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1.
Marialuisa Aragona Caterina Porcino Maria Cristina Guerrera Giuseppe Montalbano Rosaria Laur Marzio Cometa Maria Levanti Francesco Abbate Teresa Cobo Gabriel Capitelli Jos A. Vega Antonino German 《International journal of molecular sciences》2022,23(5)
The brain-derived neurotrophic factor (BDNF) was discovered in the last century, and identified as a member of the neurotrophin family. BDNF shares approximately 50% of its amino acid with other neurotrophins such as NGF, NT-3 and NT-4/5, and its linear amino acid sequences in zebrafish (Danio rerio) and human are 91% identical. BDNF functions can be mediated by two categories of receptors: p75NTR and Trk. Intriguingly, BDNF receptors were highly conserved in the process of evolution, as were the other NTs’ receptors. In this review, we update current knowledge about the distribution and functions of the BDNF-TrkB system in the sensory organs of zebrafish. In fish, particularly in zebrafish, the distribution and functions of BDNF and TrkB in the brain have been widely studied. Both components of the system, associated or segregated, are also present outside the central nervous system, especially in sensory organs including the inner ear, lateral line system, retina, taste buds and olfactory epithelium. 相似文献
2.
This work presents the formalization and the application of the factor separation technique in order to investigate the impact of precursor emission and their nonlinear interaction (in particulate matter accumulation processes). By processing the simulations of a 3D multiphase modeling system, the factor separation methodology can support the Environmental Authority in quantifying the impact of precursor emissions on PM10 production and consequently in assessing the feasible efficiency of different emission control strategies over a considered domain. The case study proposed by this paper focuses on the Po Valley region (Northern Italy), characterized by critical PM10 levels claiming for sound emission reduction policies. The results show the heavy nonlinearities and the strong seasonal dependence in the formation of PM10, over the study domain. Furthermore the results highlight that peak PM10 concentrations are mainly related to primary PM emissions in urban areas, and gas emissions (mainly NOx and NH3) in rural areas. 相似文献
3.
Sperm-mediated gene transfer in mice 总被引:1,自引:0,他引:1
OBJECTIVE: The purpose of this study was to perform a preliminary test of the hypothesis that patients infected with the human immunodeficiency virus (HIV) have an increased risk for serious odontogenic infections in comparison with HIV-negative patients. STUDY DESIGN: To address the research purpose, we used a case-control study design. A case was a serious odontogenic infection requiring inpatient management. A control was a nonserious odontogenic infection that could be managed on an outpatient basis. The ratio of controls to cases was 2:1. HIV status was determined by record review. RESULTS: The sample was composed of 300 patients. Sixteen patients (5%) were HIV-positive. Overall, 37.5% of the HIV-positive patients had serious infections; this compared with 33% of the HIV-negative patients (odds ratio = 1.21; 95% confidence interval = 0.43-3.44; P = .79). CONCLUSIONS: The results of this pilot study suggest that HIV-positive patients do not have an increased risk for developing serious odontogenic infections. 相似文献
4.
Cheese fortification using saturated monoglyceride self‐assembly structures as carrier of omega‐3 fatty acids 下载免费PDF全文
Sonia Calligaris Alexandra Ignat Marialuisa Biasutti Nadia Innocente Maria Cristina Nicoli 《International Journal of Food Science & Technology》2015,50(9):2129-2134
The purpose of this research was to study the capacity of emulsions containing saturated monoglyceride self‐assembly structures to deliver omega‐3 fatty acids in fresh soft cheese. To this aim, fortified emulsions containing different ratios of milk, saturated monoglycerides (MGs) and cod liver oil were added to milk before cheese‐making. These emulsions were characterised by distinct microstructural features observed by polarised light microscopy and apparent viscosity values. The omega‐3 delivery performance of MG emulsions highlighted that this strategy allowed a good retention of the omega‐3‐rich oil in the curd (up to 75%). The fortified cheeses showed yield value and fat content higher than those of control samples. The enriched cheese showed hardness and cohesiveness obtained by texture profile analysis similar to those of the unfortified product. Only a slight decrease in gumminess was detected in fortified cheese. 相似文献
5.
Anna Aiello Ernesto Fattorusso Marialuisa Menna Maurizio Pansini 《Advanced Synthesis \u0026amp; Catalysis》1995,337(1):397-400
A new carotenoid pigment ( 1 ) containing a diacetylenic function was isolated from a sample of Suberites massa collected in the Lagoon of Venice. Its structure and absolute configuration have been elucitated by spectroscopic method including 2D-NMR experiments. Also reported here is the analysis of the most abundant lipophylic metabolites of this organism. 相似文献
6.
