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1.
ABSTRACT: The use of antimicrobial ingredients in combination with irradiation is an effective antilisterial intervention strategy for ready-to-eat meat products. Microbial safety was evaluated for frankfurters formulated with 0% or 3% added potassium lactate/sodium diacetate solution and inoculated with Listeria monocytogenes before or after treatment with irradiation (0, 1.8, or 2.6 kGy). Frankfurters were stored aerobically or vacuum packaged and L. mo nocytogenes counts and APCs were determined while refrigerated. The incorporation of lactate/diacetate with or without irradiation had a strong listeriostatic effect for aerobically stored frankfurters. Outgrowth was suppressed and counts were not different from initial counts (5.2 log CFU/frank compared with 5.0 log CFU/frank); however, those without the additive increased steadily (5.4 to 9.3 log CFU/frank). Irradiation treatments alone had higher L. monocytogenes counts after 3 wk. For vacuum-packaged frankfurters, both the addition of lactate/diacetate and irradiation were effective at controlling growth after 8 wk. Large and incremental reductions in total counts were seen for irradiation treatments. Initial counts were reduced by 3 log CFU with the application of 1.8 kGy while 2.6 kGy decreased counts over 5 log CFU. These reductions were maintained throughout storage for lactate/diacetate-treated frankfurters. By 8 wk, L. monocytogenes counts on 1.8 and 2.6 kGy irradiated frankfurters without lactate/diacetate increased to 7.43 and 6.13 log CFU, respectively. Overall, lactate/diacetate retarded the outgrowth of L. monocytogenes on frankfurters throughout aerobic storage and the combination of irradiation and 3% lactate/diacetate reduced and retarded growth of L. monocytogenes , especially during the last 2 wk of vacuum-packaged storage. 相似文献
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ABSTRACT: Listeria monocytogenes , a psychrotrophic foodborne pathogen, is a recurring postprocess contaminant on ready-to-eat meat (RTE) products, including frankfurters. Potassium lactate (PL) and sodium diacetate (SDA) are FDA-approved antimicrobials that inhibit the growth of L. monocytogenes when incorporated into the formulation of fine emulsion sausage. Flash (steam) pasteurization (FP) has been shown to reduce levels of L. monocytogenes , and its surrogate L. innocua , on frankfurter surfaces. The ability of FP to inactivate and prevent the growth of the L. monocytogenes surrogate L. innocua in a pilot plant setting was investigated. FP treatment (1.5 s, 121 °C) of single layers of frankfurters that were surface-inoculated with either 5, 4, or 3 log CFU/g of L. innocua immediately before FP (1.5 s, 121 °C) resulted in log reductions of 1.97 (± 0.11), 2.03 (± 0.10), or 2.07 (± 0.14), respectively. Inoculum level had no effect on the inactivation of L. innocua . Following 8 wk of refrigerated storage (4 °C), L. innocua levels decreased by 0.5 log in non-FP-treated frankfurter packs, while the 2 log reduction of L. innocua was maintained for FP-treated frankfurters. FP (1.5 s, 121 °C) had no effect on frankfurter color or texture. Because the numbers of L. monocytogenes associated with contaminations of ready-to-eat meats are typically very low, the use of FP in combination with PL and SDA has the potential to reduce the number of frankfurter recalls and foodborne illness outbreaks. 相似文献
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E.M. Martin C.L. Griffis K.L.S. Vaughn C.A. O'Bryan E.C. Friedly J.A. Marcy S.C. Ricke P.G. Crandall R.Y. Lary Jr. 《Journal of food science》2009,74(6):M237-M241
