Rapid Combinatorial Synthesis and Chromatography Based Screening of Phosphorescent Iridium Complexes for Solution Processing

This work reports the combinatorial synthesis and screening of phosphorescent iridium complexes as solution processable emitters for OLEDs. The approach taken here allows for the rapid synthesis, isolation, spectroscopic characterization and identification of the libraries based on chromatographic methods. Subsequent analysis of the irradiation induced degradation provides insight on the stability of the complexes under continuous excitation. The method is versatile and can easily be applied to other metal complexes or organic dyes for various applications, e.g., in electroluminescence, photovoltaics and sensing.     

Interfacing Renewable Energy Sources to the Utility Grid Using a Three-Level Inverter

This paper presents a novel approach for the connection of renewable energy sources to the utility grid. Due to the increasing power capability of the available generation systems, a three-level three-phase neutral-point-clamped voltage-source inverter is selected as the heart of the interfacing system. A multivariable control law is used for the regulator because of the intrinsic multivariable structure of the system. A current source (playing the role of a generic renewable energy source) is connected to the grid using a three-level inverter in order to verify the good performance of the proposed approach. Large- and small-signal d-q state-space averaged models of the system are obtained and used to calculate the multivariable controller based on the linear quadratic regulator technique. This controller simultaneously regulates the dc-link voltage (to operate at the maximum power point of the renewable energy source), the mains power factor (the power is delivered to the grid at unity power factor), and the dc-link neutral-point voltage balance. With the model and regulator presented, a specific switching strategy to control the dc-link neutral-point voltage is not required. The proposed controller can be used for any application, since its nature makes possible the control of any system variable. The good performance of the presented interfacing solution in both steady-state and transient operation is verified through simulation and experimentation using a 1-kW neutral-point-clamped voltage-source-inverter prototype, where a PC-embedded digital signal processor board is used for the controller implementation     

Conducted EMI Reduction in Power Converters by Means of Periodic Switching Frequency Modulation

Spread spectrum clock generation techniques were originally developed to reduce electromagnetic interference (EMI) in communications and microprocessor systems working in the range of hundreds of megahertz. Nowadays, the switching frequency of power converters has been increasing up to values that make worthy the application of such switching frequency modulation techniques to reduce EMI emissions in power converters. Although random modulations have been applied before to power converters, periodic patterns can provide some advantages. First, theoretical principles of frequency modulation using three periodic patterns for the modulating function are presented. The influence of some important modulation parameters on the EMI reduction is analyzed and some considerations about the EMI filters design are also presented. The effectiveness of such methods in terms of EMI reduction is demonstrated theoretically and confirmed with experimental results obtained from tests carried out on two converters. The first one is a 2.5 W buck converter that can be switched up to 1 MHz and the second one is a 600 W boost converter switching at 40 kHz. In both cases, attenuations obtained in conducted EMI are evaluated. Finally, special attention has been paid to input current and output voltage ripple in order to evaluate possible undesired side-effects produced by this technique.     

The trend of reduced hop‐content in Pilsner‐type beer in Germany. A change in taste?

A time series analysis of the development of bitterness units (BU) of a collective of 1,202 Pilsner beer samples analysed between 1983 and 2013 shows a small but statistically significant decline from values of around 30 BU in the 1980s to values of around 27 in the last years. The results confirm a trend to lower hopped Pilsner beers, which could derive from economic pressures on the breweries combined with a lack of regulations, or a change in consumer preference. So–called Pilsner beers with extremely low BU values are judged as a being misleading to the consumer, which is an offence against European food law. Copyright © 2015 The Institute of Brewing & Distilling     

Acrylamide levels in Finnish foodstuffs analysed with liquid chromatography tandem mass spectrometry

Sample clean-up and HPLC with tandem mass spectrometric detection (LC-MS/MS) was validated for the routine analysis of acrylamide in various foodstuffs. The method used proved to be reliable and the detection limit for routine monitoring was sensitive enough for foods and drinks (38 microg/kg for foods and 5 microg/L for drinks). The RSDs for repeatability and day-to-day variation were below 15% in all food matrices. Two hundred and one samples which included more than 30 different types of food and foods manufactured and prepared in various ways were analysed. The main types of food analysed were potato and cereal-based foods, processed foods (pizza, minced beef meat, meat balls, chicken nuggets, potato-ham casserole and fried bacon) and coffee. Acrylamide was detected at levels, ranging from nondetectable to 1480 microg/kg level in solid food, with crisp bread exhibiting the highest levels. In drinks, the highest value (29 microg/L) was found in regular coffee drinks.     

Specificity grafting of human antibody frameworks selected from a phage display library: generation of a highly stable humanized anti-CD22 single-chain Fv fragment

A prerequisite for the enrichment of antibodies screened fromphage display libraries is their stable expression on a phageduring multiple selection rounds. Thus, if stringent panningprocedures are employed, selection is simultaneously drivenby antigen affinity, stability and solubility. To take advantageof robust pre-selected scaffolds of such molecules, we graftedsingle-chain Fv (scFv) antibodies, previously isolated froma human phage display library after multiple rounds of in vitropanning on tumor cells, with the specificity of the clinicallyestablished murine monoclonal anti-CD22 antibody RFB4. We showthat a panel of grafted scFvs retained the specificity of themurine monoclonal antibody, bound to the target antigen withhigh affinity (6.4–9.6 nM), and exhibited exceptionalbiophysical stability with retention of 89–93% of theinitial binding activity after 6 days of incubation in humanserum at 37°C. Selection of stable human scaffolds withhigh sequence identity to both the human germline and the rodentframeworks required only a small number of murine residues tobe retained within the human frameworks in order to maintainthe structural integrity of the antigen binding site. We expectthis approach may be applicable for the rapid generation ofhighly stable humanized antibodies with low immunogenic potential. Received June 10, 2003; accepted August 27, 2003.     

Chlorate Formation in Chlorine Dioxide Delignification—An Analysis via Elementary Kinetic Modeling

Abstract

Elementary kinetic modeling was used to study the mechanism of chlorate formation in chlorine dioxide delignification. Reaction conditions reflecting typical industrial processes (T = 50°C, pH 1.5–4) were examined. Fe mediated Cl(III) decomposition and a reaction between hypochlorous acid and chlorous acid (or their equilibrium counterparts) were found to be the major reaction routes responsible for chlorate formation at pH < 3. The latter route accounts for chlorate formation at pH ≥ 3. The rate of chlorous acid (HClO₂) self-decomposition was too slow either to compete against the other routes (pH < 3) or to yield notable amounts of chlorate within the given time frame (pH ≥ 3). The results suggest that chlorate formation could be suppressed, without adverse effects on chlorine dioxide regeneration, by aiming for end pH 3–3.5, ensuring a moderate chloride ion concentration and by favoring concentrated solutions/suspensions.