Detectability of15N-depleted fertilizer N in soil and plant tissue during a corn-rye crop rotation

Use of¹⁵N-depleted fertilizer materials have been primarily limited to fertilizer recovery studies of short duration. The objective of this study was to determine if¹⁵N-depleted fertilizer N could be satisfactorily used as a tracer of residual fertilizer N in plant tissue and various soil N fractions through a corn (Zea mays L.) -winter rye (Secale cereale L.) crop rotation. Nitrogen as¹⁵N-depleted (NH₄)₂SO₄ was applied at five rates (0, 84, 168, 252, and 336 kg N ha^–1) to corn. Immediately following corn harvest a winter rye cover crop treatment was initiated. Residual fertilizer N was easily detected in the soil NO ₃ ^- -N fraction following corn harvest (140-d after application). Low levels of exchangeable NH ₄ ⁺ -N (<2.5 mg kg^–1) did not permit accurate isotope-ratio analysis. Fertilizer-derived N recovered in the soil total N fraction following corn harvest was detectable in the 0 to 30-cm depth at each N rate and in the 30 to 60 and 60 to 90-cm depths at the 336 kg ha^–1 N rate. Atom %¹⁵N concentrations in the nonexchangeable NH ₄ ⁺ -N fraction did not differ from the control at each N rate. Nitrogen recovery by the winter rye cover crop reduced residual soil NO ₃ ^- -N levels below the 10 kg ha^–1 level needed for accurate isotope-ratio analysis. Atom %¹⁵N concentrations in the soil total N fraction (approximately one yr after application) were indistinguishable from the control plots below the 168, 252, and 336 kg ha^–1 N rate at the 0 to 30, 30 to 60, and 60 to 90-cm depths, respectively. Recovery of residual fertilizer N by the winter rye cover crop was verified by measuring significant decreases in atom %¹⁵N concentrations in rye tissue with increasing N rates. The greatest limitation to the use of¹⁵N-depleted fertilizer N as a tracer of residual fertilizer N in a corn-rye crop rotation appears to be its detectibility from native soil N in the total N pool.Research partially supported by grants from the National Fertilizer and Environmental Research Center/TVA and the Virginia Division of Soil and Water Conservation.     

6. Ammonium dynamics of puddled soils in relation to growth and yield of lowland rice

The release of non-exchangeable (fixed) NH ₄ ⁺ and the importance of exchangeable NH ₄ ⁺ at transplanting (initial exchangeable NH ₄ ⁺ ) for rice (Oryza sativa L.) growth was studied in representative lowland rice soils of the Philippines.The experiments showed that initial exchangeable ammonium behaved like fertilizer N and thus may serve as a valuable guideline for nitrogen fertilizer application rates when calculated on a hectare basis. By using the¹⁵N tracer technique it was found that nonexchangeable ammonium in soil may contribute to the nitrogen supplying capacity of lowland rice soils. Fixation and release of NH ₄ ⁺ seem to be more dependent on the form of clay minerals than on clay content. In soils rich in vermiculite non-exchangeable ammonium should be considered together with other available N sources such as exchangeable ammonium for N fertilizer recommendations for lowland rice.     

Nitrogen fertilizer in leucaena alley cropping. II. residual value of nitrogen fertilizer and leucaena residues

