Subacute Plasmodium falciparum malaria in 43 patients returning from areas with chloroquine in Africa

PURPOSE: To identify clinical and biological features of subacute falciparum malaria, risk factors, and to evaluate the efficacy of curative treatment. PATIENTS AND METHODS: Diagnostic criteria were the association of apyrexia, anemia, little or no parasitemia and a high titer of anti-Plasmodium antibodies. Forty-three cases were observed in subjects returning from chloroquine-resistant areas in Africa. They were matched with controls for age, country of residence and duration of stay. Controls were missionaries who attended our unit for a routine medical check-up during the study period. RESULTS: The clinical presentation and biological features were similar to "malarial cachexia", a condition mainly described in non-immune children in endemic areas. Splenomegaly was present in 58% of the patients. Biological features included little or no parasitemia, an overall decrease in the blood cell count, an increased erythrocyte sedimentation rate and a high titer of anti-Plasmodium antibodies. This syndrome was not correlated with the frequency of chloroquine resistance, the area of stay (urban or rural) or to the kind of chemoprophylaxis. CONCLUSIONS: This study describes subacute resistant falciparum malaria in patients who had prolonged stay in chloroquine-resistant areas of Africa associating splenomegaly, cytopenia and a low or absent parasitemia. Subacute chloroquine-resistant malaria could be due to host factors which remained to be determined by prospective immunological studies. Curative treatment with mefloquine is effective.     

Long COVID: Association of Functional Autoantibodies against G-Protein-Coupled Receptors with an Impaired Retinal Microcirculation

Long COVID (LC) describes the clinical phenotype of symptoms after infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnostic and therapeutic options are limited, as the pathomechanism of LC is elusive. As the number of acute SARS-CoV-2 infections was and is large, LC will be a challenge for the healthcare system. Previous studies revealed an impaired blood flow, the formation of microclots, and autoimmune mechanisms as potential factors in this complex interplay. Since functionally active autoantibodies against G-protein-coupled receptors (GPCR-AAbs) were observed in patients after SARS-CoV-2 infection, this study aimed to correlate the appearance of GPCR-AAbs with capillary microcirculation. The seropositivity of GPCR-AAbs was measured by an established cardiomyocyte bioassay in 42 patients with LC and 6 controls. Retinal microcirculation was measured by OCT–angiography and quantified as macula and peripapillary vessel density (VD) by the Erlangen-Angio Tool. A statistical analysis yielded impaired VD in patients with LC compared to the controls, which was accentuated in female persons. A significant decrease in macula and peripapillary VD for AAbs targeting adrenergic β2-receptor, MAS-receptor angiotensin-II-type-1 receptor, and adrenergic α1-receptor were observed. The present study might suggest that a seropositivity of GPCR-AAbs can be linked to an impaired retinal capillary microcirculation, potentially mirroring the systemic microcirculation with consecutive clinical symptoms.     

Serum Proteomics in Patients with Head and Neck Cancer: Peripheral Blood Immune Response to Treatment

In this real-world study, the aims were to prospectively evaluate the expression of inflammatory proteins in serum collected from head and neck cancer patients before and after treatment, and to assess whether there were differences in expression associated with treatment modalities. The mixed study cohort consisted of 180 patients with head and neck cancer. The most common tumor sites were the oropharynx (n = 81), the oral cavity (n = 53), and the larynx (n = 22). Blood tests for proteomics analysis were carried out before treatment, 7 weeks after the start of treatment, and 3 and 12 months after the termination of treatment. Sera were analyzed for 83 proteins using an immuno-oncology biomarker panel (Olink, Uppsala, Sweden). Patients were divided into four treatment groups: surgery alone (Surg group, n = 24), radiotherapy with or without surgery (RT group, n = 94), radiotherapy with concomitant cisplatin (CRT group, n = 47), and radiotherapy with concomitant targeted therapy (RT Cetux group, n = 15). For the overall cohort, the expression levels of 15 of the 83 proteins changed significantly between the pretreatment sample and the sample taken 7 weeks after the start of treatment. At 7 weeks after the start of treatment, 13 proteins showed lower expression in the CRT group compared to the RT group. The majority of the inflammatory proteins had returned to their pretreatment levels after 12 months. It was clearly demonstrated that cisplatin-based chemoradiation has immunological effects in patients with head and neck cancer. This analysis draws attention to several inflammatory proteins that are of interest for further studies.     

