Immobilization Horseradish Peroxidase onto UiO-66-NH2 for Biodegradation of Organic Dyes

Enzymes are extensively used as catalyst in several fields of production such as chemistry, and pharmaceuticals owing to their selectivity, efficiency and environmentally friendliness. However, their applications are often hindered due to their insufficient stability and difficulties in re-use. As a member of porous crystalline materials, metal organic frameworks are a promising enzyme carrier due to their multi-functional pore surfaces and robustness in variety of harsh conditions. In this study, the horseradish peroxidase (HRP) enzyme was immobilized onto UiO-66-NH₂ (Universitetet i Oslo) by a facile incubation method at the room temperature to improve the stability and reusability of enzyme. The prepared HRP@UiO-66-NH₂ bio-composite was characterized by using FT-IR, XRD and SEM. The crystal structure of MOF was well-preserved after enzyme immobilization. A colorimetric assay for enzyme activity after released from UiO-66-NH₂ has been employed based on the catalytic oxidation of phenol coupled with 4-aminoantipyrine. The robustness and activity of immobilized enzyme after released from UiO-66-NH₂ were investigated by biodegradation of methyl orange (MO) and methylene blue (MB) with several parameters such as pH, temperature, the dosage of H₂O₂ and the dye concentration with comparison to its free form. The optimum condition for dye degradation was obtained at basic conditions. The immobilized enzyme maintained its activity at elevated temperature while free enzyme lost its activity at the same conditions, attributed to the armoring effect of UiO-66-NH₂. According to the results of studied various parameters, MO and MB were biodegraded to 60% and 45%, respectively, within 60 min with the optimum conditions at pH 9 and 50 °C at a H₂O₂ dosage of 3%. The superior pH tolerance and stability suggest potential of UiO-66-NH₂ immobilized peroxidase enzyme in industrial applications.

     

Robust microencapsulated phase change materials in concrete mixes for sustainable buildings

For passive building applications, phase change materials (PCMs) are microencapsulated to avoid leakage of PCM from concrete structure. The primary challenge of using microencapsulated PCM (MPCM) is its weak shell structure. New MPCMs with different shell compositions to prevent breakage during mixing in fresh concrete are needed. In this study, free radical polymerization method to microencapsulate capric acid–myristic acid mixture as PCM with two different methyl methacrylate co‐polymers is proposed to produce robust MPCMs for building applications. Two new microcapsules (MPCM‐1 and MPCM‐2) having latent heats of 91.9 and 97.3 J/g were synthesized. SEM analyses showed the size of microcapsules being in the range of 400–850 nm for MPCM‐1 and 250–475 nm for MPCM‐2. Analyses also reveal that the shells of MPCMs were not harmed, as they were added into concrete mixes. The microsphere's geometry was preserved, and distribution was homogeneous. The MPCMs were also studied under thermal tests of 1000 heating/cooling cycles. No significant changes in thermal properties were observed after thermal cycling tests. Copyright © 2016 John Wiley & Sons, Ltd.     

The effect of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation in the splenectomized rat

BACKGROUND/AIMS: We created a study group in order to investigate the effects of splenectomy and filgrastim. Filgrastim is an immunomodulator granulocyte colony-stimulating factor (G-CSF), that affects bacterial translocation. MATERIALS AND METHODS: We created 3 study groups with 30 male Sprague-Dawley rats; the first group included sham splenectomy, the second group was splenectomy, and the third group was splenectomy+ filgrastim group. RESULTS: The mean bacterial colony count of the cecum were 2.5 x 10(9) in group 1, 1.2 x 10(10) in group 2 and 3.5 x 10(9) in group 3. The differences between these groups were accepted as statistically significant. The mean counts of the terminal ileum were 1.1 x 10(9) in group 1, 5.5 x 10(10) in group 2 and 2.5 x 10(10) in group 3. The p values of group 1-2 were 0.036 (statistically significant) and 0.123 in groups 2-3) were not statistically significant. The mean counts of the liver were 0.2 x 10(4), 1 x 10(10) and 3.4 x 10(5), respectively. In comparison of the groups the p values of the first and last 2 groups were found to be 0.047 (statistically significant). The mean counts of the mesenteric lymph node were 0.7 x 10(3), 1 x 10(10) and 0.9 x 10(6) respectively. The p values were 0.343 for the first and the last 2 groups both. As they were above 0.05, they were not statistically significant. The degrees of liver Kupffer cell hyperplasia were (+) 40%, (+2) 50% and (+3) 10% (group 1), (+) 10%, (+2) 40% and (+3) 50% (group 2), (+2) 60% and (+3) 40% (group 3) (p = 0.0039). The rates of the absence of pathology in mesenteric lymph nodes were 70% (group 1), 90% (group 2) and 100% (group 3) (p = 0.049). These findings were statistically significant. CONCLUSIONS: We found that splenectomy has activated the whole predisposing factors of bacterial translocation and created the latter itself. In addition, we showed that filgrastim, a recently widespread used G-CSF, decreases bacterial translocation significantly.     

