Accelerated evolution and molecular surface of venom phospholipase A2 enzymes

The objective of this study was to determine the in vitro shear bond strength (in megapascals) and location of bond failure with two light-cured glass ionomer resin systems. One system was a hybrid glass ionomer cement with resin (GC Orthodontics, Aslip, Ill), and the other system a glass-filled resin system (Reliance Orthodontic Products, Inc, Itasca, Ill). These systems, Fuji Ortho LC (GC Orthodontics) and Ultra Band Lok (Reliance), respectively, were compared to a light-cured composite resin. Maxillary premolar brackets (n = 200) were bonded to the facial surface of human premolar teeth. The two glass ionomer resin systems were each evaluated by two protocols, one according to the manufacturers' direction plus a variation of their respective technique. The five distinct groups (n = 40) were stored in 37 degreesC distilled water for 30 days and subjected to thermocycling before shear bond strength testing. The findings indicated that large variations existed between the bond strengths of the materials tested. The laboratory shear bond strength of the glass-filled resin glass ionomer cement (Reliance), whether tested in a dry or moist field, was similar to the composite control with all of the previous materials being significantly (P <.01) higher than both the hybrid glass ionomer cement groups (Fuji Ortho LC). However, the hybrid glass ionomer cement with enamel conditioner demonstrated a clinically acceptable mean megapascal value. The Adhesive Remnant Index values ranged from 0.53 to 1.62. The hybrid glass ionomer cement without enamel conditioning recorded the lowest mean adhesive remnant index score and the lowest mean megapascal score. Although both products are glass ionomer resin systems, their individual chemistries vary; this affects their clinical performance. Clinically, it may be suggested that glass ionomers used in a dry field may be beneficial for orthodontic bonding, and that glass ionomer resin systems used in a moist environment need an enamel conditioner.     

Clinical comparison between a glass ionomer cement and a composite for direct bonding of orthodontic brackets

The clinical performance of a glass ionomer cement for direct bonding of orthodontic brackets was compared with a composite resin routinely used in this procedure. Brackets were bonded, using both materials, in alternate quadrants of 16 patients of the Orthodontic Clinic of the State University of Rio de Janeiro. A total of 225 teeth, 112 in the glass ionomer cement group and 113 in the composite group, were tested. Bond failure frequencies were recorded for 12 months, and chi-square statistical test was carried out comparing the failure rates of the materials. The composite showed a statistically significant lower failure rate (7.96%) than the glass ionomer cement (50.89%), regardless of the dental arch tested. Although the glass ionomer cement presents important properties not observed in the composite, it is necessary to increase its cohesive strength to permit its clinical use for direct bonding of orthodontic brackets.     

Increased apoptotic cells in bone marrow biopsies from patients with aplastic anaemia

PURPOSE: To evaluate the 2-year clinical performance of two polyacid-modified resin composites and two resin-modified glass ionomers in Class V carious cavities. MATERIALS AND METHODS: A total of 120 Class V cavities were selected and 30 cavities were restored with one of two resin-modified glass ionomer materials (Fuji II LC Improved and Vitremer) and two polyacid-modified resin composites (Dyract and Compoglass) in Class V carious cavities after 2 years. The restorations were clinically evaluated after 1 and 2 years using the USPHS criteria. RESULTS: One-year findings revealed a significant difference in color match between Vitremer and other materials (P < 0.05) and no significant difference was found for the other criteria. Two-year results indicated a significant difference between resin-modified glass ionomers and polyacid-modified resin composite materials. The difference between Compoglass and Dyract was not statistically significant whereas the difference between Vitremer and Fuji II LC was statistically significant. Caries was not recorded at any evaluation period.     

The epidemiology of Neisseria gonorrhoeae isolates in Greenland 1979-1990: the emergence, spread and disappearance of non-PPNG strains carrying the conjugative 38.9 kb plasmid

The aim of this study was to compare the clinical performance of an amalgam, a glass polyalkenoate (ionomer) cerment material and a resin-based composite material used in small Class II cavities in permanent teeth. All restorations were inserted under rubber dam. They were examined yearly for 3 years. One clinician continued the study up to 5 years. The clinical examination focused on two criteria: clinically acceptable and failure. In addition, impressions were taken of the prepared cavities immediately before restoration and at each clinical examination using an elastomeric material. The study comprised 274 Class II restorations (88 amalgams, 95 cerments and 91 resin composites) placed in 142 adolescent patients. One hundred and sixty-seven restorations were in molar and 107 in premolar teeth. Patient dropout after 5 years resulted in the loss of 161 restorations, evenly distributed for restorative material and type of tooth involved. Four amalgam restorations, 22 glass ionomer cerment and nine resin composite restorations failed. The glass ionomer cerment and amalgam restorations failed primarily due to bulk fractures, while the resin composite restorations failed due to secondary caries and bulk fractures.     