ABSTRACT: Listeria monocytogenes (Lm) is a food safety concern that can be associated with ready-to-eat (RTE) meat and poultry products because of its persistence in the processing environment. Listeriosis has a fatality rate of 28% in immuno-compromised individuals. RTE meats receive a lethal heat treatment but may become contaminated by Lm after this treatment. Federal regulators and manufacturers of RTE meats are working to find additional ways to control postprocess contamination by Lm in RTE meats. This research was initiated to validate combinations of antimicrobials that would produce an immediate lethality of at least 1 log of Lm on artificially contaminated frankfurters, and also suppress Lm growth to less than 2 logs throughout the extended shelf life at refrigerated temperatures (4 °C). Based on our studies, 22-ppm lauric arginate (LAE, ethyl-N-dodecanoyl-L-arginate hydrochloride) gave more than a 1-log reduction of Lm surface inoculated onto frankfurters within 12 h. The combination of either 1.8%/0.13% or 2.1%/0.15% potassium lactate/sodium diacetate (L/D) in combination with 22 ppm LAE caused more than a 2-log reduction at 12 h. Storage studies revealed that complementary interactions of L/D and LAE also met the 2nd requirement. This combination initially reduced Lm by 2 logs and suppressed growth to less than 2 logs even at the end of the 156-d storage life for frankfurters. These results confirmed that the combination of L/D with LAE as a postprocessing–prepackaging application could be useful in complying with the USDA's Alternative 1 that requires validation for the control of Lm on RTE frankfurters. 相似文献
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J-F. MEULLENET H.C. CHANG J.A. CARPENTER A.V.A. RESURRECCION 《Journal of food science》1994,59(4):729-733
Chicken frankfurters made with 0, 2, 4, 6, 8% added collagen fibers and 10, 15, 20, 25, 30% added water were evaluated for textural differences using a torsion test and sensory texture profile analysis. Frankfurters with high amounts of added water and low amounts of collagen fibers had lower shear stress values. Sensory analysis indicated that both collagen fibers and added water had significant influences (P<0.05) on hardness, springiness and juiciness. The addition of collagen fibers resulted in harder, springier, and less juicy frankfurters. Added water resulted in softer, less springy and juicier frankfurters. Response surface methodology demonstrated that for 2% added collagen, the optimum added water was ～20%. 相似文献
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M.J. Zhu A. Mendonca B. Min E.J. Lee K.C. Nam K. Park M. Du H.A. Ismail D.U. Ahn 《Journal of food science》2004,69(5):C382-C387
ABSTRACT: Breast rolls with 6 antimicrobial additive treatments—no preservatives (control), 0.1% potassium benzoate (PB), 2% sodium lactate (SL), 0.1% potassium benzoate plus 2% sodium lactate (PB + SL), 2% sodium lactate plus 0.1% sodium diacetate (SL + SDA), and 0.1% potassum benzoate, 2% sodium lactate, and 0.1% sodium diacetate (PB + SL + SDA)—were prepared. Samples were irradiated at 0, 1.0, or 2.0 kGy, and then the quality characteristics of turkey rolls were analyzed. Adding 2% SL increased the hardness, springiness, cohesiveness, chewiness, and resilience of breast rolls. Addition of PB or SDA, and irradiation had no significant effect on texture. Adding 2% SL affected color values. The color a * and b * values of turkey rolls with 2% SL added were significantly lower than those of the control, and this difference was maintained after irradiation and during storage. No difference in color and texture was observed between turkey rolls added with SL and those added with SL + PB + SDA. Breast rolls containing antimicrobials had more lipid oxidation than control. Irradiation and storage slightly enhanced lipid oxidation, although the overall lipid oxidation was very low. Irradiation promoted the formation of dimethyl disulfide and dimethyl trisulfide. Adding PB in breast rolls greatly increased the formation of benzene during irradiation, whereas other antimicrobial additives had no significant effects on volatiles. 相似文献
