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排序方式: 共有85条查询结果,搜索用时 15 毫秒
1.
F Abadía-Molina R Calvente R Carmona R Luján F Abadía-Fenoll 《Canadian Metallurgical Quarterly》1993,8(2):297-304
In this study chick embryo optic cups at HH stage 13 of development were analyzed under normal conditions and after inoculation with colchicine for 1, 2, 4, and 8 h. Several changes were seen after these periods of treatment: 1) modifications of the structure, with thicker regions in the cup and a general decrease in the total volume according to the duration of exposure to the drug (about 4 times less than normal, 5,035 x 10(3) microns 3 vs 1,334 x 10(3) microns 3 after 8 h of treatment); 2) enlargement of the ventricular cavity and its closure, due to failure of approximation of retinal and pigmentary layers; 3) failure of lens development, with delay and impairment of pit formation and deformation of all structures; lens volume was less than normal (about 4 times less, 2,148 x 10(3) microns 3 vs 658 x 10(3) microns 3 after 8 h of treatment); 4) a general segregation of the cells making up the structure, principally in the more active proliferating zones. The local alterations found are described. 相似文献
2.
基于BP神经网络的模糊PID风量控制 总被引:1,自引:0,他引:1
针对煤矿主风机通风系统多变量、非线性、时变滞后性等问题,提出一种基于BP神经网络的模糊PID算法。该算法综合神经网络、模糊控制与PID调节的各自优点,既具有神经网络的自学习和自适应能力,又具有模糊控制的非线性控制作用,同时兼备PID调节的广泛性。仿真结果表明,该算法的响应速度、稳态精度均优于传统的PID调节,取得比较理想的控制效果。 相似文献
3.
Ekaterina M. Dvorianinova Nadezhda L. Bolsheva Elena N. Pushkova Tatiana A. Rozhmina Alexander A. Zhuchenko Roman O. Novakovskiy Liubov V. Povkhova Elizaveta A. Sigova Daiana A. Zhernova Elena V. Borkhert Dmitry N. Kaluzhny Nataliya V. Melnikova Alexey A. Dmitriev 《International journal of molecular sciences》2022,23(21)
High-quality genome sequences help to elucidate the genetic basis of numerous biological processes and track species evolution. For flax (Linum usitatissimum L.)—a multifunctional crop, high-quality assemblies from Oxford Nanopore Technologies (ONT) data were unavailable, largely due to the difficulty of isolating pure high-molecular-weight DNA. This article proposes a scheme for gaining a contiguous L. usitatissimum assembly using Nanopore data. We developed a protocol for flax nuclei isolation with subsequent DNA extraction, which allows obtaining about 5 μg of pure high-molecular-weight DNA from 0.5 g of leaves. Such an amount of material can be collected even from a single plant and yields more than 30 Gb of ONT data in two MinION runs. We performed a comparative analysis of different genome assemblers and polishers on the gained data and obtained the final 447.1-Mb assembly of L. usitatissimum line 3896 genome using the Canu—Racon (two iterations)—Medaka combination. The genome comprised 1695 contigs and had an N50 of 6.2 Mb and a completeness of 93.8% of BUSCOs from eudicots_odb10. Our study highlights the impact of the chosen genome construction strategy on the resulting assembly parameters and its eligibility for future genomic studies. 相似文献
4.
Daniel Alberto Sánchez Gabriela Marta Tonetto María Luján Ferreira 《Journal of the American Oil Chemists' Society》2014,91(2):261-270
New avenues to add value to glycerol are currently being explored. One of them is the synthesis of structured lipids through glycerol esterification. In this work we have analyzed the recovery and purification of dicaprin obtained by esterification of glycerol with capric acid (C) in heptane, mediated by Lipozyme RM IM. This is an intermediate step to obtain lipids MLM. In the first stage, the diglyceride synthesis MGM (being G a central HC–OH) was carried out. When M = C, the diglyceride is CGC. Recovery of the diglyceride CGC is required to carry out the esterification of the sn-2 position with palmitic acid (P), thus obtaining the triglyceride CPC. Different solvents were evaluated using Ecofac 1.0 (a molecular design software solvent) through a theoretical approach to explore the best solvents for the acylglycerides separation. Then, the performance of the selected solvents to separate dicaprin from mono and tricaprin was experimentally studied in a liquid–liquid extraction process. Previously, the remaining fatty acid had been neutralized. With liquid–liquid extraction in three simple steps, using ethanol/water, 94 % of the dicaprin obtained by enzymatic esterification was recovered with a purity of 89 % (wt%). It was also possible to obtain dicaprin with a purity of 97 % but with a yield of 56 %. 相似文献
5.
Sofía Valla Nourhan Hassan Daiana Lujn Vitale Daniela Madanes Fiorella Mercedes Spinelli Felipe C. O. B. Teixeira Burkhard Greve Nancy Adriana Espinoza-Snchez Carolina Cristina Laura Alaniz Martin Gtte 《International journal of molecular sciences》2021,22(11)
Glycosaminoglycans (GAGs) and proteoglycans (PGs) are major components of the glycocalyx. The secreted GAG and CD44 ligand hyaluronic acid (HA), and the cell surface PG syndecan-1 (Sdc-1) modulate the expression and activity of cytokines, chemokines, growth factors, and adhesion molecules, acting as critical regulators of tumor cell behavior. Here, we studied the effect of Sdc-1 siRNA depletion and HA treatment on hallmark processes of cancer in breast cancer cell lines of different levels of aggressiveness. We analyzed HA synthesis, and parameters relevant to tumor progression, including the stem cell phenotype, Wnt signaling constituents, cell cycle progression and apoptosis, and angiogenic markers in luminal MCF-7 and triple-negative MDA-MB-231 cells. Sdc-1 knockdown enhanced HAS-2 synthesis and HA binding in MCF-7, but not in MDA-MB-231 cells. Sdc-1-depleted MDA-MB-231 cells showed a reduced CD24-/CD44+ population. Furthermore, Sdc-1 depletion was associated with survival signals in both cell lines, affecting cell cycle progression and apoptosis evasion. These changes were linked to the altered expression of KLF4, MSI2, and miR-10b and differential changes in Erk, Akt, and PTEN signaling. We conclude that Sdc-1 knockdown differentially affects HA metabolism in luminal and triple-negative breast cancer model cell lines and impacts the stem phenotype, cell survival, and angiogenic factors. 相似文献
6.