Legume residues have been credited with supplying mineral nitrogen (N) to the associated cereal crop and improving soil fertility in the long term. Few studies using¹⁵N have reported the fate of legume N and fertilizer N in the presence of legume residues in soil-plant systems over periods of two years or longer. A field experiment was conducted in microplots to evaluate: (1) the residual value of the¹⁵N added in leucaena residues; (2) the residual value of fertilizer¹⁵N applied in the presence of unlabelled leucaena residues in the first year to maize over three subsequent years; and (3) the long-term fate of residual fertilizer and leucaena¹⁵N in a leucaena alley cropping system.There was a significant increase in maize production over three subsequent years after addition of leucaena residues. The residual effect of fertilizer N increased maize yield in the second year when N fertilizer was applied at 36 kg N ha^–1 in the first year in the presence of leucaena residues. Of the leucaena¹⁵N applied in the first year, the second, third and fourth maize crop recovered 2.6%, 1.8% and 1.4%, respectively. The corresponding values for the residual fertilizer¹⁵N were 0.7%, 0.4% and 0.3%. About 12–14% of the fertilizer¹⁵N added in the first year was found in the 200 cm soil profile over the following three years. This differed from the 38–41% of leucaena¹⁵N detected in the soil over the same period. Most of the residual fertilizer and leucaena¹⁵N in the soil was immobilized in the top 25 cm with less than 1% leached below 100 cm. More than 36% of the leucaena¹⁵N and fertilizer¹⁵N added in the first year was apparently lost from the soil-plant system in the first two years. No further loss of the residual leucaena and fertilizer¹⁵N was detected after two years.     

Nitrogen use efficiency,floodwater properties,and nitrogen-15 balance in transplanted lowland rice as affected by liquid urea band placement

Alternative N fertilizer management practices are needed to increase productivity and N use efficiency in lowland rice (Oryza sativa L.). In 1986 dry season, a field study using¹⁵N-labeled urea evaluated the effect of time and method of fertilizer N application on grain yield and N use efficiency. Conventional fertilizer application was compared with band placement of liquid urea and point placement of urea supergranules (USG). Grain yields were significantly higher with either band or point placement than with broadcast and incorporation or surface application. Partial pressure of NH₃ (NH₃) was significantly reduced when N was deep-placed.¹⁵N balance data show that fertilizer N applied basally and incorporated gave a total¹⁵N recovery of 52% and crop (grain + straw) recovery of 30%. Band placement of liquid urea N resulted in 82–90% total and 57–65% crop¹⁵N recovery. USG point placement gave 94% total and 70% crop¹⁵N recovery. Deep placement of second N application gave only slightly higher (98%)¹⁵N recovery compared with broadcast application (89%).     

Effect of climate on the recovery in crop and soil of15N-labelled fertilizer applied to wheat

Data was assembled from experiments on the fate of¹⁵N-labelled fertilizer applied to wheat (Triticum spp.) grown in different parts of the world. These data were then ranked according to the annual precipitation-evaporation quotient for each experimental location calculated from the average long-term values of precipitation and potential evaporation. Percentage recovery of¹⁵N fertilizer in crop and soil varied with location in accordance with the precipitation-evaporation quotient. In humid environments more¹⁵N fertilizer was recovered in the crop than in the soil, while in dry environments more¹⁵N fertilizer was recovered in the soil than in the crop. Irrespective of climatic differences between locations 20% (on average) of the¹⁵N fertilizer applied to wheat crops was unaccounted for at harvest. Most of the¹⁵N fertilizer remaining in the soil was found in the 0–30 cm layer. The most likely explanation of these differences is that wheat grown in dry environments has a greater root:shoot ratio than wheat grown in humid environments and, further, that the residue of dryland crops have higher C/N ratios. Both factors could contribute to the greater recovery of¹⁵N fertilizer in the soil in dry environments than in humid ones.     

Fate and efficiency of nitrogen fertilizers applied to wetland rice. II. Punjab,India

