Synthesis,characterization and bioactivity investigation of bioglass/hydroxyapatite composite

Bioactive glass of the type CaO–P₂O₅–SiO₂ was obtained by the sol–gel processing method. The obtained material was characterized by X-ray powder diffraction (XRD). Composite samples of hydroxyapatite with synthesized bioglass were prepared at 1000 °C and characterized by XRD, Fourier transform infrared spectroscopy (FTIR), and surface electron microscopy (SEM). The bioactivity was examined in vitro with respect to the ability of hydroxyapatite layer to form on the surface as a result of contact with simulated body fluid (SBF). XRD, FTIR and SEM studies were conducted before and after contact of the material with SBF. It could be detected that the bioglass was crystallized partly. Furthermore, silicated hydroxyapatite may have formed due to the diffusion of silicate groups to the apatite phase and these may have substituted for the phosphate groups. It can be concluded from SEM and FTIR results that apatite phase formed after 14 days in SBF.     

Characterization,in vitro bioactivity and biological studies of sol-gel synthesized SrO substituted 58S bioactive glass

Bioactive glasses (BGs) are considered as a high potential candidate in bone repair and replacement. In the present study, sol–gel derived BGs based on 60% SiO₂-(36%-x) CaO-4%P₂O₅-x SrO (where x = 0, 5 and 10 mol%) quaternary system were synthesized and characterized. The effect of Sr substitutions on bioactivity, proliferation, alkaline phosphatase activity of osteoblast cell line MC3T3-E1 and antibacterial activity were investigated. Dried gels were stabilized at 700 °C to eliminate the nitrates and prevent the crystallization of bioactive glasses. X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy results confirmed the formation of hydroxycarbonate apatite on the BG surfaces. The 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and alkaline phosphate activity results showed that 5% SrO increased both differentiation and proliferation of MC3T3-E1 cells, while 10% SrO resulted in a decrease in bioactivity. Live/Dead and DAPI/Actin staining exhibited viable cell and the morphology of actin fibers and nuclei of MC3T3 cells treated with BG-0 and BG-5. The result of antibacterial test showed that strontium substituted 58S BG exhibited antibacterial effect against methicillin-resistant Staphylococcus aureus bacteria. Taken together, results suggest that 58S BG with 5 mol% SrO is a good candidate for bone tissue engineering with maximum cell proliferation and ALP activity, good bioactivity and high antibacterial efficiency.     

Bioactive Glass: An In-Vitro Comparative Study of Doping with Nanoscale Copper and Silver Particles

Bioactive glasses (BGs) based on 50SiO₂-45CaO-5P₂O₅ system doped with 1, 5, and 10 mol% CuO or Ag₂O were separately synthesized using quick alkali sol-gel method. Scanning electron microscope (SEM) analysis of the samples confirmed the formation of nano-sized BGs, whereas Fourier transform infrared (FTIR) spectra showed characteristic peaks for silica and phosphate groups. X-ray diffraction (XRD) pattern of the heat-treated (700°C) samples revealed the presence of crystalline metallic silver phase in all Ag-doped samples, while the XRD pattern of Cu-doped and control sample (50Si-45CaO-5P₂O₅) also heat-treated at 700°C confirmed their amorphous nature. Ultraviolet–visible (UV-Vis) studies along with Energy-dispersive X-ray spectroscopy (EDX) analysis confirmed the successful incorporation of Cu and Ag in bioglass. Antibacterial properties of the synthesized BGs were investigated by quantitative viable count method, and the results were related to the ion release profiles of the samples studied by flame atomic absorption spectroscopy (FAAS). Fast initial release of Ag observed in this study makes Ag-doped BG a better rapid bacteria-killing agent than Cu-doped BG, which exhibited a prolonged release of ions, suggesting that it may be a better candidate for long-term antibacterial protection.     