Variability in cytotoxicity and fluoride release of resin-modified glass-ionomer cements

New-generation glass-ionomer cements contain resin to improve their restorative properties. These resin-modified glass-ionomer cements vary considerably in their chemistry, which could result in corresponding variability in their physical and biological properties. This study investigated the cytotoxicity and the fluoride release of two resin-modified glass ionomers, a conventional glass-ionomer cement, and a resin composite. Samples were prepared and extracted in distilled water for 1, 4, and 7 days; eluates were filtered and tested by means of 3T3 mouse fibroblasts. Cytotoxicity (MTT assay) values were low for all materials and extraction times, indicating minimal cytotoxicity of all materials (less than 30% inhibition). Cytotoxicity of one resin-modified glass ionomer was significantly higher than for the other materials (p < 0.001). One resin-modified glass ionomer and the conventional glass-ionomer cement released significantly more fluoride at each time interval (p < 0.001) than the other resin-modified glass-ionomer cement and the resin composite. Fluoride release and cytotoxicity were correlated (r2 = 0.60; p < 0.001), although the fluoride release does not account for the cytotoxicity observed. Cytotoxicity and fluoride release suggest that one hybrid behaved more like a conventional glass ionomer, and the other like a resin composite. These differences may have implications for material selection in specific clinical situations.     

Effect of dynamic loading methods on cement film thickness in vitro

PURPOSE: Reduced cement flm thicknesses can improve crown seating and decrease marginal discrepancies. Improved marginal adaptation has the potential to reduce plaque accumulation, periodontal disease, and cement dissolution. Studies have indicated that dynamic seating methods can reduce seating discrepancies associated with zinc phosphate and resin cements. However, other types of cements and other dynamic techniques have not yet been studied or compared, nor has the mechanism for improved seating been fully explained. Therefore, the purpose of this study was to investigate the effect of a variety of loading methods on the film thicknesses of current types of crown and fixed partial denture cements compressed between glass plates. MATERIALS AND METHODS: This study investigated the effect of three dynamic loading methods on film thickness of six representative fixed prosthodontic cements. These included zinc phosphate, resin-modified glass ionomer, encapsulated glass ionomer, adhesive composite resin, composite resin, and polycarboxylate. The method was derived from American Dental Association specifications for cement film thickness. In control groups, the cements were placed between two glass glass plates and statically loaded with a 15-kg weight. The test groups were initially similarly loaded, and then for 30 seconds further subjected to simulated repeated patient opening and closing, vibrations from an electromallet, or an ultrasonic device. RESULTS: Mean film thicknesses ranged from 7.4 micrometers for polycarboxylate / ultrasound up to 40.3 micrometers for composite resin / static. Two-way analysis of variance revealed that the effects of material type and cementation method and their interaction all significantly affected film thickness (P < .0001). Multiple range analysis showed that dynamic methods were generally superior to static loading and that the ultrasonic method was the best overall. CONCLUSIONS: The different dynamic loading methods all significantly decreased cement film thicknesses between glass plates. The ultrasonic method was the most effective. The type of cement used also influenced film thickness. Composite resins were more affected than other materials.     

The effect of a resin-modified glass ionomer restorative material on artificially demineralised dentine caries in vitro

