凝胶色谱电泳分离装置及过程理论的研究

研制成功凝胶色谱电泳(CACE)分离装置,采用标准蛋白质模式混合物进行实验研究。通过理论分析,用体积平均方法建立了过程数学模型,计算并比较了不同条件下目标蛋白质的聚焦过程及分离操作的动态特性,说明了流场与电场强度对分离过程的重要影响。模拟结果与实验数据充分吻合。该模型对CACE分离过程及装置的设计和优化提供了理论依据。     

连续型逆向色谱电泳技术研究

介绍了连续型逆向色谱电泳分离技术的原理及其研究进展，现状和存在的问题，提出了需进一步研究和探讨的方向。     

羟基磷灰石电色谱中电渗流动与分离过程特性

在羟基磷灰石色谱上施加电场 ,发展了一种新的电色谱分离技术 .对蛋白质电泳和溶液电渗对分离特性的影响进行了实验研究 ,测量了羟基磷灰石电色谱装置中膜、膜与填充介质在不同浓度磷酸缓冲溶液和在不同电流下产生的电渗流 ,分别计算出由膜和羟基磷灰石电渗而产生的推动力 .实验结果表明电渗流量随缓冲溶液离子强度的降低和电流的增加而增加 .在施加电场的情况下 ,羟基磷灰石对牛血清白蛋白的吸附速度加快 ,动态吸附量增加 .分别考察了不同洗脱条件下电场对洗脱效果的影响 .在高浓度的洗脱溶液下 ,电渗的作用很小 ,电场的作用主要是电迁移的作用 ,可以通过选择适宜的电场方向来提高分离精度 ;在低浓度的洗脱溶液下 ,电渗的作用有效地促进了洗脱液与介质颗粒的传质 ,提高了洗脱效果 .在羟基磷灰石色谱上施加电场提高了该色谱分离过程的速率和效率 .     

毛细管电泳在蛋白质分离分析中的应用

简单介绍了毛细管电泳主其在蛋白质分离分析中的应用。     

连续制备色谱的研究进展

本文介绍了近30年来连续制备色谱分离的研究概况，从逆流和错流两个方面分别表述了半连续色谱精制器和连续环状色谱的基本原理，研究进展及应用前景。     

连续环状离子交换色谱分离技术

本文介绍了一种新型制备色谱分离技术──连续环状离子交换色谱ＣＡＣ，建立了二维稳定连续环状离子交换色谱的数学模型。在一定条件下经过适当转化，二维稳态ＣＡＣ过程可转化为一维非稳定色谱过程。分别改变ＣＡＣ进料浓度、进料流速及冲洗流速等操作条件，测定了ＣＡＣ对果葡糖的分离性能。同时，用固定床线性色谱理论对ＣＡＣ进行预测，其预测值与实验结果较好地相吻合。实验得出，连续环状离子交换色谱对果葡糖具有较好的分离性能。     

制备型电动亲和色谱吸附过程传质动力学

建立了描述电动亲和色谱分离过程吸附操作的传质方程 ,研究了人血清白蛋白 (HSA)和BlueSepharoseFastFlow (Blue)体系中Blue介质的电渗特性及其影响因素 ;测定了Blue对HSA的吸附等温线以及在不同交变电场下的动态吸附曲线 ;由模型计算得到了吸附操作的传质系数及其与电场强度和交变频率的关系 ,实验结果和计算结果均表明 ,施加交变电场有助于提高传质系数 ,从而强化吸附过程的传质 .实验结果揭示了电渗流在强化色谱介质颗粒内传质的重要作用及其在发展大规模电动色谱技术中的良好应用前景     

连续床色谱分离技术

连续床色谱是一种新型分离技术,连续床与传统柱色谱相比,最大的优势在于它的孔道壁不允许蛋白质和多肽通过,这就需要它的分辨率不随流速变化而变化,或者随流速增加,分辨率增大,这样避免了传统层析柱中分辨率与流速二者不可兼顾的难题,并且其制备简单,稳定性好,本文介绍了连续床色谱的概念,制备及其优点,分析了连续床色谱目前存在的问题,并展望了其应用前景。     

生物分离技术与过程研究进展

简要介绍了生物分离技术的最新研究进展,重点讨论了这些新技术的创新之处及其应用前景。特别介绍了生物分离过程特性研究进展。应该指出的是,该方面的研究值得学术界和工业界重视。     

丙烯腈连续水相沉淀聚合过程的在线研究

在模拟工业流程的连续小试装置上进行了丙烯腈和醋酸乙烯酯的水相沉淀共聚合在线研究.考察了连续小试装置的操作特征等.通过聚合体系pH值和聚合温度实时反馈控制技术,即时分析聚合过程特征,发现体系pH值是最敏感的操作参数,而且响应灵敏和精确,能及时提供一些非稳定聚合状态的信息.     