Two modified urea products (urea supergranules [USG] and sulfur-coated urea [SCU]) were compared with conventional urea and ammonium sulfate as sources of nitrogen (N), applied at 58 kg N ha^–1 and 116 kg N ha^–1, for lowland rice grown in an alkaline soil of low organic matter and light texture (Typic Ustipsamment) having a water percolation rate of 109 mm day^–1. The SCU and USG were applied at transplanting, and the whole dose of nitrogen was¹⁵N-labeled; the SCU was prepared in the laboratory and was not completely representative of commercial SCU. The SCU was broadcast and incorporated, whereas the USG was point-placed at a depth of 7–8 cm. The urea and ammonium sulfate applications were split: two-thirds was broadcast and incorporated at transplanting, and one-third was broadcast at panicle initiation. All fertilizers except the last one-third of the urea and ammonium sulfate were labeled with¹⁵N so that a fertilizer-N balance at flowering and maturity stages of the crop could be constructed and the magnitude of N loss assessed.At all harvests and N rates, rice recovered more¹⁵N from SCU than from the other sources. At maturity, the crop recovered 38 to 42% of the¹⁵N from SCU and only 23 to 31% of the¹⁵N from the conventional fertilizers, urea and ammonium sulfate, whose recovery rates were not significantly different. In contrast, less than 9% of the USG-N was utilized. Fertilizer nitrogen uptake was directly related to the yield response from the different sources. Most of the fertilizer N was taken up by the time the plants were flowering although recovery did increase up to maturity in some treatments.Analysis of the soil plus roots revealed that less than 1% of the added¹⁵N was in the mineral form. Between 20 and 30% of the¹⁵N applied as urea, SCU, and ammonium sulfate was recovered in the soil plus roots, mainly in the 0–15 cm soil layer. Only 16% of the¹⁵N applied as USG was recovered in the soil, and this¹⁵N was distributed throughout the soil profile to a depth of 70 cm, which was the lowest depth of sampling.Calculations of the¹⁵N balance showed that 46 to 50% of the urea and ammonium sulfate was unaccounted for and considered lost from the system. Only 27 to 38% of the¹⁵N applied as SCU was not recovered at maturity, but 78% of the USG application was unaccounted for. The extensive losses and poor plant recovery of USG at this site are discussed in relation to the high percolation rate, which is atypical of many ricegrowing areas.     

Fate of fertilizer nitrogen applied to wheat under simulated mediterranean environmental conditions

Triticum aestivumThe fate of fertilizer nitrogen applied to dryland wheat was studied in the greenhouse under simulated Mediterranian-type climatic conditions. Wheat, L., was grown in 76-cm-deep pots, each containing 50–70 kg of soil, and subjected to different watering regimes. Two calcareous clay soils were used in the experiments, Uvalde clay (Aridic Calciustoll) and Vernon clay (Typic Ustochrept). Fertilizer nitrogen balance studies were conducted using various¹⁵N-labeled nitrogen sources, including ammonium nitrate, urea, and urea amended with urea phosphate, phenyl phosphorodiamidate (a urease inhibitor), and dicyandiamide (a nitrification inhibitor). Wheat yields were most significantly affected by available water. With additional water during the growing period, the recovery of fertilizer nitrogen by wheat increased and the fraction of fertilizer nitrogen remaining in the soil decreased. In the driest regimes, from 40 to 65% of the fertilizer nitrogen remained in the soils. In most experiments the gaseous loss of fertilizer nitrogen, as estimated from unaccounted for¹⁵N, was not significantly affected by water regime. The¹⁵N not accounted for in the plant and the soil at harvest ranged from 12 to 25% for ammonium nitrate and from 12 to 38% for regular urea. Direct measurement of labeled ammonia loss from soil indicated that ammonia volatilization probably was the main N loss mechanism. Low unaccounted-for¹⁵N from nitrate-labeled ammonium nitrate, 4 to 10%, indicated that N losses due to denitrification, gaseous loss from plants, or shedding of anthers and pollen were small or negligible. Amendment of urea with urea phosphate to form a 36% N and 7.3% P product was ineffective in reducing N loss. Dicyandiamide did not reduce N loss from urea presumably because N was not leached from the sealed pots and denitrification was insignificant. Amendment of urea with 2% phenyl phosphorodiamidate reduced N loss significantly. However, band placement of urea at as 2-cm soil depth was more effective in reducing N loss than was amendment of broadcast urea with phenyl phosphorodiamidate.     

