Peer-assisted learning interventions with elementary school students: A meta-analytic review.

A meta-analytic review of group comparison design studies evaluating peer-assisted learning (PAL) interventions with elementary school students produced positive effect sizes (ESs) indicating increases in achievement (unweighted mean ES = 0.59, SD = 0.90; weighted ES, d = 0.33, p     

Expectations and Experiences With MyLabourParty: From Right to Know to Right to Participate?

Social media are often argued to change political communication, decreasing the distance between politicians and citizens, and increasing citizens' influence on policymaking. This paper presents findings from a study of how the Norwegian Labour Party uses social media, focusing on the community platform MyLabourParty. Interviews with politicians and a survey with citizens as respondents were conducted to examine the experiences of online political discussions. Findings show that citizens and politicians expect MyLabourParty to strengthen citizen participation. Yet, citizens still experience communication asymmetry and politicians may find it challenging to participate to the extent citizens expect. Means for bridging the gap between expectations and experiences are called for, in particular redefinition of the offering, changes of user‐practices, and system improvements.     

A Short ERAP2 That Binds IRAP Is Expressed in Macrophages Independently of Gene Variation

The M1 zinc metalloproteases ERAP1, ERAP2, and IRAP play a role in HLA-I antigen presentation by refining the peptidome either in the ER (ERAP1 and ERAP2) or in the endosomes (IRAP). They have also been entrusted with other, although less defined, functions such as the regulation of the angiotensin system and blood pressure. In humans, ERAP1 and IRAP are commonly expressed. ERAP2 instead has evolved under balancing selection that maintains two haplotypes, one of which undergoing RNA splicing leading to nonsense-mediated decay and loss of protein. Hence, likewise in rodents, wherein the ERAP2 gene is missing, about a quarter of the human population does not express ERAP2. We report here that macrophages, but not monocytes or other mononuclear blood cells, express and secrete an ERAP2 shorter form independent of the haplotype. The generation of this “short” ERAP2 is due to an autocatalytic cleavage within a distinctive structural motif and requires an acidic micro-environment. Remarkably, ERAP2 “short” binds IRAP and the two molecules are co-expressed in the endosomes as well as in the cell membrane. Of note, the same phenomenon could be observed in some cancer cells. These data prompt us to reconsider the role of ERAP2, which might have been maintained in humans due to fulfilling a relevant function in its “short” form.     

Supplementation with SCFAs Re-Establishes Microbiota Composition and Attenuates Hyperalgesia and Pain in a Mouse Model of NTG-Induced Migraine

Migraine is a common brain-disorder that affects 15% of the population. Converging evidence shows that migraine is associated with gastrointestinal disorders. However, the mechanisms underlying the interaction between the gut and brain in patients with migraine are not clear. In this study, we evaluated the role of the short-chain fatty acids (SCFAs) as sodium propionate (SP) and sodium butyrate (SB) on microbiota profile and intestinal permeability in a mouse model of migraine induced by nitroglycerine (NTG). The mice were orally administered SB and SP at the dose of 10, 30 and 100 mg/kg, 5 min after NTG intraperitoneal injections. Behavioral tests were used to evaluate migraine-like pain. Histological and molecular analyses were performed on the intestine. The composition of the intestinal microbiota was extracted from frozen fecal samples and sequenced with an Illumina MiSeq System. Our results demonstrated that the SP and SB treatments attenuated hyperalgesia and pain following NTG injection. Moreover, SP and SB reduced histological damage in the intestine and restored intestinal permeability and the intestinal microbiota profile. These results provide corroborating evidence that SB and SP exert a protective effect on central sensitization induced by NTG through a modulation of intestinal microbiota, suggesting the potential application of SCFAs as novel supportive therapies for intestinal disfunction associated with migraine.     

Furan in the baby-food samples purchased from the Finnish markets – Determination with SPME–GC–MS

A method applying solid-phase microextraction followed by gas chromatography–mass spectrometric determination was in-house validated and used to study furan concentrations in baby-food samples purchased from the Finnish markets. The validation parameters showed that the method was well applicable for the reliable analysis of furan. Furan was analysed in 21 different baby-food samples as three independent replicates. The mean levels of furan varied between 4.7 and 90.3 μg kg⁻¹ being well in accordance with the levels reported in other studies. The mean concentrations of similar product formulas based on their ingredients were 9.2, 37.0 and 49.6 μg kg⁻¹ for fruit-, vegetables- and meat-containing baby-foods, respectively. According to the statistical analyses, fruit-based baby-food samples had significantly lower concentrations of furan as compared to other formulas. Based on our exercise, it seems that a low margin of safety exists between the extreme worst case infant exposures and the deduced NOAEL of furan on experimental animals, particularly for a clear rodent carcinogen.     