OBJECTIVES: Recurrent caries is one of the most common reasons for the replacement of restorations. One method of reducing the frequency of this problem may be by using fluoride-releasing restorative materials. The aim of this in vitro study was to evaluate the progression of artificial lesions around resin-modified glass ionomer (Vitremer) restorations placed in root surfaces. Class V cavities were prepared on the mesial and distal surfaces of 28 human premolar teeth. Artificial carious wall lesions were created in all cavities. The root of each tooth was then hemisected through the middle of the two cavities, before being restored with either the glass ionomer or amalgam, while the opposing cavities on the same root portion were varnished as negative controls. Forty-eight specimens were pH-cycled in remineralising and demineralising solutions for 20 h and 4 h, respectively, each day for 4 weeks, whilst in a smaller control group of eight specimens, the demineralising solution was replaced with deionised water. Mineral changes in the carious lesions were evaluated using contact microradiography. RESULTS: Results showed that varying degrees of subsurface demineralisation and remineralisation were evident, with a laminated appearance in lesions adjacent to the glass ionomer. There was higher remineralisation in the glass ionomer-filled cavities compared with the amalgam-filled cavities. In the water-cycled group, glass ionomer showed an increase in mineral content (p < 0.05), while no changes were observed in the amalgam-filled cavities. CONCLUSIONS: This model has shown that glass ionomer has a greater potential than amalgam for remineralisation of artificially created wall lesions within an acidic environment.     

The effect of adhesive luting agent-dentinal surface interactions on compressive strength of two types of all-ceramic crowns

This research compared the compressive strength of two types of all-ceramic crown (Hi-Ceram and Duceram) as affected by selected luting cements (Zn phosphate, glass ionomer and composite resin cement). Thirty crowns of similar size and shape were constructed (15 crowns of each tested material) to fit a standard posterior tooth preparation. Five crowns from each material were cemented by one of the tested cements. The cemented crowns were loaded until catastrophic failure. A two-way analysis of variance was performed and showed that the type of utilized cement had a significant effect on the compressive strength being that Panevia Ex. resin cement the most effective one followed by glass ionomer and then finally zn phosphate cement. Statistical analysis also showed that Hi-Ceram crowns were more resistant to occlusal load than Duceram.     

A procedure for preparing undecalcified and unembedded bone sections for light microscopy

Recently, new restoratives, such as resin-modified glass ionomer cements (RMGIC) and polyacid-modified resin composites (PMC) were introduced for class III and class V cavities. Both materials use simplified cavity conditioning methods. The well-established treatment of enamel with phosphoric acid has been replaced with treatment using weaker acids. The purpose of this study was to investigate in vivo the quality and durability of the marginal bond to enamel of these restorative system and compare it with a resin composite restorative, Seventeen patients received class III restorations of each of the three restoratives. At baseline and after 1 year replica impressions were made for investigation of the vestibular margins with the scanning electron microscope. Semi-quantitative analysis of the enamel-restorative interfaces was performed at x200 and x1000 magnifications. The three restorative systems showed good marginal adaptation and high percentages of the length of the margins investigated at baseline were gap-free (82%-92%). The resin composite showed significantly better adaptation than the other materials. The marginal quality decreased significantly after 1 year for the resin composite and the polyacid-modified resin composite. The RMGIC showed improved sealing after 1 year in vivo, probably due to continuing water uptake. The percentages of gap-free margins of the total marginal length observed at 1 year were 73%, 90%, and 84%, respectively, for the PMC, the RMGIC and the resin composite. The difference between the PMC and the RMGIC was significant. In conclusion, a good marginal quality was seen for all three restorative systems in class III cavities after a period of 1 year.     

Resin-modified glass ionomers for luting posterior ceramic restorations

OBJECTIVES: Until recently, esthetic inlay restorations in posterior teeth have been limited to cavities surrounded by enamel. Dentin adhesive systems in combination with luting composites and light-cured resin-modified glass ionomer cements offer a possibility for bonding ceramic inlays to cavities when the cervical margin is in dentin. This study was designed to compare in vitro marginal integrity of ceramic inlays bonded to dentin to restorations placed in cavities with margins located entirely in the enamel. METHODS: In the present in vitro study, the sealing abilities of a dentin bonding agent/luting composite combination (Syntac/Dual Cement, Vivadent) and resin-modified glass ionomers (Photac Fil, Photac Bond, ESPE; Dyract, De Trey Dentsply; Fuji II LC, GC Dental Industrial Corp.; and Vitremer, 3M Dental Products) used as luting agents in cavities extending beyond the cemento-enamel junction, were compared to the sealing abilities of a conventional luting composite (Vita Cerec Duo Cement, Vita) in cavities within sound enamel. SEM analysis and dye penetration were performed to evaluate marginal integrity at the cervical cavity margins. RESULTS: The dentin bonding agent/luting composite combination (Syntac/Dual Cement) rendered a marginal seal within the dentin similar to the quality obtained with the conventional luting procedures within sound enamel. When three out of the five resin-modified glass ionomers were used as luting agents (Dyract, Fuji II LC and Vitremer), the results were comparable to those reported for the dentin bonding agents and the conventional method. SIGNIFICANCE: Light-cured resin-modified glass ionomer cements may be considered as an alternative to dentin bonding agents when the cavity margins of ceramic inlay restorations are within the dentin. However, further studies, e.g., wear resistance, must be performed.     