Daiana Garcia Germán Barros Sofía Chulze Antonio J. Ramos Vicente Sanchis Sonia Marín 《Journal of the science of food and agriculture》2012,92(15):2952-2959
BACKGROUND: Fusarium graminearum and F. verticillioides are two very important mycotoxigenic species as they cause diverse diseases in crops. The effects of constant and cycling temperatures on growth and mycotoxin production of these species were studied on soybean based medium and on irradiated soya beans. RESULTS: F. graminearum grew better when was incubated at 15, 20 and 15–20 °C (isothermal or cycling temperature) during 21 days of incubation. Maximum levels of zearalenone and deoxynivalenol (39.25 and 1040.4 µg g?1, respectively) were detected on soya beans after 15 days of incubation and the optimal temperature for mycotoxin production was 15 °C for zearalenone and 20 °C for deoxynivalenol. F. verticillioides grew better at 25 °C in culture medium and at 15/20 °C and 15/25 °C on soybean seeds. Fumonisin B1 was produced only in culture medium, and the maximum level (7.38 µg g?1) was found at 15 °C after 7 days of incubation. CONCLUSION: When growth and mycotoxin production under cycling temperatures were predicted from the results under constant conditions, observed values were different from calculated for both species and substrate medium. Therefore, care should be taken if data at constant temperature conditions are to be extrapolated to real field conditions. Copyright © 2012 Society of Chemical Industry 相似文献
7.
Maria Alejandra Luján Escalante 《CoDesign》2019,15(1):59-74
ABSTRACTFor academic design programmes and research councils, finding mechanisms to measure impact and valuate co-design research projects is very difficult. The metric parameters – number of attendees, volume, shape, length – have shown to be insufficient. The subject matter, the subjects and matters, do not easily fit within these parameters, which fail to translate the value of a ‘constellation of affections’ that emerge throughout co-design processes. Emphasis on quantifiable impact derives from particular ways of understanding production, reproduction, and dissemination of knowledge. This paper understands academic design programmes as a technology to discuss axiology in co-design practices. This paper re-configures a techno-logic of value; from chain of value to value constellation, intersecting a co-design case study with readings of Simondon’s philosophy of technology and Barad’s agential realism. Ageing Playfully was a knowledge exchange project from The Creative Exchange programme, that involved people living with dementia and their caregivers co-designing interventions to boost mobility and well-being. At the core, this paper, explores an axiological move that informs a framework of emergence, a way of thinking in methods to account for the value of co-design as site of emergence. 相似文献
8.
9.
J. Galán T. Sánchez-Rodríguez C. Luján-Martínez M. Pedro R. G. Carvajal A. López-Martín 《Analog Integrated Circuits and Signal Processing》2010,62(1):1-8
A fourth-order low-pass channel filter for a zero-IF Bluetooth receiver is presented. It employs two cascaded multiple feedback
biquads and active resistors made by quasi-floating gate MOS transistors in triode region for highly linear continuous tuning.
Power consumption is strongly reduced using a gain-enhanced class-AB single-stage opamp together with a filter topology that
only requires a single opamp for each biquad. The filter has been fabricated in a 0.18 μm CMOS technology and consumes 290 μW
from a 1.2 V supply. 相似文献
10.
Experiments with micro-fin tube in single phase 总被引:1,自引:0,他引:1
Jacqueline Biancon Copetti Mario Henrique Macagnan Daiana de Souza Rejane De Csaro Oliveski 《International Journal of Refrigeration》2004,27(8):876-883
This work shows heat transfer and friction characteristics for water single-phase flow in micro-fin tubes. The analysis of thermal and hydraulic behavior from a laminar to a turbulent flow was carried out in an experimental setup with a 9.52 mm diameter micro-fin tube. The tube was wrapped up with an electrical resistance tape to supply a constant heat flux to its surface. Different operational conditions were considered in the heating tests. The inlet and outlet temperatures, differential wall temperatures along the tube, pressure drop and flow rate were measured. The relationships of heat flux and flow rate with heat transfer coefficient and pressure drop were analyzed. Under the same conditions, comparative experiments with an internally smooth tube were conducted. The micro-fin tube provides higher heat transfer performance than the smooth tube (in turbulent flow hmicro-fin/hsmooth=2.9). In spite of the increase in pressure drop (Δpmicro-fin/Δpsmooth=1.7) the heat transfer results were significantly higher (about 80%). This shows the advantages of this enhanced configuration in thermal performance related to conventional tubes. The smooth tube results were validated by the comparison with the Dittus–Boelter and Gnielinski correlations. For the micro-fin tube an empirical correlation to the heat transfer coefficient adjusted from the set of measured data is proposed. The values obtained are in conformity with experimental results. 相似文献