Preparation,characterization and in vitro biological response of simultaneous co-substitution of Zr+4/Sr+2 58S bioactive glass powder

In the present study, structure of zirconium-containing bioactive glass (58S-BG (Zr-BG)) with optimal fixed Zr content (5 mol.%) was modified by incorporation of strontium (Sr). These Zr and Sr-containing BGs (ZS-BGs) were synthesized by sol-gel method and substitution of Ca with modifier ions (Sr content = 0, 3, 6, 9, and 12 mol.%). The results obtained from characterization by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), and energy-dispersive X-ray spectroscopy (EDS) techniques from surface of all the ZS-BGs revealed formation of hydroxyapatite (HA) after 7 days of immersion in the simulated body fluid (SBF) solution. Evaluation of changes in the SBF solution, by monitoring pH variations and ions? concentration, was in agreement with the results of morphological and structural investigations. The in-vitro biological function of synthesized BGs was studied through (MTT) assay and alkaline phosphatase (ALP) activity analysis. The results showed that all the specimens significantly stimulated proliferation and viability of MC3T3 osteoblast-like cells. Furthermore, antibacterial studies confirmed less resistance of methicillin-resistant Staphylococcus aureus (MRSA) bacteria against ZS-BGs. Eventually, the results of in-vitro bio-analysis were clarified and confirmed by two cell staining techniques of Live/Dead and Dapi/Actin. This confirmation was achieved by observing the increased quantity of live cells and their nuclei as well as the decreased number of dead cells after co-culturing with all ZS-BGs.     

Preparation and characterisation of porous composite biomaterials based on silicon nitride and bioglass

The combination of bioinert and bioactive material offers new potentialities in bone tissue engineering. The present paper deals with preparation of novel biomaterial composite based on silicon nitride (Si₃N₄) and bioglass (in amount of 10 and 30 wt%) by free sintering at 980 °C for 1 h in nitrogen atmosphere. The obtained material was characterised by differential thermal analysis (DTA) and X-ray powder diffraction (XRD), porosity and pore size distribution were evaluated by means of mercury intrusion porosimetry (MIP). The bioactivity was examined in vitro with respect to the ability of hydroxyapatite layer formation on the surface of materials as a result of contact with simulated body fluid (SBF). All composites were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) before and after immersion in SBF. The bioglass-free sample was prepared as a reference material to compare the microstructure and bioactivity to the composites.     

The effect of magnesium content on in vitro bioactivity,biological behavior and antibacterial activity of sol–gel derived 58S bioactive glass

Bioactive glasses (BGs) have a great potential for bone replacement and regeneration in bone tissue engineering applications. In this research, first, sol–gel derived magnesium substituted 58?S BGs (MBGs) series composed of 60SiO₂–4P₂O₅-(36-x) CaO- xMgO, (x = 0; 1; 3; 5; 8 and 10?mol.%) were synthesized and stabilized at 700?°C to eliminate the nitrates and prevent the crystallization of MBGs. MgO was substituted for CaO in the BG formula up to 10?mol% and the effect of Mg concentration on in vitro bioactivity and cellular properties of the MBGs were investigated by immersing them in simulated body fluid (SBF) followed by structural characterization using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques. The effects Mg on proliferation and differentiation of osteoblastic MC3T3-E1 cells were also evaluated by 3-(4,5dimethylthiazol-2-yl)??2,5-diphenyltetrazolium bromide (MTT) and alkaline phosphate (ALP) activity.Results revealed that magnesium-substituted 58?S BG with 5?mol% MgO (BG-5) had the highest formation rate of hydroxyapatite (HA) while substitution of 8?mol% and10 mol% MgO (BG-8 and BG-10) lowered the bioactivity. MTT and ALP results confirmed that the substitution of the MgO up to 5?mol% increased both proliferation and differentiation of MC3T3-E1 cells, while more substitution had a negative effect and resulted in a decrease of proliferation and differentiation in BG-8 and BG-10. The result of antibacterial test showed that MBGs exhibited antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Taken together, results suggest that, among all the synthesized MBGs, sample BG-5 is a promising candidate as multifunctional biomaterial for bone tissue engineering with maximum cell proliferation and ALP activity, good bioactivity and high antibacterial efficiency against MRSA bacteria. Eventually, the BG-5 is suggested to be used in segmental defects in rat model in vivo.     

