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1.
The UvrA and UvrB proteins form part of the UvrABc endonuclease, which is responsible for nucleotide excision repair in Escherichia coli. Using a mobility shift gel assay we have studied the binding of UvrA dimer, UvrB monomer and UvA(2)B trimer complexes with 40, 50 and 136 bp (32)P-end-labelled DNA fragments adducted with aflatoxin B(1). UvrA was shown to re-associate with adduct specific UvrB: DNA complexes, a phenomenon which could be reversed by the addition of 500 mM potassium chloride or anti-UvrA anti-sera. Re-association was shown to be UvrA concentration dependent. Re-association of UvrA(2)B to the UvrB:DNA complex was not seen. We have also shown that the UvrB:DNA complex, in the case of aflatoxin B(1), is extremely stable with a half-life excess of 400 min and that fragment termini are not a specific substrate for UvrA binding. 相似文献
2.
Jaspreet S. Gandhi William J. van Ooij 《Journal of Materials Engineering and Performance》2004,13(4):475-480
Organofunctional silanes recently have emerged as outstanding, environmentally friendly corrosion protectors for metal substrates,
compared with conventional chromate treatments. A simple immersion technique is typically used to coat the metal surface with
silane films. However, the thickness and uniformity of the films are uncontrolled in this process. This paper proposes a new
deposition technique for the silane films on the metal surface, i.e., by electrodeposition. Hydrolyzed silanes are water-soluble,
ionized molecules, so they can be deposited on metals by electrodeposition. Various combinations of silane mixtures were tested
at different voltages, pH values, bath concentrations, and exposure times on panels of alloy aluminum and mirror-polished
ferro-plate. The surface structure was characterized by scanning electron microscopy (SEM) and ellipsometry. The resistance
of the film to corrosion was investigated by direct current (DC) polarization and electrochemical impedance spectroscopy (EIS)
techniques. Electrodeposition results in a more organized and uniform film with fewer pores, compared with immersed or dipped
films.
This paper was presented at the 2nd International Surface Engineering Congress sponsored by ASM International, on September
15–17, 2003, in Indianapolis, Indiana, and appears on pp. 320–26 of the Proceedings. 相似文献
3.
4.
Pd supported on KL zeolite has been studied by temperature programmed reduction (TPR) and electron paramagnetic resonance (EPR) spectroscopy. It has been found that upon calcination in pure oxygen Pd3+ and Pd2+ ions are formed; the Pd3+ ions are identified by their EPR signal. As Pd3+ ions in the cancrinite cages are difficult to reduce, the H2 consumption in conventional TPR is below the stoichiometric amount. 相似文献
5.
Experiments using a parallel plate waveguide as the measurement medium have revealed a strong dependence of RF absorption in rats and biological-phantom prolate spheroidal bodies upon the frequency and polarization of electromagnetic fields. The results correlate well to those of free space irradiation. Strongest power deposition is observed for fields polarized along the long dimension of the bodies for frequencies such that the major length is about one-quarter wavelength of radiation. At resonance, an effective absorption area of 2.5 to 3.5 times the shadow cross section is measured. 相似文献
6.
V. N. Aju kumar O. P. Gandhi 《Quality and Reliability Engineering International》2011,27(8):1145-1172
Assessment of human error in maintenance requires identification of the contributing factors that lead to human error(s). These factors are called human error inducing factors (HEIFs), which take into consideration both the active and latent error contributing aspects related to man, machine and environment. A systems approach of the Graph Theory is applied in this paper for quantifying human error in maintenance activities that models the identified factors and their interactions/interrelationships in terms of human error digraph. The nodes in the digraph represent the HEIFs and the edges represent their interrelationships. The digraph is converted into an equivalent matrix and an expression based on this is developed, which is characteristic of the human error in maintenance. This expression is used to evaluate a human error index by substituting the numerical value of the factors and their interrelations. The index is a measure of the human error potential involved in the maintenance of systems. A higher value of index indicates that the error likelihood is more for the associated tasks, and more efforts are required to make the system less prone to human error. The proposed methodology is illustrated using a case study. The approach is anticipated to play a significant role in identifying sources of human errors and predicting their impact; and will help to integrate human factors during design stage with the objective of reducing human error in maintenance. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
7.
Numerical simulation of annular-phased arrays of dipoles forhyperthermia of deep-seated tumors 总被引:1,自引:0,他引:1
We have used the finite-difference time domain (FDTD) method to calculate the SAR distributions from an annular-phased array of eight dipole antennas coupled through water "boluses" in anatomically based three-dimensional models of the human body. We evaluated the effect of tapered bolus chambers, frequency (100-120 MHz), dipole length (17-30 cm), and phase and amplitude of power to the various dipoles on the ability to focus energy in the region of deep-seated tumors in the prostate and the liver. Assuming tumor conductivity and permittivity to be similar or slightly higher than surrounding normal tissues, calculations indicate that adjustment of the noted parameters should result in considerable improvement in focusing of SAR distributions in tumor-bearing regions. If such calculations can be shown to correctly predict empirical measurements from complex inhomogeneous (although not necessarily anatomically correct) phantoms, they may be useful for hyperthermia treatment planning based on patient-specific anatomic models. 相似文献
8.
The surfaces of Pt-45Pd-10Rh foils oxidized over the range 875–1075 K in a 20% O2-Ar mixture at atmospheric pressure were examined by Auger electron, X-ray photoelectron, and Raman spectroscopy. The composition of the oxide formed on the surface was found to vary with temperature from predominantly PdO at 875 K to PdRhO2 at 1075 K. Only a few atomic percent Pt was observed, present in both the metallic and (apparently) +1 oxidation states at 875 K and in the metallic state at 1075 K. The formation of PdRhO2 (and no Rh2O3) at 1075 K was found to persist upon reoxidation following a low-temperature reduction cycle in which the increased Rh concentration on the surface was retained. An oxidation-induced Rh enrichment of the surface of the alloy foil beyond 50 at. % does not appear likely within the temperature/pressure regime investigated. 相似文献
9.
Gaurav Gandhi 《Analog Integrated Circuits and Signal Processing》2006,46(2):173-178
An improved implementation of Chua's circuit using dual output current conveyor is presented. The present proposal offers
the advantages of low component count, current mode processing, availability of current across inductor and availability of
voltage across inductor in form of current. The application of present circuit in designing low hardware inductorless hyperchaotic
circuit is also presented. The paper concludes with a discussion of advantages of present proposal of Chua's circuit and inductorless
hyper chaotic circuit thus designed over their earlier counterparts. The designs are adequately supported by SPICE simulation
results.
Patent pending. 相似文献
10.
An analytical method to predict and optimize the performance of Berenger's (see J. Computat. Phys., vol.114, p.185-200, 1994) perfectly matched layer (PML) absorbing boundary condition (ABC) for finite-difference time-domain (FDTD) simulations is described. The shape of the conductivity in the PML layers has to be chosen carefully to obtain the best performance for a given number of layers. The relative error is shown to be the composite of two distinct effects: the theoretical reflection coefficient given by the PML layers backed by a metal plane and the second-order error in the differential intrinsic in the FDTD formulation. A theoretical expression to evaluate this error as a function of the number of PML layers and the shape of the conductivity is given, and the result is compared to that obtained for several FDTD test cases. The good agreement of the shapes of the theoretical and numerically derived curves allows the use of the theoretical formulation to optimize the PML region as a function of the shape of the conductivity, resolution, and number of layers 相似文献