Antidiabetic Drugs in the Treatment of Alzheimer’s Disease

The public health burden of type 2 diabetes mellitus and Alzheimer’s disease is steadily increasing worldwide, especially in the population of older adults. Epidemiological and clinical studies suggest a possible shared pathophysiology between the two diseases and an increased risk of AD in patients with type 2 diabetes mellitus. Therefore, in recent years, there has been a substantial interest in identifying the mechanisms of action of antidiabetic drugs and their potential use in Alzheimer’s disease. Human studies in patients with mild cognitive impairment and Alzheimer’s disease have shown that administration of some antidiabetic medications, such as intranasal insulin, metformin, incretins, and thiazolidinediones, can improve cognition and memory. This review aims to examine the latest evidence on antidiabetic medications as a potential candidate for the treatment of Alzheimer’s disease.     

Differentiation of lactococci from 2 Greek cheeses with protected designation of origin by phenotypic criteria and RAPD-PCR

Abstract: Seventy‐six lactococci isolates from 2 protected designation of origin (PDO) cheeses were studied for their acidification ability, proteolytic activity, and inhibitory activities as well as their intraspecies characterization by randomly amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR). Fifty‐two of them were characterized as Lactococcus lactis subsp. lactis by the SDS‐PAGE of whole‐cell proteins. The test strains increased the amount of acid in milk from 6 to 24 h as well as the quantities of amino acids on incubation for 4 d. The majority of the isolates degraded preferentially α_s‐casein. The isolates from Feta differed from those of Graviera Kritis in respect of β‐casein degradation. This fragment was either not degraded or underwent a small degradation by lactococci from Feta. A stronger intensity of acidification for the isolates from Feta and a higher casein breakdown ability for those from Graviera Kritis were also recorded. Lactococci from Feta and Graviera Kritis inhibited, preferentially, the growth of Escherichia coli O157:H7, and Yersinia enterocolitica, respectively. A high heterogeneity among the isolates according to RAPD‐PCR was determined, as well as grouping of the isolates according to their source of isolation. Selected isolates from each cheese could be used as starters to make either Feta or Graviera Kritis.     

Protein stability function relations: native β‐lactoglobulin sulphhydryl–disulphide exchange with PDS

Intermolecular sulphhydryl–disulphide exchange with β‐lactoglobulin dimer occurs when this dissociates to form monomers exposing two SH groups. This notion is re‐evaluated in the light of recent structural data suggesting that the degree of SH group exposure in β‐lactoglobulin is unaffected by dissociation. β‐Lactoglobulin was treated with 2,2′‐dipyridyl disulphide (PDS). The rate of sulphhydryl–disulphide exchange was measured at sub‐denaturation temperatures of 25–60 ° C. Parallel studies were conducted by reacting PDS with reduced glutathione (GSH). The SH group of GSH was up to 31 000 times more reactive than β‐lactoglobulin. At pH 7 the reaction activation enthalpy (ΔH^#) and entropy (ΔS^#) was 26 kJ mol⁻¹ and −100 J mol⁻¹ K⁻¹ respectively for GSH. For β‐lactoglobulin, ΔH^# was 157.2 kJ mol⁻¹ and ΔS^# was 254 J mol⁻¹ K⁻¹. At pH 2.6, ΔH^# was 14.4 kJ mol⁻¹ and ΔS^# was −213 J mol⁻¹ K⁻¹ for GSH. The corresponding results for β‐lactoglobulin were 20.3 kJ mol⁻¹ and −147 J mol⁻¹ K⁻¹. These and other thermodynamic results are discussed in terms of the effects of β‐lactoglobulin conformational structure and stability on SH group reactivity. For native β‐lactoglobulin at neutral pH, intermolecular sulphhydryl–disulphide exchange appears to involve the dissociated monomer. SH group activation probably arises from the lower structural stability of the monomer relative to the dimer. At pH 2.6 the mechanism of SH–disulphide exchange does not require protein dissociation and probably involves breathing motions or localised changes in protein structure. © 2000 Society of Chemical Industry     

