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1.
Anna Bertova Szilvia Kontar Zoltan Polozsanyi Martin Simkovic Zuzana Rosenbergova Martin Rebros Zdena Sulova Albert Breier Denisa Imrichova 《International journal of molecular sciences》2022,23(18)
The expression of the membrane ABCB1 transporter in neoplastic cells is one of the most common causes of reduced sensitivity to chemotherapy. In our previous study, we investigated the effect of a single culture of ABCB1-negative (S) and ABCB1-positive variants of L1210 cells (R and T) in the presence of sulforaphane (SFN). We demonstrated that SFN induces the onset of autophagy more markedly in S cells than in R or T cells. In the current study, we focused on the effect of the repeated culture of S, R and T cells in SFN-containing media. The repeated cultures increased the onset of autophagy compared to the simple culture, mainly in S cells and to a lesser extent in R and T cells, as indicated by changes in the cellular content of 16 and 18 kDa fragments of LC3B protein or changes in the specific staining of cells with monodansylcadaverine. We conclude that SFN affects ABCB1-negative S cells more than ABCB1-positive R and T cells during repeated culturing. Changes in cell sensitivity to SFN appear to be related to the expression of genes for cell-cycle checkpoints, such as cyclins and cyclin-dependent kinases. 相似文献
2.
By a special plastometric method, the straightening operation of a vertically cast strand of two carbon steels was simulated. The temperature dependence of the low strain rate plasticity of both the steels examined is analogous although caused by very different reasons. As to the plain carbon steel, the amount of ferrite and its location is of prime importance, whereas in the case of eutectoid steel various modes of fracture and the influence of temperature on the relative plasticity of type II MnS inclusions are influencial. To avoid transverse cracking, it seems to be suitable to keep the strand at a rather high temperature (above 900 °C). Temperature cycling seems to be fundamental as to the structural changes taking place in the strand (grain size refining, ferrite fraction if any), whereas analogous influences of straining are more or less inexpressive. Of course, the results obtained are of quality only since the practical and laboratory conditions differ significantly. The knowledge obtained could be applied in regulating the intensity of strand cooling with the aim of lowering the waste portion and improving the surface quality of worked products. 相似文献
3.
Usually in islands both primary energy sources and drinking water are missing. Additionally, municipal solid waste (MSW) must be managed avoiding exclusive use of landfills, which limits sustainable development. Power generation from MSW incineration contributes significantly to replacing energy produced from fossil fuels and to reduce overall emissions. A solution based on thermodynamics, environmental and economic analyses and 3D-GIS modelling for the afore-mentioned problems for Cape Verde is proposed. This model integrates waste transportation optimisation and incineration with energy recovery combining production of heat and power (CHP), the heat being used for drinking water production. The results show that extraction condensing steam turbines are more suitable when power production is a priority (5.0 MW with 4000 m3/d of drinking water), whereas back-pressure turbines yield 5540–6650 m3/d of drinking water with an additional power production of 3.3–4.7 MW. The environmental and economic assessment performed shows the feasibility of the proposed CHP solution, which brings a considerable reduction in net air emissions (1.6 kt), including a significant decrease in the greenhouse gas emissions (131 ktCO2), and that the revenue from energy sales (€15 million) has potential to balance the incineration cost. Moreover, when terrain relief is accounted for in the route optimisation for minimum fuel consumption, savings up to 11% are obtained. 相似文献
4.
It was demonstrated that non-homopolymerizing derivatives of 6-caprolactam: 7-cyclohexyl-1-aza-2-cycloheptanone (I) and 7-isopropyl-5-methyl-1-aza-2-cycloheptanone (II) were polymerizing with 6-caprolactam under conditions of the socalled hydrolytic polymerization. With its increasing content in the initial reaction mixture the copolymerization rate, the equilibrium content of the copolymer, and the reduced viscosity decreased. Lactam (I) was a more reactive comonomer in comparison with lactam (II). 相似文献
5.
Dynamic mechanical analysis is performed on polybenzoxazines from acetylene-terminated benzoxazine monomers, and glass transition temperatures of these polybenzoxazines are found in the range of 329–368°C. It has been identified that the high glass transition temperature and high thermal stability are due to polymerization of the acetylene terminal group, in addition to oxazine ring polymerization through a comparison study with analogous polybenzoxazines that are obtained from monomers without an acetylene functional group. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 857–862, 1999 相似文献
6.