STUDIES ON COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS AND ITS UTILIZATION FOR PROTEIN PURIFICATION

A counteracting chromatographic electrophoresis(CACE)separation device has been success-fully built and tested.Experiments are performed with protein mixtures of BSA,Hg,Mg and Cyto-cto study the separation performances,concentration profiles,start-up methodology as well as influ-ences of pH and ionic strength on the separation process.Mathematical models based on the fluiddynamics of the porous medium are proposed.Experiments indicate that the CACE process has agood commercial potential in protein engineering and fine chemicals purification.     

生化反应──色谱分离耦合过程模型研究

建立了描述生化反应─色谱分离耦合过程的一般数学模型,以蔗糖酶促水解制果糖、葡萄糖为模型反应,研究了底物转化率和产物分离度与操作条件的关系.理论计算值与实验结果在低浓度下符合较好．     

Nonlinear modeling of protein separation in a preparative‐scale dynamic field gradient focusing instrument

Dynamic field gradient focusing (DFGF) uses an electric field gradient opposed by a counter‐flow of buffer to separate milligrams of proteins according to their electrophoretic mobilities. A nonlinear model of protein separation in a preparative‐scale DFGF device was developed to aid in refining the instrument's design and finding optimal run conditions prior to performing experiments. The model predicted the focal points of bovine serum albumin (BSA), and bovine hemoglobin (Hb) to within the 95% confidence intervals about the means of the experimental values. The resolution between the proteins in the model was 2.08, which was 3% less than the lower limit of the 95% confidence interval about the experimental value. The model predicted 67% more dispersion than was present in the experimental device, which made the simulated BSA peak 22% wider than the experimentally measured width. © 2008 American Institute of Chemical Engineers AIChE J, 2009     

螺旋藻藻蓝蛋白的分离纯化研究进展

藻蓝蛋白在医药、食品及化妆品等领域有着广泛应用,螺旋藻中藻蓝蛋白的含量丰富。本文介绍了藻蓝蛋白的结构和生理活性,综述了国内外螺旋藻中藻蓝蛋白的提取和纯化技术的研究进展,列举了几个影响藻蓝蛋白稳定性的因素。     

Uniformly sized molecularly imprinted polymers for on‐line concentration,purification, and measurement of nimodipine in plasma

Uniformly sized molecularly imprinted polymers (MIPs) for nimodipine have been prepared in an aqueous system by multi‐step swelling and polymerization method, utilizing 4‐vinylpyridine (4‐VPY) or methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a cross‐linking agent. Scanning electron microscopy was used to identify the structure features of the obtained polymers. Further, the influences of some chromatographic conditions were examined to explore the possible recognition mechanism. The results reveal that stable molecularly imprinted polymeric microspheres with good size monodispersity were obtained, and the polymer beads showed specific recognition for the template molecule and some other dihydropyridine calcium antagonists (DHPs). Besides hydrophobic interaction, the molecular shape complementation of DHPs and the MIPs seems to play an important role in the retention and recognition of DHPs. The Scatchard analysis showed that two kinds of binding sites existed in the MIPs. The MIPs was then used as a high‐performance liquid chromatography (HPLC) separation medium to simultaneously concentrate and purify nimodipine in plasma. The results reveal that the obtained MIPs could be used for on‐line concentration, purification, and measurement of nimodipine in biological samples. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

助剂和制备方法对V2O5/Al2O3催化性能的影响

用浸渍法制备了负载型MxOy-V2O5/Al2O3催化剂,同时考察了不同的制备方法对MoO3-V2O5/Al2O3催化剂的性能影响。用BET、H2-TPR、TPD平衡法等考察了催化剂的比表面积、氧化还原性能、表面氧脱附性能,结果表明,添加变价助剂Mo、Fe和Cr氧化物会增加供氧数目,提高α-蒎烯转化率。MoO3-V2O5/Al2O3催化剂表面O 2-和O-物种较多,活性也较好,桃金娘烯醛收率较高。MoO3-V2O5/Al2O3催化剂的制备方法影响其催化性能,表面化学改性法制备的催化剂表面具有较多O2-和O-物种,有利于催化剂的活性提高,α-蒎烯的转化率达88.9%,桃金娘烯醛的收率为79.1%。