Modelling corn production in China using AVHRR‐based vegetation health indices

Weather‐related crop losses have always been a concern for farmers, governments, traders and policy makers for the purpose of balanced food supplies, demands, trade, and distribution of aid to nations in need. Therefore, early crop loss assessment in response to weather fluctuations is an important issue. This paper discusses the utility of Advanced Very High Resolution Radiometer (AVHRR)‐based vegetation health indices as a proxy for modelling corn yield and for early warning of drought‐related losses of agricultural production in China. The indices were tested in Jilin province on corn yield during 1982–2001 using correlation and regression analysis. A strong correlation between corn yield and the vegetation health indices were found during the critical period of corn growth, which starts 2–3 weeks before and 2–3 weeks after corn tassel. Following the results of correlation analysis, several regression equations were constructed where vegetation health indices were used as independent variables. The estimates of corn yield can be carried out well in advance of harvest and the errors of the estimates are 7–10%. The errors become smaller when the estimations are related to losses in corn yield due to drought.     

A case study in the mechanical verification of fault tolerance

To date, there is little evidence that modular reasoning about fault-tolerant systems can simplify the verification process in practice. This question is studied using a prominent example from the fault tolerance literature: the problem of reliable broadcast in point-to-point networks subject to crash failures of processes. The experiences from this case study show how modular specification techniques and rigorous proof re-use can indeed help in such undertakings.     

Materials Surgery – Reactivity Differences of Organic Groups in Hybrids

Synergistic properties in hybrid materials can emerge if the inorganic matrix has an electronic influence on the organic constituents and vice versa. This paper describes the drastic effect of SiO₂ in periodically ordered mesoporous organosilica materials (PMOs) on ethylene groups. A sophisticated, in situ solid‐state NMR spectroscopy study showed that the ozonolysis of ethylene groups follows an entirely different mechanism than is normal for organic, molecular groups. Ultimately, this leads to the topotactic transformation of the PMO material. Only if silicon is not in the alpha position to the double bond does it became possible to establish a new method to functionalize PMOs materials: the targeted scission of the ethylene group and the creation of functionalized pockets inside the pore walls of the mesoporous solid.     

Enhancing virtual environments with QoS aware resource management

Nowadays, the consolidation of application servers is the most common use for current virtualization solutions. Each application server takes the form of a virtual machine (VM) that can be hosted into one physical machine. In a default Xen implementation, the scheduler is configured to handle equally all of the VMs that run on a single machine. As a consequence, the scheduler shares equally all of the available physical CPU resources among the running VMs. However, when the applications that run in the VM dynamically change their resource requirements, a different solution is needed. Furthermore, if the resource usage is associated with service-level agreements, a predefined equal share of the processor power is insufficient for the VMs. Within the Xen’s primitives, even though it is possible to tune the scheduler parameters, there is no tool to achieve the dynamic change of the share of the processor power assigned to each VM. A combination of a number of primitives, however, appears to be suited as a base for achieving this. In this paper, we present an approach to efficiently manage the quality of service (QoS) of virtualized resources in multicore machines. We evaluate different alternatives within Xen for building an enhanced management of virtual CPU resources. We compare these alternatives in terms of performance, flexibility, and ease of use. We devise an architecture to build a high-level service that combines interdomain communication mechanisms with monitoring and control primitives for local resource management. We achieve this by our solution, a local resource manager (LRM), which adjusts the resources needed by each VM according to an agreed QoS. The LRM has been implemented as a prototype and deployed on Xen-virtualized machines. By means of experiments, we show that the implemented management component can meet the service-level objectives even under dynamic conditions by adapting the resources assigned to the virtualized machines according to demand. With the LRM, we therefore achieve both fine-grain resource allocation and efficient assignment.