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Zusammenfassung Es wird über Sulfonamidbefunde in Nieren und Muskulatur geschlachteter Tiere (36 Rinder, 3 Schweine) berichtet. Gewebeproben wurden vor und nach der Gewebefraktionierung mit einem durch Trimethoprim modifizierten Hemmstofftest (Teststamm:Bac. subtilis BGA) auf Hemmaktivität sowie mit einer dünnschichtchromatographischen Methode (DC) auf Sulfonamide untersucht. Als Indikatorreakti on für die Darstellung der Sulfonamide diente deren Derivatisierung mit Fluorescamin (Fluram) nach zweidimensionaler Trennung. Insgesamt wurden 11 verschiedene Sulfonamide identifiziert. An Hand von Dosis-Wirkungsbeziehungen wurde gezeigt, daß das mit Trimethoprim modifizierte Testsystem mit Ausnahme der enteral angewandten und kaum absorbierbaren Substanzen allen sonst untersuchten Sulfonamiden gegenüber mittel- bis hochgradig sensitiv ist. Hierbei liegt die minimale Hemmkonzentration im Agarlochtest (50 l) zwischen 10 und 25 Nanogramm je Sulfonamid. Der Vergleich der Ergebnisse von mikrobiologischem Test und chemischer Analyse zeigte bei dem vorliegenden Untersuchungsmaterial, daß knapp 30% der mit dem modifizierten Hemmstofftest positiv reagierenden Nierenproben aufgrund des DC-Befundes nicht als sulfonamidhaltig anzusehen waren, während umgekehrt bei den Muskelproben nahezu 30% der mit Hilfe der DC als sulfonamidhaltig erkannten Proben durch den mikrobiologischen Test nicht erfaßt wurden.
Analysis of sulfonamides in tissues of slaughtered animals. Comparison of results from a microbiological test and from thin layer chromatographic analysis after derivatization with fluorescamin
Summary Sulfonamide residues in kidneys and muscles of slaughtered animals (36 cattle, 3 pigs) are reported. Tissue samples were examined prior to and after separation first for inhibitory activity by an inhibition test modified by addition of trimethoprim and subsequently for sulfonamides by thin layer chromatography (TLC). Sulfonamides were demonstrated by derivatisation with fluorescamine (Fluram) after two dimensional separation. A total of 11 different sulfonamides was identified. It could be demonstrated by dosage-activity relationships that the test system modified with trimethoprim was moderately to highly sensitive to all examined sulfonamides with the exception of the enterally applied and hardly absorbable substances. In this test (agar diffusion test, 50 l per well) the minimal inhibotory concentration is between 10 and 25 ng for each of the sulfonamides. Comparing the microbiological test with the chemical analysis it could be demonstrated that nearly 30% of the kidney samples which were positive in the modified inhibition test did not contain sulfonamides according to interpretation of the TLC. On the other hand nearly 30% of the muscle samples containing sulfonamides (found by TLC) did not react to the microbiological test.
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Electron traps in low-temperature-grown ~40-nm-thick GaAs layers containing nanometer As-Sb clusters have been studied using deep-level transient spectroscopy. Measurements at various bias voltages and small-amplitude filling pulses have allowed the identification of two groups (T1 and T2) of traps with substantially different thermal electron emission rates. It is shown that the density of traps T2 (with an activation energy of 0.56 ± 0.04 eV and electron capture cross section of 2 × 10?13?10?12cm2) is ~2 × 1012cm?2, while the density of traps T1 (0.44 ± 0.02 eV and 2 × 10?14?10?13 cm2, respectively) is ten times lower. It is assumed that, according to the existence of the two cluster groups observed in the layers under study, traps T2 are associated with clusters 4–7 nm in diameter and traps T1, with clusters up to ~20 nm in diameter.  相似文献   
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Device degradation modelling is more and more important for reliable circuit design. On MOSFET, the threshold voltage drift in time can lead to circuit performance degradation. In this study, VT shift due to Hot Carrier Injection stress is accelerated on small width devices. VT matching is also degraded during stress as a function of VT deterioration. This width dependence allows explaining gate voltage matching behavior in the sub-threshold area used in low power analog applications.  相似文献   