Developing microencapsulated 12‐hydroxystearic acid (HSA) for phase change material use

Phase change materials (PCM) have an increasingly more important role as a thermal energy storage (TES) media. However, leakage problem of PCM causes limitation during their integration in TES systems. Therefore, the encapsulation of PCMs is attracting research interest to extend usage of PCMs in real TES applications in recent years. In this study, hydroxystearic acid (HSA) was encapsulated with polymethyl methacrylate (PMMA) and different PMMA comonomer shells via emulsion polymerization method for the first time in literature. HSA with high melting temperature range (74–78°C) can widen the scope of using PCMs, and the encapsulated form can make it more versatile. The chemical structures, morphologies, and thermophysical properties of capsules were determined by FT‐IR, SEM, DSC, TGA, and thermal infrared camera. Among the produced HSA capsule candidates, PMMA‐HEMA is the most promising with latent heat of 48.5 J/g with melting range of 47 to 85°C. SEM analysis indicated that the capsules have spherical shape with compact surface at nano‐micro (100–440 nm) size range; however, some capsules exhibited agglomeration.     

Laboratory investigation on the use of thermally enhanced phase change material to improve the performance of borehole heat exchangers for ground source heat pumps

Ground source heat pumps have high efficiency and high capital cost primarily due to borehole drillings. This research investigates the inclusion of high‐conductivity phase change material (PCM) in the borehole heat exchanger of a ground source heat pump to reduce the borehole length required and improve its coefficient of performance (COP). In the laboratory model, the borehole heat exchanger was represented by a cylindrical electrical heater having a total power of 9.216 W, operating for 1 hour while resting for 3 hours. Surrounding the heater in the annular region, either soil, PCM, or high‐conductivity PCM was used as grouting material. The annular region was surrounded by a large amount of soil enclosed in a large bin as a representation of ground soil. The high‐conductivity graphite was impregnated with the commercial PCM “PureTemp29.” Results from the experiments revealed that the PCM is able to decrease the temperature fluctuations in the annular and soil regions, while graphite increases the thermal conductivity of the annular region and hence increases the rate of heat dissipation from the heater to the soil surrounding it. The maximum COP values of a ground source heat pump calculated assuming ideal reversed Carnot cycle for cooling mode showed an increase of approximately 81% with PCM and by 112% with graphite‐enhanced PCM.     

Thermal buffering effect of a packaging design with microencapsulated phase change material

Temperature fluctuations during storage and transportation are the most important factors affecting quality and shelf life of food products. Phase change materials (PCM) with their isothermal characteristics are used to control temperature in various thermal operations. In this study, octanoic acid as PCM candidate was used in a packaging material design for thermal control of a food product. The PCM candidate was microencapsulated in different shell materials in our laboratory. Among the synthesized microcapsules, microencapsulated PCM (mPCM) (ΔHm = 42.9 J/g) with styrene polymer as the shell material was selected based on its properties of being cost effective and compatibility with human health. Thermal buffering effect of PCM in bulk and microencapsulated forms was tested in a packaging design with special PCM pockets. Results showed that packages with mPCM and bulk PCM provided 8.8 and 6 hours of thermal buffering effect for 160 g of chocolate compared with the package without PCM (reference package).     

A mixed integer programming model for a closed-loop supply-chain network

In this paper, a new mixed integer mathematical model for a closed-loop supply-chain network that includes both forward and reverse flows with multi-periods and multi-parts is proposed. The proposed model guarantees the optimal values of transportation amounts of manufactured and disassembled products in a closed-loop supply chain while determining the location of plants and retailers. Finally, computational results are presented for a number of scenarios to show and validate the applicability of the model.     

Heating and cooling of a hospital using solar energy coupled with seasonal thermal energy storage in an aquifer

A system is being designed, using solar energy in combination with Aquifer Thermal Energy Storage (ATES), that will conserve a major part of the oil and electricity used for heating or cooling the Cukurova University, Balcali Hospital in Adana, Turkey. The general objective of the system is to provide heating and cooling to the hospital by storing solar heat underground in summer and cold in winter. As the main source of cold energy, ventilation air at the hospital and surface water from the nearby Seyhan Lake will be used.     

A genetic algorithm approach for multi-objective optimization of supply chain networks

Supply chain network (SCN) design is to provide an optimal platform for efficient and effective supply chain management. It is an important and strategic operations management problem in supply chain management, and usually involves multiple and conflicting objectives such as cost, service level, resource utilization, etc. This paper proposes a new solution procedure based on genetic algorithms to find the set of Pareto-optimal solutions for multi-objective SCN design problem. To deal with multi-objective and enable the decision maker for evaluating a greater number of alternative solutions, two different weight approaches are implemented in the proposed solution procedure. An experimental study using actual data from a company, which is a producer of plastic products in Turkey, is carried out into two stages. While the effects of weight approaches on the performance of proposed solution procedure are investigated in the first stage, the proposed solution procedure and simulated annealing are compared according to quality of Pareto-optimal solutions in the second stage.     

Mixed model disassembly line balancing problem with fuzzy goals

The collection of used products is the driving force of remanufacturing systems and enterprises can gain significant economic, technical and social benefits from recycling. All products are disassembled up to some level in remanufacturing systems. The best way to disassemble returned products is valid by a well-balanced disassembly line. In this paper, a mixed integer programming (MIP) model is proposed for a mixed model disassembly line balancing (MMDLB) problem with multiple conflicting objectives: (1) minimising the cycle time, (2) minimising the number of disassembly workstations and (3) providing balanced workload per workstation. In most real world MMDLB problems, the targeted goals of decision makers are frequently imprecise or fuzzy because some information may be incomplete and/or unavailable over the planning horizon. This study is the first in the literature to offer the binary fuzzy goal programming (BFGP) and the fuzzy multi-objective programming (FMOP) approaches for the MMDLB problem in order to take into account the vague aspirations of decision makers. An illustrative example based on two industrial products is presented to demonstrate the validity of the proposed models and to compare the performances of the BFGP and the FMOP approaches.