羟基磷灰石/胶原-魔芋葡甘聚糖多孔支架研究

采用共滴定法制备了羟基磷灰石／胶原复合粉体,通过凝胶-冷冻干燥法制备了羟基磷灰石／胶原-魔芋葡甘聚糖多孔支架。分析了碱加入量对支架显气孔率、孔径及强度的影响。XRD与TEM结果显示复合粉体中无机相为纳米羟基磷灰石。所制备的支架显气孔率在80％以上,抗压强度在0.45—1.31MPa,SEM结果显示孔径分布在100—300μm。细胞相容性实验证明表明细胞在支架上生长良好。     

低聚KGM醛酸丙酯硫酸酯钠盐的结构分析

利用傅立叶红外光谱、激光拉曼光谱、X衍射光电子能谱、核磁共振波谱等现代仪器分析手段对低聚葡甘聚糖醛酸丙酯硫酸酯钠盐的结构进行分析.结果表明低聚葡甘聚糖经丙酯化修饰和硫酸酯化后,糖环的C2和C3位的羟基上和C6位连接的羟丙基上的羟基接有硫酸基团,且硫元素的相对含量为7.94%.     

魔芋葡甘聚糖硫酸酯的制备及其体外抗凝血活性研究

以魔芋精粉为原料,在N,N-二甲基甲酰胺(DMF)介质中通过磺化反应成功制备出魔芋葡甘聚糖硫酸酯;以元素分析、红外光谱及激光光散射-尺寸排除色谱联用仪研究了其结构,并用体外凝血试验测定了目标化合物活化部分的凝血活酶时间APTT(Activatedpartialthrombintime)、凝血酶时间TT(Thrombintime)和凝血酶原时间PT(Prothrombintime),同时,探讨了魔芋葡甘聚糖硫酸酯的取代度DS(Degreeofsubstitution)与抗凝血活性的关系。     

透明质酸钠/魔芋葡甘聚糖软骨支架材料的研究

采用共混法制备了透明质酸钠（SH）/魔芋葡甘聚糖（KGM）软骨支架材料,通过正交实验,根据抗压强度、吸水率、孔径分布和空隙率等对支架进行了制备条件（SH、KGM、氨水含量和冷冻温度）的优选,通过光学显微观察、XRD、IR、体外模拟降解、兔骨髓间充质干细胞共培养和SEM观察,初步检测了软骨支架的材料结构和生物性能,证明材料有较好的干细胞相容性,无短期急性毒性,可以进一步进行开发研究.     

骨组织工程用三维支架制备的研究现状

骨组织工程支架材料是骨组织工程研究的关键问题之一。国内外骨组织工程多孔支架的制备主要有溶液浇铸/粒子沥滤法、热致相分离/冻干法、发泡法、激光烧结法等多种方法,但所制备的复合材料强度等性能仍不理想。提高机械性能、大孔径高孔隙率和可塑成型性,并使其具备细胞识别信号将是支架材料研究的热点和难点。     

一种改性羟基磷灰石/聚乳酸的多孔支架

通过将热致相分离与溶致相分离技术结合,制备了一种新型具有双连续孔洞的聚(γ-苄基-L-谷氨酸)/聚乳酸多孔支架材料。体外生物活性实验表明该支架材料能够很好的促进类骨磷灰石的形成,表现出优良的生物活性,表明这类支架材料在骨组织工程领域具有很好的应用前景。     

壳聚糖基骨组织工程支架材料的研究进展

壳聚糖是甲壳素脱乙酰基的产物,是第二大天然高分子,由于其良好的生物相容性、生物可降解性和成型性,作为骨组织工程支架材料越来越受到重视.对壳聚糖为主的复合骨组织工程支架的相关研究进行综述,可以掌握其在不同的材料体系中的功能作用,并可预见壳聚糖基生物材料在骨组织工程中的发展潜力.     

脂酰葡甘聚糖醛酸钠-工程蝇抗菌肽复合性能研究

介绍了将脂酰葡甘聚糖醛酸钠添加到工程蝇抗菌肽中的试验研究,试验证明,脂酰葡甘聚糖醛酸钠 可改善其组织结构,提高其水溶性、稳定性,转化产品的乳化效率、膨胀率和保型效果,并获得添加脂酰葡甘聚 糖醛酸钠的最佳条件是：酯酰葡甘聚糖醛酸钠用是为１２％,水用量为６５％,温度为７０℃。     

壳聚糖与葡甘聚糖共混材料的制备及其性能表征

以葡甘聚糖和壳聚糖为主要原料,采用36％的乙酸溶解壳聚糖,通过共混的方法制备壳聚糖与葡甘聚糖共混膜液,经静置减压脱泡、倒膜、干燥制膜,并用与乙酸等物质量的氢氧化钠对膜进行处理。通过IR、XRD和生物学显微镜对共混膜进行性能表征,同时测定了共混膜的透光率、力学性能、吸水率及水蒸气透过系数。结果表明：共混膜的力学性能和水蒸气透过系数得到改善,葡甘聚糖和壳聚糖之间有强烈的相互作用和良好的相容性,作为一种潜在的皮肤组织工程支架将具有良好的应用前景。     