Uptake and balance of labelled fertilizer nitrogen by potatoes

Potatoes have a shallow rooting system. This can seriously affect the efficient use of fertilizer N. During two consecutive years, 1985 and 1986, a study was conducted on a commercial field to investigate the uptake of labelled N by potatoes under the recommended N rate and existing agricultural practices. The fertilizer efficiency, fertilizer distribution within the plant and soil and the total fertilizer balance were made using¹⁵NH₄ ¹⁵NO₃ 3.63 At. %¹⁵N excess. The recovery of the applied N-fertilizer in the whole plant was 25 and 56% for 1985 and 1986, respectively. The % Ndff and % Ndfs ranged between 30–40% and 60–70% respectively in both years. An important amount of fertilizer N was left in the soil after harvest. It reached 44 and 34% in 1985 and 1986, respectively.The total balance of the applied fertilizer N showed that up to 31 and 10% of the fertilizer N was lost during 1985 and 1986, respectively. The differences between the two growing seasons were mainly related to the method and timing of fertilizer N application and to the amount of rainfall.     

Recovery of 15N-labelled fertilizer applied to winter wheat and perennial ryegrass crops and residual 15N recovery by succeeding wheat crops under different crop residue management practices

The recovery of ¹⁵N-labelled fertilizer applied to a winter wheat (120 kg N ha^–1) and also a perennial ryegrass (60 kg N ha^–1) crop grown for seed for 1 year in the Canterbury region of New Zealand in the 1993/94 season was studied in the field. After harvests, ryegrass and wheat residues were subjected to four different residue management practices (i.e. ploughed, rotary hoed, mulched and burned) and three subsequent wheat crops were grown, the first succeeding wheat crop sown in 1994/95 to examine the effects of different crop residue management practices on the residual ¹⁵N recovery by succeeding wheat crops. Total ¹⁵N recoveries by the winter wheat and ryegrass (seed, roots and tops) were 52% and 41%, respectively. Corresponding losses of ¹⁵N from the crop-soil systems represented by un-recovered ¹⁵N in crop and soil were 12% and 35%, respectively. These losses were attributed to leaching and denitrification. The proportions of ¹⁵N retained in the soil (0-400 mm depth) at the time of harvest of winter wheat and ryegrass were 36% and 24%, respectively. Although the soil functioned as a substantial sink for fertilizer N, the recovery of this residual fertilizer by subsequent three winter wheat crops was low (1-5%) and this was not affected by different crop residue management practices.     

Nitrogen fertilizer in leucaena alley cropping. I. Maize response to nitrogen fertilizer and fate of fertilizer-15N

Field microplot experiments were conducted in the semi-arid tropics of northern Australia to evaluate the response of maize (Zea mays L.) growth to addition of N fertilizer and plant residues and to examine the fate of fertilizer¹⁵N in a leucaena (Leucaena leucocephala) alley cropping system, in which supplemental irrigation was used. Leucaena prunings, maize residues and N fertilizer were applied to alley-cropped maize grown in microplots which were installed in the alleys formed by leucaena hedgerows spaced 4.5 metres apart. The¹⁵N-labelled fertilizer was used to examine the fate of fertilizer N applied in the presence of mulched leucaena prunings and maize residues.Application of leucaena prunings increased maize yield while addition of N fertilizer in the presence of the prunings produced a further increase in maize production. There was a significant positive interaction between N fertilizer and leucaena prunings in increasing maize production. The addition of maize residues in the presence of N fertilizer and leucaena prunings decreased maize yield and N uptake and increased fertilizer¹⁵N loss from 38% to 47%. Maize recovered 24–79% of fertilizer¹⁵N in one cropping season, depending on application rate of N fertilizer and field management of plant residues. About 20–34% of fertilizer¹⁵N remained in the soil. More than 37% of fertilizer¹⁵N was apparently lost from the soil and plant system largely through denitrification when N fertilizer was applied at 40 kg N ha^–1 or more in the presence or absence of plant residues. Application of N fertilizer improved maize yield and increased the contribution of mulched leucaena prunings to crop production in the alley cropping system.     