Carla Zannella Annalisa Chianese Luciana Palomba Maria Elena Marcocci Rosa Bellavita Francesco Merlino Paolo Grieco Veronica Folliero Anna De Filippis Marialuisa Mangoni Lucia Nencioni Gianluigi Franci Massimiliano Galdiero 《International journal of molecular sciences》2022,23(4)
The COVID-19 pandemic has evidenced the urgent need for the discovery of broad-spectrum antiviral therapies that could be deployed in the case of future emergence of novel viral threats, as well as to back up current therapeutic options in the case of drug resistance development. Most current antivirals are directed to inhibit specific viruses since these therapeutic molecules are designed to act on a specific viral target with the objective of interfering with a precise step in the replication cycle. Therefore, antimicrobial peptides (AMPs) have been identified as promising antiviral agents that could help to overcome this limitation and provide compounds able to act on more than a single viral family. We evaluated the antiviral activity of an amphibian peptide known for its strong antimicrobial activity against both Gram-positive and Gram-negative bacteria, namely Temporin L (TL). Previous studies have revealed that TL is endowed with widespread antimicrobial activity and possesses marked haemolytic activity. Therefore, we analyzed TL and a previously identified TL derivative (Pro3, DLeu9 TL, where glutamine at position 3 is replaced with proline, and the D-Leucine enantiomer is present at position 9) as well as its analogs, for their activity against a wide panel of viruses comprising enveloped, naked, DNA and RNA viruses. We report significant inhibition activity against herpesviruses, paramyxoviruses, influenza virus and coronaviruses, including SARS-CoV-2. Moreover, we further modified our best candidate by lipidation and demonstrated a highly reduced cytotoxicity with improved antiviral effect. Our results show a potent and selective antiviral activity of TL peptides, indicating that the novel lipidated temporin-based antiviral agents could prove to be useful additions to current drugs in combatting rising drug resistance and epidemic/pandemic emergencies. 相似文献
7.
Annalisa Segat Marialuisa Biasutti Lucilla Iacumin Giuseppe Comi Federico Baruzzi Cristian Carboni Nadia Innocente 《LWT》2014
This work aimed to evaluate ozone effectiveness in reducing viable spoilage bacteria load throughout high moisture (HM) Mozzarella cheese-making process. At first, Mozzarella cheese samples were packaged with ozonated water (2 mg/L), stored at low temperature and monitored during shelf life. In a following phase cheese samples were put, before packaging, in direct contact with ozonated water at ozone concentrations of 2, 5 and 10 mg/L for 60 min. Then, gaseous ozone at concentrations of 10, 20 and 30 mg/m3 for different times was tested. In these experiments ozone was not effective in surface microbiological decontamination of cheeses. In all cases, there was no increase in the formation of primary and secondary lipid oxidation products. 相似文献
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9.
Assunta Virtuoso Roberto Giovannoni Ciro De Luca Francesca Gargano Michele Cerasuolo Nicola Maggio Marialuisa Lavitrano Michele Papa 《International journal of molecular sciences》2021,22(7)
Different functional states determine glioblastoma (GBM) heterogeneity. Brain cancer cells coexist with the glial cells in a functional syncytium based on a continuous metabolic rewiring. However, standard glioma therapies do not account for the effects of the glial cells within the tumor microenvironment. This may be a possible reason for the lack of improvements in patients with high-grade gliomas therapies. Cell metabolism and bioenergetic fitness depend on the availability of nutrients and interactions in the microenvironment. It is strictly related to the cell location in the tumor mass, proximity to blood vessels, biochemical gradients, and tumor evolution, underlying the influence of the context and the timeline in anti-tumor therapeutic approaches. Besides the cancer metabolic strategies, here we review the modifications found in the GBM-associated glia, focusing on morphological, molecular, and metabolic features. We propose to analyze the GBM metabolic rewiring processes from a systems biology perspective. We aim at defining the crosstalk between GBM and the glial cells as modules. The complex networking may be expressed by metabolic modules corresponding to the GBM growth and spreading phases. Variation in the oxidative phosphorylation (OXPHOS) rate and regulation appears to be the most important part of the metabolic and functional heterogeneity, correlating with glycolysis and response to hypoxia. Integrated metabolic modules along with molecular and morphological features could allow the identification of key factors for controlling the GBM-stroma metabolism in multi-targeted, time-dependent therapies. 相似文献
10.
The late blowing in cheese: a new molecular approach based on PCR and DGGE to study the microbial ecology of the alteration process 总被引:8,自引:0,他引:8
Cocolin L Innocente N Biasutti M Comi G 《International journal of food microbiology》2004,90(1):83-91
A molecular biology method based on polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) was developed to detect Clostridium spp. in cheese samples suspected of late blowing. Strains of Clostridium spp. and different Lactic Acid Bacteria species, obtained from international collections, were used to determine the experimental conditions for the PCR amplification and DGGE differentiation. DNA extracted directly from cheeses with late blowing symptoms was subjected to PCR and DGGE analysis and traditional agar plating was performed for samples pasteurized and enriched overnight. Moreover, volatile fatty acids were determined for comparison purposes. The PCR-DGGE results were in agreement with the plating performed, and only samples presenting DGGE bands migrating at the same position as Clostridium spp. bands, showed the presence of Clostridium colonies on Reinforced Clostridial Medium plates. Butyric acid contents were high (>100 mg/kg) in the cases of positive DGGE results, underlining the suitability of the protocol for the study of cheese spoilage. The sensitivity of the method is estimated to be 10(4) CFU/g. 相似文献