The impact of roasting on the ochratoxin A content of coffee

Roasting coffee led to a drop in the ochratoxin A (OTA) concentration, as measured by the reference method, especially for dark type roasts. The way the beverage was prepared also affected the OTA content, which could paradoxically be higher than that of the initial roasted coffee. Assays on the thermal stability of pure OTA showed that it ought to be found in larger quantities in roasted coffee. This suggested that OTA was masked by reactions with the substrate during roasting. The absence of OTA in green coffee is therefore the best guarantee of safety.     

Mathematical modelling of the alcoholic fermentation of glucose by Zymomonas mobilis mobilis

The kinetics of alcoholic fermentation of a strain of Zymomonas mobilis, isolated from sugarcane juice, has been studied with the objective of determining the constansts of a non-structured mathematical model that represents the fermentation process. Assays in batch and in continuous culture have been carried out with different initial concentrations of glucose. The final concentrations of glucose, ethanol and biomass were determined. The following kinetic parameters were obtained: μ_max, 0·5 h^?1; K_s, 4·64 g dm^?3; P_max, 106 g dm^?3; Y_x/s, 0·0265 g g^?1; m, 1·4 g g^?1 h^?1; α, 17·38 g g^?1; β, 0·69 g g^?1 h^?1.     

Zeolites as catalysts in organic reactions. Claisen-Schmidt condensation of acetophenone with benzaldehyde

HY zeolites catalyze the crossed aldol condensation of acetophenone with benzaldehyde, in benzene at 80 °C, to give trans- and cis-chalcones. Together with these expected products, 3,3-diphenylpropiophenone is also produced. In the analogous basic condensation, using phase transfer catalysis, the Michael adduct was not detected, and besides chalcone a small percentage of the Cannizzaro reaction product was observed.     

Synthesis and Characterization of Al-MCM-41 and Al-MCM-48 Mesoporous Materials

A novel route in the synthesis of Al-MCM-41 and Al-MCM-48, using tetraethoxysilane (TEOS) and sodium aluminate (NaAlO₂) as Si and Al source has been obtained. The effect of surfactant nature and the synthesis conditions such as surfactant/Si ratio and hydrothermal treatment time on the formed mesostructure regularity has been studied. Different methods of template removal have also been evaluated. The samples were characterized by X-ray diffraction, nitrogen physisorption, FT-IR, and solid-state MAS NMR spectroscopy.     

Electron spin resonance studies of interactions of ammonia,copper, and cupriammonia with cellulose

The electron spin resonance spectra (ESR) of complexes of copper with fibrous cotton cellulose under various experimental conditions were determined. Cupric ions dissolved in solutions of strong bases, such as concentrated ammonium hydroxide, sodium hydroxide, and potassium hydroxide, formed complexes with fibrous cotton cellulose. These complexes had paramagnetic resonance properties and generated characteristic ESR spectra. Cupric ions dissolved in solutions of highly ionized neutral salts, such as sodium chloride, formed complexes with cellulose. These complexes also generated the same characteristic ESR spectra as the complexes formed in solutions of strong base. The reaction between cupric ions and cellulose was evidently very rapid and reversible. When the concentration of ammonia was decreased in, or ammonia was removed from, the cupric ion–ammonium hydroxide–cellulose complexes, the paramagnetic resonance properties of the complex were decreased or lost. Similar results were received when potassium hydroxide was removed from the complexes. The compositions of the complexes evidently are variable, that is, under different experimental conditions the relative intensities of the lines of the ESR spectra of the complexes varied, although the hyperfine splittings of the lines were constant. It was concluded that reactions of cupric ions to form complexes with adjacent hydroxyl groups on the cellulose molecule depended on an optimum spatial arrangement of the hydroxyl groups, that is, distance between the groups. Evidently, wetting of cotton cellulosic fibers with solutions of strong bases or neutral salt allowed rotation about the C₂–C₃ bond to yield this optimum arrangement. When the base or salt was removed, rotation occurred to give less favorable positions of the hydroxyl groups for complexing with cupric ions.     

An experimental study on the applicability of evolutionary algorithms to craniofacial superimposition in forensic identification

Photographic supra-projection is a forensic process that aims to identify a missing person from a photograph and a skull found. One of the crucial tasks throughout all this process is the craniofacial superimposition which tries to find a good fit between a 3D model of the skull and the 2D photo of the face. This photographic supra-projection stage is usually carried out manually by forensic anthropologists. It is thus very time consuming and presents several difficulties. In this paper, we aim to demonstrate that real-coded evolutionary algorithms are suitable approaches to tackle craniofacial superimposition. To do so, we first formulate this complex task in forensic identification as a numerical optimization problem. Then, we adapt three different evolutionary algorithms to solve it: two variants of a real-coded genetic algorithm and the state of the art evolution strategy CMA-ES. We also consider an existing binary-coded genetic algorithm as a baseline. Results on several superimposition problems of real-world identification cases solved by the Physical Anthropology lab at the University of Granada (Spain) are considered to test our proposals.     

Using OGRO and CertiVeR to improve OCSP validation for Grids

Authentication and authorization in many distributed systems rely on the use of cryptographic credentials that in most of the cases have a defined lifetime. This feature mandates the use of mechanisms able to determine whether a particular credential can be trusted at a given moment. This process is commonly named validation. Among available validation mechanisms, the Online Certificate Status Protocol (OCSP) stands out due to its ability to carry near real time certificate status information. Despite its importance for security, OCSP faces considerable challenges in the computational Grid (i.e. Proxy Certificate’s validation) that are being studied at the Global Grid Forum’s CA Operations Work Group (CAOPS-WG). As members of this group, we have implemented an OCSP validation infrastructure for the Globus Toolkit 4, composed of the CertiVeR Validation Service and our Open GRid Ocsp (OGRO) client library, which introduced the Grid Validation Policy. This paper summarizes our experiences on that work and the results obtained up to now. Furthermore we introduce the prevalidation concept, a mechanism analogous to the Authorization Push-Model, capable of improving OCSP validation performance in Grids. This paper also reports the results obtained with OGRO’s prevalidation rules for Grid Services as a proof of concept.

Inflating examples to obtain rules

A new machine learning system is presented in this article. It is called INNER and induces classification rules from a set of training examples. The process followed by this system starts with the random selection of a subset of examples that are iteratively inflated in order to cover the surroundings provided that they are inhabited by examples of the same class, thus becoming rules that will be applied by means of a partial matching mechanism. The rules so obtained can be seen as clusters of examples and represent clear evidence to support explanations about their future classifications and may be used to build intelligent advisors. The whole algorithm can be seen as a set of elastic transformations of examples and rules and produces concise, accurate rule sets, as is experimentally demonstrated in the final section of the article. © 2003 Wiley Periodicals, Inc.