Fracture resistance in conservative Class II cavity preparation: box vs tunnel

A total of 40 extracted human maxillary premolar teeth were used in this study. Teeth were randomly distributed between two main groups. In group A conservative class II Box preparations were cut in each tooth. In group B the proximal cavity was prepared through the occlusal surface and the proximal marginal ridge was left intact (tunnel preparation). Group A and group B were randomly subdivided into two subgroups (a & b). In groups Aa and Bb a posterior composite resin material was used to restore the prepared cavities (no base was used). While in groups Aa & Bb the posterior composite resin was used with a glass ionomer cement base. The teeth were tested on an Instron testing machine for their resistance to fracture, force being applied equally to buccal and lingual cusps. Mean fractures values were: Gp. Aa 285 LB.: Gr. Bb 245 lb. Bb 240 lb. One way analysis of variance showed no statistical difference between the four groups. The tunnel preparation did not increase the resistance to fracture of the restored tooth compared to a box preparation.     

Resin modified glass ionomer cements. In vitro microleakage in direct class V and class II sandwich restorations

Microleakage of conventional chemically cured and resin modified glass ionomer cements (GIC), a compomer and a composite resin was studied in vitro in direct class V and class II GIC/composite sandwich fillings. None of the restorative techniques investigated completely resisted microleakage at both the occlusal and gingival margins. The dentinal gingival margins exhibited the highest leakage pattern. The compomer and the resin modified GIC showed a better seal than the chemically cured GIC's. The use of a liner as separating agent between composite resin and GIC in the class II sandwich fillings did not improve the seal. The results suggest that the initial bond obtained immediately after light curing of the resin modified GIC's is stronger than that for chemically cured GIC's. The contradictory results of dye leakage studies reported in the literature are discussed and in vivo evaluations are suggested necessary to predict clinical performance.     

Bond strengths of fluoride-releasing restorative materials

PURPOSE: To compare the shear bond strengths to dentin of four tooth-colored fluoride-releasing restorative materials that utilize different mechanisms of dentin-bonding. MATERIALS AND METHODS: Four materials were tested: a chemically-cured glass ionomer (Ketac-Fil); a light-cured resin-modified glass ionomer (Photac-Fil); a light-cured resin-modified glass ionomer in conjunction with a resin dentin-bonding agent (Vitremer); and a light-cured resin composite in conjunction with a resin dentin-bonding agent (Tetric). The enamel was removed from four sides of the twenty human noncarious extracted molars which had their roots embedded in acrylic blocks. Cylindrical samples of the materials were prepared in plastic molds and bonded to the dentin surface according to the manufacturers' instructions. After 24 hours of storage in a humidor, the samples were sheared with an Instron testing machine at a crosshead speed of 0.5 mm/minute. RESULTS: Statistical evaluation (ANOVA and Duncan's test) suggests that the fluoride-releasing resin composite material utilizing a resin dentin-bonding agent provided a significantly greater mean shear bond strength (16.5 MPa) after 24 hours storage than any of the selected glass ionomer materials. Of the three glass ionomer materials compared, the light-cured resin-modified material with a resin dentin-bonding agent provided a significantly greater mean shear bond strength (6.7 MPa) than the light-cured resin-modified material without a resin dentin-bonding agent (3.0 MPa) or the chemically-cured material (3.8 MPa).     

An in-vitro investigation of microleakage and gap size of glass ionomer/composite resin "sandwich" restorations in primary teeth

Eighty extracted primary molars were divided into four groups of twenty teeth. Class II cavities were prepared in all teeth with equal numbers of proximal boxes having cavosurface margins either in enamel, or dentin/cementum. Each group was allocated to an open or closed sandwich technique using glass ionomer as a lining. Following the placement of the composite resin restorations, the gap size measured at the proximal box was greatest for the closed sandwich group with the cavosurface margin on enamel (0.203 microns) or dentin/cementum (0.174 microns). Microleakage scores were measured at the proximal box and were greatest for the closed sandwich group with the cavosurface margin on dentin/cementum. The best result was obtained for the open sandwich group with the cavosurface margin on enamel.     