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ABSTRACT: The objective of this study was to evaluate the effects of enhancement solutions containing sodium lactate or sodium lactate/sodium diacetate on E. coli K12 transmission to beef strip steaks and in purge. Solutions containing salt, phosphate, and shelf-life enhancers were injected to 10% over initial weight of the steaks. Lactate or lactate/diacetate addition to a solution inoculated with 6 log10 E. coli K12 CFU/mL was equally effective in limiting growth resulting in a 1-2 log10 CFU/g reduction when compared to salt/phosphate in steaks. When inoculation level was 3 log10 CFU/mL in the enhancement solution, microbial growth was detectable only in the purge of steaks enhanced with salt/phosphate only. Lactate increased CIE L* value and b* and decreased a* values when compared to the control. Lactate-/diacetate-containing solutions decreased L* values when compared to lactate alone. Lactate/diacetate also reduced purge loss from steaks compared to lactate and salt/phosphate solutions. 相似文献
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Different organogel formulations used as beef fat (BF) replacement (0%, 20%, 40%, 60%, and 80%) were utilized to optimize the mechanical properties of frankfurters. Organogels, made of canola oil (CO), included different concentrations of ethyl cellulose (EC) and sorbitan monostearate (SMS). They consisted of: 8% EC + 1.5% SMS referred to as organogel‐I (OG‐I), 8% EC + 3.0% SMS (OG‐II), and 10% EC + 1.5% SMS (OG‐III), which were found promising in a previous study when used at 100% replacement. Replacement of BF with organogels at all levels could bring down the very high hardness values (texture profile analysis and sensory) of frankfurters prepared using CO by itself, relative to the BF control. OG‐I and OG‐II quantity had no significant effect on hardness and springiness, being similar in many cases to the BF and lower than the CO control. Shear force values of all organogel treatments were not significantly different from one another, and were between the BF and CO controls. Smokehouse yield showed a pattern of decreasing losses with increasing organogel replacement level. Sensory analysis revealed that using CO by itself significantly increased hardness, but structuring the oil (via organogelation), brought it down to the BF control value in all OG‐I and OG‐II formulations. Juiciness was significantly reduced by using liquid oil but increased with raising the amount of organogels. Oiliness sensation increased with higher organogel substitution and was actually higher than the beef control. The study demonstrates the potential use of vegetable oil structuring in replacing the more saturated BF in emulsion‐type meat products. 相似文献
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Terry A. Houser Joseph G. Sebranek Wigberto Núñez Maisonet Joseph C. Cordray Bryon R. Wiegand Dong Ahn Eun J. Lee 《Journal of food science》2005,70(4):S262-S266
ABSTRACT: Commercially produced sliced ham and all-pork frankfurters were obtained from a national meat processor and irradiated at 1.6 kGy. The samples were evaluated for color, lipid oxidation, odor, flavor, and the production of volatiles over an 8-wk storage period. Irradiation processing did not affect color values for the ham or frankfurters. Lipid oxidation as measured by 2-thiobarbituric acid-reactive substances (TBARS) did not increase for either the ham or frankfurters. Irradiation processing increased off-odor scores for the ham but not for frankfurters. On the other hand, off-flavor scores were not significantly different for ham but were higher in frankfurters after irradiation processing. Dimethyl disulfide content increased as a result of irradiation in both the ham and frankfurters but decreased during the 8-week storage period. Irradiation processing resulted in the formation of new volatile compounds in the ham samples including heptane, trans -1-butyl-2-methylcyclopropanone, 2-octene, and toluene, which were not present in nonirradiated ham. In the case of frankfurters, irradiation treatment resulted in the formation of 2-butanone, which was not present in the nonirradiated frankfurters. Most volatile compounds that were affected by irradiation processing of either the ham or frankfurters were increased when compared with nonirradiated controls. Although color and lipid oxidation (TBARS) did not seem to be affected by irradiation processing at 1.6 kGy, changes in odor, flavor, and the production of volatiles are of concern if irradiation is to be used to control microbial growth in ready-to-eat pork products. 相似文献
