On the masking of signals on immunoblots by cellular proteins

Functional magnetic resonance imaging (fMRI) is capable of detecting task-induced blood oxygenation changes using susceptibility sensitive pulse sequences such as gradient-recalled echo-planar imaging (EPI). The local signal increases seen in the time course are believed to be due to an increase in oxygen delivery that is incommensurate with oxygen demands. To help isolate the sources of functional signal changes, the authors have incorporated various forms of diffusion weighting into EPI pulse sequences to characterize the apparent mobility of the functionally modulated protons. Results suggest that the majority of the functional signal at 1.5 T arises from protons that have apparent diffusion coefficients that are approximately four or five times higher than that of brain tissue. This implies that significant functional signal sources are either protons within the vascular space or protons from the perivascular space that is occupied by cerebrospinal fluid.     

Reciprocating flow ion exchange part II: Application to saline water softening

The “hardness” components in sea water can be considerably reduced by means of a new economical fixed bed technique called “Reciprocating Flow Ion Exchange”, when used in conjunction with a closed cycle exchanger-evaporator system. Removal of 87.9% of the calcium and 54.5% of the magnesium in sea water has been obtained with an overall flow rate of 5.0 U. S. g. p. m./sq. ft. Results from bench scale and a 5,000 g. p. d. pilot plant were identical. The virtually continuous cyclic steady-state process requires a small amount of exchanger (0.5 to 2% of the usual ion exchange methods, such as conventional fixed beds or fluidized beds). The effects of variation in operating parameters are discussed.     

Studies on effects of dietary fatty acids as related to their position on triglycerides

This article reviews published literature on how the stereospecific structure of dietary triglycerides may affect lipid metabolism in humans. Animal studies have shown enhanced absorption of fatty acids in the sn-2 position of dietary triglycerides. Increasing the level of the saturated fatty acid palmitic acid in the sn-2 position (e.g., by interesterification of the fat to randomize the positions of the fatty acids along the glycerol backbone) has been shown in rabbits to increase the atherogenic potential of the fat without impacting levels of blood lipids and lipoproteins. In contrast, enhancing the level of stearic acid in the sn-2 position has not been found to affect either atherogenic potential or levels of blood lipids and lipoproteins in rabbit. Fatty acids other than palmitic and stearic have not been studied systematically with respect to possible positional effects. A limited number of human studies have shown no significant effects of interesterified fats on blood lipid parameters. However, it is unknown whether modifying the stereospecific structure of dietary triglycerides would affect atherogenicity or other long-term health conditions in humans. It is possible that incorporation of palmitic acid into the sn-2 position of milk fat is beneficial to the human infant (as a source of energy for growth and development) but not to human adults. Additional research is needed to determine whether processes like interesterification, which can be used to alter physical parameters of dietary fats (e.g., melting characteristics), may result in favorable or unfavorable long-term effects in humans.     

Multifrequency tympanometry in chinchillas

Multifrequency tympanometry (MFT), using probe frequencies ranging from 226-2,000 Hz, was performed on normal chinchillas to obtain normative data against which to compare results from animals with middle ear pathology. A series of validating experiments was conducted to determine the effects of anatomical alterations of the middle ear on MFT. These included artificially extending the ear canal, opening the bulla, injecting saline into the middle ear, and disrupting the ossicular chain. The results indicate that MFT characteristics of chinchilla ears are qualitatively similar to those observed in normal humans and patients with middle ear disease, and MFT provides information that is not available from the 226-Hz tympanogram.     

Iron Mobilization from Ferritin in Yeast Cell Lysate and Physiological Implications

Most in vitro iron mobilization studies from ferritin have been performed in aqueous buffered solutions using a variety of reducing substances. The kinetics of iron mobilization from ferritin in a medium that resembles the complex milieu of cells could dramatically differ from those in aqueous solutions, and to our knowledge, no such studies have been performed. Here, we have studied the kinetics of iron release from ferritin in fresh yeast cell lysates and examined the effect of cellular metabolites on this process. Our results show that iron release from ferritin in buffer is extremely slow compared to cell lysate under identical experimental conditions, suggesting that certain cellular metabolites present in yeast cell lysate facilitate the reductive release of ferric iron from the ferritin core. Using filtration membranes with different molecular weight cut-offs (3, 10, 30, 50, and 100 kDa), we demonstrate that a cellular component >50 kDa is implicated in the reductive release of iron. When the cell lysate was washed three times with buffer, or when NADPH was omitted from the solution, a dramatic decrease in iron mobilization rates was observed. The addition of physiological concentrations of free flavins, such as FMN, FAD, and riboflavin showed about a two-fold increase in the amount of released iron. Notably, all iron release kinetics occurred while the solution oxygen level was still high. Altogether, our results indicate that in addition to ferritin proteolysis, there exists an auxiliary iron reductive mechanism that involves long-range electron transfer reactions facilitated by the ferritin shell. The physiological implications of such iron reductive mechanisms are discussed.     

Weighted argument systems: Basic definitions, algorithms, and complexity results

We introduce and investigate a natural extension of Dung's well-known model of argument systems in which attacks are associated with a weight, indicating the relative strength of the attack. A key concept in our framework is the notion of an inconsistency budget, which characterises how much inconsistency we are prepared to tolerate: given an inconsistency budget β, we would be prepared to disregard attacks up to a total weight of β. The key advantage of this approach is that it permits a much finer grained level of analysis of argument systems than unweighted systems, and gives useful solutions when conventional (unweighted) argument systems have none. We begin by reviewing Dung's abstract argument systems, and motivating weights on attacks (as opposed to the alternative possibility, which is to attach weights to arguments). We then present the framework of weighted argument systems. We investigate solutions for weighted argument systems and the complexity of computing such solutions, focussing in particular on weighted variations of grounded extensions. Finally, we relate our work to the most relevant examples of argumentation frameworks that incorporate strengths.     

A 1-THz superconducting hot-electron-bolometer receiver for astronomical observations

In this paper, we describe a superconducting hot-electron-bolometer mixer receiver developed to operate in atmospheric windows between 800-1300 GHz. The receiver uses a waveguide mixer element made of 3-4-nm-thick NbN film deposited over crystalline quartz. This mixer yields double-sideband receiver noise temperatures of 1000 K at around 1.0 THz, and 1600 K at 1.26 THz, at an IF of 3.0 GHz. The receiver was successfully tested in the laboratory using a gas cell as a spectral line test source. It is now in use on the Smithsonian Astrophysical Observatory terahertz test telescope in northern Chile.     

Routing and fast protection in networks of long-reach PONs

This paper reports on proposed solutions to the recovery from faults in a generic class of networks, where customer access is achieved via long-reach passive optical networks (LR-PONs), with the majority of customers enjoying protection in the backhaul regime to two separate metro nodes. Initial modelling studies suggest that in the event of a cable failure or single equipment element failure, redirected data will almost always leave the transmitting node in under 50 ms. For more catastrophic failures (such as router failure or loss of a metro node), recovery might take between 100–200 ms. Reachability information for each LR-PON is discovered over each area of the network, and used to inform the redirection of traffic via tunnels. The scheme uses IP signalling to enable traffic re-routing, although the underlying services may be of any type (e.g. private line) — making the scheme separate from the service, customer or provider.