Porous chitosan/ZnO-doped bioglass composites as carriers of bioactive peptides

In this study, we aimed to assess whether the composite of chitosan/ZnO-doped bioglass can be applied as a suitable scaffold for the incorporation of bioactive peptides. Material of a porous composite with 1:1 ratio of bioglass:polymer was produced and used as a matrix for delivery of peptide. A peptide with the PEPTIDES sequence (Pro-Glu-Pro-Thr-Ile-Asp-Glu-Ser) was chosen as a model peptide. Microstructure and pore sizes of chitosan/ZnO-doped bioglass were assessed. Open porosity and pore sizes of the composite were suitable for enabling the migration of cells and ensuring the easy delivery of nutrients within the implant. In addition, composite showed bioactivity and bactericidal activity against Staphylococcus aureus and Pseudomonas aeruginosa strains. Peptide alone did not have any cytotoxic activity on human fibroblasts and keratinocytes. Also it did not show any antibacterial properties and did not cause hemolysis of red blood cells. The peptide incorporated in composite showed a rapid release in the kinetics profile. The obtained results indicate that there is the technological possibility to incorporate peptides in chitosan/ZnO-doped bioglass scaffolds. Such biomaterials have potential application in bone tissue engineering.     

Characterization,antibacterial and in vitro compatibility of zinc–silver doped hydroxyapatite nanoparticles prepared through microwave synthesis

We investigated the possibility of enhancing hydroxyapatite (HA) bioactivity by co-substituting it with zinc and silver. Zn–Ag–HA nanoparticles were synthesized by using the microwave-assisted wet precipitation process, and their phase purity, elemental composition, morphology, and particle size were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). FTIR, XRD, and EDX results showed the characteristic peaks of the Zn–Ag–HA structure, while SEM results demonstrated that the nanoparticles were of spherical shape with a particle size of 70–102 nm. Antibacterial tests of the nanoparticles revealed their antibacterial activity against Staphylococcus aureus and Escherichia coli. By using simulated body fluid (SBF), an apatite layer formation was observed at 28 days. In vitro cell adhesion assay confirmed the cell attachment of normal human osteoblast (NHOst) cells to the disc surface. MTT [(3(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide] assay indicated that the cells were viable, and the cells proliferated faster on the disks than on the control surface due to the presence of metal ions. In conclusion, the novel Zn–Ag–HA nanoparticles were found to be compatible with in vitro experiments and having potential antibacterial properties. Therefore these nanoparticles could be a promising candidate for future biomedical applications.     

Fabrication and characterization of 45S5 bioglass reinforced macroporous calcium silicate bioceramics

Bioactive and degradable macroporous bioceramics play an important role in clinical applications. In the present study, 45S5 bioglass reinforced macroporous calcium silicate ceramics (45BG-reinforced MCSCs) were fabricated. The effect of bioglass additives on compressive strength and open porosity of the samples was investigated, and the bioactivity and degradability of the obtained reinforced samples were also evaluated. The 45S5 bioglass additive was found to be effective to increase the strength of the MCSCs by the liquid-phase sintering mechanism. The optimum amount of bioglass additives was 5 wt.% and the compressive strength of the reinforced samples was approximately 2 times higher as compared to the pure macroporous calcium silicate ceramics (MCSCs). The compressive strength of the reinforced samples with about 50% porosity reached 112.47 MPa, which was similar to those of the cortical bones. After soaking in simulated body fluid (SBF), hydroxycarbonate apatite (HCA) layer was formed on the surface of the 45BG-reinforced MCSCs. Furthermore, the degradation rate of the reinforced samples was just about one-third of those pure MCSCs. Our results indicated that degradable 45BG-reinforced MCSCs possess excellent mechanical strength and bioactivity, and may be used as bioactive and degradable biomaterials for hard tissue prosthetics or bone tissue engineering applications.     

