Enhanced healing of full-thickness diabetic wounds using bioactive glass and Yunnan baiyao ointments

In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yuunan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5 bioactive glass powder （16% weight） with Vaseline and different weight percentages of Yurman baiyao. Full-thickness defect wounds were created on the back of 130 SD rats and were randomly divided into 8 groups. The wound healing rates were calculated at 4, 7, 10, 14 and 21 days after surgery. The samples were harvested for further observations. Considering the wound closure rate, group 6 （with 5% Yuunan baiyao） has better wound healing performance than other diabetic groups. The lower inflammatory response was observed by gross observation and confirmed by the results of H＆E staining and TEM observation. Besides, the proliferation of fibroblasts, the formation of granulation tissue, as well as the vascularization, were improved in group 6 compared to other diabetic groups. All results suggest that bioactive glass and Yunnan baiyao ointments can accelerate the recovery of diabetes-impaired skin wounds, and comparing to other diabetic groups, group 6 （with 5% Yunnan baiyao） has better healing effect.     

溶胶–凝胶生物活性玻璃的结构特征及其对糖尿病创面修复作用的研究

采用熔融法和溶胶-凝胶法分别制备了生物活性玻璃45S5和SGBG,通过SEM、BET及XRD等方法对它们的微观结构进行了表征,并建立了SD大鼠糖尿病皮肤创面模型,通过对创面愈合时间、创面愈合率以及HE染色分析,探讨了生物活性玻璃对促进糖尿病难愈创面愈合的效果。结果表明,与凡士林组相比,生物活性玻璃组能加速创面愈合,且SGBG具有纳米结构,其比表面积更大,与45S5相比能缩短糖尿病创面的愈合时间,提高愈合速度。组织学分析表明生物活性玻璃能促进肉芽组织的生长。由此表明生物活性玻璃能促进大鼠糖尿病创面的修复,且具有纳米结构的SGBG效果更好。