David Segorbe Derek Wilkinson Alexandru Mizeranschi Timothy Hughes Ragnhild Aaløkken Libuše Váchová Zdena Palková Gregor D. Gilfillan 《Yeast (Chichester, England)》2018,35(8):507-512
We report an optimized low‐input FAIRE‐seq (Formaldehyde‐Assisted Isolation of Regulatory Elements‐sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few colonies. Sensitivity, specificity and reproducibility of the scaled‐down method are comparable with those of regular, higher input amounts, and allow the use of 100‐fold fewer cells than existing procedures. The method enables epigenetic analysis of chromatin structure without the need for cell multiplication of exponentially growing cells in liquid culture, thus opening the possibility of studying colony cell subpopulations, or those that can be isolated directly from environmental samples. 相似文献
7.
Dudás A Marková E Vlasáková D Kolman A Bartosová Z Brozmanová J Chovanec M 《Yeast (Chichester, England)》2003,20(5):389-396
The Saccharomyces cerevisiae rad52 mutants are sensitive to many DNA damaging agents, mainly to those that induce DNA double-strand breaks (DSBs). In the yeast, DSBs are repaired primarily by homologous recombination (HR). Since almost all HR events are significantly reduced in the rad52 mutant cells, the Rad52 protein is believed to be a key component of HR in S. cerevisiae. Similarly to the S. cerevisiae Rad52 protein, RecA is the main HR protein in Escherichia coli. To address the question of whether the E. coli RecA protein can rescue HR defective phenotype of the rad52 mutants of S. cerevisiae, the recA gene was introduced into the wild-type and rad52 mutant cells. Cell survival and DSBs induction and repair were studied in the RecA-expressing wild-type and rad52 mutant cells after exposure to ionizing radiation (IR) and methyl methanesulphonate (MMS). Here, we show that expression of the E. coli RecA protein partially complemented sensitivity and fully complemented DSB repair defect of the rad52 mutant cells after exposure to IR and MMS. We suggest that in the absence of Rad52, when all endogenous HR mechanisms are knocked out in S. cerevisiae, the heterologous E. coli RecA protein itself presumably takes over the broken DNA. 相似文献
8.
Zdena Tvarůžková Magda Tupá Pavel Jírů Alfonso Nastro Giorgio Giordano Ferruccio Trifiró 《Catalysis Letters》1989,2(6):369-374
The small pore zeolite HLZ 132 exhibits, in comparison with other zeolites, an increased selectivity for the transformation of methanol to ethylene in the reaction temperature range 350–500 °C: the weight ratio of C2H4 to C3H6 in the products ranges between 1 and 4 at WHSV=2 h–1. Besides the effect of the reactant shape selectivity this fact may be interpreted by the participation of the asymmetrical methoxy groups in the surface as well as by proton-donor centres of lower acidity which do not catalyze the oligomerization of ethylene but which do the more basic molecule of propylene, thereby generating polyene-type coke. 相似文献
9.
Tatiana Bubencíkova Dana Cholujová Lucia Messingerová Danica Mislovicova Mario Seres Albert Breier Zdena Sulova 《International journal of molecular sciences》2012,13(11):15177-15192
P-glycoprotein (P-gp) overexpression is the most frequently observed cause of multidrug resistance in neoplastic cells. In our experiments, P-gp was expressed in L1210 mice leukemia cells (S cells) by selection with vincristine (R cells) or transfection with the gene encoding human P-gp (T cells). Remodeling of cell surface sugars is associated with P-gp expression in L1210 cells as a secondary cellular response. In this study, we monitored the alteration of cell surface saccharides by Sambucus nigra agglutinin (SNA), wheat germ agglutinin (WGA) and Maackia amurensis agglutinin (MAA). Sialic acid is predominantly linked to the surface of S, R and T cells via α-2,6 branched sugars that tightly bind SNA. The presence of sialic acid linked to the cell surface via α-2,3 branched sugars was negligible, and the binding of MAA (recognizing this branch) was much less pronounced than SNA. WGA induced greater cell death than SNA, which was bound to the cell surface and agglutinated all three L1210 cell-variants more effectively than WGA. Thus, the ability of lectins to induce cell death did not correlate with their binding efficiency and agglutination potency. Compared to S cells, P-gp positive R and T cells contain a higher amount of N-acetyl-glucosamine on their cell surface, which is associated with improved WGA binding. Both P-gp positive variants of L1210 cells are strongly resistant to vincristine as P-gp prototypical drug. This resistance could not be altered by liberalization of terminal sialyl residues from the cell surface by sialidase. 相似文献
10.
Jana Nováková Michael Schwarze Zdena Tvarůžková Zdeněk Sobalík 《Catalysis Letters》2004,98(2-3):123-127
The dehydration of Fe-ferrierites doubled the decomposition of N2O at 200 ° C and corresponding deposition of oxygen. The rehydration resulted in a reversible process. Samples with iron located predominantly in cationic positions (Fe/Al 0.05–0.25) exhibited the O dep /Fe ratio of ca. 0.5 and 1 after pretreatment at 450 and 700 °C, respectively. 相似文献