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Electron-microscopy studies of GaAs structures grown by the method of molecular-beam epitaxy and containing arrays of semiconductor InAs quantum dots and metallic As quantum dots are performed. An array of InAs quantum dots is formed using the Stranski-Krastanow mechanism and consists of five layers of vertically conjugated quantum dots divided by a 5-nm-thick GaAs spacer layer. The array of As quantum dots is formed in an As-enriched GaAs layer grown at a low temperature above an array of InAs quantum dots using postgrowth annealing at temperatures of 400–600°C for 15 min. It is found that, during the course of structure growth near the InAs quantum dots, misfit defects are formed; these defects are represented by 60° or edge dislocations located in the heterointerface plane of the semiconductor quantum dots and penetrating to the surface through a layer of “low-temperature” GaAs. The presence of such structural defects leads to the formation of As quantum dots in the vicinity of the middle of the InAs conjugated quantum dots beyond the layer of “low-temperature” GaAs.  相似文献   
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Mechanisms of InGaAlAs solid solution decomposition stimulated by a purposely deposited layer of InAs quantum dots are studied. Decomposition of the solid solution results in an increase in the effective quantum dot size and the shift of the photoluminescence line to as far as 1.3 μm. When aluminum atoms are added to the solid solution, the effect of In atom “conservation” within the dots is observed, which also causes an increase in the effective dot size. __________ Translated from Fizika i Tekhnika Poluprovodnikov, Vol. 34, No. 3, 2000, pp. 330–333. Original Russian Text Copyright ? 2000 by Tsatsul’nikov, Volovik, Bedarev, Zhukov, Kovsh, Ledentsov, Maksimov, Maleev, Musikhin, Ustinov, Bert, Kop’ev, Bimberg, Alferov.  相似文献   
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Summary Analytical results are given for whey powders prepared on a commercial or semi-commercial scale by three companies. Altogether, five preparations enriched in β-lactoglobulin, four whey protein isolates and a fraction enriched in α-lactalbumin were analyzed for protein composition, including %β-lactoglobulin, α-lactalbumin, bovine serum albumin, casein (glyco) macropeptide and the main triglycerides. Protein composition was determined by high pressure gel permeation and reversed phase liquid chromatography and by capillary zone electrophoresis. The extent of modification of the native β-lactoglobulin structure was also measured through the degree of lactosylation and the fraction of accessible free sulphydryl groups. One significant finding was that the calculated recovery of protein following quantitation of the chromatogram or electropherogram was seldom above 90% and occasionally below 60% of that loaded onto the column or capillary, raising doubts as to the reliability of the analytical results. Extrapolation by linear regression to 100% recovery allowed estimates to be made of the true β-lactoglobulin composition of the samples. The nine samples could be placed into three distinct groups with estimated true β-lactoglobulin weight % of 70.9 ± 1.1, 62.0 ± 3.4 and 39.5 ± 4.9. Physico-chemical properties of the group of samples are reported elsewhere (Holt et al ., 1999).  相似文献   
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The validity of traffic intensity near the home as an estimate for the personal long-term exposure to traffic-related air pollution in an adult population was tested. Personal and near-home outdoor exposure to PM2.5, soot, NO, NO2, and NOx was monitored four to five times during 48 h periods in older adults. A group of 23 participants lived in high traffic intensity streets (>10000 vehicles/(24 h)), and 22 lived in low traffic intensity streets. The relation between average personal exposure and traffic intensity at the residential address was explored by taking indoor sources into account. Large differences in the measured outdoor concentrations between locations in high traffic and low traffic intensity streets were found for soot (68%), NO (127%), and NOx (35%). Differences were smaller for PM2.5 (14%) and NO2 (22%). Slightly elevated ratios were found for personal exposure to soot (1.15; 95% confidence interval (CI), 1.01-1.30)when comparing adults living in high traffic intensity streets with adults living in low traffic intensity streets. For NO, increased personal exposure (1.16) was seen for the same comparison, but this difference failed to reach statistical significance (CI, 0.80-1.66). Traffic intensity on the street of residence predicted personal exposure to soot but not to PM2.5 or nitrogen oxides. Traffic intensity may not correlate well to personal exposure and accordingly substantial misclassification of exposure may occur when traffic intensity is used as an exposure indicator in epidemiological studies. Time spent in traffic and spending time outdoors were associated with increased personal exposure of soot and PM2.5, but not NOx.  相似文献   
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