The effect of autumn applied 15N-labelled fertilizer on nitrate leaching in a cultivated soil during winter

Nitrogen (N) fertilizer applied in autumn to arable farm land raises concerns over affects on ground water quality. The contribution of autumn ¹⁵N-labelled fertilizer (50 kg N ha^-1) to nitrate leaching losses from a cultivated soil (silt loam on sandy loam; Udic Ustochrept) was measured using undisturbed monolith lysimeters (500 mm diameter, 700 mm long) during consecutive winters in Canterbury, New Zealand. The addition of ¹⁵N-labelled fertilizer at 50 kg N ha^-1 did not significantly increase nitrate leaching losses. Soil-derived-N contributed 78 and 88% (1996 and 1997, respectively) of the nitrate leached beneath fertilized lysimeters. Warmer weather and wetter soil conditions at cultivation and fertilizer application during 1997, compared with 1996, resulted in an increased release of soil-derived-N in 1997. Nitrate leaching and average nitrate concentrations were therefore 41% and 56% higher, respectively, during the winter of 1997 than the winter of 1996. However, fertilizer leaching losses were relatively consistent between years (7.8 and 8.6%). Although not statistically significant, total N leaching losses and average nitrate concentration were 24 to 30% higher below fertilized lysimeters as compared with unfertilized lysimeters, indicating a priming effect of fertilizer on soil N release. During both late winter periods, leachate nitrate concentrations from fertilized and unfertilized lysimeters exceeded World Health Organisation (WHO) limits for drinking water. Higher release of soil-derived-N in 1997 also meant WHO limits were exceeded for 6 weeks longer than in 1996. In conclusion, the application of ¹⁵N fertilizer in autumn directly contributed only a small proportion of the total amount of N leached in this cultivated soil. However, the apparent priming effect of autumn applied-N fertilizer has importance on the overall environmental impact of this production system, as the amount of N leached, and extent to which health limits were exceeded, was largely determined by the factors which controlled the release of soil-derived-N.     

Uptake of fertilizer and soil nitrogen by ryegrass swards during spring and mid-season

Double-labelled¹⁵N ammonium nitrate was used to determine the uptake of fertilizer and soil N by ryegrass swards during spring and mid-season. The effects of water stress (40% of mean rainfall v 25 mm irrigation per 25 mm soil water deficit) and the rate of application of N in the spring (40 v 130 kg ha^–1) on the recovery of 130 kg N ha^–1 applied in mid-season were also evaluated. Apparent recovery of fertilizer N (uptake of N in the fertilized plot minus that in the control expressed as a percentage of the N applied) was 95 and 79% for fertilizer N applied in the spring at rates of 40 and 130 kg ha^–1, respectively. Actual recovery of the fertilizer N assessed from the uptake of¹⁵N was only 31 and 48%, respectively. The uptake of soil N by the fertilized swards was substantially greater than that by the control. However, the increased uptake of soil N was always less than the amount of fertilizer N retained in or lost from the soil. Broadly similar patterns for the uptake of fertilizer and soil N were observed during mid-season. Uptake of N in mid-season was highest for swards which received 40 kg N ha^–1 in the spring and suffered minimal water stress during this period. Application of 130 kg N ha^–1 in spring reduced the uptake of N in mid-season to an extent similar to that arising from water stress. Only 1.8 to 4.2 kg ha^–1 (3 to 10%) of the N residual from fertilizer applied in the spring was recovered during mid-season. Laboratory incubation studies suggested that only a small part of the increased uptake of soil N by fertilized swards could be attributed to increased mineralisation of soil N induced by addition of fertilizer. It is considered that the increased uptake of soil N is partly real but mostly apparent, the latter arising from microbially mediated exchange of inorganic¹⁵N in the soil.     

