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1.
Prof. Dr. Britto S. Sandanaraj Pavankumar Janardhan Bhandari Mullapudi Mohan Reddy Akshay Bhagwan Lohote Dr. Bankanidhi Sahoo 《Chembiochem : a European journal of chemical biology》2020,21(3):408-416
The custom design of protein–dendron amphiphilic macromolecules is at the forefront of macromolecular engineering. Macromolecules with this architecture are very interesting because of their ability to self-assemble into various biomimetic nanoscopic structures. However, to date, there are no reports on this concept due to technical challenges associated with the chemical synthesis. Towards that end, herein, a new chemical methodology for the modular synthesis of a suite of monodisperse, facially amphiphilic, protein–dendron bioconjugates is reported. Benzyl ether dendrons of different generations (G1–G4) are coupled to monodisperse cetyl ethylene glycol to form macromolecular amphiphilic activity-based probes (AABPs) with a single protein reactive functionality. Micelle-assisted protein labeling technology is utilized for site-specific conjugation of macromolecular AABPs to globular proteins to make monodisperse, facially amphiphilic, protein–dendron bioconjugates. These biohybrid conjugates have the ability to self-assemble into supramolecular protein nanoassemblies. Self-assembly is primarily mediated by strong hydrophobic interactions of the benzyl ether dendron domain. The size, surface charge, and oligomeric state of protein nanoassemblies could be systematically tuned by choosing an appropriate dendron or protein of interest. This chemical method discloses a new way to custom-make monodisperse, facially amphiphilic, protein–dendron bioconjugates. 相似文献
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监测正电源的电流时,通常使用高边检流放大器。然而,对于ISDN、电信电源,通常需要一个工作在负电源的检流放大器。本文介绍了一种采用MAX4460单电源仪表放大器设计负压检流放大器的方法。 相似文献
4.
P. K. Khare J. M. Keller M. S. Gaur Ranjeet Singh S. C. Datt 《Polymer International》1994,35(4):337-343
The electrical conductivity of solution-grown ethyl cellulose (EC) films, 5–30 μm thick, has been studied in the sandwich configuration (metal–EC–metal) as a function of iodine concentration from 0.5 to 5.0 wt% ratio. The studies were conducted in the temperature range 333–383 K, while the field was varied over the range (3.0–5.5) × 104V/cm. Aluminium was used as the lower electrode, while the upper electrode was of Al, Ag, Cu, Au or Sn. Certain transient effects such as a large burst of current immediately after the application of field were observed. An attempt was made to identify the nature of the current by comparing the observed dependence on electric field, electrode material and temperature with the respective characteristic features of the existing theories of electrical conduction. The results show that the electrical conduction follows Ohm's law at lower fields, while at higher fields, space-charge limited current (SCLC) was observed. It was also found that Richardson–Schottky emission was responsible, to some extent, for the transport of charge carriers in the polymer. The conductivity of the films increased on doping with iodine. The dopant molecules are considered to act as additional trapping centes and provide links between the polymer molecules in the amorphous region, thus resulting in the formation of charge transfer complexes. 相似文献
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Inventory aggregation, also called Risk Pooling, is one of the most efficient ways to reduce the level of safety stocks thereby reducing inventory across the supply chain. Determining the best level of aggregation is a difficult problem and needs extensive study of all the possible scenarios that can affect this decision. Minimizing costs in a supply chain is no longer the sole priority of businesses. Maintaining a high level of responsiveness is also considered equally important. The conflicting nature of these two criteria makes the solution of the problem difficult. In this paper, we develop a bi-criteria nonlinear stochastic integer programming model to determine the best supply chain distribution network to meet customer demands, where minimizing costs while maintaining high levels of responsiveness is important. We develop a two-stage optimization algorithm to solve this problem. 相似文献
7.
Electrical conduction in non-metallic rare-earth solids 总被引:1,自引:0,他引:1
Schematic energy band diagrams for the genesis of charge carriers in non-metallic rare-earth solids have been presented. It has been shown that positions of 4f bands have significant effect on the genesis and nature of charge carriers, their conduction mechanism and magnitude of electrical conductivity () and Seebeck coefficient (S) of the solid. Relevant relations have been given for both and S in different situations. Experimental data on rare-earth sesquioxides (R2O3), rare-earth tungstates [R2(WO4)3] and rare-earth molybdates [R2(MoO4)3] in the intrinsic range have been explained as examples for the validity of energy band diagrams. 相似文献
8.
Akshay Atwe Ankur Gupta Rishi Kant Mainak Das Ishan Sharma Shantanu Bhattacharya 《Microsystem Technologies》2014,20(7):1373-1381
We report a novel pH-sensitive hydrogel based micro-valve for metered flow that has applications in a laboratory made “Intelligent valving system”. The hydrogel solution was prepared through Chitosan and poly vinyl alcohol in acetic acid and crystallized using gluteraldehyde as the crosslinking agent in the form of thin wafers and it was found to be very sensitive to pH changes. The pore structure of hydrogel was investigated through Field Emission Scanning Electron Microscopy and thin wafers of the gel were physically placed inside PDMS microchannels. Flow metering in these channels was observed by controlled expansion of the hydrogel plug till complete valving was realized. This valving device was further precisely characterized with micro Particle Image Velocimetry using a solution containing fluorescent polymeric micro beads. The principle advantage of this hydrogel device is the smaller range of pH (varying between pH 3 and 7) over which the valving response is observed. 相似文献
9.
Akshay Deepak David Fernández-Baca Srikanta Tirthapura Michael J. Sanderson Michelle M. McMahon 《Knowledge and Information Systems》2014,41(3):559-590
The problem of mining collections of trees to identify common patterns, called frequent subtrees (FSTs), arises often when trying to interpret the results of phylogenetic analysis. FST mining generalizes the well-known maximum agreement subtree problem. Here we present EvoMiner, a new algorithm for mining frequent subtrees in collections of phylogenetic trees. EvoMiner is an Apriori-like levelwise method, which uses a novel phylogeny-specific constant-time candidate generation scheme, an efficient fingerprinting-based technique for downward closure, and a lowest-common-ancestor-based support counting step that requires neither costly subtree operations nor database traversal. Our algorithm achieves speedups of up to 100 times or more over Phylominer, the current state-of-the-art algorithm for mining phylogenetic trees. EvoMiner can also work in depth-first enumeration mode to use less memory at the expense of speed. We demonstrate the utility of FST mining as a way to extract meaningful phylogenetic information from collections of trees when compared to maximum agreement subtrees and majority-rule trees—two commonly used approaches in phylogenetic analysis for extracting consensus information from a collection of trees over a common leaf set. 相似文献
10.
The declining quality of the public realm in urban environment is a matter of concern and is found to be encroached upon in Indian cities. There is an urgent need to address this issue, and it is challenging for urban designers. Adopting an analytic framework addressing this ever-changing nature of the public realm in practice would be instrumental in improving the quality of urban life at large. The public realm in an urban environment is considered as a complex system to observe the macro-level behaviour—emergent properties which are either encouraging or discouraging for encroachments assumed to be the function of micro-level behaviour—elementary properties of interconnected and interdependent individual elements. Owing to the impossibility of experimenting with these emergent properties in the real world, an agent-based model—“Emergent Properties of Public Realm 3.1”—was developed in NetLogo 4.1.3. The typical cases representing the encroached and the un-encroached public realm were identified in the real world and experiments were conducted with varied interaction constants assumed to represent the pace of change in an urban environment. The output generated by the agent-based model was analyzed with respect to the real world. 相似文献