Surface structural change of bioactive inorganic filler-resin composite cement in simulated body fluid: effect of resin

Recently much attention has been paid to bioactive filler-resin composite cements because they can solidify in a few minutes to give high mechanical strengths and they can bond to living bone. In this study the dependence on resin of apatite-forming ability in simulated body fluid (SBF) was investigated for the composite cements of bioactive CaO-SiO2-P2O5-CaF2 glass with polymethyl methacrylate (PMMA) or bisphenol-a-glycidyl methacrylate/triethyleneglycol (Bis-GMA/TEGDMA) resin. The PMMA-containing composite cement did not show the apatite-forming ability in SBF because the reaction of the glass grains with SBF was inhibited due to the complete covering of the grains with PMMA. To the contrary, the Bis-GMA/TEGDMA-containing cement exhibited high apatite-forming ability in SBF; these monomers significantly dissolved from the composite surface into SBF, causing a direct exposure of the glass grains to SBF to convert into silica gel. It is assumed that thus formed silica gels, and the silicate ions that were dissolved and adsorbed onto the composite surface, induced the apatite nucleation between the spaces of the glass grains and on the composite surface, respectively. A continuous bone-like apatite layer was formed on the top surface of the glass-Bis-GMA/TEGDMA composite cement in a short period.     

Initial tensile bond strength of resin-modified glass ionomers and polyacid-modified resins on perfused primary dentin

The objective of this study was to evaluate the initial tensile bond strength of a resin-modified glass ionomer (Photac-Fil) and two polyacid-modified composite resins (Compoglass, Dyract) to primary dentin. A hybrid composite resin (Tetric) and two chemical cured glass ionomers (BaseLine, Hi-Dense) served as controls. Ninety caries-free dentinal discs were ground flat and perfused with Ringer's solution. Dentinal surfaces were conditioned (except for Base Line). From each material, fifteen standardized specimens were attached to the dentin. Light-curing of the respective materials followed (1 min), and adhesion was tested with a universal testing machine 15 min after application. The highest initial bond strength was observed with Tetric (5.17 MPa). Closed test procedure (Kruskal-Wallis) showed significant differences between all materials (P < 0.05), except for Compoglass (1.82 MPa) vs. Dyract (2.35 MPa), and BaseLine (0.37 MPa) vs. Photac-Fil (0.42 MPa). The condensable glass ionomer Hi-Dense revealed a mean tensile bond strength of 0.79 MPa. Adhesion of (polyacid-modified) composite resins is superior to the other tested glass ionomer materials, when applied to perfused primary dentin.     

Marginal adaptation of resin-bonded light-cured glass ionomers in dentin cavities

PURPOSE: To investigate the marginal adaptation of resin-modified glass ionomer cements in dentin cavities placed with or without additional application of resin bonding systems. MATERIALS AND METHODS: Three resin-modified materials (Fuji II LC, Photac-Fil, Vitremer), one compomer (Dyract) and as reference an adhesively bonded resin composite system (Gluma CPS-Pekafill) were used. Flat peripheral dentin surfaces on human molar teeth were produced by wet grinding on SiC paper. Cylindrical cavities, 3.5 mm wide, were prepared in these dentin areas and restored with the individual materials. Sixty cavities were pretreated and restored as requested by the respective manufacturers. Following water storage of the specimens for 15 minutes or 24 hours, excess was gently removed by wet grinding for microscopic inspection of the marginal area. Additionally, in 30 cavities an experimental one-component adhesive resin system, a proprietary dimethacrylate and HEMA mixture dissolved in acetone, was combined with each of the restoratives for evaluation after 15-minutes water storage. Finally, in six cavities each, Dyract was combined with Prime and Bond 2.0, and Vitremer with Scotchbond Multi-Purpose Plus for assessment after 15 minutes. Maximum marginal gap widths (MGW) were measured. One-way ANOVA by ranks (Kruskal-Wallis-Test) followed by Wilcoxon's Two-Sample test were used to study the statistical difference of MGW among the treatment groups at a rejection level P = 0.05. RESULTS: Neither the conventionally placed material systems nor the restorations in combination with adhesives showed consistently gap-free margins after 15-minute water storage. After 24-hour storage with Vitremer 4 of the 6 restorations were gap-free, whereas with all other materials only perfect margins were registered. There was, however, no significant difference between the groups. Application of the experimental and/or the specific resin bonding agents had no effect on early MGW except for the Photac-Fil group, which was significantly reduced.     