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Chicken frankfurters fat content was decreased by using various levels of oat bran and added water and evaluated for quality. Frankfurters with higher levels of oat bran had less expressible moisture and required higher shear stress to break. There were no difference in springiness among frankfurters made with different levels of oat bran; however, those with higher oat bran were rated less juicy and more grainy by taste panelists. The addition of water increased intensities of strain, springiness and juiciness, but decreased shear stress and hardness values. Contour plots showed optimum formulation at <2% oat bran 20% of added water. 相似文献
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Calcium Fortified, Reduced Fat Beef Emulsion Product 总被引：1，自引：0，他引：1
Characteristics were investigated on a 15%/25%, fat/added water beef frankfurter supplemented with calcium (calcium carbonate or calcium-citrate-malate complex, CCM) to meet 25, 50, 75 or 100% of adult U.S. RDA in one 45g frankfurter. Controls contained 15%/25% or 30%/10% fat/added water. Compared to controls, calcium addition did not reduce yield although batters containing CCM had lower viscosity (P<0.05). During storage, pH of calcium added frankfurters increased about 0.35 units. Frankfurters formulated with 100% levels for calcium were least acceptable to sensory panelists. Frankfurters were softer and had less springiness and chewiness (P<0.05) when supplemented with 100% levels for calcium. 相似文献
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利用超微冷榨粉碎火麻粕（micronized cold-pressed hemp meal,MCPHM）替代传统的磷酸盐,探究MCPHM添加量（1%、2%、3%、4%、5%,m/m）对低磷（50%磷酸盐）法兰克福香肠品质及感官特性的影响。结果表明,MCPHM可作为理想的磷酸盐替代品,随着MCPHM添加量的增加,低磷法兰克福香肠的蒸煮损失、亮度值和红度值显著降低（P＜0.05）。与此同时,低磷法兰克福香肠的乳化稳定性、黄度值、切片致密性和硬度、弹性等则随着MCPHM添加量的增加而显著增加（P＜0.05）。然而,较高的MCPHM添加量显著降低了香肠的多汁性、风味、硬度、弹性、回复性、咀嚼性、脆性和内聚性（P＜0.05）。综上所述,以4%的MCPHM替代50%的磷酸盐的效果最佳,可提高低磷法兰克福香肠的品质特性。因此,将MCPHM替代磷酸盐可作为改善低磷法兰克福香肠质量缺陷的一种实用方法。 相似文献
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法兰克福香肠在生产、运输、贮藏过程中容易产生析水、析油等问题,影响香肠的食用品质,因此提高法兰克福香肠的品质特性一直是肉制品加工研究的重点。本研究探究不同黄芪胶添加量（0、0.1%、0.2%、0.3%、0.4%、0.5%,m/m）对法兰克福香肠品质特性的影响。结果表明,添加黄芪胶能够显著降低法兰克福香肠的蒸煮损失（P＜0.05）,并显著提高其乳化稳定性（P＜0.05）。与此同时,随着黄芪胶添加量的增加,法兰克福香肠的硬度、弹性、脆性、亮度值呈先上升后降低的趋势,且在添加量为0.3%时达到最大（P＜0.05）。动态流变学测试结果表明,添加黄芪胶能够提高肉糜的储能模量（G’）和损失模量（G”）,而且降低损耗角正切值（tanδ）,从而提高肉糜的黏弹性。扫描电镜结果表明,黄芪胶添加能够明显改善香肠凝胶的组织状态,形成致密且均匀的三维凝胶网络结构。综上所述,添加黄芪胶能够显著改善法兰克福香肠的品质特性,且在添加量为0.3%时效果最佳。 相似文献
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ABSTRACT: This study investigated the use of sodium levulinate to prevent outgrowth of Listeria monocytogenes in refrigerated ready-to-eat (RTE) meat products. Turkey breast roll and bologna were formulated to contain 1%, 2%, or 3% (w/w) sodium levulinate, 2% sodium lactate, a 2% combination of sodium lactate and sodium diacetate (1.875% sodium lactate and 0.125% sodium diacetate), or no antimicrobial (control). Samples of the RTE products were sliced, inoculated with 102 to 103 CFU/cm2 of a 5-strain cocktail of L. monocytogenes , vacuum packaged, and stored at refrigeration temperature for 0 to 12 wk. Counts reached 108 CFU/cm2 on control turkey roll product after 8 wk, and over 107 CFU/cm2 on control bologna after 12 wk. Addition of 2% or more sodium levulinate to turkey roll and 1% or more sodium levulinate to bologna completely prevented growth of L. monocytogenes during 12 wk of refrigerated storage. A consumer taste panel with pathogen-free samples found no differences in the overall liking among the preparations of turkey roll or among preparations of bologna. These results show that sodium levulinate is at least as effective at inhibiting outgrowth of L. monocytogenes in RTE meat products as the current industry standards of lactate or lactate and diacetate, and levulinate addition does not alter the overall liking of the RTE meat products. 相似文献
