Fatty acid desaturase systems of hen liver and their inhibition by cyclopropene fatty acids

Hen liver preparations which desaturate stearic acid at the 9,10 position to form oleic acid have been found to desaturate other saturated fatty acids of carbon chain length from 12 to 20 and 22. The 9,10-monoenoic fatty acid of the same chain length as the substrate fatty acid is the major product formed. Minor amounts of the 10,11- and 11, 12-monoenoic acids are also formed. Maximum desaturation occurred with the C₁₄ fatty acid substrate and with the fatty acids C₁₇ and C₁₈, suggesting the presence of at least two desaturating systems. The cyclopropene fatty acids, sterculic and malvalic acids, inhibited the desaturation of all thefatty acids at the 9,10 position but desaturation at the 10,11 and 11, 12 positions was affected only slightly. The effect is not due to inhibition of the primary activating enzyme, the long chain acyl CoA synthetase. Sterculic acid is a more effective inhibitor than either malvalic acid or sterculyl alcohol, probably because these cyclopropene compounds do not block the desaturating site of the enzyme as completely as sterculic acid.     

迎接当今包处理的挑战

用户对激动人心的全新网络服务的需求,正在推动网络带宽和数据流量的增长.作为回应,网络正在向更有效的以包为基础的模式发展,这种模式使网络能够为用户提供其需要的服务,并开发出额外的收入来源.网络设备制造商必须掌握包处理技术,才能提供新的服务,从而应对不断增加的网络复杂性及适应不同网速的要求.     

Emission cross sections and spectroscopy of Ho3+ laserchannels in KGd(WO4)2 single crystal

The spectroscopic properties of Ho³⁺ laser channels in KGd(WO₄)₂ crystals have been investigated using optical absorption, photoluminescence, and lifetime measurements. The radiative lifetimes of Ho³⁺ have been calculated through a Judd-Ofelt (JO) formalism using 300-K optical absorption results. The JO parameters obtained were Ω₂=15.35×10^-20 cm², Ω ₄=3.79×10^-20 cm², Ω₆ =1.69×10^-20 cm². The 7-300-K lifetimes obtained in diluted (8·10¹⁸ cm^-3) KGW:0.1% Ho samples are: τ(⁵F₃)≈0.9 μs, τ( ⁵S₂)=19-3.6 μs, and τ(⁵F₅ )≈1.1 μs. For Ho concentrations below 1.5×10²⁰ cm^-3, multiphonon emission is the main source of non radiative losses, and the temperature independent multiphonon probability in KGW is found to follow the energy gap law τ_ph ^-1(0)=βexp(-αΔE), where β=1.4×10^-7 s^-1, and α=1.4×10³ cm. Above this holmium concentration, energy transfer between Ho impurities also contributes to the losses. The spectral distributions of the Ho³⁺ emission cross section σ_EM for several laser channels are calculated in σ- and π-polarized configurations. The peak a σ_EM values achieved for transitions to the ⁵I₈ level are ≈2×10^-20 cm² in the σ-polarized configuration, and three main lasing peaks at 2.02, 2.05, and 2.07 μm are envisaged inside the ⁵I₇→⁵I₈ channel     

On the Performance of TETRA DMO Short Data Service in Railway VANETs

In this paper we present the results of an empirical study investigating the performance of TETRA (TErrestrial Trunked RAdio) in a Vehicular Ad-hoc Network (VANET) for safety related railway traffic applications. The Short Data Service (SDS) of TETRA in the Direct Mode Operation (DMO) allows an infrastructure-less exchange of traffic relevant information between vehicles in range of the communication. The propagation channel in case of such a direct (base station free) communication between railway vehicles underlies specific effects, which are not equivalent to other well known terrestrial mobile scenarios. We will present measurements covering urban, suburban and rural environments along a regional railway network in the south of Bavaria. Beside different operational conditions such as front, rear, and flank approaches of trains, we investigated several topological scenarios on both, single and double track sections along the line. We will also discuss the observed characteristic changes in narrow band signal attenuation and Doppler spectra for passages through forests, hilly areas, stations and a tunnel. We determined statistics for the transmission delay of messages and the message erasure rates for single and multi user access on a single common carrier for different transmission intervals. Finally, the quality of service for the envisaged safety applications is assessed.     