Psychiatric comorbidity among hospitalized AIDS patients vs. non-AIDS patients referred for psychiatric consultation

This study evaluated the clinical performance of a new resin modified glass ionomer cement, Geristore (Den-Mat Corp., Santa Maria, Calif.), for the bonding of orthodontic brackets and its effect on certain caries-associated microorganisms. This cement has been shown to possess increased mechanical properties and long-term fluoride release. There were 716 brackets bonded in 40 patients (17 males and 23 females), with a split-mouth technique and a composite resin, Phase II (Reliance, Itasca, III.), as a control. Bond failures were recorded up to 1 year. Plaque scores and plaque samples were taken from the area of the bonding adhesive in 20 patients, before, at 1 week, and 5 months after the placement of brackets. The plaque samples were investigated for the presence of Streptococcus mutans and lactobacilli. The overall bond failure rate was found to be 8.9% for Geristore and 3.1% for Phase II (p < 0.05). Labially, there was no significant difference (p > 0.05) in bond failure rate: 3.8% for Geristore and 1.7% for Phase II. The proportions of S. mutans and lactobacilli in plaque taken from around Geristore cement were reduced at 1 week and 5 months, when compared with Phase II resin, and this reduction was statistically significant (p < 0.05) at 1 week. Results of this study suggest that Geristore may be of use in the labial segments, especially in caries prone patients, in whom demineralization at debond may present an esthetic and restorative problem several years after treatment.     

Uptake and release of fluoride by saliva-coated glass ionomer cement

The 'recharging' of aged glass ionomer cement restorations with fluoride by exposing them to a concentrated NaF solution has been suggested to improve their performance as devices with slow fluoride release. We have studied the interference of salivary coatings on glass ionomer cement with the uptake of fluoride in vitro. Freshly prepared glass ionomer discs were leached in distilled water for 14 weeks, then incubated in human saliva for 2 h, 24 h or 1 week before exposure to 0.53 M NaF (1% fluoride) for 5 min. Fluoride uptake by the cement was measured indirectly as the subsequent increase in fluoride release. A 2-hour incubation in saliva reduced the fluoride uptake by half, a 24-hour incubation by 74%. The major part (93-95%) of the fluoride that was taken up was released on the first day. A 1-week salivary coating reduced the uptake by 49%, but also caused some retardation of the fluoride release: 80% of the total release was on the first day. It was concluded that despite the considerable interference by salivary coating, the amount of fluoride that can be taken up by aged glass ionomer cement remains significant. However, the fast release will limit the usefulness of such recharging in vivo.     

Effect of peroxide bleaches on fluoride release of dental materials

PURPOSE: To investigate the effect of two bleaching agents, 35% hydrogen peroxide (Superoxal) and 10% carbamide peroxide (Opalescence), on the release of fluoride from Ketac-Fil, a glass ionomer cement, Photac-Fil, a glass ionomer/resin hybrid; and Tetric, a resin composite with fluoride. MATERIALS AND METHODS: Seven disks (9 x 3 mm) of each material were immersed in Superoxal for 1 hour to simulate an in-office application; seven other disks of each material were immersed in Opalescence 1 hour daily for 7 days to simulate a week of home bleaching regimen; and seven additional disks of each material without bleach served as a control. The disks were kept in deionized water and the water was tested for fluoride released every 24 hours for 14, 21, 28, and 35 days, respectively. RESULTS: Repeated measures ANOVA showed that the mean daily release of fluoride did not differ significantly (P > 0.05) between the bleaching agents or the control: Superoxal 6.17 ppm, Opalescence 6.45 ppm, and control 6.50 ppm. Statistically significant differences (P < 0.05) occurred for mean daily fluoride (+/- standard error) released from the three materials over the 35-day period: Photac-Fil 12.31 +/- 0.48 ppm > Ketac-Fil 7.07 +/- 0.66 ppm > Tetric 0.12 +/- 0.01 ppm, respectively.