Structural behavior and in vitro bioactivity evaluation of hydroxyapatite-like bioactive glass based on the SiO2-CaO-P2O5 system

Silicate bioglass is of great importance in bone engineering because of its excellent bioactivity and osteogenic effects. In this study, hydroxyapatite-like bioactive glass based on the xSiO₂-CaO-P₂O₅ (x = 30, 45, 60 and 90 mol.%, Ca/P = 1.67) system was synthesized by the sol-gel method, and the corresponding structural evolution, apatite-forming ability and cytotoxicity were systematically investigated. The results suggest that both a higher heat treatment temperature and a lower SiO₂ content increase the crystallinity tendency of the bioglass, and the samples become obviously compact as the SiO₂ amount increases from 30 to 90 mol.%. Compared with the samples with higher SiO₂ content, the 30Si sample shows more remarkable internal connected mesoporous structures, with a higher specific surface area up to 129.12 m²/g, exhibiting excellent hydroxyapatite formation in simulated body fluid. Moreover, no obvious inhibitory effect was presented on human periodontal ligament cells (hPDLCs) for any of the silicate glass samples.     

Polysilazane-derived antibacterial silver–ceramic nanocomposites

Functional ceramic composites consisting of a dispersion of silver nanoparticles in a silicon (carbon)nitride matrix (nc-Ag/Si(C)N) were prepared via the polymer–ceramic route. Mixtures of 3 wt% as-synthesized Ag nanoparticles with a commercial polysilazane were pyrolysed under flowing nitrogen and/or ammonia. Bulk samples as well as coatings were investigated. Powder X-ray diffraction (XRD), transmission electron microscopy (TEM), thermal analysis (TGA, DTA), absorption spectroscopy (UV–vis) and infra red (IR) spectroscopy were used to characterize the products. The results indicate that the silver nanoparticles do not influence the cross-linking and pyroylsis process of the polysilazane precursor. At temperatures in the range of 800–1000 °C (H)Si(C)N matrices are obtained, which contain silver particles with an average size of 5–7 nm. Antibacterial tests on the pyrolysed material revealed strong activity against Escherichia coli and Staphylococcus aureus, suggesting the composites to be promising candidates for applications in fields such as the biomedical or food industries.     

Cerium-doped hydroxyapatite/collagen coatings on titanium for bone implants

The novelty of the present research consists in the possibility of obtaining cerium-doped hydroxyapatite/collagen coatings on the titanium support, to improve the performance of the bone implants. These coatings were deposited on the titanium surface by biomimetic method using a modified supersaturated calcification solution (SCS) additionally containing a cerium source and collagen. Prior to the deposition of the apatite layer, an alkali ÷ thermal oxidation pretreatment has been applied to ensure an increase in the bioactivity of the titanium surface. The coatings were examined by scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and Fourier transformed infrared (FTIR) spectroscopy. The EDX and XRD investigations of the coatings indicated that cerium was incorporated in the hydroxyapatite lattice. The collagen presence in the coatings was confirmed by FTIR analysis. The cerium-doped hydroxyapatite/collagen coatings showed good antibacterial efficacy against Escherichia coli and Staphylococcus aureus bacteria, being more effective against Escherichia coli. These coatings have a significant potential to be used in the dental and orthopedic implants, as the osseointegration depends on much more factors than simple formation of hydroxyapatite.     

Fabrication of corrosion resistant, bioactive and antibacterial silver substituted hydroxyapatite/titania composite coating on Cp Ti

The present work is aimed at developing a bioactive, corrosion resistant and anti bacterial nanostructured silver substituted hydroxyapatite/titania (AgHA/TiO₂) composite coating in a single step on commercially pure titanium (Cp Ti) by plasma electrolytic processing (PEP) technique. For this purpose 2.5 wt% silver substituted hydroxyapatite (AgHA) nanoparticles were prepared by microwave processing technique and were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM) methods. The as-synthesized AgHA particles with particle length ranging from 60 to 70 nm and width ranging from 15 to 20 nm were used for the subsequent development of coating on Cp Ti. The PEP treated Cp Ti showed both titania and AgHA in its coating and exhibited an improved corrosion resistance in 7.4 pH simulated body fluid (SBF) and 4.5 pH osteoclast bioresorbable conditions compared to untreated Cp Ti. The in vitro bioactivity test conducted under Kokubo SBF conditions indicated an enhanced apatite forming ability of PEP treated Cp Ti surface compared to that of the untreated Cp Ti. The Kirby-Bauer disc diffusion method or antibiotic sensitivity test conducted with the test organisms of Escherichia coli (E. coli) for 24 h showed a significant zone of inhibition for PEP treated Cp Ti compared to untreated Cp Ti.     

