see whether unused nitrogen fertiliser was a major source of this nitrate, ¹⁵N-labelled fertiliser was applied to 11 winter wheat crops at rates of between 47 and 234 kg N ha^?1in spring. The experiments were on three contrasting soil types in south-east England. On average, 17′% of the N from spring-applied labelled fertiliser remained in the 0–23 cm soil layer at harvest (range, 7–36%) but only a small proportion was in inorganic forms (ammonium + nitrate). This was never more than 5 kg N ha^?1and averaged only 1·3% of the fertiliser N applied (range, 0·4–3·6 %). Between 79 and 98% of the inorganic N in soils at harvest was unlabelled, being derived from the mineralisation of organic N rather than from unused fertiliser. The amount of unlabelled N was much greater where wheat was grown after ploughing up grass or grass/clover leys than where it was grown in all-arable rotations. When wheat was grown without N fertiliser, soil inorganic N content at harvest was no lower than in plots given fertiliser at rates up to 234 kg N ha^?1. This work indicates that, for soil growing winter wheat, almost all of the nitrate at risk to leaching over the winter period comes from mineralisation of organic N, not from unused fertiliser applied in spring. Consequently, even a drastic reduction in N fertiliser use would have little effect on nitrate leaching.     

Evaluating the use of plant hormones and biostimulators in forage pastures to enhance shoot dry biomass production by perennial ryegrass (Lolium perenne L.)

Fertilisation of established perennial ryegrass forage pastures with nitrogen (N)‐based fertilisers is currently the most common practice used on farms to increase pasture forage biomass yield. However, over‐fertilisation can lead to undesired environmental impacts, including nitrate leaching into waterways and increased gaseous emissions of ammonia and nitrous oxide to the atmosphere. Additionally, there is growing interest from pastoral farmers to adopt methods for increasing pasture dry matter yield which use ‘natural’, environmentally safe plant growth stimulators, together with N‐based fertilisers. Such plant growth stimulators include plant hormones and plant growth promotive microorganisms such as bacteria and fungi (‘biostimulators’, which may produce plant growth‐inducing hormones), as well as extracts of seaweed (marine algae). This review presents examples and discusses current uses of plant hormones and biostimulators, applied alone or together with N‐based fertilisers, to enhance shoot dry matter yield of forage pasture species, with an emphasis on perennial ryegrass. © 2015 Society of Chemical Industry     

Quality response of clonal black tea to nitrogen fertiliser,plucking interval and plucking standard

Variations in the black tea quality of high‐yielding clone S15/10 in response to rates of NPKS 25:5:5:5 fertiliser of 200 and 400 kg N ha⁻¹ year⁻¹, plucking intervals of 7, 14 and 21 days and a selective plucking standard of up to two leaves and a bud or an unselective plucking standard were studied. Generally, quality declined with longer plucking intervals and unselective plucking. Although there was a general decline in quality with increasing nitrogen rate, only the black tea total colour declined significantly on increasing the nitrogen rate from 200 to 400 kg N ha⁻¹ year⁻¹. For each nitrogen rate and each plucking interval, unselective plucking reduced the black tea quality. No significant interactions between any two of the three (nitrogen rate, plucking interval and plucking standard) or all three factors were noted, indicating that the patterns of response were similar. The results demonstrate that black tea quality changes due to the factors studied occur in the same pattern with variations in treatments. Poor black tea quality due to any of the factors studied cannot therefore be corrected by varying the other factors. © 2000 Society of Chemical Industry     

Prediction of the fertiliser needs of sugar-beet grown on fen peat soils

The fertiliser requirements of sugar-beet grown on fen soils were tested in 52 experiments from 1963 to 1969. In some fields, N.P.K and Na and, especially N and P, greatly increased yields, but the average responses obtained with the four elements were small. To help to predict where and how much fertiliser was needed for sugar-beet, soils were grouped according to soil analysis and classified by loss on ignition. Sugar-beet grown on soils with less than 20 μg/ml P (extracted with sodium bicarbonate) needed 1·20 cwt/acre P₂O₅, with 21–45 μg/ml P, 0·75 cwt/acre P₂O₅, and with more than 45 μg/ml P no fertiliser was required. Most soils contained high concentrations of K; with less than 100 μg/ml K (extracted with ammonium nitrate), 2·00 cwt/acre P₂O was probably justified; with 100–250 μg/ml, 1·00 cwt/acre K₂O was needed. Most of the responses to agricultural salt were by crops on soils with exchangeable sodium concentrations of less than 30 μg/ml Na. Loss on ignition was related to response to N fertiliser; when the loss was 14–25%, N increased yield by 6 cwt/acre sugar and 1·2 cwt/acre N fertiliser was Heeded and when the loss was 26–35%, the yield was increased by 3·5 cwt/acre and 0·6 cwt/acre N was needed. With greater losses there was little response.     