Influence of delaying flood and preflood nitrogen application on dry-seeded rice

In the southern U.S. rice belt it is recommended that rice (Oryza sativa L.) grown in the dry-seeded, delayed flood cultural system have the preflood N fertilizer applied and the field flooded at the fourth to fifth leaf stage of plant development. The objective of this field study was to determine if delaying the flood and preflood N application past the fifth leaf stage was detrimental to rice total N and fertilizer¹⁵N uptake, total dry matter, and grain yield. This study was conducted on a Crowley silt loam (Typic Albaqualfs) and a Perry clay (Vertic Haplaquepts). The preflood N fertilizer and flood were delayed 0, 7, 14, or 21 d past the fourth to fifth leaf stage, after which time a permanent flood was established and maintained until maturity. All treatments received 20.5 g N m^–2 as¹⁵N-labeled urea in three topdress applications. All plant and soil samples were taken at maturity. Harvest index increased as the preflood N and flood were delayed past the 4 to 5 leaf stage. Total N in the grain + straw either decreased or showed a decreasing trend as the N and flood were delayed. Similarly, uptake of native soil N decreased as flood was delayed. Conversely, percent recovery of fertilizer N in the rice plant and the plant-soil system increased as the preflood N and flood were delayed. Rice grain yield was not significantly affected by delaying the preflood N and flood up to 21 d.Received....... . Published with permission of the Director of the Arkansas Agric. Exp. Stn. Project ARK01386. Supported in part by the Tennessee Valley Authority National Fertilizer and Environmental Research Center and the Arkansas Rice Research and Promotion Board.     

Intercropping of Wheat and Pea as Influenced by Nitrogen Fertilization

The effect of sole and intercropping of field pea (Pisum sativum L.) and spring wheat (Triticum aestivum L.) on crop yield, fertilizer and soil nitrogen (N) use was tested on a sandy loam soil at three levels of urea fertilizer N (0, 4 and 8 g N m⁻²) applied at sowing. The ¹⁵ N enrichment and natural abundance techniques were used to determine N accumulation in the crops from the soil, fertilizer and symbiotic N₂ fixation. Intercrops of pea and wheat showed maximum productivity without the supply of N fertilizer. Intercropping increased total dry matter (DM) and N yield, grain DM and N yield, grain N concentration, the proportion of N derived from symbiotic N₂ fixation, and soil N accumulation. With increasing fertilizer N supply, intercropped and sole cropped wheat responded with increased yield, grain N yield and soil N accumulation, whereas the opposite was the case for pea. Fertilizer N enhanced the competitive ability of intercropped wheat recovering up to 90% of the total intercrop fertilizer N acquisition and decreased the proportion of pea in the intercrop, but without influencing the total intercrop grain yield. As a consequence, Land Equivalent Ratios calculated on basis of total DM production decreased from a maximum of 1.34 to as low as 0.85 with increased fertilizer N supply. The study suggests that pea–wheat intercropping is a cropping strategy that use N sources efficiently due to its spatial self-regulating dynamics where pea improve its interspecific competitive ability in areas with lower soil N levels, and vice versa for wheat, paving way for future option to reduce N inputs and negative environmental impacts of agricultural crop production.     

Effect of the timing and rate of nitrogen fertilization on the growth and recovery of fertilizer nitrogen within the potato (Solanum tuberosum L.) crop

The effect of the timing of N fertilizer application on the uptake and partitioning of N within the crop and the yield of tubers has been studied in two experiments. In 1985 either none, 8 or 12 g N m^–2 was applied and in 1986 none, 12 or 18 g N m^–2. Fertilizer N was applied either at planting, around the time of tuber initiation or half at planting and the remainder in four foliar sprays of urea during tuber bulking.¹⁵N-labelled fertilizer was applied to measure the recovery of fertilizer N in the crops.There was an apparent pre-emergence loss of nitrate from the soil when N was applied at planting in 1986, thereby reducing the efficiency of fertilizer use. Applying the N at tuber initiation delayed and reduced the accumulation of N in the canopy compared with crops receiving all their fertilizer at planting. Foliar sprays of urea slightly increased both tuber yields and tuber N contents when compared to a single application at planting. The proportion of the fertilizer N recovered in the crop was little affected by the rate of N application, but a greater proportion of foliar-applied N was recovered than N broadcast at planting, due partly to pre-emergence losses of nitrate in 1986. It is suggested that late applications of N was foliar sprays can be of benefit to crops with a long growing season and reduce environmental losses of N.     