A high-efficacy and regenerable antimicrobial resin containing quaternarized N-halamine groups

A novel macroporous cross-linked antimicrobial resin containing quaternarized N-halamine groups, poly(1-chloro-5,5-dimethylhydantoinyl-3-ethyl-p-ethenylphenylmethyl dimethylammonium chloride) (Cl-PSQH), has been synthesized by a facile three-step reaction. The as-prepared resins were characterized by FT-IR, X-ray photoelectron spectroscopy, and zeta-potential measurement. Antimicrobial tests showed that Cl-PSQH was capable of about a 7-log inactivation of Staphylococcus aureus and Escherichia coli O157:H7 within 1 min of contact. Also, its regenerability and preliminary storage stability were investigated.     

Preparation and antibacterial activity of Ag/SiO2 thin film on glazed ceramic tiles by sol–gel method

In the present study, silver-doped silica thin films on glazed surface of ceramic tiles were well prepared by sol–gel method to achieve antibacterial activity. Thermal treatment was done in the air at 1100 °C for two hours. The Ag/SiO₂ thin films were investigated through Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and wavelength dispersive spectrometry (WDS). Atomic absorption spectroscopy (AAS) was used for the quantitative determination of the silver ion concentration being released from Ag/SiO₂ films over a 24 day period. The antibacterial effects of Ag/SiO₂ thin films against Escherichia coli and Staphylococcus aureus were also examined. From the analysis results, it was found that high temperature treated coating consists of two phases of SiO₂ and Ag based on the trapping of the Ag phase in the silica matrix. The presence of Ag elements on the surface of the coated tiles, were also observed. Thermal treatment at high temperatures caused sharp XRD peaks and high crystallinity in this system. Ag⁺ ions were released constantly and the mean release rate (±SD) was 0.104 ±0.01 μg/ml during 24 days. Coating films exhibited an excellent antibacterial performance against both bacterium.     

Novel bio-mediated Ag/Co3O4 nanocomposites of different weight ratios using aqueous neem leaf extract: Catalytic and microbial behaviour

An environmentally safe, economically, fast, and green synthetic approach was presented in this work for the fabrication of Ag-NP, Co₃O₄-NP and different weight ratio of Ag/Co₃O₄-NC. The synthesized samples were characterized by Fourier-transform infrared spectroscopy (FT-IR) and UV–visible spectrophotometry, Transmission Electron Microscopy (TEM), Scanning electron microscopy (SEM) along with X-ray energy dispersive spectroscopy (EDAX), and X-ray diffraction (XRD). The catalytic behaviour of the synthesized samples toward the reduction reaction of 4-nitrophenol in the presence of NaBH₄ was studied. It is indicated that the synthesized nanocomposites show a satisfactory catalytic activity. The catalytic reduction of p-NP to p-AP obeys first-order kinetics with rate constant in the following order (Ag-NP> (Ag/Co₃O₄-NC) ?1:1 > 1:3 > 3:1> Co₃O₄). This may be attributed to the high dispersion of Ag-NP compared to cobalt oxide nanoparticles. The microbial activity of the samples was analyzed using seven species of pathogenic microorganisms; 1) two species belongs to Gram negative bacteria; Escherichia coli (E.coli), and Salmonella, 2)four species belongs to Gram positive bacteria Marsa, Listeria, Staphylococcus aureus (S. aureus), bacillus subtilis (B.subtilis), and 3) one pathogenic fungal species Candidia. The data show that all samples exhibited an excellent range of inhibition toward all tested pathogenic microorganisms.     