魔芋葡甘聚糖超声波辅助纯化工艺研究

采用超声波辅助技术,以乙醇为纯化溶剂,优化魔芋葡甘聚糖的纯化工艺,分别对乙醇浓度、超声温度、料液比、超声功率、超声时间进行单因素和正交试验,并通过极差分析对纯化过程显著影响葡甘聚糖含量的因素进行统计分析．结果表明,超声波辅助纯化魔芋葡甘聚糖的最佳工艺条件为：超声温度55℃,乙醇浓度25％,料液比1：180,超声功率190W,超声时间30min,该工艺条件下魔芋葡甘聚糖的含量可达97．34％,该纯化工艺效率高、作用时间短且结果稳定,可靠．     

Composition and Structure of Fibrous Hydroxyapatite Growth on an Injectale Bone Tissue Engineering Scaffolds Material

1Introduction Thetreatmentofbonedefectsinducedbyinjuryor pathologicalchangecouldbenefitfrombiocompatiblema terialsbasedonbiodegradablepolymerthatassistinthe boneregeneration.Thesepolymersareverypromisingasa temporarystabilizationforaugmentationoffracturef…     

Composition and Structure of Fibrous Hydroxyapatite Growth on an Injectale Bone Tissue Engineering Scaffolds Material

Fibrous hydroxyapatite （ HA ） wns grown upwards from the crosslinked unsaturated polyphosphoester（ UPPE ） which was used us an injectable bone tissue engineering scaffolds. Composition of fibrous HA was determined by FT- IR, XRD and EDX, which suggested that the fibrous HA was calcium deficient carbonated apatitie with low crystallinity. SEM micrographs indicated that the fibrous HA had a hollow tubing structure and tube wall wus a flakelike assembly. The fibre with poor mechanical property arm with a growth rate about 0. 5 mm/min reached several centimeters in length after 2 hours. The growth was at the tip of the fibre suggested that the procedure of forming fibrous HA was as follows ： Co^2＋ ions were firstly incorporated into the crosslinked UPPE by dipping in Ca^2＋ solution, then supplied through micropores of the material reacted with PO4^3- ions to form α small tuhe , the osmotic pressure or capillary force lead the Ca^2＋ continuously gushed oat into the PO4^3- solution, thus fibrous HA was obtained.     

静电纺纳米纤维支架在组织工程中的应用进展

综述了采用静电纺丝制备的纳米级纤维在人工皮肤、骨组织引导、神经修复、血管移植和心肌与骨骼肌再生等组织工程领域中作为支架材料的应用进展.     

Biologically Inspired Self-assembling Synthesis of Bone-like Nano-hydroxyapatite/PLGA-(PEG-ASP)_n Composite: A New Biomimetic Bone Tissue Engineering Scaffold Material

1Introduction Replacementofbonetissuebygraftmaterialsand productsoftissueengineeringhavingcomposition,struc ture,andbiologicalfeaturesthatmimicnaturaltissueisa goaltobepursued[1].Poly(lacticacid)(PLA),poly(glycolicacid)(PGA),andtheircopolymers(PLGA)havebe…     

皮肤组织工程支架材料与制备方法的研究进展

皮肤组织工程支架材料在人工皮肤的构建中起着关键作用,为种子细胞提供了黏附、生长、迁移、增殖、分化和代谢的环境,是皮肤组织工程的重要研究内容。对组织工程化皮肤支架近几年的材料、制备方法的进展进行了综述,并分析了皮肤组织工程支架材料的发展趋势。     

Design and Fabrication of Manual Bone Scaffolds via Rapid Prototyping

1 Introduction Porous ceramic scaffolds can be produced using various methods ,including conversion from natural struc-tures and completely synthetic techniques[1 ,2]. Conversion methods start with a naturally occurring structure ,such a coral1 or trabecular bone . They are converted to the final composition while maintaining the original ar-chitecture[3].Completely synthetic techniques employ several very different methods for creating pores . Thesemethodsinclude the foaming of ceramic slurr…     

Synthesis and Characterization of a Novel pH-sensitive Complex for Drug Release

A novel pH-sensitive complex was prepared by using oxidized konjac glucomannan and 4-aminosalicylic acid (4-ASA) through glutaraldehyde as a cross-linking agent. The product was characterized by FTIR and 13C NMR spectra, and the thermogravimetric analysis was also studied. The drug release studies in vitro showed that the amount of 4-ASA released from the complex was about 4%, 56% and 17% after 12 h at pH 1.2, 6.8 and 7.4, respectively. The data demonstrate that the rate of the drug release of the complex c...     

基于MATLAB的骨支架转变成骨组织的仿真

把计算机技术运用于骨移植材料转变为骨组织的复杂行为研究中,采用元胞自动机方法建立仿真的计算模型,以MATLAB为开发工具,实现了模型的并行仿真并再现了仿真的动态过程,为计算机辅助骨组织工程提供了新的研究方法和工具.