Identification of the Thermodynamically Stable Fe‐Containing Phase in Aged Cement Pastes

Current developments in cement chemistry increasingly rely on predictive thermodynamic modeling of the phase composition in cementitious composites with the aim of linking the performance of the material with the phase composition of the material. This approach requires identification of the cement phases that form in hydrating cementitious materials using standard techniques, such as X‐ray diffraction (XRD) and thermal analysis (DTA, TGA), but also state‐of‐the‐art synchrotron‐based techniques, in particular for those cases in which the signals of solid solutions overlap in XRD and TGA measurements. In this study, two ordinary Portland cements, with different chemical compositions and subject to different hydration times (~10, ~50 yr), were investigated aiming at identifying the most stable Fe‐containing cement phase in the cement pastes. The Fe‐containing cement phases and their solid solutions with the Al analogues in the complex cement matrix were analyzed with X‐ray absorption spectroscopy, indicating the formation of a mixed Fe–Al siliceous hydrogarnet as the major Fe‐containing phase. The presence of this phase after long hydration periods and upon selective dissolution of the pastes further indicates that, independent of the chemical compositions of cements, formation of the mixed Fe–Al siliceous hydrogarnet is thermodynamically favored in aged pastes, which is supported by published thermodynamic calculations.     

Chemical rescue of active site mutants of S. pneumoniae surface endonuclease EndA and other nucleases of the HNH family by imidazole

The His-Asn-His (HNH) motif characterizes the active sites of a large number of different nucleases such as homing endonucleases, restriction endonucleases, structure-specific nucleases and, in particular, nonspecific nucleases. Several biochemical studies have revealed an essential catalytic function for the first amino acid of this motif in HNH nucleases. This histidine residue was identified as the general base that activates a water molecule for a nucleophilic attack on the sugar phosphate backbone of nucleic acids. Replacement of histidine by an amino acid such as glycine or alanine, which lack the catalytically active imidazole side chain, leads to decreases of several orders of magnitude in the nucleolytic activities of members of this nuclease family. We were able, however, to restore the activity of HNH nuclease variants (i.e., EndA (Streptococcus pneumoniae), SmaNuc (Serratia marcescens) and NucA (Anabaena sp.)) that had been inactivated by His→Gly or His→Ala substitution by adding excess imidazole to the inactive enzymes in vitro. Imidazole clearly replaces the missing histidine side chain and thereby restores nucleolytic activity. Significantly, this chemical rescue could also be observed in vivo (Escherichia coli). The in vivo assay might be a promising starting point for the development of a high-throughput screening system for functional EndA inhibitors because, unlike the wild-type enzyme, the H160G and H160A variants of EndA can easily be produced in E. coli. A simple viability assay would allow inhibitors of EndA to be identified because these would counteract the toxicities of the chemically rescued EndA variants. Such inhibitors could be used to block the nucleolytic activity of EndA, which as a surface-exposed enzyme in its natural host destroys the DNA scaffolds of neutrophil extracellular traps (NETs) and thereby allows S. pneumoniae to escape the innate immune response.     

Decolorization of textile dyes by Bjerkandera sp. BOL 13 using waste biomass as carbon source

BACKGROUND: Phanerochaete chrysosporium, Trametes versicolor and Bjerkandera sp BOL13 were compared for decolorization of azo dyes supplied individually or as a mixture. The dye decolorization was also evaluated during continuous treatment under non‐sterile conditions using a lignocellulosic growth substrate. RESULTS: Bjerkandera sp BOL13 showed the highest dye decolorization potential. This fungus was also found to support high decolorization of Remazol Red RR at an initial pH of 4‐6 and when using straw as co‐substrate. The fungus was evaluated for Remazol Red RR decolorization in a continuously fed packed‐bed bioreactor operated under non‐sterile conditions with 3 days of hydraulic retention time. When glucose was supplied as growth‐substrate, decolorization efficiencies of 65‐90% were maintained for 12 days in a bioreactor packed with wooden material. The decolorization efficiency was lower when glucose was not fed to the fungus or when a plastic material was used as packing. Higher manganese peroxidase and laccase activities were also recorded when the wood packing was used. Contamination caused a drop in decolorization efficiency after 17‐19 days operation. CONCLUSIONS: The potential of Bjerkandera sp BOL13 for decolorization of azo dyes under non‐sterile conditions using lignocellulosic growth substrates was demonstrated. Research is needed to reduce contamination under non‐sterile conditions. © 2012 Society of Chemical Industry     

Effect of Silver Loading on the Lean NOx Reduction with Methanol Over Ag–Al2O3

The influence of silver loading on the lean NO_x reduction activity using methanol as reductant has been studied for alumina supported silver catalysts. In general, increasing the silver loading (0–3 wt%), in Ag–Al₂O₃, shifts or extends the activity window, for lean NO_x reduction towards lower temperatures. In particular Ag–Al₂O₃ with 3 wt% silver is active for NO_x reduction under methanol-SCR conditions in a broad temperature interval (200–500 °C), with high activity in the low temperature range (maximum around 300 °C) typical for exhaust gases from diesel and other lean burn engines. Furthermore, increasing the C/N molar ratio enhances the reduction of NO_x. However, too high C/N ratios results in poor selectivity to N₂.