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The effects of irradiation of refrigerated and frozen chicken on sensory properties were investigated on skinless boneless breasts (white) and leg quarters (dark). Irradiation did not affect appearance of moistness and glossiness of raw chicken (white or dark). Leg quarters irradiated while refrigerated were darker (p 0.05) than controls (nonirradiated chicken). Raw irradiated chicken had higher “fresh chickeny,” bloody, and sweet aromatic aroma intensities compared to nonirradiated samples. Cooked irradiated frozen dark meat had more chicken flavor, and cooked irradiated refrigerated dark meat was more tender than controls. No other sensory attributes of cooked chicken were affected. The state at which chicken had been irradiated (refrigerated or frozen) did not affect sensory properties. 相似文献
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Cheng-An Hwang;Lihan Huang;Shiowshuh Sheen; 《Journal of Food Safety》2024,44(6):e13175
Salmonella spp. and Staphylococcus aureus have been linked to foodborne illnesses caused by the consumption of processed meat products. This study examined the growth probabilities of these two pathogens as affected by sodium chloride (salt), sodium lactate, and sodium diacetate in a solid medium for using these three additives to improve the microbial safety of processed meat. Sterilized tryptic soy agar (TSA, 200 μL) formulated with a combination of salt (3%–8%, aw 0.98–0.93), lactate (0%–2.4%), and diacetate (0%–0.25%) and inoculated with Salmonella spp. or S. aureus was dispersed into 96-well microplates and incubated at 37°C for 7 days. After incubation, a well showing any Salmonella spp. or S. aureus colonies was denoted as a growth event, otherwise a no-growth event. The number of growth events for each formulation was recorded. The effects of the additives on the growth event were analyzed by logistic regression to identify the growth and no-growth boundaries and the formulations that may prevent the growth of Salmonella spp. or S. aureus. For Salmonella spp., the observed minimum no-growth concentrations in TSA were 3% salt with 0.8% lactate+0.2% diacetate or 1.6% lactate+0.1% diacetate, 4% salt with 2.4% lactate, 5% salt with 0.25% diacetate, 6% salt with 0.8% lactate+0.15% diacetate, 7% salt with 0.8% lactate or 0.15% diacetate, and 8% salt alone. For S. aureus, the concentrations were 3% salt with 2.4% lactate+0.2 diacetate, 5% salt with 1.6% lactate+0.2% diacetate, 7% salt with 0.8% lactate+0.25% diacetate, and 8% salt with 0.8% lactate+0.20% diacetate or 1.6% lactate+0.15% diacetate. These no-growth formulations also inhibited the growth of both pathogens in cooked meat samples. Mathematical models were developed to describe the effects of the additives on the growth probabilities of Salmonella spp. and S. aureus. Findings from this study may be used for formulating refrigerated and shelf-stable meat products to reduce Salmonella spp. and S. aureus risk. 相似文献
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Low-fat patties containing water, carrageenan, encapsulated salt and hydrolyzed vegetable protein (carrageenan-based patties) with 0, 1, 2 or 3% potassium lactate were compared to low-fat all-beef patties with no additives. Carrageenan-based patties had enhanced (P<0.05) sensory properties (juiciness, tenderness, mealiness and beef flavor intensity) compared to all-beef patties. The bacterial populations of low-fat, carrageenan-based patties did not differ (P<0.05) from low-fat all-beef patties. Bacterial growth in low-fat, carrageenan-based patties was reduced through the use of 2 or 3% potassium lactate with no deleterious effects on the sensory properties of the low-fat, ground beef. However, low-fat, carrageenan-based patties underwent greater (P<0.5) discoloration and lipid oxidation during aerobic refrigerated storage than all-beef patties. 相似文献