Hydrodeoxygenation of HMF over Pt/C in a continuous flow reactor

The three‐phase hydrodeoxygenation reaction of 5‐hydroxymethylfurfural (HMF) with H₂ was studied over a 10 wt % Pt/C catalyst using both batch and flow reactors, with ethanol, 1‐propanol, and toluene solvents. The reaction is shown to be sequential, with HMF reacting first to furfuryl ethers and other partially hydrogenated products. These intermediate products then form dimethyl furan (DMF), which in turn reacts further to undesired products. Furfuryl ethers were found to react to DMF much faster than HMF, explaining the higher reactivity of HMF when alcohol solvents were used. With the optimal residence time, it was possible to achieve yields approaching 70% in the flow reactor with the Pt/C catalyst. Much higher selectivities and yields were obtained in the flow reactor than in the batch reactor because side products are formed sequentially, rather than in parallel, demonstrating the importance of choosing the correct type of reactor in catalyst screening. © 2014 American Institute of Chemical Engineers AIChE J, 61: 590–597, 2015     

Effects of Monoterpenoids, Acting Alone or in Pairs, on Seed Germination and Subsequent Seedling Growth

We compared the potential allelopathic activity of 47 monoterpe- noids of different chemical groups, by estimating their effect on seed germination and subsequent growth of Lactuca sativa seedlings. Apart from individual compounds, eleven pairs at different proportions were also tested. As a group, the hydrocarbons, except for (+)-3-carene, were the least inhibitory. Of the oxygenated compounds, the least inhibitory were the acetates; whenever the free hydroxyl group of an alcohol turned into a carboxyl group, the activity of the resulting ester was markedly lower (against both germination and seedling growth). Twenty-four compounds were extremely active against seedling growth (inhibiting it by more than 85%), but only five against seed germination. The compounds that were most active against both processes belonged to the groups of ketones and alcohols; they were terpinen-4-ol, dihydrocarvone, and two carvone stereoisomers. We used a model to investigate whether compounds acted independently when applied in pairs. The combined effect varied. In half of the cases, it followed the pattern expected under the assumption of independence; in the rest, either synergistic or antagonistic interactions were found in both germination and elongation. However, even in cases of synergistic interactions, the level of inhibition was not comparable to that of a single extremely active compound, unless such a compound already participated in the combination. The specific structural factors that operate and determine the activity of monoterpenoids still remain rather obscure. The same holds true for the combined effect; its character cannot in general be predicted on the basis of individual compounds acting alone.     

Kinetic and reaction mechanisms of calcium bisulfite catalytic oxidation

In this paper, the oxidation of calcium bisulfite in the presence of ferrous ions as a catalyst is studied in a laboratory scale apparatus at a fixed oxygen partial pressure (21.3 kPa) and at a temperature of 45 °C. The analysis of the experimental results, carried out by using the theory of mass transfer with chemical reaction, indicates that the slow reaction regime has been explored, and the transition from the kinetic to the diffusional subregime identified. A kinetic analysis allows to develop a reaction rate equation, assuming that the bisulfite oxidation in the presence of ferrous ions follows a parallel reaction mechanism; the resulting overall reaction rate has been found to be of first order with respect to ferrous ion concentration and of three halves with respect to bisulfite concentration. Moreover, the catalytic effect of ferrous ions has been compared with that of manganese ions previously studied, showing that ferrous is a catalyst more active than manganese.     

国外低特征信号富燃料推进剂的研究进展

综述了国外低特征信号富燃料推进剂的研究进展,归纳了研究低特征信号富燃料推进剂的技术途径。认为碳氢富燃料推进剂具有低特征信号的特点,是研究低特征信号富燃料推进剂的基础,采用非金属燃料是降低推进剂一次烟烟雾特征信号的有效途径,通过添加剂消除HCl是降二次烟的有效手段。对我国低特征信号富燃料推进剂的发展提出了几点建议。     

Strong and privacy-friendly management of federated identities for service provision over UMTS

