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本文在考虑现有电压等级并预计20千伏可能列为我国标准电压情况下,按照六种电压组合,六种负荷密度,对某专区15000平方公里进行了规划设计。并以[6]作为引例,进一步说明220/110/20/0.38千伏电压组合是一种最优的电压等级组合,20千伏应例入我国标准电压等级。 相似文献
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相丽 《重庆科技学院学报(社会科学版)》2012,(3):186-187,191
提问是课堂教学中常用的一种教学方法。联系思想道德修养的课堂教学实际,分析了教师设计提问要注意的方面。认为教师不仅应该让学生"乐于问",而且要使学生"善于问"。介绍了激发学生进行有效提问的策略。 相似文献
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实时RT-PCR检测存活于乳中的单核细胞增多性李斯特菌 总被引:3,自引:0,他引:3
单增李斯特菌是一种可在低温下生长、能引起人畜共患病的食源性致病菌.为克服传统PCR检测的假阳性问题,有效的检测单增李斯特活菌,本研究提取单增李斯特菌的总RNA并进行反转录,以单增李斯特菌的必要毒力基因hlyA为靶基因,设计特异性引物和TaqMan探针进行实时PCR检测,研究了检测的特异性和灵敏度,考查了对人工污染牛乳进行检测的应用性.结果表明,所用引物和探针能较好的扩增目的基因,而对其他食源性致病菌无交叉反应.单增李斯特菌经1h增菌,可检出3×102CFU/ml.而经3h增菌,活菌检测限可达30CFU/ml.人工污染牛乳样品经6h增菌,检测限为17CFU/ml.此方法可应用于乳中单增李斯特菌的快速检测和污染状况调查. 相似文献
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Our previous studies demonstrated that Ce^4+could induce reactive oxygen species (ROS) burst as a signal to promote pacilitaxel biosynthesis in suspension cultured Taxus cuspidate cells. To further understand the mechanism of cerium ions inducing ROS burst, circular dichroism (CD), synchronous fluorescence, and electron paramagnetic resonance (EPR) were used to detect them inducing conformational change of horseradish peroxidase (HRP). Horseradish peroxidase activity was reduced by 78% by 0.1 mmol/L Ce^4+, whereas it was only reduced by 28% by 0.1 mmol/L Ce^3+. Circular dichroism spectra showed that the percentage of transition from helical content and other structure to β strands and flturns was 23.1 when induced by Ce^4+, whereas it was only 13.2 when induced by Ce^3+. In synchronous fluorescence spectra, Ce^4+ led to red shift and intensity-elevation of tryptophan fluorescence emission maximum, whereas in the case of Ce^3+, the results were a contrast to the above. Furthermore, g factor (gx and gy) in electron paramagnetic resonance (EPR) induced by Ce^4+ and Ce^3+ was significantly different. These results indicated that the different valence of cerium ion induced various conformations of HRP, and Ce^4+ was more effective than Ce^3+. This suggested that Ce^4+ affected the burst of ROS through changing the conformation of oxidoreductase. 相似文献
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