Sugarcane residue management and grain legume crop effects on N dynamics,N losses and growth of sugarcane

To reduce greenhouse gas emissions farmers are being encouraged not to burn sugarcane residues. An experiment was set up in NE Thailand, where sugarcane residues of the last ratoon crop were either burned, surface mulched or incorporated and subsequently the field left fallow or planted to groundnut or soybean. The objectives of the current experiment were to evaluate the residual effects of these treatments during the following new sugarcane crop on (i) microbial and mineral N dynamics, (ii) performance of sugarcane and (iii) effectiveness of recycled legume residues compared to mineral N fertilizer on N use efficiencies, ¹⁵N recovery in the system and in soil particle size and density fractions (using ¹⁵N labelled legume residues and fertilizer). The millable cane and sugar yield were positively affected by sugarcane residue mulching and incorporation compared to burning suggesting microbial remobilization of previously immobilized N. Residual effects of legumes increased sugarcane tillering and yield (127 and 116 Mg ha⁻¹ for groundnut and soybean, respectively) compared to the fallow treatment without N fertilizer (112 Mg ha⁻¹). Soybean residues of higher C:N ratio (33:1) and lignin content (13%) compared to groundnut residues (21:1 C:N, 5% lignin) decomposed slower and improved N synchrony with cane N demand. This led to a better conservation of residue N in the system with proportionally less ¹⁵N losses (15–17%) compared to the large losses from groundnut residues (50–57%) or from mineral N fertilizer (50–63%). ¹⁵N recoveries in soil were larger from residues (41–80%) than from fertilizer (30%) at final harvest. Recycled legume residues were able to substitute basal fertilizer N application but not topdressing after 6 months.     

Balance sheet of15N labelled urea applied to rice in three Australian vertisols differing in soil organic carbon

A glasshouse experiment was conducted to study the balance sheet of¹⁵N labelled urea at three rates (zero, 31.48 and 62.97 mmol N pot^–1) applied to rice under flooded conditions with two moisture regimes (continuous and alternate flooding) using three Australian vertisols differing in organic carbon level. Walkley-Black organic carbon values for the three soils were 0.65, 2.13 and 3.76 for the low carbon (LC), medium carbon (MC) and high carbon (HC) soils respectively.Rice dry weight and nitrogen uptake was significantly affected by N fertilizer rates, water regimes and soils. Alternate flooding gave much lower dry weight and nitrogen uptake than continuous flooding and the LC soil gave lower dry weight and nitrogen uptake than for the MC and HC soils.Recovery of¹⁵N labelled urea fertilizer in the rice plant was low (15.4 to 38.4%) and the¹⁵N urea not accounted for in the plant or soil and presumed lost was high (36.2 to 76.0%). Recovery was lower and loss higher under alternate flooding and for the LC soil. There was no effect of fertilizer rate. The results obtained stress the need for careful management to reduce losses of nitrogen fertilizer, particularly for soils low in organic carbon.     

The effect of plant residues on plant uptake and leaching of soil and fertilizer nitrogen in a tropical red earth soil

Two field experiments, in which differing amounts and types of plant residues were incorporated into a red earth soil, were conducted at Katherine, N.T., Australia. The aim of the work was to evaluate the effect of the residues on uptake of soil and fertilizer N by a subsequent sorghum crop, on the accumulation and leaching of nitrate, and on losses of N.Stubble of grain sorghum applied at an exceptionally high rate (~ 18 000 kg ha^–1) reduced uptake of N by sorghum by 13% and depressed the accumulation of nitrate under a crop and particularly under a fallow.Loss of fertilizer N, movement of nitrate down the profile, and uptake by the crop was studied in another experiment after application of N as¹⁵NH₄ ¹⁵NO₃ to field microplots. By four weeks after fertilizer application 14% had been lost from the soil-plant system and by crop maturity 36 per cent had been lost. The pattern of¹⁵N distribution in the profile suggested that losses below 150 cm had occurred during crop growth. The recovery of¹⁵N by the crop alone ranged from 16 to 32 per cent. There was an apparent loss of N from the crop between anthesis and maturity. Residue levels common to sorghum crops in the region (~ 2000 kg ha^–1) did not significantly affect uptake by a subsequent sorghum crop, N losses, or distribution of nitrate in the profile.     