Mobile subscribers who wish to mutually authenticate to service providers on the Internet utilize existing identity management mechanisms, such as Microsoft .net passport, overlooking the existing trust relationship between the subscriber and the 3G mobile operator and increasing network resources consumption, in an environment that requires security mechanisms that are as lightweight as possible. Furthermore, knowledge as well as the possession of an item, does not distinguish a person uniquely, revealing an inherent security weakness of pin authentication mechanisms. This paper proposes a protocol (3GbioId) for implementing strong identity management for Internet applications over 3G mobile networks. 3GBioId introduces biometrics, as well as the principles of the Liberty Alliance, into the 3G mobile security architecture, targeting to a more effective, secure and lightweight identity management alternative to the existing protocols. The results of a security, privacy, performance, usability and complexity evaluation indicate 3GbioId’s benefits and limits.     

eInvoke: Secure e-Invoicing based on web services

Electronic Invoicing services (e-Invoicing) will have a pivotal role in all the stages of handling Value Added Tax (VAT) for European Member States. Through a systematic introduction of e-invoicing, tax administrators will be able to implement new tools and procedures to carry out alternative controls that are less intrusive on the trading partners. Nevertheless, successful European e-invoicing implementations need to be in compliance with the corresponding European Directive 2001/115/EC. Most contemporary e-Invoicing implementations are proprietary and based on EDI, thus demonstrating great deficiencies. This paper presents an open electronic invoicing system named eInvoke, based on XML, XML cryptography and Web Services, that addresses all security requirements imposed by the Directive. Our e-Invoicing system has been accepted by “CEN/ISSS e-Invoicing Focus Group on Standards and Developments on electronic invoicing relating to VAT Directive 2001/115/EC” and its summary appears in CEN 2003 report [13] (pg 79–85), forwarded to EC as a recommendation. Alexandros Kaliontzoglou holds a Degree in Electrical and Computer Engineering from the National Technical University of Athens (NTUA), Greece. Since 2001 he is a PhD candidate in the area of Network and Information Systems Security at the Telecommunications laboratory in the School of Electrical and Computer Engineering of NTUA. Since April 2000 he has been working for Expertnet S.A. as a security engineer specializing at Web Technologies and network applications, and he has been active both in European research projects in the 5th and 6th Framework Programme (eMayor, Intelcities, SELIS, Reshen, La Mer, TSEC, WebSig) and projects of the Greek private sector. His research interests focus in the area of IT Security, Service Oriented Architectures, Web Services, Software Engineering, e-Government, e-Commerce and Public Key Infrastructures. Pelagia Boutsi has obtained the Degree in Informatics from the University of Piraeus, Greece, in November 2001. Since April 2002, she is a PhD candidate in the area of Security Information at the Computer Science Department of University of Piraeus. Since September 2001 she is employed at Expertnet S.A. as member of the Technical Department. Her current research interests are in the fields of PKIs, XML and XML Security. She has participated in European research projects and projects of the private sector. Despina Polemi has obtained the Degree in Applied Mathematics from Portland State University(USA) in 1984, Ph.D. in Applied Mathematics (Coding Theory) from City University of New York (Graduate Center) in 1991. She held teaching positions (1984– 1995) in Queens College and Baruch College of City University of NewYork. From 1991 to 1996 was assistant professor (tenure track) in State University of New Yorkat Farmingdale in the department of Mathematics. During 1996–2002 she was an associate researcher in ICCS. From 2000 to 2003 she acted as President of the BoD in a security consulting company Expertnet (www.expertnet.net.gr) and technical manager of the company from 2000–2004. She nowa Professor in the University of Piraeus R&D department. Her current research interests are in the fields of cryptology, security and e-business. She has over ninety publications in the above areas. She has received many research grants from various organizations such as the Danish Research Foundation, MSI Army Research Office/Cornell University, IEEE, State University of New York (SUNY), and The Graduate School of City University of New York (CUNY). She has been project manager (PM)/technical manger (TM) in security projects of various programmes such as National Security Agency (NSA), Dr. Nuala McGann Drescher Foundation, Greek Ministry of Defense, INFOSEC TELEMATICS for Administrations (COSACC), the Fifth Framework IST Programme (HARP, BEE, SEED, WebSig, TSEC, CORAS, RESHEN, SEED, La Mer, SECRETS) and the 6FP (e-Mayor, Intelcities, BIOSEC, SELIS). She participated in the EC security projects of the programs COST, ACTS, and NATOs’ security projects. She is a member of IEEE. She serves as an evaluator, reviewer and expert in the European Commission and consultant for the FP6.