Effects of long-term herbicide use,irrigation and nitrogen fertiliser on soil fertility in an apple orchard

In 1972 an experiment was set up to investigate the long-term effects of herbicide, irrigation and two rates of nitrochalk fertiliser application on soil fertility in a Cox's Orange Pippin apple orchard. Samples taken in 1986 showed that uncultivated soil which had been maintained bare by herbicide had much lower organic C, total N and extractable K and Mg concentrations than soil which had been maintained under grass. Extractable P concentrations were lower in soil under grass than in soil under herbicide. In the absence of grass, soil pH was slightly lower than in its presence. All these effects were much greater at depths above 7·5 cm than below. Irrigation of the grass slightly increased organic C and total N levels at 0–7·5 cm compared with unirrigated grass but had no effect on extractable P, K and Mg. Increasing the fertiliser rate from 63 to 189 kg N ha^?1 had no effect on organic C, total N, extractable P and K. Yet, throughout the soil profile, extractable Mg concentrations were greater at the low than at the high N fertiliser rate. In a seedling growth test on soil taken from the orchard in 1988 (and confirmed to be free from residual herbicide), apple seedlings grown in soil which had previously been under grass grew significantly better than those in soil which had been bare. These differences were ascribed to a greater rate of N mineralisation in the soil formerly under grass. The results of this trial indicate that to safeguard soil fertility it is necessary to maintain a grass cover in the orchard. In addition, fertiliser application on newly planted trees should be adjusted to take account of the presence or absence of grass in the previous soil management treatment.     

Analysis of the temperature effect on the components of plant digestibility in two populations of perennial ryegrass

For the development of mechanistic models of herbage digestibility, quantitative insight into the effects of age, temperature and cultivar on digestibility characteristics of individual plant parts is needed. Towards that goal, glasshouse experiments were conducted at day/night temperatures of 13/8, 18/13 and 23/18 °C with vegetative and reproductive crops of two populations of perennial ryegrass (Lolium perenne L) selected for differences in leaf blade digestibility. Cell wall content (CWC) and true cell wall and organic matter digestibility (CWD and OMD) of vegetative and reproductive tillers were related to dimensions, mass, CWC and digestibility of separate plant parts. Compared with the vegetative tillers, the reproductive tillers had higher rates of leaf appearance, organic matter growth and CWD decline. Strikingly, for both tiller types, no direct effect of temperature on whole tiller CWD was observed, since temperature effects could be eliminated completely by relating CWD to development stage (DVS) expressed as number of leaves appearing on the main tiller. Temperature effects on CWD were restricted to its influence on tiller development rate only. The decline of CWD of individual plant parts with DVS in the reproductive tillers could be described with a negative exponential curve, which reached an asymptote that was higher for leaf blades (755 g kg^?1) than for leaf sheaths (491 g kg^?1) and stem internodes (230 g kg^?1). However, all plant parts in both tiller types had the same fractional CWD decline rate of 0.395 per leaf appearance interval, independent of plant part insertion level, population or temperature. Differences between temperature treatments in OMD were caused by the higher CWC of plant parts at higher temperature, due to a stronger decline of the specific organic matter mass than of the specific cell wall mass of plant parts at increasing temperature. Differences in whole tiller OMD between populations were observed only for vegetative tillers and were also caused by differences in CWC. It is concluded that temperature increase accelerated both the tiller development rate and the rate of decline of CWD during aging to the same extent, whereas plant parts responded similarly in the fractional CWD decline pattern as a function of DVS. These trends offer unique possibilities for modelling grass digestibility under contrasting temperature regimes. © 2003 Society of Chemical Industry