Movement and distribution of fertilizer nitrogen as affected by depth of placement in wetland rice

Experiments were conducted to monitor the movement and distribution of ammonium-N after placement of urea and ammonium sulfate supergranules at 5, 7.5, 10, and 15 cm. By varying depths of fertilizer placement, it is possible to determine the appropriate depth for placement machines. There were no significant differences in grain yields with nitrogen placed 5 and 15 cm deep. However, grain yields were significantly higher with deep placement of nitrogen than with split application of the fertilizer. The lower yields with split-applied nitrogen were due to higher nitrogen losses from the floodwater. The floodwater with split application had 78–98µg N ml^–1 and that with deep-placed nitrogen had a negligible nitrogen concentration.Movement of NH ₄ ⁺ -N in the soil was traced for various depths after fertilizer nitrogen application. The general movement after deep-placement of the ammonium sulfate supergranules was downward > lateral > upward from the placement site. Downward movement was prevalent in the dry season: fertilizer placed at 5–7.5 cm produced a peak of NH ₄ ⁺ -N concentration at 8–12 cm soil depth; with placement at 15 cm, the fertilizer moved to 12–20 cm soil depth. Fertilizer placed at 10 cm tended to be stable. In the wet season, deep-placed N fertilizer was fairly stable and downward movement was minimal.A substantially greater percentage of plant N was derived from¹⁵N-depleted fertilizer when deep-placed in the reduced soil layer than that applied in split doses. The percent N recovery with different placement depths, however, did not vary from each other. The results suggest that nitrogen placement at a 5-cm soil depth is adequate for high rice yields in a clayey soil with good water control. In farmers' fields where soil and water conditions are often less than ideal, however, it is desirable to place nitrogen fertilizer at greater depths and minimize NH ₄ ⁺ -N concentration in floodwater.     

A microplot study of the fate of15N-labelled ammonium nitrate and urea applied at two rates to ryegrass in spring

Confined microplots were used to study the fate of¹⁵N-labelled ammonium nitrate and urea when applied to ryegrass in spring at 3 lowland sites (S1, S2 and S3). Urea and differentially and doubly labelled ammonium nitrate were applied at 50 and 100 kg N ha^–1. The % utilization of the¹⁵N-labelled fertilizer was measured in 3 cuts of herbage and in soil to a depth of 15 cm (soil_0–15).Over all rates, forms and sites, the % utilization values for cuts 1, 2, 3 and soil_0–15 were 52.4, 5.3, 2.4 and 16.0% respectively. The % utilization of¹⁵N in herbage varied little as the rate of application increased but the % utilization in the soil_0–15 decreased as the rate of application increased. The total % utilization values in herbage plus soil_0–15 indicated that losses of N increased from 12 to 25 kg N ha^–1 as the rate of N application was increased from 50 to 100 kg N ha^–1.The total % utilization values in herbage plus soil_0–15 over both rates of fertilizer N application were 84.1, 80.8 and 81.0% for urea compared with 74.9, 72.5 and 74.4% for all ammonium nitrate forms at S1, S2 and S3 respectively. Within ammonium nitrate forms, the total % utilization values in herbage plus soil_0–15 over both rates and all sites were 76.7, 69.4 and 75.7% for¹⁵NH₄NO₃, NH₄ ¹⁵NO₃ and¹⁵NH₄ ¹⁵NO₃ respectively. The utilization of the nitrate moiety of ammonium nitrate was lower than the utilization of the ammonium moiety.The distribution of labelled fertilizer between herbage and soil_0–15 varied with soil type. As the total utilization of labelled fertilizer was similar at all sites the cumulative losses due to denitrification and downward movement appeared to account for approximately equal amounts of N at each site.