Cracks in glass under triaxial conditions

This experimental work documents the mechanical evolution of synthetic glass (SON68) under compressive triaxial stresses (hydrostatic and deviatoric conditions). The experimental setup enabled to monitor and vary independently confining pressure (range: [0, 50] MPa) and axial stress (up to 680 MPa) at room temperature. An optimized set of sensors allowed us to perform measurements during the experiments of: (i) axial and radial deformation, (ii) P- and S-elastic wave velocities, and (iii) acoustic emissions. In addition, in some samples, initial crack densities up to a value of 0.24 were introduced by thermal cracking. We compare the original synthetic glass data set to results obtained in the same experimental conditions on thermally cracked glass and on a basaltic rock with similar petrophysical properties (porosity, chemistry).Stress-strain data depict original linear elastic glass properties even up to an axial stress of 680 MPa (under 15 MPa confining pressure). A strong strength decrease (370 MPa at 15 MPa confining pressure) is observed for thermally cracked samples. Elastic wave velocity data highlight that cracks are mostly closed at a confining pressure of ∼30 MPa. The basaltic rock seems to correspond to an intermediate state between an original and a thermally treated glass. In all samples, damage was accompanied by dynamic crack propagation, producing large magnitude acoustic emissions. Thanks to a continuous recorder, we could locate a number of acoustic emissions in order to image the microcracking pattern evolution prior to failure.     

Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows

Ten ruminally cannulated Holstein cows were used in a crossover design that investigated changes in ruminal bacterial populations in response to induction and recovery from diet-induced milk fat depression (MFD). Further, the effect on the ruminal microbiota of the cows with diet-induced milk fat depression inoculated with rumen contents from non-milk fat-depressed donor cows was evaluated. Milk fat depression was induced during the first 10 d of each period by feeding a low-fiber, high-starch, and high-polyunsaturated fatty acid diet (26.1% neutral detergent fiber, 28.1% starch, 5.8% total fatty acids, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. Induction was followed by a recovery phase, where all cows were switched to a high-fiber, low-starch, and low-polyunsaturated fatty acid diet (31.8% neutral detergent fiber, 23% starch, 4.2% total fatty acids, and 1.2% C18:2) and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8 kg/d for 6 d). Ruminal samples were collected at the end of induction (d 10) and during recovery (d 13, 16, and 28), separated to solid and liquid fractions, extracted for DNA, PCR- amplified for the V1-V2 region of the 16S rRNA gene, and analyzed for bacterial diversity. Results indicated that bacterial communities were different between fractions. In each fraction, differences were significant between the induction (d 10) and recovery (d 13, 16, and 28) periods; however, differences were less apparent with time during the recovery period. The MFD (d 10) was typified by a reduction in the relative sequence abundance of Bacteroidetes and an increase in the relative sequence abundance of Firmicutes and Actinobacteria across both fractions. At the genus level, relative sequence abundance of unclassified Lachnospiraceae, Butyrivibrio, Bulleidia, and Coriobacteriaceae were higher on d 10 and were positively correlated with trans-10,cis-12 CLA and the trans-10 isomer, suggesting their potential role in altered biohydrogenation reactions. A switch to the recovery diet resulted in a sharp increase in the Bacteroidetes lineages and a decrease in Firmicutes members on d 13; however, this shift appears to stabilize by d 28, indicating the restoration process for ruminal bacteria from an altered state is gradual and complex. Inoculation of 10% of rumen contents from non-MFD donor cows to MFD cows revealed this procedure had transient effects on only a few bacterial populations, and such effects disappeared after d 16 following cessation of inoculation. It can be concluded that alterations in milk FA profiles at induction are preceded by microbial alterations in the rumen driven by dietary changes.