Bioactivity and biodegradability of high temperature sintered 58S ceramics

Bioactive glasses are often considered in bone tissue engineering applications where mechanical strength is essential. As such, bioactive glass scaffolds are often sintered to improve mechanical strength. However, sintering can lead to crystallization, which reduces bioactivity and biodegradability. It has generally been considered that amorphous biomaterials exhibit better bioactivity. However, the in-vitro bioactivity and biodegradability of the sintered 58S made from initial amorphous powder and partially crystalline powder with the same chemical compositions (60SiO₂-36CaO-4P₂O₅ (mol%)) have not been compared before.In this study, 58S bioactive glass (fully amorphous) and glass-ceramic (partially crystallized) powders were synthesized using the sol-gel process, followed by heat-treating at 600 °C for 3 h (calcination). The powders were mixed with carboxymethyl cellulose solution as a binder, shaped in a cylindrical mold, dried, and then sintered at 1100 °C for 5 h. The in-vitro bioactivity and biodegradability of the sintered samples were assessed in simulated body fluid (SBF) for times up to 28 days. The specimens were investigated before and after immersion in SBF using X-ray powder diffraction (XRD), Scanning Electron Microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR). The In-vitro bioactivity and biodegradability rate of the sintered 58S produced from the glass ceramic powder were higher than that from fully amorphous powder. This study shows that the initial structure after calcination is important and affects the subsequent crystallization during sintering. Therefore, crystallinity and formation of hydroxyapatite after calcination are important controlling mechanisms that can increase the bioactivity and biodegradability rate of sintered 58S.     

Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract

In this study, silver nanoparticles were synthesized using the Crataegus douglasii fruit extract as a reducing agent. The reaction process was monitored by UV–vis spectroscopy. Further characterization was carried out using scanning electron microscopy (SEM). To optimize the biosynthesis of silver nanoparticles, the effect of process variables such as extract concentrations, mixing ratio of the reactants, time and pH were also investigated. The SEM images showed silver nanoparticles with 29.28 nm size and nearly spherical shape at 24 h interaction time. The antibacterial activity of the synthesized silver nanoparticles was confirmed against Staphylococcus aureus and Escherichia coli.     

Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

Here, we describe biosynthesis of silver nanoparticles by reduction of aqueous Ag⁺ ions with the culture supernatant of Pseudomonas aeruginosa. The morphological, physiological, biochemical and molecular level identification of the strain GS1 resembles P. aeruginosa. The nanoparticles synthesized by P. aeruginosa were characterized by UV–vis spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM). The size-distribution of nanoparticles was determined using a particle-size analyzer and the average particle-size was found to be 80 nm. The biological activities of the synthesized silver nanoparticles like antimicrobial activity were confirmed against Escherichia coli and Staphylococcus aureus and it have stable anti-coagulant effect.     

Strontium and copper co-substituted hydroxyapatite-based coatings with improved antibacterial activity and cytocompatibility fabricated by electrodeposition

Bacterial infection are serious complications for biomedical implants in the orthopedic and dental fields, and the ideal implants should combine good antibacterial ability and bioactivity. In this paper, we have fabricated the strontium/copper substituted hydroxyapatite (SrCuHA) coating on the commercially pure titanium (CP-Ti) and studied their effect on antibacterial and in vitro cytocompatible properties. Cu was incorporated into HA in order to improve its antimicrobial properties. Sr was added as a second binary element to improve the biocompatibility. The structural and morphological characteristics of the SrCuHA coatings were investigated using various analytical techniques. The presence of Sr²⁺ and Cu²⁺ in solution led to reduced roughness of the coating and finer nucleus size formed. The results highlight that Sr²⁺ and Cu²⁺ were homogenously incorporated into HA lattice to form SrCuHA coatings. Inductively coupled plasma mass spectrometry (ICP-MS) was used for the leach out analysis of the samples. A low contact angle value revealed the hydrophilic nature. In vitro electrochemical corrosion studies indicated that the SrCuHA coating sustain in the stimulated body-fluid (SBF), exhibiting superior corrosion resistance with a lower corrosion penetration rate than the bare CP-Ti substrate. The SrCuHA coatings can kill Escherichia coli to a certain extent during the first few days, which might be due to the Cu substitution in the coating. An enhancement of in vitro osteoblast adhesion, proliferation, and alkaline phosphatase activity was observed, which could lead to the optimistic orthopedic and dental applications.